scholarly journals Population differences and domestication effects on mating and remating frequencies in Queensland fruit fly

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Khandaker Asif Ahmed ◽  
Heng Lin Yeap ◽  
Gunjan Pandey ◽  
Siu Fai Lee ◽  
Phillip W. Taylor ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Reproductive Strategies ◽  
Natural Variation ◽  
Captive Breeding ◽  
Strain Differences ◽  
Fruit Fly ◽  
Inhibitory Effect ◽  
Pest Species ◽  
Bactrocera Tryoni ◽  
Male Genotype

AbstractFemales of many insect species are unreceptive to remating for a period following their first mating. This inhibitory effect may be mediated by either the female or her first mate, or both, and often reflects the complex interplay of reproductive strategies between the sexes. Natural variation in remating inhibition and how this phenotype responds to captive breeding are largely unexplored in insects, including many pest species. We investigated genetic variation in remating propensity in the Queensland fruit fly, Bactrocera tryoni, using strains differing in source locality and degree of domestication. We found up to threefold inherited variation between strains from different localities in the level of intra-strain remating inhibition. The level of inhibition also declined significantly during domestication, which implied the existence of genetic variation for this trait within the starting populations as well. Inter-strain mating and remating trials showed that the strain differences were mainly due to the genotypes of the female and, to a lesser extent, the second male, with little effect of the initial male genotype. Implications for our understanding of fruit fly reproductive biology and population genetics and the design of Sterile Insect Technique pest management programs are discussed.

scholarly journals Efficiency of RNA interference is improved by knockdown of dsRNA nucleases in tephritid fruit flies

Open Biology ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 190198 ◽  
Author(s):  
Alison Tayler ◽  
Daniel Heschuk ◽  
David Giesbrecht ◽  
Jae Yeon Park ◽  
Steve Whyard
Keyword(s):  
Rna Interference ◽  
Fruit Fly ◽  
Target Cells ◽  
Pest Species ◽  
Melanin Synthesis ◽  
Bactrocera Tryoni ◽  
Double Stranded Rna ◽  
Rnai Efficiency ◽  
Gene Transcripts ◽  
Tephritid Fruit Flies

RNA interference (RNAi) in insects is routinely used to ascertain gene function, but also has potential as a technology to control pest species. For some insects, such as beetles, ingestion of small quantities of double-stranded RNA (dsRNA) is able to knock down a targeted gene's expression. However, in other species, ingestion of dsRNA can be ineffective owing to the presence of nucleases within the gut, which degrade dsRNA before it reaches target cells. In this study, we observed that nucleases within the gut of the Queensland fruit fly ( Bactrocera tryoni ) rapidly degrade dsRNA and reduce RNAi efficacy. By complexing dsRNA with liposomes within the adult insect's diet, RNAi-mediated knockdown of a melanin synthesis gene, yellow , was improved significantly, resulting in strong RNAi phenotypes. RNAi efficiency was also enhanced by feeding both larvae and adults for several days on dsRNAs that targeted two different dsRNase gene transcripts. Co-delivery of both dsRNase-specific dsRNAs and yellow dsRNA resulted in almost complete knockdown of the yellow transcripts. These findings show that the use of liposomes or co-feeding of nuclease-specific dsRNAs significantly improves RNAi inhibition of gene expression in B. tryoni and could be a useful strategy to improve RNAi-based control in other insect species.

scholarly journals Independent natural genetic variation of punishment- versus relief-memory

2016 ◽  
Vol 12 (12) ◽  
pp. 20160657 ◽  
Author(s):  
Mirjam Appel ◽  
Claus-Jürgen Scholz ◽  
Samet Kocabey ◽  
Sinead Savage ◽  
Christian König ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Natural Variation ◽  
Fruit Fly ◽  
Inbred Strains ◽  
Adaptive Behaviour ◽  
Clinical Implications ◽  
Natural Genetic Variation ◽  
Study Case ◽  
Positive Valence

