A pH-sensitive nanocarrier based on BSA-stabilized graphene-chitosan nanocomposite for sustained and prolonged release of anticancer agents

AbstractSmart nanomaterials with stimuli-responsive behavior are considered as promising platform for various drug delivery applications. Regarding their specific conditions, such as acidic pH, drug carriers to treatment of tumor microenvironment need some criteria to enhance drug delivery efficiency. In this study, for the first time, pH-sensitive BSA-stabilized graphene (BSG)/chitosan nanocomposites were synthesized through electrostatic interactions between the positively charged chitosan nanoparticles and negatively charged BSG and used for Doxorubicin (DOX) encapsulation as a general anticancer drug. Physicochemical characterization of the nanocomposites with different concentrations of BSG (0.5, 2, and 5wt%) showed effective decoration of chitosan nanoparticles on BSG. Comparing DOX release behavior from the nanocomposites and free BSG-chitosan nanoparticles were evaluated at two pHs of 7.4 and 4.5 in 28 days. It was shown that the presence of BSG significantly reduced the burst release observed in chitosan nanoparticles. The nanocomposite of 2wt% BSG was selected as the optimal nanocomposite with a release of 84% in 28 days and with the most uniform release in 24 h. Furthermore, the fitting of release data with four models including zero-order, first-order, Higuchi, and Korsmeyer-Peppas indicated that the addition of BSG changed the release mechanism of the drug, enabling uniform release for the optimal nanocomposite in first 24 h, compared to that for pure chitosan nanoparticles. This behavior was proved using metabolic activity assay of the SKBR-3 breast cancer cell spheroids exposed to DOX release supernatant at different time intervals. It was also demonstrated that DOX released from the nanocomposite had a significant effect on the suppression of cancer cell proliferation at acidic pH.

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Estrone-modified pH-sensitive glycol chitosan nanoparticles for drug delivery in breast cancer

Acta Biomaterialia ◽

10.1016/j.actbio.2018.04.020 ◽

2018 ◽

Vol 73 ◽

pp. 400-411 ◽

Cited By ~ 23

Author(s):

Huan Yang ◽

Cui Tang ◽

Chunhua Yin

Keyword(s):

Breast Cancer ◽

Drug Delivery ◽

Chitosan Nanoparticles ◽

Ph Sensitive ◽

Glycol Chitosan

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Synthesis and Physicochemical Characterization of Amphiphilic Triblock Copolymer Brush Containing pH-Sensitive Linkage for Oral Drug Delivery

Langmuir ◽

10.1021/la301099q ◽

2012 ◽

Vol 28 (21) ◽

pp. 8251-8259 ◽

Cited By ~ 60

Author(s):

You Qiang Yang ◽

Wen Jing Lin ◽

Bin Zhao ◽

Xiu Fang Wen ◽

Xin Dong Guo ◽

...

Keyword(s):

Drug Delivery ◽

Triblock Copolymer ◽

Oral Drug Delivery ◽

Physicochemical Characterization ◽

Ph Sensitive ◽

Oral Drug ◽

Amphiphilic Triblock Copolymer ◽

Copolymer Brush

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pH-Sensitive Nanoaggregates for Site-Specific Drug-Delivery as Well as Cancer Cell Imaging

ACS Omega ◽

10.1021/acsomega.6b00167 ◽

2016 ◽

Vol 1 (5) ◽

pp. 755-764 ◽

Cited By ~ 13

Author(s):

Saikat Mukherjee ◽

Jayasri Das Sarma ◽

Raja Shunmugam

Keyword(s):

Drug Delivery ◽

Cancer Cell ◽

Cell Imaging ◽

Ph Sensitive ◽

Specific Drug ◽

Site Specific ◽

Cancer Cell Imaging

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Novel pH-sensitive and biodegradable micelles for the combined delivery of doxorubicin and conferone to induce apoptosis in MDA-MB-231 breast cancer cell line

RSC Advances ◽

10.1039/d0ra03467c ◽

2020 ◽

Vol 10 (49) ◽

pp. 29228-29246 ◽

Cited By ~ 1

Author(s):

Akram Rahmani ◽

Hassan Zavvar Mousavi ◽

Roya Salehi ◽

Ahmad Bagheri

Keyword(s):

Breast Cancer ◽

Drug Delivery ◽

Cell Line ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cancer Chemotherapy ◽

Breast Cancer Cell Line ◽

Cancer Cell Line ◽

Ph Sensitive ◽

Line P

pH-sensitive micelles are desirable for co-drug delivery in cancer chemotherapy.

