scholarly journals Medial prefrontal cortex (A32 and A25) projections in the common marmoset: a subcortical anterograde study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jorge Alexander Ríos-Flórez ◽  
Ruthnaldo R. M. Lima ◽  
Paulo Leonardo A. G. Morais ◽  
Helder Henrique Alves de Medeiros ◽  
Jeferson Souza Cavalcante ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Medial Prefrontal Cortex ◽  
Common Marmoset ◽  
Biotinylated Dextran Amine ◽  
Marmoset Monkey ◽  
Subcortical Nuclei ◽  
The Common ◽  
Neuronal Tracers ◽  
Anterograde Tracer ◽  
Biotinylated Dextran

AbstractThis study was aimed at establishing the subcorticals substrates of the cognitive and visceromotor circuits of the A32 and A25 cortices of the medial prefrontal cortex and their projections and interactions with subcortical complexes in the common marmoset monkey (Callithrix jacchus). The study was primarily restricted to the nuclei of the diencephalon and amygdala. The common marmoset is a neotropical primate of the new world, and the absence of telencephalic gyrus favors the mapping of neuronal fibers. The biotinylated dextran amine was employed as an anterograde tracer. There was an evident pattern of rostrocaudal distribution of fibers within the subcortical nuclei, with medial orientation. Considering this distribution, fibers originating from the A25 cortex were found to be more clustered in the diencephalon and amygdala than those originating in the A32 cortex. Most areas of the amygdala received fibers from both cortices. In the diencephalon, all regions received projections from the A32, while the A25 fibers were restricted to the thalamus, hypothalamus, and epithalamus at different densities. Precise deposits of neuronal tracers provided here may significantly contribute to expand our understanding of specific connectivity among the medial prefrontal cortex with limbic regions and diencephalic areas, key elements to the viscerocognitive process.

Colonization of collagen scaffolds by adipocytes derived from mesenchymal stem cells of the common marmoset monkey

2011 ◽  
Vol 411 (2) ◽  
pp. 317-322 ◽  
Author(s):  
Inga Bernemann ◽  
Thomas Mueller ◽  
Rainer Blasczyk ◽  
Birgit Glasmacher ◽  
Nicola Hofmann
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Common Marmoset ◽  
Collagen Scaffolds ◽  
Marmoset Monkey ◽  
The Common

scholarly journals The hormonal induction of cervical remodeling in the common marmoset monkey (Callithrix jacchus)

Reproduction ◽  
2009 ◽  
Vol 137 (3) ◽  
pp. 517-525 ◽  
Author(s):  
Christina Simon ◽  
Almuth Einspanier
Keyword(s):  
Morphological Changes ◽  
Common Marmoset ◽  
Cervical Ripening ◽  
Cervical Tissue ◽  
Ecm Remodeling ◽  
Marmoset Monkey ◽  
Gene And Protein Expression ◽  
The Common

Controversy still exists regarding the involvement of relaxin (RLX) in cervical reorganization throughout parturition in the human, despite its well-known role in facilitating extensive extracellular matrix (ECM) remodeling in diverse organs. Therefore, the aim of the present study was to examine the influence of RLX and estrogen (E2) on the cervical tissue of the common marmoset monkey. Two experimental designs were used: 1)in vivoanalysis of the intracervical diameter under locally applied RLX and 2) ovariectomized (ov) marmosets were treated systemically with either recombinant human (rh) RLX, E2 or rhRLX+E2 to examine their action on the cervix.In vivo-locally applied rhRLX induced a distinct and significant widening of the cervix (before: 4.8±1.1 mm versus after: 5.7±0.9 mm in diameter;P<0.030, MV±s.e.m.). This widening effect was most pronounced in animals without previous pregnancies.In vitroinvestigation of cervical tissue showed significantly increased wet weights after all three hormone treatments (E2: 0.27±0.07 g, RLX: 0.25±0.04 g, E2+RLX: 0.30±0.11 g; allP<0.05; MV±s.e.m.) versus controls (0.10±0.04 g). Furthermore, morphological changes such as loosening of the connective tissue structure and decline in collagen content, an increase in the number of eosinophils, increased the expression of matrix metalloproteinases (MMP1) and MMP2, as well as gene and protein expression of the RLX receptor RXFP1 could be detected in the cervical tissue after all hormone treatments, compared with controls. In summary, RLX has a potent widening effect on the cervix of the common marmoset monkey. Although E2 is not required for this RLX effect, a combined application of E2 and RLX induced the most prominent cervical ripening.

