Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

AbstractCucumo- and tospoviruses are the most destructive viruses infecting hot pepper (chilli). A diagnostic survey was conducted to assess the prevalence of cucumo and tospoviruses in chilli growing tracts of Tamil Nadu. Infected plants showing mosaic with chlorotic and necrotic rings, veinal necrosis, mosaic mottling, leaf filiformity and malformation were collected. Molecular indexing carried out through reverse transcription polymerase chain reaction (RT-PCR) with coat protein gene specific primer of Cucumber mosaic virus (CMV) and tospovirus degenerate primer corresponding to the L segment (RdRp). Ostensibly, amplifications were observed for both CMV and tospoviruses as sole as well for mixed infections. The sequence analysis indicated that the Capsicum chlorosis virus (CaCV) and Groundnut bud necrosis virus (GBNV) to be involved with CMV in causing combined infections. The co-infection of CMV with CaCV was detected in 10.41% of the symptomatic plant samples and combined infection of CMV with GBNV was recorded in around 6.25% of the symptomatic plants surveyed. The amino acid substitution of Ser129 over conserved Pro129 in coat protein of CMV implies that CMV strain involved in mixed infection as chlorosis inducing strain. Further, the electron microscopy of symptomatic plant samples explicated the presence of isometric particles of CMV and quasi spherical particles of tospoviruses. This is the first molecular evidence for the natural co-existence of chlorosis inducing CMV strain with CaCV and GBNV on hot pepper in India.

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First Report of Cucumber mosaic virus Subgroup II Infecting Lycopersicon esculentum in India

Plant Disease ◽

10.1094/pd-90-1457b ◽

2006 ◽

Vol 90 (11) ◽

pp. 1457-1457 ◽

Cited By ~ 15

Author(s):

N. Sudhakar ◽

D. Nagendra-Prasad ◽

N. Mohan ◽

K. Murugesan

Keyword(s):

Coat Protein ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Tamil Nadu ◽

Indian Subcontinent ◽

Enzyme Linked Immunosorbent Assay ◽

Infected Leaf ◽

Polymorphism Analysis ◽

First Report ◽

Subgroup Ii

During a survey in January 2006 near Salem in Tamil Nadu (south India), Cucumber mosaic virus was observed infecting tomatoes with an incidence of more than 70%. Plants exhibiting severe mosaic, leaf puckering, and stunted growth were collected, and the virus was identified using diagnostic hosts, evaluation of physical properties of the virus, compound enzyme-linked immunosorbent assay (ELISA) (ELISA Lab, Washington State University, Prosser), reverse-transcription polymerase chain reaction (RT-PCR), and restriction fragment length polymorphism analysis (DSMZ, S. Winter, Germany). To determine the specific CMV subgroup, total RNA was extracted from 50 infected leaf samples using the RNeasy plant RNA isolation kit (Qiagen, Hilden, Germany) and tested for the presence of the complete CMV coat protein gene using specific primers as described by Rizos et al. (1). A fragment of the coat protein was amplified and subsequently digested with MspI to reveal a pattern of two fragments (336 and 538 bp), indicating CMV subgroup II. No evidence of mixed infection with CMV subgroup I was obtained when CMV isolates representing subgroups I (PV-0419) and II (PV-0420), available at the DSMZ Plant Virus Collection, were used as controls. Only CMV subgroup I has been found to predominantly infect tomato in the Indian subcontinent, although Verma et al. (2) identified CMV subgroup II infecting Pelargonium spp., an ornamental plant. To our knowledge, this is the first report of CMV subgroup II infecting tomato crops in India. References: (1) H. Rizos et al. J. Gen. Virol. 73:2099, 1992. (2) N. Verma et al. J. Biol. Sci. 31:47, 2006.

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Exogenous application of coat protein-specific dsRNA inhibits cognate cucumber mosaic virus (CMV) of ghost pepper

Journal of Plant Diseases and Protection ◽

10.1007/s41348-021-00558-4 ◽

2022 ◽

Author(s):

Gowtham Kumar Routhu ◽

Munmi Borah ◽

Sundaresha Siddappa ◽

P. D. Nath

Keyword(s):

Coat Protein ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Exogenous Application

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Cucumber mosaic virus. [Distribution map].

