scholarly journals Transcranial direct-current stimulation (tDCS) in the primary motor cortex and its effects on sensorimotor function: a quasi-experimental single-blind sham-controlled trial

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sergio Lerma-Lara ◽  
Marina De Cherade Montbron ◽  
Mathias Guérin ◽  
Ferran Cuenca-Martínez ◽  
Roy La Touche
Keyword(s):  
Effect Size ◽  
Primary Motor Cortex ◽  
Biceps Brachii ◽  
Rectus Femoris ◽  
Thenar Eminence ◽  
Group Differences ◽  
Emg Activity ◽  
Sensorimotor Function ◽  
Tactile Acuity ◽  
Small Effect Size

AbstractThe main aim was to assess the short-term effects of active-tDCS (a-tDCS) in the primary motor cortex (anodal stimulation-M1) on sensorimotor variables. These variables included discriminative sensation through the two-point discrimination (2-PD) test, tactile acuity threshold and pressure pain threshold (PPT), and electromyographic (EMG) activity compared with a sham-tDCS (s-tDCS) in healthy individuals. A total of 100 participants were included. Fifty of the participants received the a-tDCS application of 2 mA for 20 min, whereas the remaining fifty received the s-tDCS. The 2-PD and tactile acuity threshold in thenar eminence of the hand and in the dorsal part of the foot and also, PPT and EMG activity during maximal voluntary contraction in the biceps brachii and rectus femoris were assessed before and after the tDCS application. The a-tDCS intervention was not significantly superior to the s-tDCS in any variable. However, significant within-group pre- and post-intervention differences were found in the a-tDCS, such as the tactile acuity threshold in thenar eminence of the hand, with a small effect size (p = .012, d = 0.20) and in the PPT of the rectus femoris, also with a small effect size (p = .001, d =  − 0.17). Regarding EMG activity, a trend towards greater activity was observed in participants with a-tDCS compared with s-tDCS, which showed a trend towards decreased EMG activity. In fact, although no differences were found between the groups, within-group differences were statistically significant in the biceps brachii pre- and postintervention (p = .023, d =  − 0.16, and p = .002, d = 0.18, respectively), and also in the rectus femoris, only in the a-tDCS, with a small effect size (p = .011, d =  − 0.14). This study showed no significant between-group differences in sensorimotor outcomes. A single session of tDCS in isolation appears to produce immediate effects in healthy participants on sensorimotor function; however, these effects were very small.

scholarly journals Do animated triangles reveal a marked difficulty among autistic people with reading minds?

Autism ◽  
2021 ◽  
pp. 136236132198915
Author(s):  
Alexander C Wilson
Keyword(s):  
Effect Size ◽  
Meta Analysis ◽  
General Information ◽  
Medium Effect ◽  
Group Differences ◽  
Social Challenges ◽  
Autistic People ◽  
The Social ◽  
Small Effect Size ◽  
The Difference

This meta-analysis tested whether autistic people show a marked, isolated difficulty with mentalising when assessed using the Frith -Happé Animations, an advanced test of mentalising (or ‘theory of mind’). Effect sizes were aggregated in multivariate meta-analysis from 33 papers reporting data for over 3000 autistic and non-autistic people. Relative to non-autistic individuals, autistic people underperformed, with a small effect size on the non-mentalising control conditions and a medium effect size on the mentalising condition. This indicates that studies have reliably found mentalising to be an area of challenge for autistic people, although the group differences were not large. It remains to be seen how important mentalising difficulties are in accounting for the social difficulties diagnostic of autism. As autistic people underperformed on the control conditions as well as the mentalising condition, it is likely that group differences on the test are partly due to domain-general information processing differences. Finally, there was evidence of publication bias, suggesting that true effects on the Frith -Happé Animations may be somewhat smaller than reported in the literature. Lay abstract Autistic people are thought to have difficulty with mentalising (our drive to track and understand the minds of other people). Mentalising is often measured by the Frith -Happé Animations task, where individuals need to interpret the interactions of abstract shapes. This review article collated results from over 3000 people to assess how autistic people performed on the task. Analysis showed that autistic people tended to underperform compared to non-autistic people on the task, although the scale of the difference was moderate rather than large. Also, autistic people showed some difficulty with the non-mentalising as well as mentalising aspects of the task. These results raise questions about the scale and specificity of mentalising difficulties in autism. It also remains unclear how well mentalising difficulties account for the social challenges diagnostic of autism.