scholarly journals Cross-species comparison of behavioral neurodevelopmental milestones in the common marmoset monkey and human child

2017 ◽  
Vol 59 (7) ◽  
pp. 807-821 ◽  
Author(s):  
Karla K. Ausderau ◽  
Caitlin Dammann ◽  
Kathy McManus ◽  
Mary Schneider ◽  
Marina E. Emborg ◽  
...  
Keyword(s):  
Common Marmoset ◽  
Species Comparison ◽  
Marmoset Monkey ◽  
Human Child ◽  
The Common ◽  
Cross Species Comparison

Co-expression of calretinin and γ-aminobutyric acid in neurons of the entorhinal cortex of the common marmoset monkey

Hippocampus ◽  
2004 ◽  
Vol 14 (5) ◽  
pp. 615-627 ◽  
Author(s):  
Helen H.J. Pothuizen ◽  
Joram Feldon ◽  
Ana L. Jongen-Rêlo
Keyword(s):  
Entorhinal Cortex ◽  
Common Marmoset ◽  
Aminobutyric Acid ◽  
Marmoset Monkey ◽  
The Common

scholarly journals Cryopreservation of cells using defined serum-free cryoprotective agents

2016 ◽  
Vol 2 (1) ◽  
pp. 315-318
Author(s):  
Jan-Cedric Volbers ◽  
Lothar Lauterböck ◽  
Nicola Hofmann ◽  
Birgit Glasmacher
Keyword(s):  
Stress Shielding ◽  
Common Marmoset ◽  
Serum Free ◽  
Poloxamer 188 ◽  
Cryoprotective Agents ◽  
Marmoset Monkey ◽  
Microscopy Analysis ◽  
Fetal Bovine ◽  
The Common ◽  
Shielding Effects

AbstractFor regenerative purposes, there is a high demand for viable and active cells. A big issue is to have enough viable cells available at any given time. One solution is cryopreservation. In this context, DMSO is used as cryoprotective agent (CPA) along with fetal bovine serum for nutrient supply and stress shielding effects. To use these cells for human clinical studies, it is important to eliminate the serum to prevent foreign immune reactions and virus transmittance and DMSO for its toxic effect. In this study a serum free cryopreservation solution and protocol has been established. The combination of methylcellulose and poloxamer 188 provide the basis for the new CPA. Other additves are α-tocopherol, ectoine, prolin and ascorbic acid. The CPAs were examined with 3T3-cells and multipotent stromal cells from the common marmoset monkey (Callithrix jacchus). The cells were preserved with various CPA concentrations, incubation times and different cooling rates. To enable a higher throughput of encouraging conditions a fluorescence microscopy analysis was used. The use of methylcellulose, poloxamer 188 and α-tocopherol enables the reduction of DMSO [up to 2.5% (v/v)] and the elimination of serum without viability losses compared to control.

scholarly journals Monitoring of EAE onset and progression in the common marmoset monkey by sequential high-resolution 3D MRI

