scholarly journals Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ru-Fang Siao ◽  
Chia-Hsuan Lin ◽  
Li-Hsuan Chen ◽  
Liang-Chun Wang
Keyword(s):  
Tissue Culture ◽  
Aeromonas Hydrophila ◽  
Ex Vivo ◽  
Infection Model ◽  
Culture Model ◽  
Skin Response ◽  
Tissue Culture Model ◽  
Striped Catfish ◽  
Aquatic Pathogen

AbstractTeleost fish skin serves as the first line of defense against pathogens. The interaction between pathogen and host skin determines the infection outcome. However, the mechanism(s) that modulate infection remain largely unknown. A proper tissue culture model that is easier to handle but can quantitatively and qualitatively monitor infection progress may shed some lights. Here, we use striped catfish (Pangasius hypophthalmus) to establish an ex vivo skin explant tissue culture model to explore host pathogen interactions. The skin explant model resembles in vivo skin in tissue morphology, integrity, and immune functionality. Inoculation of aquatic pathogen Aeromonas hydrophila in this model induces epidermal exfoliation along with epithelial cell dissociation and inflammation. We conclude that this ex vivo skin explant model could serve as a teleost skin infection model for monitoring pathogenesis under various infection conditions. The model can also potentially be translated into a platform to study prevention and treatment of aquatic infection on the skin in aquaculture applications.

scholarly journals An ex vivo Tissue Culture Model for the Assessment of Individualized Drug Responses in Prostate and Bladder Cancer

2018 ◽  
Vol 8 ◽  
Author(s):  
Arjanneke F. van de Merbel ◽  
Geertje van der Horst ◽  
Maaike H. van der Mark ◽  
Janneke I. M. van Uhm ◽  
Erik J. van Gennep ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Bladder Cancer ◽  
Ex Vivo ◽  
Drug Responses ◽  
Culture Model ◽  
Tissue Culture Model ◽  
Ex Vivo Tissue

Abstract 158: Ultrasound-Induced Inhibition and Modulation of Neonatal Ventricular Cardiomyocyte Depolarization

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Natasha Mehta ◽  
J Yasha Kresh ◽  
Steven P Kutalek ◽  
Peter A Lewin ◽  
Andrew R Kohut
Keyword(s):  
Tissue Culture ◽  
Acoustic Energy ◽  
Neonatal Rat ◽  
Calcium Handling ◽  
P Value ◽  
Function Generator ◽  
Ultrasound Exposure ◽  
Culture Model ◽  
Tissue Culture Model

Background: Ultrasound can interact with tissue through either thermal or non-thermal physical mechanisms. Radiation force has been shown to stimulate cardiac and neural tissue in vivo. Ultrasound might hold clinical potential as a noninvasive therapeutic tool via specific bioeffects on cardiomyocytes. This study aims to assess the effect of ultrasound on cardiomyocyte depolarization in a tissue culture model. Methods: Cardiomyocytes were isolated from neonatal rat ventricular tissue and plated directly on microelectrode arrays to record depolarization patterns. A custom 2.5 MHz unfocused ultrasound transducer was directed at the cardiomyocytes in a tissue culture model. A function generator, with an amplified signal +50 dB, delivered acoustic energy at variable settings of 0.1, 0.3, 0.5 and 1.0 Vpp, pulse durations of 2, 5 and 10 ms, and burst periods of 100, 250 and 300 ms. Five trials were conducted at each setting (36 total trials) with 30s of continuous ultrasound exposure followed by an off interval of 1 minute. Results: The R-R interval durations (ID) were measured throughout the recording period. Prior to ultrasound delivery, the IDs were highly irregular, ID range = 0.3-2.7 s. As ultrasound was delivered in an asynchronous manner, using 0.1 and 0.3 Vpp and PD = 2 and 5 ms, there was suppression/inhibition of cellular depolarization for the first 5-10 s. Then 10-15 s after the start of ultrasound delivery, the depolarization rate increased and demonstrated less R-R interval variability (ID=0.88-1.03 s, P value<0.05), even after the ultrasound exposure. Conclusion: Ultrasound can inhibit and modify the frequency of spontaneous electrical depolarizations of neonatal ventricular cardiomyocytes in a tissue culture model. Our observations could be due to conditioning via stretch and compression-mediated mechanosensitive pathways, by modifying intracellular calcium handling or altering cell signaling.