Lesion in the lateral cerebellum specifically produces overshooting of the toe trajectory in leading forelimb during obstacle avoidance in the rat

2013 ◽  
Vol 110 (7) ◽  
pp. 1511-1524 ◽  
Author(s):  
Sho Aoki ◽  
Yamato Sato ◽  
Dai Yanagihara
Keyword(s):  
Obstacle Avoidance ◽  
Posterior Parietal Cortex ◽  
Primary Motor Cortex ◽  
Biceps Brachii ◽  
Elbow Flexion ◽  
Emg Activity ◽  
Visual Guidance ◽  
The Central Nervous System ◽  
Posterior Parietal

During locomotion, stepping over an obstacle under visual guidance is crucial to continuous safe walking. Studies of the role of the central nervous system in stepping movements have focused on cerebral cortical areas such as the primary motor cortex and posterior parietal cortex. There is speculation that the lateral cerebellum, which has strong anatomical connections with the cerebral cortex, also plays a key role in stepping movements over an obstacle, although this function of the lateral cerebellum has not yet been elucidated. Here we investigated the role of the lateral cerebellum during obstacle avoidance locomotion in rats with a lateral cerebellar lesion. A unilateral lesion in the lateral cerebellum did not affect limb movements during overground locomotion. Importantly, however, the lesioned animals showed overshooting of the toe trajectory specific to the leading forelimb ipsilateral to the lesion when stepping over an obstacle, and the peak toe position, in which the toe is maximally raised during stepping, shifted away from the upper edge of the obstacle. Recordings of EMG activity from elbow flexor and extensor muscles suggested that the overshooting toe trajectory in the ipsilateral leading forelimb possibly resulted from sustained elbow flexion and delayed elbow extension following prolonged activity of the biceps brachii. These results suggest that the lateral cerebellum specifically contributes to generating appropriate toe trajectories in the ipsilateral leading forelimb and to controlling related muscle activities in stepping over an obstacle, especially when accurate control of the distal extremity is achieved under visual guidance.

scholarly journals Comparison of Official and Friendly Matches through Acceleration, Deceleration and Metabolic Power Measures: A Full-Season Study in Professional Soccer Players

2021 ◽  
Vol 18 (11) ◽  
pp. 5980
Author(s):  
Hadi Nobari ◽  
Sara Mahmoudzadeh Khalili ◽  
Rafael Oliveira ◽  
Alfonso Castillo-Rodríguez ◽  
Jorge Pérez-Gómez ◽  
...  
Keyword(s):  
Effect Size ◽  
Total Duration ◽  
Soccer Players ◽  
Metabolic Power ◽  
Professional Soccer ◽  
Premier League ◽  
Small Effect Size ◽  
Moderate Effect Size ◽  
High Effort

Soccer is a popular team sport and highly demanding activity that requires high effort and long-term training plans. The goals of this study were to compare the accelerations, decelerations and metabolic power between official and friendly full matches, between the first and second halves of the matches, and between both halves of official and friendly matches. Twelve professional soccer players (age, 28.6 ± 2.7 years; height, 182.1 ± 8.6 cm; body mass, 75.3 ± 8.2 kg; BMI, 22.6 ± 0.7 kg/m2) participated in this study. A total of 33 official and 10 friendly matches were analyzed from the Iranian Premier League. All matches were monitored using GPSPORTS systems Pty Ltd. The following variables were selected: total duration of the matches, metabolic power, accelerations Zone1 (<2 m·s−2) (AccZ1), accelerations Zone2 (2 to 4 m·s−2) (AccZ2), accelerations Zone3 (>4 m·s−2) (AccZ3), decelerations Zone1 (<−2 m·s−2) (DecZ1), decelerations Zone2 (−2 to −4 m·s−2) (DecZ2) and decelerations Zone3 (>−4 m·s−2) (DecZ3). The major finding was shown in metabolic power, where higher values occurred in friendly matches (p < 0.05 with small effect size). Furthermore, total duration, AccZ3, DecZ1, DecZ2, and DecZ3 were revealed to be higher in official matches, while AccZ1 and AccZ2 were higher in friendly matches. The second half of the official matches revealed higher values for total duration compared to friendly matches (p < 0.05, moderate effect size). In conclusion, this study observed higher values of metabolic power in friendly matches compared to official matches. AccZ3, DecZ1, DecZ2, and DecZ3 were higher in official matches, while AccZ1 and AccZ2 were higher in friendly matches.

