scholarly journals Oxytocinergic cells of the posterior hypothalamic paraventricular nucleus participate in the food entrained clock

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mario Caba ◽  
Enrique Meza ◽  
Carolina Escobar ◽  
Angeles Jiménez ◽  
Mario Daniel Caba-Flores ◽  
...  
Keyword(s):  
Food Intake ◽  
Hypothalamic Nucleus ◽  
Restricted Feeding ◽  
Adult Rats ◽  
Peripheral Oscillators ◽  
Food Anticipatory Activity ◽  
Ad Libitum ◽  
Anticipatory Activity ◽  
Oxytocinergic Cells

AbstractThe mechanisms underlying food anticipatory activity are still poorly understood. Here we explored the role of oxytocin (OT) and the protein c-Fos in the supraoptic nucleus (SON), medial (PVNm) and posterior (PVNp) regions of the paraventricular hypothalamic nucleus. Adult rats were assigned to one of four groups: scheduled restricted feeding (RF), ad libitum (AL), fasting after restricted feeding (RF-F), to explore the possible persistence of oscillations, or ad libitum fasted (AL-F). In the SON and in the PVNm, OT cells were c-Fos positive after food intake; in contrast, OT cells in the PVNp showed c-Fos activation in anticipation to food access, which persisted in RF-F subjects. We conclude that OT and non-OT cells of the SON and PVNm may play a role as recipients of the entraining signal provided by food intake, whereas those of the PVNp which contain motor preautonomic cells that project to peripheral organs, may be involved in the hormonal and metabolic anticipatory changes in preparation for food presentation and thus, may be part of a link between central and peripheral oscillators. In addition, due to their persistent activation they may participate in the neuronal network for the clock mechanism that leads to food entrainment.

scholarly journals Oxytocinergic Cells of the Posterior Hypothalamic Paraventricular Nucleus Participate in the Food Entrained Clock

2021 ◽  
Author(s):  
Mario Caba ◽  
Enrique Meza ◽  
Carolina Escobar ◽  
Angeles Jiménez ◽  
Mario Daniel Caba-Flores ◽  
...  
Keyword(s):  
Food Intake ◽  
Hypothalamic Nucleus ◽  
Restricted Feeding ◽  
Adult Rats ◽  
Peripheral Oscillators ◽  
Food Anticipatory Activity ◽  
Ad Libitum ◽  
Anticipatory Activity ◽  
Oxytocinergic Cells

Abstract The mechanisms underlying food anticipatory activity is still not well understood. Here we explored the role of oxytocin (OT) and the protein c-Fos in the supraoptic nucleus (SON) and in the medial (PVNm) and posterior (PVNp) regions of the paraventricular hypothalamic nucleus. Adult rats were assigned to one of four groups: scheduled restricted feeding (RF), Ad libitum (AL), fasting after restricted feeding (RF-F), to explore the possible persistence of oscillations, or Ad libitum fasted (AL-F). In the SON and in the PVNm, OT cells were c-Fos positive after food intake; contrasting, OT cells in the PVNp showed c-Fos activation in anticipation to food access, which persisted in RF-F subjects. We conclude that OT cells of the SON and PVNm may play a role as recipients of the entraining signal provided by food intake, whereas those of the PVNp which contain motor preautonomic cells that project to peripheral organs, may be involved in the hormonal and metabolic anticipatory changes in preparation for food presentation and thus, may be part of a link between central and peripheral oscillators. In addition, due to their persistent activation they may participate in the neuronal network for the clock mechanism that leads to food entrainment.

scholarly journals Leptin-sensitive neurons in the arcuate nucleus integrate activity and temperature circadian rhythms and anticipatory responses to food restriction

2013 ◽  
Vol 305 (8) ◽  
pp. R949-R960 ◽  
Author(s):  
Michael F. Wiater ◽  
Ai-Jun Li ◽  
Thu T. Dinh ◽  
Heiko T. Jansen ◽  
Sue Ritter
Keyword(s):  
Circadian Rhythms ◽  
Food Restriction ◽  
Arcuate Nucleus ◽  
Restricted Feeding ◽  
Food Anticipatory Activity ◽  
Static Phase ◽  
Integrate Activity ◽  
Arcuate Nuclei ◽  
Anticipatory Activity

