scholarly journals Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Ebrahim Asadi Zarch ◽  
Alireza Afshar ◽  
Farhad Rahmanifar ◽  
Mohammad Reza Jafarzadeh Shirazi ◽  
Mandana Baghban ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Ovarian Tissue ◽  
Three Dimensional ◽  
Corpora Lutea ◽  
3D Analysis ◽  
Two Dimensional ◽  
Tissue Samples ◽  
Adult Mice ◽  
Mature Mouse ◽  
2D And 3D

AbstractThe present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from follicular and luteal phases after cardiac perfusion. Ovarian samples were stained by a Golgi–Cox protocol. Following staining, tissues were serially sectioned for imaging. Neural filaments and ganglia were present in the ovaries. In both 2D and 3D studies, an increase in the number and area of ganglia was seen during the follicular growth. The same pattern was also seen in corpora lutea development. However, in some cases such as ratio of ganglia number to follicle area, the ratio of ganglia area to follicular area, 2D findings were different compared with the 3D results. 3D analysis of ovarian gangliogenesis showed the possible direct effect of them on folliculogenesis. Golgi–Cox staining was used in this study for 3D evaluation in non-brain tissue. The results of 3D analysis of the present study showed that, in some cases, the information provided by 2D analysis does not match the reality of ovarian neuronal function. This confirmed the importance of 3D analysis for evaluation of ovarian function.

scholarly journals Three-Dimensional and Two-Dimensional Relationships of Gangliogenesis With Folliculogenesis in Mature Mouse Ovary: A Golgi-Cox Staining Approach

2020 ◽  
Author(s):  
Mohammad Ebrahim Asadi Zarch ◽  
Alireza Afshar ◽  
Farhad Rahmanifar ◽  
Mohammad Reza Jafarzadeh Shirazi ◽  
Mandana Baghban ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Ovarian Tissue ◽  
Three Dimensional ◽  
Corpora Lutea ◽  
3D Analysis ◽  
Two Dimensional ◽  
Mouse Ovary ◽  
Tissue Samples ◽  
Adult Mice ◽  
Mature Mouse

Abstract The present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovary. Adult mice ovarian tissue samples were collected from diestrus and estrus stages after cardiac perfusion. Ovarian samples were stained by a Golgi-Cox protocol. Following staining, tissues were serially sectioned for imaging. Neural filaments and ganglia were present in the ovaries. In both 2D and 3D studies, an increase in the number and area of ganglia was seen during the follicular growth. The same pattern was also seen in corpora lutea development. However, in some cases such as ratio of ganglia number to follicle area, the ratio of ganglia area to follicular area, 2D findings were different compared with the 3D results. 3D analysis of ovarian gangliogenesis showed the possible direct effect of them on folliculogenesis. Golgi-Cox staining was used in this study for 3D evaluation in non-brain tissue. The results of 3D analysis of the present study showed that, in some cases, the information provided by 2D analysis does not match the reality of ovarian neuronal function. This confirmed the importance of 3D analysis for evaluation of ovarian function.

scholarly journals Comparison of Two- and Three-Dimensional Methods for Analysis of Trunk Kinematic Variables in the Golf Swing

2016 ◽  
Vol 32 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Aimée C. Smith ◽  
Jonathan R. Roberts ◽  
Eric S. Wallace ◽  
Pui Kong ◽  
Stephanie E. Forrester
Keyword(s):  
Three Dimensional ◽  
Axial Rotation ◽  
Golf Swing ◽  
3D Analysis ◽  
Two Dimensional ◽  
Trunk Flexion ◽  
Flexion Extension ◽  
3D Methods ◽  
2D And 3D ◽  
Factor Difference

Two-dimensional methods have been used to compute trunk kinematic variables (flexion/extension, lateral bend, axial rotation) and X-factor (difference in axial rotation between trunk and pelvis) during the golf swing. Recent X-factor studies advocated three-dimensional (3D) analysis due to the errors associated with two-dimensional (2D) methods, but this has not been investigated for all trunk kinematic variables. The purpose of this study was to compare trunk kinematic variables and X-factor calculated by 2D and 3D methods to examine how different approaches influenced their profiles during the swing. Trunk kinematic variables and X-factor were calculated for golfers from vectors projected onto the global laboratory planes and from 3D segment angles. Trunk kinematic variable profiles were similar in shape; however, there were statistically significant differences in trunk flexion (–6.5 ± 3.6°) at top of backswing and trunk right-side lateral bend (8.7 ± 2.9°) at impact. Differences between 2D and 3D X-factor (approximately 16°) could largely be explained by projection errors introduced to the 2D analysis through flexion and lateral bend of the trunk and pelvis segments. The results support the need to use a 3D method for kinematic data calculation to accurately analyze the golf swing.