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

10.3791/59467 ◽  
2019 ◽  
Author(s):  
Christian S. Thudium ◽  
Amalie Engstrom ◽  
Solveig S. Groen ◽  
Morten A. Karsdal ◽  
Anne-Christine Bay-Jensen
Keyword(s):  
Tissue Culture ◽  
Ex Vivo ◽  
Culture Model ◽  
Tissue Culture Model ◽  
Ex Vivo Tissue

scholarly journals HySyn: A genetically-encoded synthetic synapse to rewire neural circuits in vivo

2020 ◽  
Author(s):  
Josh D. Hawk ◽  
Daniel A. Colón-Ramos
Keyword(s):  
Tissue Culture ◽  
Caenorhabditis Elegans ◽  
Neural Circuits ◽  
De Novo ◽  
Neural Connectivity ◽  
Neuronal Tissue ◽  
Culture Model ◽  
Tissue Culture Model ◽  
Targeted Expression

Here we introduce HySyn, a system designed to rewire neural connectivity in vivo by reconstituting a functional heterologous synapse. We demonstrate that genetically targeted expression of the two HySyn components, a Hydra-derived neuropeptide and its receptor, creates de novo neuromodulatory transmission in a mammalian neuronal tissue culture model and rewires a behavioral circuit in vivo in the nematode Caenorhabditis elegans. HySyn can interface with existing optogenetic, chemogenetic and pharmacological approaches to functionally probe synaptic transmission, dissect neuropeptide signaling, or modulate specific neural circuits.

scholarly journals Establish a teleost skin explant model for studying the fish-pathogen interaction

2021 ◽  
Author(s):  
Ru-Fang Siao ◽  
Chia-Hsuan Lin ◽  
Liang-Chun Wang
Keyword(s):  
Teleost Fish ◽  
Ex Vivo ◽  
Mucous Cell ◽  
Cell Junction ◽  
Infection Model ◽  
Epithelial Polarity ◽  
Tissue Model ◽  
Infection Mechanisms ◽  
Striped Catfish

Abstract Teleost fish skin serves as the first line of defense against pathogens. The interaction between pathogen and host skin determines the infection outcome. However, the interaction and infection mechanisms remain largely unknown due to the lack of a proper tissue model that can quantitatively and qualitatively monitor infection progress. Here, we use striped catfish (Pangasius hypophthalmus) to establish an ex vivo skin explant tissue model that has not been explored in teleost fish before. The skin explant model, cultured by creating epithelial polarity, resembles the in vivo skin in tissue morphology, integrity, and mucous cell and immune functionality. Inoculation of Aeromonas hydrophila in this model induces epidermal exfoliation along with epithelial cell junction disassembly and inflammation. We therefore concluded that this ex vivo skin explant model could serve as a teleost skin infection model for monitoring pathogenesis under various infection conditions. The model can also potentially be translated into a platform to study prevention and treatment for aquatic infection on mucosal surface in aquaculture applications.

scholarly journals A genetically encoded tool for reconstituting synthetic modulatory neurotransmission and reconnect neural circuits in vivo

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Josh D. Hawk ◽  
Elias M. Wisdom ◽  
Titas Sengupta ◽  
Zane D. Kashlan ◽  
Daniel A. Colón-Ramos
Keyword(s):  
Tissue Culture ◽  
Caenorhabditis Elegans ◽  
Synaptic Transmission ◽  
Neural Circuits ◽  
De Novo ◽  
Neuronal Tissue ◽  
Culture Model ◽  
Tissue Culture Model ◽  
Targeted Expression

AbstractChemogenetic and optogenetic tools have transformed the field of neuroscience by facilitating the examination and manipulation of existing circuits. Yet, the field lacks tools that enable rational rewiring of circuits via the creation or modification of synaptic relationships. Here we report the development of HySyn, a system designed to reconnect neural circuits in vivo by reconstituting synthetic modulatory neurotransmission. We demonstrate that genetically targeted expression of the two HySyn components, a Hydra-derived neuropeptide and its receptor, creates de novo neuromodulatory transmission in a mammalian neuronal tissue culture model and functionally rewires a behavioral circuit in vivo in the nematode Caenorhabditis elegans. HySyn can interface with existing optogenetic, chemogenetic and pharmacological approaches to functionally probe synaptic transmission, dissect neuropeptide signaling, or achieve targeted modulation of specific neural circuits and behaviors.

Sign in / Sign up

Export Citation Format

Share Document