Previously, we investigated the role of neuropeptide Y and leptin-sensitive networks in the mediobasal hypothalamus in sleep and feeding and found profound homeostatic and circadian deficits with an intact suprachiasmatic nucleus. We propose that the arcuate nuclei (Arc) are required for the integration of homeostatic circadian systems, including temperature and activity. We tested this hypothesis using saporin toxin conjugated to leptin (Lep-SAP) injected into Arc in rats. Lep-SAP rats became obese and hyperphagic and progressed through a dynamic phase to a static phase of growth. Circadian rhythms were examined over 49 days during the static phase. Rats were maintained on a 12:12-h light-dark (LD) schedule for 13 days and, thereafter, maintained in continuous dark (DD). After the first 13 days of DD, food was restricted to 4 h/day for 10 days. We found that the activity of Lep-SAP rats was arrhythmic in DD, but that food anticipatory activity was, nevertheless, entrainable to the restricted feeding schedule, and the entrained rhythm persisted during the subsequent 3-day fast in DD. Thus, for activity, the circuitry for the light-entrainable oscillator, but not for the food-entrainable oscillator, was disabled by the Arc lesion. In contrast, temperature remained rhythmic in DD in the Lep-SAP rats and did not entrain to restricted feeding. We conclude that the leptin-sensitive network that includes the Arc is required for entrainment of activity by photic cues and entrainment of temperature by food, but is not required for entrainment of activity by food or temperature by photic cues.

scholarly journals The Dorsomedial Hypothalamic Nucleus Is Not Necessary for the Expression of Circadian Food-Anticipatory Activity in Rats

2007 ◽  
Vol 22 (6) ◽  
pp. 467-478 ◽  
Author(s):  
Glenn J. Landry ◽  
Glenn R. Yamakawa ◽  
Ian C. Webb ◽  
Rhiannon J. Mear ◽  
Ralph E. Mistlberger
Keyword(s):  
Hypothalamic Nucleus ◽  
Food Anticipatory Activity ◽  
Dorsomedial Hypothalamic Nucleus ◽  
Anticipatory Activity

Daily gain, food intake and food efficiency in pigs during the growing period

1990 ◽  
Vol 50 (2) ◽  
pp. 353-364 ◽  
Author(s):  
E. Kanis ◽  
W. J. Koops
Keyword(s):  
Food Intake ◽  
Live Weight ◽  
Restricted Feeding ◽  
Growing Period ◽  
Food Efficiency ◽  
Daily Food ◽  
Ad Libitum ◽  
The Difference ◽  
Predicted Values ◽  
Daily Gain

ABSTRACTThe non-linear model y = ae(−hW−c/W) was fitted to weekly calculated daily gain (DG), daily food intake (FI) and food efficiency (FE) of 653 barrows and gilts fed ad libitum or restrictedly during a growing period from 27 to 108 kg live weight. Where y was DG, FI or FE, W was live weight and a, b and c were parameters. The model fitted well to the expected course of the traits, with an accuracy similar to that of quadratic polynomials. Parameters for one trait could simply be derived from the parameters for the other two traits. For each trait, four basic patterns were distinguished, depending on the signs of b and c. Curves with a maximum (b > 0 and c > 0) occurred most frequently. In cases of curves with a maximum, the model could be reparameterized to a model with parameters having a simple biological meaning.Coefficients of determination in barrows averaged 0·29 for DG, 0·88 for FI and 0·45 for FE, whereas these values were somewhat lower in gilts. With ad libitum feeding, a DG curve with a maximum was fitted in proportionately 0·83 of the barrows and 0·61 of the gilts. The maximum DG was on average at live weights of 64 kg for barrows and 77 kg for gilts. A maximum in the FI curve was predicted in proportionately 0·60 of the barrows and 0·39 of the gilts. Curves for DG and FI in gilts were less curvilinear than in barrows. FE curves, with ad libitum feeding, had a maximum in proportionately 0·59 of the barrows and 0·52 of the gilts. This predicted maximum FE was, on average, before the start of the growing period. Gilts had a higher FE than barrows from 35 kg body weight onwards, and the difference increased with increasing live weight. Differences in FE between ad libitum and restricted feeding were small, with a tendency for animals fed at a restricted feeding level to be more efficient at the end of the growing period.Average FE curves and individual FI or DG curves were used for indirect prediction of individual DG or FI curves, respectively. The correlation between directly and indirectly predicted values of DG and FI at live weights of 30, 65 or 100 kg was about 0·7 in ad libitum fed barrows and gilts, and greater than 0·8 in pigs fed at a restricted level. This indicates that the model is suitable to predict and control the course of individual daily gain by influencing the course of food intake.