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Surface-fluorinated and pH-sensitive carboxymethyl chitosan nanoparticles to overcome biological barriers for improved drug delivery in vivo

Carbohydrate Polymers ◽

10.1016/j.carbpol.2018.12.063 ◽

2019 ◽

Vol 208 ◽

pp. 59-69 ◽

Cited By ~ 16

Author(s):

Xu Cheng ◽

Xiaoli Zeng ◽

Yan Zheng ◽

Xin Wang ◽

Rupei Tang

Keyword(s):

Drug Delivery ◽

Chitosan Nanoparticles ◽

Carboxymethyl Chitosan ◽

Ph Sensitive ◽

Biological Barriers

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Combine Drug Delivery of Thymoquinone-Doxorubicin by Cockle Shellderived pH-sensitive Aragonite CaCO3 Nanoparticles

Nanoscience &Nanotechnology-Asia ◽

10.2174/2210681209666190508122540 ◽

2020 ◽

Vol 10 (4) ◽

pp. 518-533 ◽

Cited By ~ 2

Author(s):

Kehinde M. Ibiyeye ◽

Abu B.Z. Zuki ◽

Norshariza Nurdin ◽

Mokrish Ajat

Keyword(s):

Electron Microscopy ◽

Drug Delivery ◽

Calcium Carbonate ◽

Drug Release ◽

Release Kinetics ◽

Drug Loading ◽

Ph Sensitive ◽

Multiple Drug ◽

Burst Release ◽

Cockle Shell

Background: Cockleshell-derived aragonite calcium carbonate nanoparticles were prepared by the top-down approach for combine delivery of two types of drugs. Objective: The aim of this study was to synthesize and characterize thymoquinone-doxorubicin loaded cockle shell-derived aragonite calcium carbonate nanoparticle. Aragonite calcium carbonate nanoparticles encapsulating thymoquinone and doxorubicin alone were also prepared. Methods: The blank and drug-loaded nanoparticles were characterized by field emission scanning electron microscopy, transmission electron microscopy, Zeta potential, Fourier transformed infrared and X-ray diffraction. Drug delivery properties, in vitro drug release study at pH 7.4, 6 and 4.8, and effect of blank nanoparticles on MCF10A, 3T3, MDA MB231 cells were also analyzed. Results: The blank and drug-loaded nanoparticles were pleomorphic and their sizes varying from 53.65 ± 10.29 nm to 60.49 ± 11.36 nm with an overall negative charge. The entrapment efficiency of thymoquinone and doxorubicin were 41.6 and 95.8, respectively. The FTIR showed little alteration after loading thymoquinone and doxorubicin while XRD patterns revealed no changes in the crystallizations of nanoparticles after drug loading. The drug release kinetics of doxorubicin and thymoquinone from the nanoparticles showed a continuous and gradual release after an initial burst release was observed. At pH 4.8, about 100% of drug release was noticed, 70% at pH 6 while only 50% at pH 7.4. The cell viability was 80% at a concentration of 1000 ug/ml of blank nanoparticle. Conclusion: The cockle shell-derived pH sensitive aragonite calcium carbonate nanoparticle provides an effective and simple means of multiple drug delivery and function as a platform for pH controlled release of loaded therapeutic agents.

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pH Sensitive Hydrogels in Drug Delivery: Brief History, Properties, Swelling, and Release Mechanism, Material Selection and Applications

Polymers ◽

10.3390/polym9040137 ◽

2017 ◽

Vol 9 (12) ◽

pp. 137 ◽

Cited By ~ 129

Author(s):

Muhammad Rizwan ◽

Rosiyah Yahya ◽

Aziz Hassan ◽

Muhammad Yar ◽

Ahmad Azzahari ◽

...

Keyword(s):

Drug Delivery ◽

Material Selection ◽

Ph Sensitive ◽

Release Mechanism

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Cellulose/Exosome Nanocarrier for Improved Prolonged Release of 5-Fluorouracil: Preparation, Characterization, Drug Release Behavior and Cytotoxicity

10.21203/rs.3.rs-1102085/v1 ◽

2021 ◽

Author(s):

Maryam Saeidifar ◽

Mobina Seyedahmadi ◽

Jafar Javadpour ◽

Hamid Reza Rezaei

Keyword(s):

Drug Delivery ◽

Controlled Release ◽

Cancer Cell Line ◽

Human Colon ◽

Fickian Diffusion ◽

Release Mechanism ◽

Prolonged Release ◽

Release Behavior ◽

Scanning Calorimetry ◽

Human Colon Cancer

Abstract Designing of nanoparticle drug delivery systems and improving the efficacy of anticancer drugs are a great deal of effort in the recent years. In this study, a novel biocompatible nanocarrier based on bacterial cellulose (BC) in presence of exosome (Exo) was prepared to controlled release of 5-fluorouracil (5-FU), (5-FU.Exo@BC). The physicochemical properties of 5-FU.Exo@BC was characterized using field emission scanning electron microscopy (FESEM), Differential Scanning Calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), and X-ray Diffraction (XRD) techniques that confirmed the successful preparation of 5-FU.Exo@BC. The release behavior of 5-FU.Exo@BC compared to 5-FU and 5-FU@BC demonstrated a significant sustained release during 162 h. The release mechanism of the above three systems followed Korsmeyer-peppas with non-Fickian diffusion for 5-FU@BC and 5-FU.Exo@BC. In addition, the viability of HT-29 cells (human colon cancer cell line), towards BC, 5-FU@BC and 5-FU.Exo@BC indicated the promising efficacy of 5-FU into 5-FU.Exo@BC. Subsequently, the prepared bio-nanocomposite could be proposed as a potential drug delivery system with effective controlled-release function.

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