scholarly journals Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kiyoshi Masuyama ◽  
Tomoaki Higo ◽  
Jong-Kook Lee ◽  
Ryohei Matsuura ◽  
Ian Jones ◽  
...  
Keyword(s):  
Heart Failure ◽  
Dilated Cardiomyopathy ◽  
Cardiac Dysfunction ◽  
Three Dimensional ◽  
Single Layer ◽  
Specific Pattern ◽  
Two Dimensional ◽  
Heart Imaging ◽  
Novel Method ◽  
2D And 3D

AbstractIn contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) manner. Here we have established a novel method to evaluate cardiomyocyte alignment in 3D using intravital heart imaging and demonstrated homogeneous alignment in DCM mice. Whilst cardiomyocytes of control mice changed their alignment by every layer in 3D and position twistedly even in a single layer, termed myocyte twist, cardiomyocytes of DCM mice aligned homogeneously both in two-dimensional (2D) and in 3D and lost myocyte twist. Manipulation of cultured cardiomyocyte toward homogeneously aligned increased their contractility, suggesting that homogeneous alignment in DCM mice is due to a sort of alignment remodelling as a way to compensate cardiac dysfunction. Our findings provide the first intravital evidence of cardiomyocyte alignment and will bring new insights into understanding the mechanism of heart failure.

scholarly journals Do 3D Face Images Capture Cues of Strength, Weight, and Height Better than 2D Face Images do?

2021 ◽  
Vol 7 (3) ◽  
pp. 209-219
Author(s):  
Iris J Holzleitner ◽  
Alex L Jones ◽  
Kieran J O’Shea ◽  
Rachel Cassar ◽  
Vanessa Fasolt ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Physical Characteristics ◽  
Two Dimensional ◽  
3D Images ◽  
3D Face ◽  
Face Images ◽  
Similar Accuracy ◽  
2D And 3D ◽  
2D Images ◽  
Better Than

Abstract Objectives A large literature exists investigating the extent to which physical characteristics (e.g., strength, weight, and height) can be accurately assessed from face images. While most of these studies have employed two-dimensional (2D) face images as stimuli, some recent studies have used three-dimensional (3D) face images because they may contain cues not visible in 2D face images. As equipment required for 3D face images is considerably more expensive than that required for 2D face images, we here investigated how perceptual ratings of physical characteristics from 2D and 3D face images compare. Methods We tested whether 3D face images capture cues of strength, weight, and height better than 2D face images do by directly comparing the accuracy of strength, weight, and height ratings of 182 2D and 3D face images taken simultaneously. Strength, height and weight were rated by 66, 59 and 52 raters respectively, who viewed both 2D and 3D images. Results In line with previous studies, we found that weight and height can be judged somewhat accurately from faces; contrary to previous research, we found that people were relatively inaccurate at assessing strength. We found no evidence that physical characteristics could be judged more accurately from 3D than 2D images. Conclusion Our results suggest physical characteristics are perceived with similar accuracy from 2D and 3D face images. They also suggest that the substantial costs associated with collecting 3D face scans may not be justified for research on the accuracy of facial judgments of physical characteristics.

Two-dimensional-Wilkins and real-time transesophageal three-dimensional scoring systems: Which one is preferred to mitral valve morphological assessment?

2021 ◽  
pp. 021849232110304
Author(s):  
Mehrnoush Toufan ◽  
Zahra Jabbary ◽  
Naser Khezerlou aghdam
Keyword(s):  
Mitral Valve ◽  
Transthoracic Echocardiography ◽  
Scoring System ◽  
Three Dimensional ◽  
Scoring Systems ◽  
Two Dimensional ◽  
Mitral Valve Area ◽  
2D And 3D ◽  
Morphological Assessment ◽  
Valve Area