scholarly journals Transdiagnostic comparison of visual working memory capacity in bipolar disorder and schizophrenia

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Catherine V. Barnes-Scheufler ◽  
Caroline Passow ◽  
Lara Rösler ◽  
Jutta S. Mayer ◽  
Viola Oertel ◽  
...  
Keyword(s):  
Bipolar Disorder ◽  
Working Memory ◽  
Visual Working Memory ◽  
Effect Size ◽  
Working Memory Capacity ◽  
Memory Capacity ◽  
Medium Effect ◽  
Full Spectrum ◽  
Small Effect Size ◽  
The Difference

Abstract Background Impaired working memory is a core cognitive deficit in both bipolar disorder and schizophrenia. Its study might yield crucial insights into the underpinnings of both disorders on the cognitive and neurophysiological level. Visual working memory capacity is a particularly promising construct for such translational studies. However, it has not yet been investigated across the full spectrum of both disorders. The aim of our study was to compare the degree of reductions of visual working memory capacity in patients with bipolar disorder (PBD) and patients with schizophrenia (PSZ) using a paradigm well established in cognitive neuroscience. Methods 62 PBD, 64 PSZ, and 70 healthy controls (HC) completed a canonical visual change detection task. Participants had to encode the color of four circles and indicate after a short delay whether the color of one of the circles had changed or not. We estimated working memory capacity using Pashler’s K. Results Working memory capacity was significantly reduced in both PBD and PSZ compared to HC. We observed a small effect size (r = .202) for the difference between HC and PBD and a medium effect size (r = .370) for the difference between HC and PSZ. Working memory capacity in PSZ was also significantly reduced compared to PBD with a small effect size (r = .201). Thus, PBD showed an intermediate level of impairment. Conclusions These findings provide evidence for a gradient of reduced working memory capacity in bipolar disorder and schizophrenia, with PSZ showing the strongest degree of impairment. This underscores the importance of disturbed information processing for both bipolar disorder and schizophrenia. Our results are compatible with the cognitive manifestation of a neurodevelopmental gradient affecting bipolar disorder to a lesser degree than schizophrenia. They also highlight the relevance of visual working memory capacity for the development of both behavior- and brain-based transdiagnostic biomarkers.

scholarly journals Electromyographic Analysis of Traditional and Nontraditional Abdominal Exercises: Implications for Rehabilitation and Training

2006 ◽  
Vol 86 (5) ◽  
pp. 656-671 ◽  
Author(s):  
Rafael F Escamilla ◽  
Eric Babb ◽  
Ryan DeWitt ◽  
Patrick Jew ◽  
Peter Kelleher ◽  
...  
Keyword(s):  
Muscle Activity ◽  
Latissimus Dorsi ◽  
Rectus Femoris ◽  
Rectus Abdominis ◽  
Oblique Muscle ◽  
Emg Activity ◽  
Internal Oblique ◽  
Rectus Femoris Muscle ◽  
Femoris Muscle ◽  
Roll Out

Abstract Background and Purpose. Performing nontraditional abdominal exercises with devices such as abdominal straps, the Power Wheel, and the Ab Revolutionizer has been suggested as a way to activate abdominal and extraneous (nonabdominal) musculature as effectively as more traditional abdominal exercises, such as the crunch and bent-knee sit-up. The purpose of this study was to test the effectiveness of traditional and nontraditional abdominal exercises in activating abdominal and extraneous musculature. Subjects. Twenty-one men and women who were healthy and between 23 and 43 years of age were recruited for this study. Methods. Surface electromyography (EMG) was used to assess muscle activity from the upper and lower rectus abdominis, external and internal oblique, rectus femoris, latissimus dorsi, and lumbar paraspinal muscles while each exercise was performed. The EMG data were normalized to maximum voluntary muscle contractions. Differences in muscle activity were assessed by a 1-way, repeated-measures analysis of variance. Results. Upper and lower rectus abdominis, internal oblique, and latissimus dorsi muscle EMG activity were highest for the Power Wheel (pike, knee-up, and roll-out), hanging knee-up with straps, and reverse crunch inclined 30 degrees. External oblique muscle EMG activity was highest for the Power Wheel (pike, knee-up, and roll-out) and hanging knee-up with straps. Rectus femoris muscle EMG activity was highest for the Power Wheel (pike and knee-up), reverse crunch inclined 30 degrees, and bent-knee sit-up. Lumbar paraspinal muscle EMG activity was low and similar among exercises. Discussion and Conclusion. The Power Wheel (pike, knee-up, and roll-out), hanging knee-up with straps, and reverse crunch inclined 30 degrees not only were the most effective exercises in activating abdominal musculature but also were the most effective in activating extraneous musculature. The relatively high rectus femoris muscle activity obtained with the Power Wheel (pike and knee-up), reverse crunch inclined 30 degrees, and bent-knee sit-up may be problematic for some people with low back problems.