2006 ◽  
Vol 19 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Susann Boretius ◽  
Barthel Schmelting ◽  
Takashi Watanabe ◽  
Doron Merkler ◽  
Roland Tammer ◽  
...  
Keyword(s):  
High Resolution ◽  
Common Marmoset ◽  
3D Mri ◽  
Marmoset Monkey ◽  
The Common

scholarly journals The Brain/MINDS 3D digital marmoset brain atlas

2017 ◽  
Author(s):  
Alexander Woodward ◽  
Tsutomu Hashikawa ◽  
Masahide Maeda ◽  
Takaaki Kaneko ◽  
Keigo Hikishima ◽  
...  
Keyword(s):  
White Matter ◽  
Common Marmoset ◽  
Brain Atlas ◽  
File Format ◽  
Modal Data ◽  
Functional Studies ◽  
Marmoset Monkey ◽  
The Common ◽  
The Brain ◽  
Human Primate

AbstractWe present a new 3D digital brain atlas of the non-human primate, common marmoset monkey (Callithrix jacchus), with MRI and coregistered Nissl histology data. To the best of our knowledge this is the first comprehensive digital 3D brain atlas of the common marmoset having normalized multi-modal data, cortical and sub-cortical segmentation, and in a common file format (NIfTI). The atlas can be registered to new data, is useful for connectomics, functional studies, simulation and as a reference.The atlas was based on previously published work but we provide several critical improvements to make this release valuable for researchers. Nissl histology images were processed to remove illumination and shape artifacts and then normalized to the MRI data. Brain region segmentation is provided for both hemispheres. The data is in the NIfTI format making it easy to integrate into neuroscience pipelines, whereas the previous atlas was in an inaccessible file format. We also provide cortical, mid-cortical and white matter boundary segmentations useful for visualization and analysis.

scholarly journals Womb to womb: Maternal litter size and birth weight but not adult characteristics predict early neonatal death of offspring in the common marmoset monkey

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252093
Author(s):  
Julienne N. Rutherford ◽  
Corinna N. Ross ◽  
Toni Ziegler ◽  
Larisa A. Burke ◽  
Alana D. Steffen ◽  
...  
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Pregnancy Outcomes ◽  
Early Life ◽  
Neonatal Death ◽  
Common Marmoset ◽  
Early Neonatal Death ◽  
Marmoset Monkey ◽  
Poisson Models ◽  
The Common

A singular focus on maternal health at the time of a pregnancy leaves much about perinatal mortality unexplained, especially when there is growing evidence for maternal early life effects. Further, lumping stillbirth and early neonatal death into a single category of perinatal mortality may obscure different causes and thus different avenues of screening and prevention. The common marmoset monkey (Callithrix jacchus), a litter-bearing nonhuman primate, is an ideal species in which to study the independent effects of a mother’s early life and adult phenotypes on pregnancy outcomes. We tested two hypotheses in 59 marmoset pregnancies at the Southwest National Primate Research Center and the Barshop Institute for Longevity and Aging Studies. We explored 1) whether pregnancy outcomes were predicted independently by maternal adult weight versus maternal litter size and birth weight, and 2) whether stillbirth and early neonatal death were differentially predicted by maternal variables. No maternal characteristics predicted stillbirth and no maternal adult characteristics predicted early neonatal death. In univariate Poisson models, triplet-born females had a significantly increased rate of early neonatal death (IRR[se] = 3.00[1.29], p = 0.011), while higher birth weight females had a decreased rate (IRR[se] = 0.89[0.05], p = 0.039). In multivariate Poisson models, maternal litter size remained an independent predictor, explaining 13% of the variance in early neonatal death. We found that the later in the first week those neonates died, the more weight they lost. Together these findings suggest that triplet-born and low birth weight females have distinct developmental trajectories underlying greater rates of infant loss, losses that we suggest may be attributable to developmental disruption of infant feeding and carrying. Our findings of early life contributions to adult pregnancy outcomes in the common marmoset disrupt mother-blaming narratives of pregnancy outcomes in humans. These narratives hold that the pregnant person is solely responsible for pregnancy outcomes and the health of their children, independent of socioecological factors, a moralistic framing that has shaped clinical pregnancy management. It is necessary to differentiate temporal trajectories and causes of perinatal loss and view them as embedded in external processes to develop screening, diagnostic, and treatment tools that consider the full arc of a mother’s lived experience, from womb to womb and beyond.

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