Distribution Maps of Plant Diseases ◽

10.1079/dmpd/20066500866 ◽

2002 ◽

Author(s):

Keyword(s):

South Carolina ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Tamil Nadu ◽

Russian Far East ◽

Far East ◽

South Australia ◽

French Polynesia ◽

Peninsular Malaysia ◽

Distribution Map

Abstract A new distribution map is provided for Cucumber mosaic virus Viruses: Bromoviridae: Cucumovirus Hosts: mainly Cucurbitaceae, Solanaceae and Araceae. Information is given on the geographical distribution in EUROPE, Austria, Belarus, Belgium, Bosnia-Herzegovina, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Latvia, Lithuania, Macedonia, Malta, Moldova, Netherlands, Poland, Portugal, Romania, Central Russia Russian Far East, Northern Russia, Southern Russia, Slovakia, Slovenia, Spain, Sweden, Switzerland, UK, Ukraine, Yugoslavia (Fed. Rep.), ASIA, Afghanistan, Bangladesh, China, Anhui, Chongqing, Fujian, Gansu, Guangdong, Guangxi, Guizhou, Hebei, Heilongjiang, Henan, Hong Kong, Hubei, Hunan, Jiangsu, Jiangxi, Jilin, Liaoning, Nei, Menggu, Qinghai, Shaanxi, Shandong, Shanxi, Sichuan, Xinjiang, Yunnan, Zhejiang, Republic of Georgia, India, Andhra Pradesh, Bihar, Delhi, Gujarat, Haryana, Himachal Pradesh, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Orissa, Punjab, Rajasthan, Tamil Nadu, Uttar Pradesh, West Bengal, Indonesia, Java, Iran, Iraq, Israel, Japan, Hokkaido, Honshu, Kyushu, Ryukyu Archipelago, Shikoku, Jordan, Kazakhstan, Korea Republic, Kuwait, Kyrgyzstan, Lebanon, Malaysia, Peninsular Malaysia, Sabah, Sarawak, Nepal, Oman, Pakistan, Philippines, Saudi Arabia, Singapore, Sri Lanka, Syria, Taiwan, Tajikistan, Thailand, Turkey, Uzbekistan, Vietnam, Yemen, AFRICA, Algeria, Cameroon, Cote d'Ivoire, Egypt, Ethiopia, Ghana, Kenya, Mauritius, Morocco, Nigeria, Reunion, Sierra Leone, South Africa, Sudan, Tanzania, Togo, Tunisia, Zambia, Zimbabwe, NORTH AMERICA, Canada, British Columbia, Ontario, Quebec, Mexico, USA, Alabama, Arizona, Arkansas, California, Connecticut, Delaware, Florida, Georgia, Hawaii, Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Maine, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, Missouri, Montana, Nebraska, New Hampshire, New Jersey, New Mexico, New York, North Carolina, Ohio, Oklahoma, Oregon, Pennsylvania, South Carolina, Tennessee, Texas, Utah, Vermont, Virginia, Washington, Wisconsin, Antigua and Barbuda, Barbados, Bermuda, Costa Rica, Cuba, Dominica, Dominican Republic, El Salvador, Guadeloupe, Haiti, Honduras, Jamaica, Martinique, Montserrat, Puerto Rico, St Vincent and Grenadines, Trinidad and Tobago, SOUTH AMERICA, Argentina, Brazil, Ceara, Espirito, Santo, Goias, Maranhao, Minas Gerais, Para, Parana, Piaui, Sao Paulo, Chile, Colombia, French, Guiana, Guyana, Suriname, Venezuela, OCEANIA, Australia, New South Wales, Queensland, South Australia, Tasmania, Victoria, Western, Australia, Cook Islands, Fed. States of Micronesia, Fiji, French, Polynesia, Kiribati, NFW Zealand, Niue, Samoa, Solomon Islands, Tonga, Vanuatu.

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