One-trial adaptation of movement to changes in load

1996 ◽  
Vol 75 (1) ◽  
pp. 60-74 ◽  
Author(s):  
D. L. Weeks ◽  
M. P. Aubert ◽  
A. G. Feldman ◽  
M. F. Levin
Keyword(s):  
Equilibrium State ◽  
Joint Angle ◽  
Biceps Brachii ◽  
Isometric Force ◽  
Dominant Role ◽  
Load Condition ◽  
Emg Activity ◽  
Positional Error ◽  
Rapid Adaptation ◽  
Load Conditions

1. We analyzed the rapid adaptation of elbow movement to unexpected changes in external load conditions at the elbow joint. The experimental approach was based on the lambda model, which defines control variables (CVs) setting the positional frames of reference for recruitment of flexor and extensor motoneurons. CVs may be specified by the nervous system independently of the current values of output variable such as electromyographic (EMG) activity, muscle torques, and kinematics. The CV R specifies the referent joint angle (R) at which the transition of flexor to extensor activity or vice versa can be observed during changes in the actual joint angle, theta, elicited by an external force. The other CV, the coactivation (C) command, instead of a single transition angle, defines an angular range in which flexor and extensor muscles may be simultaneously active (if C > 0) or silent (if C < 0). Changes in the R command result in shifts in the equilibrium state of the system, a dynamic process leading to EMG modifications resulting in movement or isometric force production if movement is obstructed. Fast movements are likely produced by combining the R command with a positive C command, which provides movement stability and effective energy dissipation, diminishing oscillations at the end of movement. 2. According to the model, changes in the load characteristic (e.g., from a 0 to a springlike load) influence the system's equilibrium state, leading to a positional error. This error may be corrected by a secondary movement produced by additional changes in R and C commands. In subsequent trials, the system may reproduce the CVs specified after correction in the previous trial. This behavior is called the recurrent strategy. It allows the system to adapt to the new load condition in the subsequent trials without corrections (1-trial adaptation). Alternatively, the system may reproduce the CVs specified before correction (invariant strategy). If the movement was perturbed only in a single trial, the invariant strategy allows the system to reach the target in the subsequent trials without corrections. 3. To test the assumption on the dominant role of the recurrent strategy in rapid adaptation of movement to new load conditions, we performed experiments in which subjects (n = 6) used a pivoting manipulandum and made fast 60 degrees movements to a target. After a random number of trials (5-10) with no load, we introduced opposing (experiment 1), assisting (experiment 2), or randomly varied opposing or assisting loads (experiment 3) for 5-10 trials before unexpectedly switching loads again (14-18 switches in total). The opposing or assisting torque was created by position feedback to a torque motor and was a linear function of the displacement of the manipulandum form the initial position (springlike load). Subjects were instructed to correct positional errors as soon as possible to reach the target. The EMG activity of two elbow flexors (biceps brachii and brachioradialis) and two elbow extensors (triceps brachii and anconeus), elbow position, velocity, and torque were recorded. Kinematic and EMG patterns were compared with those obtained in similar experiments in which subjects were instructed not to correct errors. 4. In 94% of the trials in which a change in the load occurred, the primary movement was in error and was followed by a corrective secondary movement. In primary movements, both the phasic and tonic levels of EMG activity as well as the kinematics were load dependent, implicating reflex and intramuscular mechanisms in the adaptation of muscle forces counteracting external loads. These mechanisms, however, were not sufficient to eliminate positional errors. 5. An undershoot error occurred in trials with an opposing load after those with no load or in trials with no load after those with an assisting load. After adaptation to a new load condition, a sudden return to the previous load condition resulted in an error of the oppo