scholarly journals Type 1 dopamine receptor (D1R)-independent circadian food anticipatory activity in mice

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0242897
Author(s):  
Dina R. Assali ◽  
Michael Sidikpramana ◽  
Andrew P. Villa ◽  
Jeffrey Falkenstein ◽  
Andrew D. Steele
Keyword(s):  
Dorsal Striatum ◽  
D1 Receptor ◽  
Conditional Deletion ◽  
Food Anticipatory Activity ◽  
Significant Impairment ◽  
Receptor Pharmacology ◽  
Cell Type Specific ◽  
Anticipatory Activity

Circadian rhythms are entrained by light and influenced by non-photic stimuli, such as feeding. The activity preceding scheduled mealtimes, food anticipatory activity (FAA), is elicited in rodents fed a limited amount at scheduled times. FAA is thought to be the output of an unidentified food entrained oscillator. Previous studies, using gene deletion and receptor pharmacology, implicated dopamine type receptor 1 (D1R) signaling in the dorsal striatum as necessary for FAA in mice. To further understand the role of D1R in promoting FAA, we utilized the Cre-lox system to create cell type-specific deletions of D1R, conditionally deleting D1R in GABA neurons using Vgat-ires-Cre line. This conditional deletion mutant had attenuated FAA, but the amount was higher than expected based on prior results using a constitutive knockout of D1R, D1R KODrago. This result prompted us to re-test the original D1R KODrago line, which expressed less FAA than controls, but only moderately so. To determine if genetic drift had diminished the effect of D1R deletion on FAA, we re-established the D1R KODrago knockout line from cryopreserved samples. The reestablished D1R KODrago-cryo had a clear impairment of FAA compared to controls, but still developed increased activity preceding mealtime across the 4 weeks of timed feeding. Finally, we tested a different deletion allele of D1R created by the Knockout Mouse Project. This line of D1R KOKOMP mice had a significant impairment in the acquisition of FAA, but eventually reached similar levels of premeal activity compared to controls after 4 weeks of timed feeding. Taken together, our results suggest that D1R signaling promotes FAA, but other dopamine receptors likely contribute to FAA given that mice lacking the D1 receptor still retain some FAA.

Role of hormones in glucose-6-phosphate dehydrogenase adaptation of rat liver

1962 ◽  
Vol 202 (3) ◽  
pp. 401-406 ◽  
Author(s):  
Helen M. Tepperman ◽  
Jay Tepperman
Keyword(s):  
Food Intake ◽  
Hormone Treatment ◽  
Additional Increase ◽  
Phosphogluconate Dehydrogenase ◽  
Hypophysectomized Rats ◽  
Ad Libitum ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Determining Factor ◽  
Rule Out

The hepatic enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase increase markedly in activity on refeeding after a 48-hr fast in the intact rat, but fail to do so in the hypophysectomized animal. Treatment with somatotrophin, cortisone, and thyroid hormone individually and in pairs failed to restore this adaptation to refeeding, but a combination of the three repaired the defect. Similar observations were made on increases in these enzymes in response to fructose feeding in ad libitum-fed hypophysectomized rats. Triple hormone-treated animals in which the enzyme increase in response to fructose feeding was restored were the only ones which gained weight. To rule out possible effects of variations in food intake as a determining factor in the response, hypophysectomized rats fed measured amounts of a starch-fructose diet by stomach tube were compared with suitable controls. A striking increase in enzyme activity occurred in these animals even when no hormone treatment was given, and no additional increase could be induced by treating with the three hormones. It is concluded that the liver cell of the hypophysectomized rat has an autonomous capacity to modify its enzyme profile selectively without the direction or support of hormones of pituitary origin. These hormones may exert their effects in ad libitum-fed animals by influencing food intake and intestinal hexose absorption rates.

