Author Correction: High divergence of human astrovirus genotypes circulating in pediatric patients hospitalized with acute gastroenteritis in Chiang Mai, Thailand, 2017–2020

Abstract ABSTRCT Human astrovirus (HAstV) is one of the common causes of acute gastroenteritis in children. The investigation of the molecular epidemiology of HAstV is essential for monitoring the emergence and/or re-emergence of new HAstV genotypes, as well as understanding the evolution of HAstV genotypes circulating in children suffering from acute gastroenteritis. The present study aimed to investigate the prevalence and distribution of HAstV genotypes circulating in children < 5 years of age hospitalized with acute gastroenteritis in Chiang Mai, Thailand during 2017 to 2020. A total of 1,500 fecal specimens collected from children diagnosed with acute gastroenteritis during 2017 to 2020 were screened for HAstV by RT-PCR and identified by nucleotide sequencing. The obtained sequences were compared with the reference sequences retrieved from GenBank database and analysed by MEGA X software to assign their genotypes. Of 1,500 fecal samples, 39 (2.6%) were positive for HAstV. Of these, both classic and novel HAstV genotypes, including classic HAstV1-HAstV5, novel HAstV-MLB1, MLB2, and novel HAstV-VA2, were detected in this study. The data in this study revealed a wide variety of HAstV genotypes circulating in pediatric patients admitted to the hospitals with acute gastroenteritis in Chiang Mai, Thailand during 2017 to 2020.

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Emergence of Multiple Novel Inter-Genotype Recombinant Strains of Human Astroviruses Detected in Pediatric Patients With Acute Gastroenteritis in Thailand

Frontiers in Microbiology ◽

10.3389/fmicb.2021.789636 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hongyu Wei ◽

Pattara Khamrin ◽

Kattareeya Kumthip ◽

Arpaporn Yodmeeklin ◽

Niwat Maneekarn

Keyword(s):

Acute Gastroenteritis ◽

Pediatric Patients ◽

Junction Region ◽

Chiang Mai ◽

Recombinant Strains ◽

Human Astroviruses ◽

Human Astrovirus ◽

Reference Sequences ◽

Recombination Breakpoints

Objective: Human astrovirus (HAstV) is recognized as an important cause of acute gastroenteritis in children. Recombination between different genotypes of HAstV can contribute to diversity and evolution of the virus. This study aimed to investigate the emergence of HAstV recombinant strains in pediatric patients hospitalized with acute gastroenteritis in Chiang Mai, Thailand, spanning 2011–2020.Methods: A total of 92 archival HAstV strains collected from pediatric patients with acute gastroenteritis during 2011–2020 were further characterized to identify the recombinant strains. The ORF1b and ORF2 junction region of each strain was amplified and sequenced. The obtained sequences were analyzed in comparison with the reference sequences retrieved from GenBank database. Their genotypes were assigned using MEGA X software based on the partial ORF1b (RdRp) and ORF2 (capsid) regions, and the recombination breakpoints of recombinant strains were determined by SimPlot and RDP4 analyses.Results: Five inter-genotype recombinant strains with three recombination patterns of ORF1b/ORF2 of classic HAstV, HAstV8/HAstV1, HAstV8/HAstV3, and HAstV3/HAstV2, were detected. The recombination breakpoints of all strains were located at the 3′-end region of ORF1b close to the ORF1b/ORF2 junction.Conclusion: Several novel inter-genotype recombinant strains of classic HAstV genotypes were detected in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand, during the period of 10 years from 2011 to 2020.