Background To quantify valvular morphological assessment, some two-dimensional (2D) and three-dimensional (3D) scoring systems have been developed to target the patients for balloon mitral valvuloplasty; however, each scoring system has some potential limitations. To achieve the best scoring system with the most features and the least restrictions, it is necessary to check the degree of overlap of these systems. Also the factors related to the accuracy of these systems should be studied. We aimed to determine the correlation between the 2D Wilkins and real-time transesophageal three-dimensional (RT3D-TEE) scoring systems. Methods This cross-sectional study was performed on 156 patients with moderate to severe mitral stenosis who were candidates for percutaneous balloon valvuloplasty. To morphologic assessment of mitral valve, patients were examined by 2D-transthoracic echocardiography and RT3D-TEE techniques on the same day. Results A strong association was found between total Wilkins and total RT3D-TEE scores (r = 0.809, p < 0.001). The mean mitral valve area assessed by the 2D and 3D was 1.07 ± 0.25 and 1.03 ± 0.26, respectively, indicating a mean difference of 0.037 cm2 (p = 0.001). We found a strong correlation between the values of mitral valve area assessed by 2D and 3D techniques (r = 0.846, p < 0.001). Conclusion There is a high correlation between the two scoring systems in terms of evaluating dominant morphological features. Partially, mitral valve area overestimation in the 2D-transthoracic echocardiography and its inability to assess commissural involvement as well as its dependence on patient age were exceptions in this study.

scholarly journals Transformation Between 2D and 3D Covalent Organic Frameworks via Reversible [2+2] Cycloaddition

2020 ◽  
Author(s):  
Thaksen Jadhav ◽  
Yuan Fang ◽  
Cheng-Hao Liu ◽  
Afshin Dadvand ◽  
Ehsan Hamzehpoor ◽  
...  
Keyword(s):  
Band Structure ◽  
Absorption Edge ◽  
Room Temperature ◽  
Three Dimensional ◽  
Cross Linking ◽  
Two Dimensional ◽  
Covalent Organic Frameworks ◽  
Doping Behavior ◽  
2D Polymers ◽  
2D And 3D

We report the first transformation between crystalline vinylene-linked two-dimensional (2D) polymers and crystalline cyclobutane-linked three-dimensional (3D) polymers. Specifically, absorption-edge irradiation of the 2D poly(arylenevinylene) covalent organic frameworks (COFs) results in topological [2+2] cycloaddition cross-linking the π-stacked layers in 3D COFs. The reaction is reversible and heating to 200°C leads to a cycloreversion while retaining the COF crystallinity. The resulting difference in connectivity is manifested in the change of mechanical and electronic properties, including exfoliation, blue-shifted UV-Vis absorption, altered luminescence, modified band structure and different acid-doping behavior. The Li-impregnated 2D and 3D COFs show a significant ion conductivity of 1.8×10<sup>−4</sup> S/cm and 3.5×10<sup>−5</sup> S/cm, respectively. Even higher room temperature proton conductivity of 1.7×10<sup>-2</sup> S/cm and 2.2×10<sup>-3</sup> S/cm was found for H<sub>2</sub>SO<sub>4</sub>-treated 2D and 3D COFs, respectively.

scholarly journals The Two-dimensional and Three-dimensional T2 Weighted Imaging-based Radiomic Signatures Selected for the Preoperative Discrimination of Ovarian Borderline Tumors and Epithelial Cancer.

2021 ◽  
Author(s):  
Xuefen Liu ◽  
Tianping Wang ◽  
Guofu Zhang ◽  
Keqin Hua ◽  
Hua Jiang ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Operator Method ◽  
Two Dimensional ◽  
Borderline Tumors ◽  
Segmentation Methods ◽  
Highly Correlated ◽  
Selection Operator ◽  
2D And 3D ◽  
Testing Group ◽  
Segmentation Models

Abstract Background: Accurate discrimination between ovarian borderline tumors (BOTs) and malignancies with imaging play an important role in management.Purpose: To evaluate the ability of T2-weighted imaging (T2WI)-based radiomics to discriminate ovarian borderline tumors (BOTs) from malignancies based on two-dimensional (2D) and three-dimensional (3D) lesion segmentation methods.Methods: A total of 95 patients with pathologically proven ovarian BOTs and 101 patients with malignancies were retrospectively included in this study. We evaluated the diagnostic performance of the signatures derived from T2WI-based radiomics in their ability to differentiate between BOTs and malignancies and compared the performance differences in the 2D and 3D segmentation models. The least absolute shrinkage and selection operator method (LASSO) was used for radiomics feature selection and machine learning processing.Results: The radiomics score between BOTs and malignancies in four types of selected T2WI-based radiomics models differed significantly at the statistical level (p < 0.0001). For the classification between BOTs and malignant masses, the 2D and 3D coronal T2WI-based radiomics models yielded accuracy values of 0.79 and 0.83 in the testing group, respectively; the 2D and 3D sagittal fat-suppressed (fs) T2WI-based radiomics models yielded an accuracy of 0.78 and 0.99, respectively.Conclusion: Our results suggest that T2WI-based radiomic features were highly correlated with ovarian tumor subtype classification. 3D-sagittal MRI radiomics features may help clinicians differentiate ovarian BOTs from malignancies with high accuracy (ACC).