A painful event establishes two opponent memories: cues that are associated with pain onset are remembered negatively, whereas cues that coincide with the relief at pain offset acquire positive valence. Such punishment- versus relief-memories are conserved across species, including humans, and the balance between them is critical for adaptive behaviour with respect to pain and trauma. In the fruit fly, Drosophila melanogaster as a study case, we found that both punishment- and relief-memories display natural variation across wild-derived inbred strains, but they do not covary, suggesting a considerable level of dissociation in their genetic effectors. This provokes the question whether there may be heritable inter-individual differences in the balance between these opponent memories in man, with potential psycho-clinical implications.

scholarly journals Ecological Drivers and Sex-Based Variation in Body Size and Shape in the Queensland Fruit Fly, Bactrocera tryoni (Diptera: Tephritidae)

Insects ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 390
Author(s):  
Yufei Zhou ◽  
Juanita Rodriguez ◽  
Nicole Fisher ◽  
Renee A. Catullo
Keyword(s):  
Body Size ◽  
Fluctuating Asymmetry ◽  
Morphological Traits ◽  
Geographic Distance ◽  
Fruit Fly ◽  
Wing Shape ◽  
The Body ◽  
Economic Losses ◽  
Pest Species ◽  
Bactrocera Tryoni

The Queensland fruit fly (Bactrocera tryoni; Q-fly) is an Australian endemic horticultural pest species, which has caused enormous economic losses. It has the potential to expand its range to currently Q-fly-free areas and poses a serious threat to the Australian horticultural industry. A large number of studies have investigated the correlation between environmental factors and Q-fly development, reproduction, and expansion. However, it is still not clear how Q-fly morphological traits vary with the environment. Our study focused on three morphological traits (body size, wing shape, and fluctuating asymmetry) in Q-fly samples collected from 1955 to 1965. We assessed how these traits vary by sex, and in response to latitude, environmental variables, and geographic distance. First, we found sexual dimorphism in body size and wing shape, but not in fluctuating asymmetry. Females had a larger body size but shorter and wider wings than males, which may be due to reproductive and/or locomotion differences between females and males. Secondly, the body size of Q-flies varied with latitude, which conforms to Bergmann’s rule. Finally, we found Q-fly wing shape was more closely related to temperature rather than aridity, and low temperature and high aridity may lead to high asymmetry in Q-fly populations.

scholarly journals Comparative Trap Catches of Male Bactrocera, Dacus, and Zeugodacus Fruit Flies (Diptera: Tephritidae) With Four Floral Phenylbutanoid Lures (Anisyl Acetone, Cue-Lure, Raspberry Ketone, and Zingerone) in Queensland, Australia

2020 ◽  
Vol 49 (4) ◽  
pp. 815-822 ◽  
Author(s):  
Jane E Royer ◽  
Keng Hong Tan ◽  
David G Mayer
Keyword(s):  
Pest Management ◽  
Fruit Fly ◽  
Quantitative Comparison ◽  
Pest Species ◽  
Bactrocera Tryoni ◽  
Raspberry Ketone ◽  
Relative Attractiveness ◽  
Similar Range ◽  
Multiple Species ◽  
Trap Catches

Abstract The male fruit fly attractants, cue-lure (CL) and raspberry ketone (RK), are important in pest management. These volatile phenylbutanoids occur in daciniphilous Bulbophyllum Thouar (Orchidaceae: Asparagales) orchids, along with zingerone (ZN) and anisyl acetone (AA). While these four compounds attract a similar range of species, their relative attractiveness to multiple species is unknown. We field tested these compounds in two fruit fly speciose locations in north Queensland, Australia (Lockhart and Cairns) for 8 wk. Of 16 species trapped in significant numbers, 14 were trapped with CL and RK, all in significantly greater numbers with CL traps than RK traps (at least in higher population locations). This included the pest species Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) (CL catches ca. 5× > RK), Bactrocera neohumeralis (Hardy) (Diptera: Tephritidae) and Bactrocera bryoniae (Tryon) (Diptera: Tephritidae) (CL catches ca. 3× > RK), and Bactrocera frauenfeldi (Schiner) (Diptera: Tephritidae) (in Cairns—CL catches ca. 1.6× > RK). Seven species were trapped with AA, and all were also caught in CL and RK traps in significantly greater numbers, with the exception of B. frauenfeldi. For this species, catches were not statistically different with CL, RK, and AA in Lockhart, and RK and AA in Cairns. Seven species were trapped with ZN, two at this lure only, and the remainder also with CL or RK but in significantly greater numbers. This is the first quantitative comparison of the relative attractiveness of CL, RK, AA, and ZN against multiple species, and supports the long-held but untested assumption that CL is broadly more attractive lure than RK.