Exploring the Morning Morality Effect in the context of moral utilitarianism: a post-hoc analysis

2021 ◽  
Author(s):  
Bastien Trémolière ◽  
Corentin J Gosling
Keyword(s):  
Effect Size ◽  
Autonomous Vehicles ◽  
Meta Analysis ◽  
Individual Study ◽  
Post Hoc Analysis ◽  
Cognitive Reflection ◽  
Confounding Variables ◽  
Small Effect Size ◽  
Large Heterogeneity ◽  
Post Hoc

Recent research has shown mixed evidence for the morning morality effect (i.e., the observation that individuals are less immoral in the morning than in the afternoon). In the present research, we target the morning morality effect in the context of moral utilitarianism, by reanalyzing observational data previously collected by our lab. These data include different tasks capturing moral utilitarianism (i.e., standard sacrificial dilemmas, an ecological utilitarian scale, and/or dilemmas involving the morality of autonomous vehicles). We report a meta-analysis of 6 studies which showed that participants became less utilitarian as the day goes on, but with a small effect size (r = -0.14) and a large heterogeneity. Exploration of this heterogeneity showed that such a conclusion was statistically significant for classic sacrificial dilemmas only. Notably, even when restricting the analysis to the classic sacrificial dilemmas, a moderate inconsistency remained. Post-hoc analysis of an individual study showed that this small effect did not survive the inclusion of potentially confounding variables, such as psychopathy trait and cognitive reflection. Implications and limitations are discussed.

scholarly journals Motor Cortical Activity During Drawing Movements: Population Representation During Spiral Tracing

1999 ◽  
Vol 82 (5) ◽  
pp. 2693-2704 ◽  
Author(s):  
Daniel W. Moran ◽  
Andrew B. Schwartz
Keyword(s):  
Motor Cortex ◽  
Primary Motor Cortex ◽  
Prediction Interval ◽  
Premotor Cortex ◽  
Cortical Activity ◽  
Simultaneous Action ◽  
Emg Activity ◽  
Radius Of Curvature ◽  
Time Interval ◽  
Population Vector

Monkeys traced spirals on a planar surface as unitary activity was recorded from either premotor or primary motor cortex. Using the population vector algorithm, the hand's trajectory could be accurately visualized with the cortical activity throughout the task. The time interval between this prediction and the corresponding movement varied linearly with the instantaneous radius of curvature; the prediction interval was longer when the path of the finger was more curved (smaller radius). The intervals in the premotor cortex fell into two groups, whereas those in the primary motor cortex formed a single group. This suggests that the change in prediction interval is a property of a single population in primary motor cortex, with the possibility that this outcome is due to the different properties generated by the simultaneous action of separate subpopulations in premotor cortex. Electromyographic (EMG) activity and joint kinematics were also measured in this task. These parameters varied harmonically throughout the task with many of the same characteristics as those of single cortical cells. Neither the lags between joint-angular velocities and hand velocity nor the lags between EMG and hand velocity could explain the changes in prediction interval between cortical activity and hand velocity. The simple spatial and temporal relationship between cortical activity and finger trajectory suggests that the figural aspects of this task are major components of cortical activity.

scholarly journals Effective intracortical microstimulation parameters applied to primary motor cortex for evoking forelimb movements to stable spatial end points

2013 ◽  
Vol 110 (5) ◽  
pp. 1180-1189 ◽  
Author(s):  
Gustaf M. Van Acker ◽  
Sommer L. Amundsen ◽  
William G. Messamore ◽  
Hongyu Y. Zhang ◽  
Carl W. Luchies ◽  
...  
Keyword(s):  
Motor Cortex ◽  
Large Scale ◽  
Primary Motor Cortex ◽  
Emg Activity ◽  
Intracortical Microstimulation ◽  
End Points ◽  
End Point ◽  
Motor Effects ◽  
Forelimb Movements ◽  
Stimulation Of