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High divergence of human astrovirus genotypes circulating in pediatric patients hospitalized with acute gastroenteritis in Chiang Mai, Thailand, 2017–2020

Scientific Reports ◽

10.1038/s41598-021-02745-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Hongyu Wei ◽

Pattara Khamrin ◽

Kattareeya Kumthip ◽

Arpaporn Yodmeeklin ◽

Niwat Maneekarn

Keyword(s):

Phylogenetic Analysis ◽

Molecular Epidemiology ◽

Acute Gastroenteritis ◽

Pediatric Patients ◽

Rt Pcr ◽

Chiang Mai ◽

Common Causes ◽

Human Astrovirus ◽

Partial Rdrp ◽

The Common

AbstractHuman astrovirus (HAstV) is one of the common causes of acute gastroenteritis in children. The investigation of molecular epidemiology of HAstV is essential for monitoring the emergence and/or re-emergence of new HAstV genotypes, as well as understanding the evolution of HAstV circulating in children suffering from acute gastroenteritis. The present study aimed to investigate the prevalence and distribution of HAstVs strains circulating in children hospitalized with acute gastroenteritis in Chiang Mai, Thailand during 2017–2020. A total of 1500 fecal specimens collected from children with acute gastroenteritis were screened for HAstV by RT-PCR that targeted the partial RdRp in ORF1b and strains were characterized by sequencing and phylogenetic analysis. Of the 1500 fecal samples, 39 (2.6%) were positive for HAstV. Of these, both classic and novel HAstV genotypes, including classic HAstV1–HAstV5, novel HAstV-MLB1, MLB2, and HAstV-VA2, were detected. The data in this study revealed a high divergence of HAstV genotypes circulating in pediatric patients admitted to the hospitals with acute gastroenteritis in Chiang Mai, Thailand during 2017–2020.

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Human bocavirus genotypes 1, 2, and 3 circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand, 2012–2018

Journal of Infection and Public Health ◽

10.1016/j.jiph.2020.12.007 ◽

2021 ◽

Vol 14 (2) ◽

pp. 179-186

Author(s):

Nattika Nantachit ◽

Pakawat Kochjan ◽

Pattara Khamrin ◽

Kattareeya Kumthip ◽

Niwat Maneekarn

Keyword(s):

Acute Gastroenteritis ◽

Pediatric Patients ◽

Human Bocavirus ◽

Chiang Mai

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Enterovirus infections in pediatric patients hospitalized with acute gastroenteritis in Chiang Mai, Thailand, 2015–2018

PeerJ ◽

10.7717/peerj.9645 ◽

2020 ◽

Vol 8 ◽

pp. e9645

Author(s):

Kitsakorn Rojjanadumrongkul ◽

Kattareeya Kumthip ◽

Pattara Khamrin ◽

Nuthapong Ukarapol ◽

Hiroshi Ushijima ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Pediatric Patients ◽

Nucleotide Sequencing ◽

Rt Pcr ◽

Chiang Mai ◽

High Prevalence ◽

Genotype Diversity ◽

The Common ◽

Viral Protein 1 ◽

Children Under 5

Background Infection with viruses especially rotavirus, norovirus, astrovirus, and adenovirus has been known to be a major cause of acute gastroenteritis in children under 5 years of age globally, particularly in developing countries. Also, some genotypes of enteroviruses (EVs) have been reported to be associated with gastroenteritis. This study is aimed to investigate the prevalence and genotype diversity of EV in children admitted to hospitals with acute gastroenteritis. Methods A total of 1,736 fecal specimens were collected from children hospitalized with diarrhea in Chiang Mai, Thailand from 2015 to 2018. All specimens were tested for the presence of EV by RT-PCR of the 5′ untranslated region. The genotypes of EV were further identified by nucleotide sequencing and phylogenetic analysis of the viral protein 1 (VP1) gene. Results EV was detected in 154 out of 1,736 specimens (8.9%) throughout the study period. The prevalence of EV detected in 2015, 2016, 2017, and 2018 was 7.2%, 9.0%, 11.2%, and 8.6%, respectively. EV was detected all year round with a high prevalence during rainy season in Thailand. Overall, 37 genotypes of EV were identified in this study. Among these, coxsackievirus (CV)-A24 and CV-B5 (7.5% each), and EV-C96 (6.8%) were the common genotypes detected. Conclusion This study demonstrates the prevalence, seasonal distribution, and genotype diversity of EV circulating in children hospitalized with acute gastroenteritis in Chiang Mai, Thailand during the period 2015 to 2018.