Selection for lean growth in pigs with a restricted feeding regime

1994 ◽  
Vol 1994 ◽  
pp. 12-12
Author(s):  
M.K. Curran ◽  
N.D. Cameron ◽  
J.C. Kerr
Keyword(s):  
Growth Rate ◽  
Food Intake ◽  
Restricted Feeding ◽  
Correlated Responses ◽  
Large White ◽  
Daily Food Intake ◽  
Feeding Regime ◽  
Lean Growth ◽  
Daily Food ◽  
Ad Libitum

Divergent selection lines for lean growth on a restricted feeding regime, in Large White and Landrace pigs, were established to complement the lean growth selection lines on ad-libitum feeding. This study estimated the direct and correlated responses after four generations of selection and the corresponding genetic and phenotypic parameters.The selection objective for lean growth on restricted or scale (LGS) feeding was to obtain equal correlated responses in growth rate and carcass lean content, measured in phenotypic s.d. The selection criterion included measurements of growth rate and ultrasonic backfat depth.Large White (LW) and Landrace (LR) boars and gilts were purchased from eight British nucleus herds and boars from national artificial insemination centres in 1982. Homozygous or heterozygous halothane positive pigs were not included in the experiment. The base populations consisted of 31 LW and 19 LR sires and 57 LW and 67 LR dams. Within each population, there were high and low selection lines with a control line, each consisting of 10 boars and 20 gilts, with a generation interval of 13.5 and 12 months for LW and LR pigs. Animals were performance tested in individual pens from 30±3 kg for a period of 84 days and fed a high energy (13.8 MJ DE/kg DM) and high protein (210 g/kg DM crude protein) pelleted ration. Daily food intake was equal to 0.75 g/g of the daily food intake for ad-libitum fed pigs and the total food intake was 134 kg for LW pigs and 150 kg for LR pigs. On average, 3 boars and 3 gilts were tested per litter. The total number of pigs tested per line and average inbreeding coefficients at generation four, by population are given below.

scholarly journals Central Nesfatin-1 Reduces Dark-Phase Food Intake and Gastric Emptying in Rats: Differential Role of Corticotropin-Releasing Factor2 Receptor

Endocrinology ◽  
2009 ◽  
Vol 150 (11) ◽  
pp. 4911-4919 ◽  
Author(s):  
Andreas Stengel ◽  
Miriam Goebel ◽  
Lixin Wang ◽  
Jean Rivier ◽  
Peter Kobelt ◽  
...  
Keyword(s):  
Gastric Emptying ◽  
Food Intake ◽  
Nucleus Tractus Solitarius ◽  
Hypothalamic Nucleus ◽  
Reduce Food Intake ◽  
Dark Phase ◽  
Crf2 Receptor ◽  
Brain Ventricle ◽  
Dose Dependent

Nesfatin-1, derived from nucleobindin2, is expressed in the hypothalamus and reported in one study to reduce food intake (FI) in rats. To characterize the central anorexigenic action of nesfatin-1 and whether gastric emptying (GE) is altered, we injected nesfatin-1 into the lateral brain ventricle (intracerebroventricular, icv) or fourth ventricle (4v) in chronically cannulated rats or into the cisterna magna (intracisternal, ic) under short anesthesia and compared with ip injection. Nesfatin-1 (0.05 μg/rat, icv) decreased 2–3 h and 3–6 h dark-phase FI by 87 and 45%, respectively, whereas ip administration (2 μg/rat) had no effect. The corticotropin-releasing factor (CRF)1/CRF2 antagonist astressin-B or the CRF2 antagonist astressin2-B abolished icv nesfatin-1’s anorexigenic action, whereas an astressin2-B analog, devoid of CRF-receptor binding affinity, did not. Nesfatin-1 icv induced a dose-dependent reduction of GE by 26 and 43% that was not modified by icv astressin2-B. Nesfatin-1 into the 4v (0.05 μg/rat) or ic (0.5 μg/rat) decreased cumulative dark-phase FI by 29 and 60% at 1 h and by 41 and 37% between 3 and 5 h, respectively. This effect was neither altered by ic astressin2-B nor associated with changes in GE. Cholecystokinin (ip) induced Fos expression in 43% of nesfatin-1 neurons in the paraventricular hypothalamic nucleus and 24% of those in the nucleus tractus solitarius. These data indicate that nesfatin-1 acts centrally to reduce dark phase FI through CRF2-receptor-dependent pathways after forebrain injection and CRF2-receptor-independent pathways after hindbrain injection. Activation of nesfatin-1 neurons by cholecystokinin at sites regulating food intake may suggest a role in gut peptide satiation effect.