High-frequency, long-duration intracortical microstimulation (HFLD-ICMS) applied to motor cortex is recognized as a useful and informative method for corticomotor mapping by evoking natural-appearing movements of the limb to consistent stable end-point positions. An important feature of these movements is that stimulation of a specific site in motor cortex evokes movement to the same spatial end point regardless of the starting position of the limb. The goal of this study was to delineate effective stimulus parameters for evoking forelimb movements to stable spatial end points from HFLD-ICMS applied to primary motor cortex (M1) in awake monkeys. We investigated stimulation of M1 as combinations of frequency (30–400 Hz), amplitude (30–200 μA), and duration (0.5–2 s) while concurrently recording electromyographic (EMG) activity from 24 forelimb muscles and movement kinematics with a motion capture system. Our results suggest a range of parameters (80–140 Hz, 80–140 μA, and 1,000-ms train duration) that are effective and safe for evoking forelimb translocation with subsequent stabilization at a spatial end point. The mean time for stimulation to elicit successful movement of the forelimb to a stable spatial end point was 475.8 ± 170.9 ms. Median successful frequency and amplitude were 110 Hz and 110 μA, respectively. Attenuated parameters resulted in inconsistent, truncated, or undetectable movements, while intensified parameters yielded no change to movement end points and increased potential for large-scale physiological spread and adverse focal motor effects. Establishing cortical stimulation parameters yielding consistent forelimb movements to stable spatial end points forms the basis for a systematic and comprehensive mapping of M1 in terms of evoked movements and associated muscle synergies. Additionally, the results increase our understanding of how the central nervous system may encode movement.

Organization of the primate face motor cortex as revealed by intracortical microstimulation and electrophysiological identification of afferent inputs and corticobulbar projections

1988 ◽  
Vol 59 (3) ◽  
pp. 796-818 ◽  
Author(s):  
C. S. Huang ◽  
M. A. Sirisko ◽  
H. Hiraba ◽  
G. M. Murray ◽  
B. J. Sessle
Keyword(s):  
Motor Cortex ◽  
Single Neuron ◽  
Primary Motor Cortex ◽  
General Pattern ◽  
Emg Activity ◽  
Projection Neurons ◽  
Intracortical Microstimulation ◽  
Facial Musculature ◽  
The Face ◽  
Afferent Inputs

1. The technique of intracortical microstimulation (ICMS), supplemented by single-neuron recording, was used to carry out an extensive mapping of the face primary motor cortex. The ICMS study involved a total of 969 microelectrode penetrations carried out in 10 unanesthetized monkeys (Macaca fascicularis). 2. Monitoring of ICMS-evoked movements and associated electromyographic (EMG) activity revealed a general pattern of motor cortical organization. This was characterized by a representation of the facial musculature, which partially enclosed and overlapped the rostral, medial, and caudal borders of the more laterally located cortical regions representing the jaw and tongue musculatures. Responses were evoked at ICMS thresholds as low as 1 microA, and the latency of the suprathreshold EMG responses ranged from 10 to 45 ms. 3. Although contralateral movements predominated, a representation of ipsilateral movements was found, which was much more extensive than previously reported and which was intermingled with the contralateral representations in the anterior face motor cortex. 4. In examining the fine organizational pattern of the representations, we found clear evidence for multiple representation of a particular muscle, thus supporting other investigations of the motor cortex, which indicate that multiple, yet discrete, efferent microzones represent an essential organizational principle of the motor cortex. 5. The close interrelationship of the representations of all three muscle groups, as well as the presence of a considerable ipsilateral representation, may allow for the necessary integration of unilateral or bilateral activities of the numerous face, jaw, and tongue muscles, which is a feature of many of the movement patterns in which these various muscles participate. 6. In six of these same animals, plus an additional two animals, single-neuron recordings were made in the motor and adjacent sensory cortices in the anesthetized state. These neurons were electrophysiologically identified as corticobulbar projection neurons or as nonprojection neurons responsive to superficial or deep orofacial afferent inputs. The rostral, medial, lateral, and caudal borders of the face motor cortex were delineated with greater definition by ICMS and these electrophysiological procedures than by cytoarchitectonic features alone. We noted that there was an approximate fit in area 4 between the extent of projection neurons and field potentials anti-dromically evoked from the brain stem and the extent of positive ICMS sites.(ABSTRACT TRUNCATED AT 400 WORDS)

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