Genetic variation between strains of the Mediterranean fruit fly, Ceratitis capitata, detected by DNA fingerprinting

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 528-533 ◽  
Author(s):  
David S. Haymer ◽  
Donald O. Mcinnis ◽  
Loretta Arcangeli
Keyword(s):  
Genetic Variation ◽  
Dna Fingerprinting ◽  
Restriction Fragment ◽  
Ceratitis Capitata ◽  
Geographic Origin ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Great Promise ◽  
Pest Species ◽  
The Mediterranean

DNA fingerprinting has been used to detect genetic variation in the Mediterranean fruit fly, Ceratitis capitata. Three different probes have been identified that can be used to detect DNA restriction fragment length polymorphisms between strains of this species. The strains used in this study differ only in terms of their geographic origin or genetic background. One of the probes used is the bacteriophage vector M13, and the other two are repetitive sequences derived from the medfly genome based on a weak homology to M13. Within a strain, each probe produces a consistent restriction fragment profile that is not affected by the method or timing of DNA extraction. Between strains, when M13 is used as a probe, an average of 10% of the observable bands are polymorphic. Use of the medfly genomic sequences as a probe increases the proportion of polymorphic bands between strains up to 30%. The fact that genetic differences between even such closely related strains can be reliably detected by this method holds great promise for studies of insect pests including the ability to monitor the movements of pest species, determining the extent of genetic variation in pest populations, and in making identifications from otherwise unidentifiable material.Key words: genetic variation, Ceratitis capitata, DNA fingerprinting.

scholarly journals Inferring Modes of Colonization for Pest Species Using Heterozygosity Comparisons and a Shared-Allele Test

Genetics ◽  
2003 ◽  
Vol 163 (2) ◽  
pp. 823-831
Author(s):  
J A Sved ◽  
H Yu ◽  
B Dominiak ◽  
A S Gilchrist
Keyword(s):  
Distance Measure ◽  
Fruit Fly ◽  
Small Samples ◽  
Pest Species ◽  
Long Distance ◽  
Resampling Methods ◽  
Shared Allele ◽  
The Core ◽  
Direct Sampling ◽  
Long Distance Movement

Abstract Long-range dispersal of a species may involve either a single long-distance movement from a core population or spreading via unobserved intermediate populations. Where the new populations originate as small propagules, genetic drift may be extreme and gene frequency or assignment methods may not prove useful in determining the relation between the core population and outbreak samples. We describe computationally simple resampling methods for use in this situation to distinguish between the different modes of dispersal. First, estimates of heterozygosity can be used to test for direct sampling from the core population and to estimate the effective size of intermediate populations. Second, a test of sharing of alleles, particularly rare alleles, can show whether outbreaks are related to each other rather than arriving as independent samples from the core population. The shared-allele statistic also serves as a genetic distance measure that is appropriate for small samples. These methods were applied to data on a fruit fly pest species, Bactrocera tryoni, which is quarantined from some horticultural areas in Australia. We concluded that the outbreaks in the quarantine zone came from a heterogeneous set of genetically differentiated populations, possibly ones that overwinter in the vicinity of the quarantine zone.

Dacus tryoni. [Distribution map].

1960 ◽  
Author(s):  
Keyword(s):  
Geographical Distribution ◽  
New South ◽  
New South Wales ◽  
South Australia ◽  
Fruit Fly ◽  
Distribution Map ◽  
Bactrocera Tryoni ◽  
South Wales ◽  
New Distribution

Abstract A new distribution map is provided for Dacus tryoni[Bactrocera tryoni] (Frogg.) (Dipt., Trypetidae) (Queensland Fruit-fly) Hosts: Many deciduous and subtropical fruits. Information is given on the geographical distribution in AUSTRALIA, New South Wales, Queensland, South Australia, Victoria.

Sign in / Sign up

Export Citation Format

Share Document