Contact Temperature in Dry Bearings. Three Dimensional Theory and Verification

1981 ◽  
Vol 103 (2) ◽  
pp. 243-251 ◽  
Author(s):  
A. Floquet ◽  
D. Play
Keyword(s):  
Fourier Transform ◽  
Boundary Conditions ◽  
Experimental Verification ◽  
Three Dimensional ◽  
Contact Temperature ◽  
3D Analysis ◽  
New Work ◽  
Two Dimensional ◽  
Dimensional Theory ◽  
Infra Red

Boundary conditions were arbitrarily specified in an earlier two dimensional (2D) analysis of contact temperature. In this new work a general three dimensional (3D) Fourier transform solution is obtained from which for specific cases, the boundary conditions can be estimated. Further, experimental verification of 3D analysis was performed using infra-red technique.

Evaluation of Residual Stresses in Small Diameter Stainless Steel Pipe Welds by Two-Dimensional and Three-Dimensional Finite Element Analyses

2014 ◽  
Author(s):  
Francis H. Ku ◽  
Trevor G. Hicks ◽  
William R. Mabe ◽  
Jason R. Miller
Keyword(s):  
Residual Stress ◽  
Finite Element ◽  
Stainless Steel ◽  
Residual Stresses ◽  
Three Dimensional ◽  
Steel Pipe ◽  
3D Analysis ◽  
Two Dimensional ◽  
Finite Element Analyses ◽  
Stainless Steel Pipe

Two-dimensional (2D) and three-dimensional (3D) weld-induced residual stress finite element analyses have been performed for 2-inch Schedule 80 Type-304 stainless steel pipe sections joined by a multi-layer segmented-bead pipe weld. The analyses investigate the similarities and differences between the two modeling approaches in terms of residual stresses and axial shrinkage induced by the pipe weld. The 2D analyses are of axisymmetric behavior and evaluate two different pipe end constraints, namely fixed-fixed and fixed-free, while the 3D analysis approximates the non-axisymmetric segmented welding expected in production, with fixed-free pipe end constraints. Based on the results presented, the following conclusions can be drawn. The welding temperature contour results between the 2D and 3D analyses are very similar. Only the 3D analysis is capable of simulating the non-axisymmetric behavior of the segmented welding technique. The 2D analyses yield similar hoop residual stresses to the 3D analysis, and closely capture the maximum and minimum ID surface hoop residual stresses from the 3D analysis. The primary difference in ID surface residual stresses between the 2D fixed-fixed and 2D fixed-free constraints cases is the higher tensile axial stresses in the pipe outside of the weld region. The 2D analyses under-predict the maximum axial residual stress compared to the 3D analysis. The 2D ID surface residual stress results tend to bound the averaged 3D results. 2D axisymmetric modeling tends to significantly under-predict weld shrinkage. Axial weld shrinkage from 3D modeling is of the same magnitude as values measured in the laboratory on a prototypic mockup.

scholarly journals The Role of 2D and 3D Echo in Mitral Stenosis

2021 ◽  
Vol 8 (12) ◽  
pp. 171
Author(s):  
Juan Manuel Monteagudo Ruiz ◽  
José Luis Zamorano Gómez
Keyword(s):  
Heart Valve ◽  
Transthoracic Echocardiography ◽  
Mitral Stenosis ◽  
Imaging Modality ◽  
Three Dimensional ◽  
Heart Valve Disease ◽  
Two Dimensional ◽  
3D Echo ◽  
2D And 3D

Mitral stenosis is an important cause of heart valve disease globally. Echocardiography is the main imaging modality used to diagnose and assess the severity and hemodynamic consequences of mitral stenosis as well as valve morphology. Transthoracic echocardiography (TTE) is sufficient for the management of most patients. The focus of this review is the role of current two-dimensional (2D) and three-dimensional (3D) echocardiographic imaging for the evaluation of mitral stenosis.

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