scholarly journals Circadian rhythms of gastrointestinal function are regulated by both central and peripheral oscillators

2012 ◽  
Vol 303 (4) ◽  
pp. G461-G473 ◽  
Author(s):  
Jaclyn N. Malloy ◽  
Jiffin K. Paulose ◽  
Ye Li ◽  
Vincent M. Cassone
Keyword(s):  
Circadian Rhythms ◽  
Ex Vivo ◽  
Circadian Clocks ◽  
Constant Darkness ◽  
Restricted Feeding ◽  
Chemical Sympathectomy ◽  
Surgical Ablation ◽  
Peripheral Oscillators ◽  
Ad Libitum ◽  
6 Hydroxydopamine

Circadian clocks are responsible for daily rhythms in a wide array of processes, including gastrointestinal (GI) function. These are vital for normal digestive rhythms and overall health. Previous studies demonstrated circadian clocks within the cells of GI tissue. The present study examines the roles played by the suprachiasmatic nuclei (SCN), master circadian pacemaker for overt circadian rhythms, and the sympathetic nervous system in regulation of circadian GI rhythms in the mouse Mus musculus . Surgical ablation of the SCN abolishes circadian locomotor, feeding, and stool output rhythms when animals are presented with food ad libitum, while restricted feeding reestablishes these rhythms temporarily. In intact mice, chemical sympathectomy with 6-hydroxydopamine has no effect on feeding and locomotor rhythmicity in light-dark cycles or constant darkness but attenuates stool weight and stool number rhythms. Again, however, restricted feeding reestablishes rhythms in locomotor activity, feeding, and stool output rhythms. Ex vivo, intestinal tissue from PER2::LUC transgenic mice expresses circadian rhythms of luciferase bioluminescence. Chemical sympathectomy has little effect on these rhythms, but timed administration of the β-adrenergic agonist isoproterenol causes a phase-dependent shift in PERIOD2 expression rhythms. Collectively, the data suggest that the SCN are required to maintain feeding, locomotor, and stool output rhythms during ad libitum conditions, acting at least in part through daily activation of sympathetic activity. Even so, this input is not necessary for entrainment to timed feeding, which may be the province of oscillators within the intestines themselves or other components of the GI system.

Migratory fat deposition in European quail: a role for prolactin?

1995 ◽  
Vol 146 (1) ◽  
pp. 71-79 ◽  
Author(s):  
T Boswell ◽  
P J Sharp ◽  
M R Hall ◽  
A R Goldsmith
Keyword(s):  
Food Intake ◽  
Body Mass ◽  
Strong Association ◽  
Fat Deposition ◽  
Daily Rhythm ◽  
Plasma Prolactin ◽  
Coturnix Coturnix ◽  
Ad Libitum ◽  
Ovine Prolactin

Abstract The present study addresses the role of prolactin as a regulator of migratory fattening in European quail (Coturnix coturnix). Plasma prolactin levels in captive birds undergoing migratory fattening in an outdoor aviary and in the laboratory were measured by radioimmunoassay with an antibody raised against recombinant-derived chicken prolactin. No strong association between prolactin and migratory fattening was apparent, and prolactin levels were more closely related to daylength, with the highest concentrations being reached on long days. Plasma prolactin profiles were similar in intact and castrated male quail. Prolactin was secreted in a daily rhythm, with the highest concentrations occurring early in the photophase. However, when birds were food-restricted for 50 days during a migratory phase, there was no difference in fat deposition between birds food-deprived for the first half of the daily photophase compared with those deprived for the second half. Fattening was reduced in the food-restricted birds relative to ad libitum-fed controls, but there was no difference in plasma prolactin levels between the groups. Injections of ovine prolactin (4 mg/kg) significantly increased food intake and body mass of birds maintained on long days, but there were no differences in fattening between birds injected in the morning compared with those injected in the afternoon. Collectively, these results do not support a major role for prolactin in the regulation of migratory fat deposition in European quail. Journal of Endocrinology (1995) 146, 71–79

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