scholarly journals Dissecting industrial fermentations of fine flavour cocoa through metagenomic analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miguel Fernández-Niño ◽  
María Juliana Rodríguez-Cubillos ◽  
Fabio Herrera-Rocha ◽  
Juan Manuel Anzola ◽  
Martha Lucia Cepeda-Hernández ◽  
...  
Keyword(s):  
Metabolic Model ◽  
Starter Cultures ◽  
Microbial Interactions ◽  
Acetobacter Aceti ◽  
Ecological Zones ◽  
Malassezia Globosa ◽  
Global Demand ◽  
On Farm ◽  
Traditional Fermentation ◽  
Agro Ecological Zones

AbstractThe global demand for fine-flavour cocoa has increased worldwide during the last years. Fine-flavour cocoa offers exceptional quality and unique fruity and floral flavour attributes of high demand by the world's elite chocolatiers. Several studies have highlighted the relevance of cocoa fermentation to produce such attributes. Nevertheless, little is known regarding the microbial interactions and biochemistry that lead to the production of these attributes on farms of industrial relevance, where traditional fermentation methods have been pre-standardized and scaled up. In this study, we have used metagenomic approaches to dissect on-farm industrial fermentations of fine-flavour cocoa. Our results revealed the presence of a shared core of nine dominant microorganisms (i.e. Limosilactobacillus fermentum, Saccharomyces cerevisiae, Pestalotiopsis rhododendri, Acetobacter aceti group, Bacillus subtilis group, Weissella ghanensis group, Lactobacillus_uc, Malassezia restricta and Malassezia globosa) between two farms located at completely different agro-ecological zones. Moreover, a community metabolic model was reconstructed and proposed as a tool to further elucidate the interactions among microorganisms and flavour biochemistry. Our work is the first to reveal a core of microorganisms shared among industrial farms, which is an essential step to process engineering aimed to design starter cultures, reducing fermentation times, and controlling the expression of undesirable phenotypes.

scholarly journals On-farm maize storage systems and rodent postharvest losses in six maize growing agro-ecological zones of Kenya

Food Security ◽  
2016 ◽  
Vol 8 (6) ◽  
pp. 1169-1189 ◽  
Author(s):  
Kukom Edoh Ognakossan ◽  
Hippolyte D. Affognon ◽  
Christopher M. Mutungi ◽  
Daniel N. Sila ◽  
Soul-Kifouly G. Midingoyi ◽  
...  
Keyword(s):  
Storage Systems ◽  
Ecological Zones ◽  
Postharvest Losses ◽  
On Farm ◽  
Agro Ecological Zones

scholarly journals YIELD PERFORMANCE AND ADAPTATION OF DESI CHICK PEA VARIETIES IN SELECTED DISTRICTS OF WOLAYTA AND HADIYA ZONES OF SOUTH ETHIOPIA

2016 ◽  
Vol 4 (3) ◽  
pp. 33-41
Author(s):  
Yasin Goa ◽  
Mathewos Ashamo
Keyword(s):  
Grain Yield ◽  
Seed Weight ◽  
Block Design ◽  
Chick Pea ◽  
Ecological Zones ◽  
South Ethiopia ◽  
Randomized Complete Block Design ◽  
Days To Maturity ◽  
On Farm ◽  
Agro Ecological Zones

The experiments were conducted on station and on farm in three districts of Wolayta and Hadiya Zones, south region, Ethiopia, to evaluate the adaptation and yield, assess farmer’s preferences of desi chick pea varieties  to this agro ecological Zones during 2004/05 Meher Season. Data on plant height, hundred seed weight, pod per plant, days to flowering, days to maturity and grain yield were recorded. Five released varieties namely worku, Akaki, Mariye, Dubie, Dz-10-11 and local checks of respective locations were planted on 4.8m2 plots at spacing of 30cm*10cm. The trials were laid in randomized complete block design with three replications. Twelve farmers from three districts at four villages’ three farmers at each village were participated in conducting on farm trials with each farmer as a replicate. There were significant differences among varieties for grain yield and some of traits.  The varieties Akaki and worku were superior yielded overall to the standard and local check across villages ’and on stations. Thus, Akaki and worku out yielded other varieties and had average yields of 1440.95 kg/ha and 1434.75kg/ha at on station and similar trends on farm. The combined statistical analysis and farmers assessments revealed Akaki and worku out yielding other varieties which were also selected by farmers and researchers as the most preferred varieties’. It is therefore recommended that worku and Akaki which had higher yields be promoted for cultivation in the selected districts of south Ethiopia.