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Human Astrovirus Outbreak in a Daycare Center and Propagation among Household Contacts

Viruses ◽

10.3390/v13061100 ◽

2021 ◽

Vol 13 (6) ◽

pp. 1100

Author(s):

Ignacio Parrón ◽

Elsa Plasencia ◽

Thais Cornejo-Sánchez ◽

Mireia Jané ◽

Cristina Pérez ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Age Groups ◽

Rt Pcr ◽

Daycare Center ◽

Infected People ◽

Conditioning Factors ◽

Household Contacts ◽

Human Astrovirus ◽

Asymptomatic Infections ◽

Clinical Cases

We investigated an outbreak of acute gastroenteritis due to human astrovirus in a daycare center, describing the transmission mechanism, the most affected age groups, conditioning factors and the extent of the outbreak among household contacts of the daycare center attenders. Data were collected from persons exposed at the daycare center and their home contacts. Fecal samples from affected and non-affected daycare center attenders were analyzed for viruses causing acute gastroenteritis by RT-PCR. The percentage of households affected and the attack rates (AR) were calculated. The attack rates were compared using the rate ratio (RR) with 95% confidence intervals. Information was obtained from 245 people (76 attenders and 169 contacts) of whom 49 were clinical cases. Five HAstV-4, two HAstV-8 and three non-typable HAstV cases were identified (six from clinical cases and four from asymptomatic infected people). The global AR was 20% (41.2% in children aged < 2 years). Data were obtained from 67 households: 20 households of affected attenders and 47 of non-affected attendees. Household contacts of affected attenders had a higher AR (74.3%) than that of non-affected attendees (2.4%). We found asymptomatic infections amongst daycare attendees. The transmission of HAstV during the outbreak was not limited to the daycare center but extended to household contacts of both affected and non-affected attenders.

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Genetic Diversity of Norovirus and Sapovirus in Hospitalized Infants with Sporadic Cases of Acute Gastroenteritis in Chiang Mai, Thailand

Journal of Clinical Microbiology ◽

10.1128/jcm.42.3.1305-1307.2004 ◽

2004 ◽

Vol 42 (3) ◽

pp. 1305-1307 ◽

Cited By ~ 103

Author(s):

G. S. Hansman ◽

K. Katayama ◽

N. Maneekarn ◽

S. Peerakome ◽

P. Khamrin ◽

...

Keyword(s):

Genetic Diversity ◽

Acute Gastroenteritis ◽

Chiang Mai ◽

Sporadic Cases

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Changing pattern of prevalence, genetic diversity, and mixed infections of viruses associated with acute gastroenteritis in pediatric patients in New Delhi, India

Journal of Medical Virology ◽

10.1002/jmv.24980 ◽

2017 ◽

Vol 90 (3) ◽

pp. 469-476 ◽

Cited By ~ 8

Author(s):

Shipra Gupta ◽

Anuja Krishnan ◽

Sumit Sharma ◽

Praveen Kumar ◽

Satinder Aneja ◽

...

Keyword(s):

Genetic Diversity ◽

Acute Gastroenteritis ◽

Pediatric Patients ◽

Mixed Infections ◽

New Delhi

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Vaccinia Virus Recombinant Expressing an 87-Kilodalton Polyprotein That Is Sufficient To Form Astrovirus-Like Particles

Journal of Virology ◽

10.1128/jvi.77.16.9094-9098.2003 ◽

2003 ◽

Vol 77 (16) ◽

pp. 9094-9098 ◽

Cited By ~ 19

Author(s):

Rosa M. Dalton ◽

Esperanza P. Pastrana ◽

Alicia Sánchez-Fauquier

Keyword(s):

Vaccinia Virus ◽

Acute Gastroenteritis ◽

Genomic Rna ◽

Virus Like Particles ◽

Virus Recombinant ◽

Human Astrovirus ◽

First Time ◽

Vaccinia Virus Recombinant

ABSTRACT Human astrovirus is an important cause of acute gastroenteritis. We have generated, for the first time, a vaccinia virus recombinant expressing the astrovirus 87-kDa structural polyprotein. The results demonstrate that this expression results in the formation of virus-like particles in the absence of other astrovirus proteins and genomic RNA. The purified trypsin-activated virus-like particles strongly resemble the complete astrovirus particles.

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