scholarly journals On-farm cocoa yields increase with canopy cover of shade trees in two agro-ecological zones in Ghana

2018 ◽  
Vol 11 (5) ◽  
pp. 435-445 ◽  
Author(s):  
Richard Asare ◽  
Bo Markussen ◽  
Rebecca Ashley Asare ◽  
Gilbert Anim-Kwapong ◽  
Anders Ræbild
Keyword(s):  
Canopy Cover ◽  
Shade Trees ◽  
Ecological Zones ◽  
On Farm ◽  
Agro Ecological Zones

scholarly journals Profile of Volatile Compounds of On-Farm Fermented and Dried Cocoa Beans Inoculated with Saccharomyces cerevisiae KY794742 and Pichia kudriavzevii KY794725

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 344
Author(s):  
Gilson Celso Albuquerque Chagas Junior ◽  
Nelson Rosa Ferreira ◽  
Eloisa Helena de Aguiar Andrade ◽  
Lidiane Diniz do Nascimento ◽  
Francilia Campos de Siqueira ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Volatile Compounds ◽  
Multivariate Analyses ◽  
High Capacity ◽  
Starter Cultures ◽  
Cocoa Beans ◽  
Pichia Kudriavzevii ◽  
Fermentative Capacity ◽  
Product Identity ◽  
On Farm

This study aimed to identify the volatile compounds in the fermented and dried cocoa beans conducted with three distinct inoculants of yeast species due to their high fermentative capacity: Saccharomyces cerevisiae, Pichia kudriavzevii, the mixture in equal proportions 1:1 of both species, and a control fermentation (with no inoculum application). Three starter cultures of yeasts, previously isolated and identified in cocoa fermentation in the municipality of Tomé-Açu, Pará state, Brazil. The seeds with pulp were removed manually and placed in wooden boxes for the fermentation process that lasted from 6 to 7 days. On the last day of fermentation, the almonds were packaged properly and placed to dry (36 °C), followed by preparation for the analysis of volatile compounds by GC-MS technique. In addition to the control fermentation, a high capacity for the formation of desirable compounds in chocolate by the inoculants with P. kudriavzevii was observed, which was confirmed through multivariate analyses, classifying these almonds with the highest content of aldehydes, esters, ketones and alcohols and low concentration of off-flavours. We conclude that the addition of mixed culture starter can be an excellent alternative for cocoa producers, suggesting obtaining cocoa beans with desirable characteristics for chocolate production, as well as creating a product identity for the producing region.

scholarly journals Towards Understanding Variability in Droughts in Response to Extreme Climate Conditions over the Different Agro-Ecological Zones of Pakistan

2021 ◽  
Vol 13 (12) ◽  
pp. 6910
Author(s):  
Adil Dilawar ◽  
Baozhang Chen ◽  
Arfan Arshad ◽  
Lifeng Guo ◽  
Muhammad Irfan Ehsan ◽  
...  
Keyword(s):  
Pearson Correlation ◽  
Principal Component ◽  
Mitigation Strategies ◽  
Precipitation Extremes ◽  
Climate Mitigation ◽  
Mean Annual Temperature ◽  
Climate Extreme ◽  
Ecological Zones ◽  
The Mean ◽  
Agro Ecological Zones

Here, we provided a comprehensive analysis of long-term drought and climate extreme patterns in the agro ecological zones (AEZs) of Pakistan during 1980–2019. Drought trends were investigated using the standardized precipitation evapotranspiration index (SPEI) at various timescales (SPEI-1, SPEI-3, SPEI-6, and SPEI-12). The results showed that droughts (seasonal and annual) were more persistent and severe in the southern, southwestern, southeastern, and central parts of the region. Drought exacerbated with slopes of −0.02, −0.07, −0.08, −0.01, and −0.02 per year. Drought prevailed in all AEZs in the spring season. The majority of AEZs in Pakistan’s southern, middle, and southwestern regions had experienced substantial warming. The mean annual temperature minimum (Tmin) increased faster than the mean annual temperature maximum (Tmax) in all zones. Precipitation decreased in the southern, northern, central, and southwestern parts of the region. Principal component analysis (PCA) revealed a robust increase in temperature extremes with a variance of 76% and a decrease in precipitation extremes with a variance of 91% in the region. Temperature and precipitation extremes indices had a strong Pearson correlation with drought events. Higher temperatures resulted in extreme drought (dry conditions), while higher precipitation levels resulted in wetting conditions (no drought) in different AEZs. In most AEZs, drought occurrences were more responsive to precipitation. The current findings are helpful for climate mitigation strategies and specific zonal efforts are needed to alleviate the environmental and societal impacts of drought.

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