Naïve human stem cell medium for maintenance of human induced pluripotent stem (iPS) cells in a naïve state

SummaryWe investigated the effect of human induced pluripotent stem cell (hiPS) medium on porcine somatic cell nuclear transfer and bovine in vitro fertilized early blastocysts, in comparison with North Carolina State University (NCSU)-37 medium and in vitro culture (IVC)-II medium. After 2 days of culture, the diameter of the portion of the blastocyst that was extruded from the zona pellucid dramatically differed between porcine blastocysts cultured in hiPS medium and those cultured in NCSU-37 medium (221.47 ± 38.94 μm versus 481.87 ± 40.61 μm, P < 0.01). Moreover, the diameter of the portion of the blastocyst significantly differed between bovine blastocysts cultured in hiPS medium and those cultured in IVC-II medium (150.30 ± 29.49 μm versus 195.58 ± 41.59 μm, P < 0.01). Furthermore, the total number of cells per porcine and bovine blastocyst was more than two-fold higher in blastocysts cultured in hiPS medium than in those cultured in NCSU-37 medium (44.33 ± 5.28 and 143.33 ± 16.05, P < 0.01) or IVC-II medium (172.12 ± 45.08 and 604.83 ± 242.64, P < 0.01), respectively. These results indicate that hiPS medium markedly improves the quality of porcine and bovine blastocysts.

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A stem cell medium containing neural stimulating factor induces a pancreatic cancer stem-like cell-enriched population

International Journal of Oncology ◽

10.3892/ijo.2014.2603 ◽

2014 ◽

Vol 45 (5) ◽

pp. 1857-1866 ◽

Cited By ~ 12

Author(s):

YUSAKU WATANABE ◽

KIYOSHI YOSHIMURA ◽

KOICHI YOSHIKAWA ◽

RYOICHI TSUNEDOMI ◽

YOSHITARO SHINDO ◽

...

Keyword(s):

Pancreatic Cancer ◽

Stem Cell ◽

Stem Cell Medium ◽

Cell Medium ◽

Stimulating Factor

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Induced Pluripotent Stem Cells: Hope in the Treatment of Diseases, including Muscular Dystrophies

International Journal of Molecular Sciences ◽

10.3390/ijms21155467 ◽

2020 ◽

Vol 21 (15) ◽

pp. 5467

Author(s):

Daniela Gois Beghini ◽

Samuel Iwao Horita ◽

Cynthia Machado Cascabulho ◽

Luiz Anastácio Alves ◽

Andrea Henriques-Pons

Keyword(s):

Stem Cells ◽

Clinical Trials ◽

Stem Cell ◽

Embryonic Stem Cells ◽

Embryonic Stem Cell ◽

Embryonic Stem ◽

Ips Cells ◽

High Plasticity ◽

Cell Based Therapy ◽

Induced Pluripotent

Induced pluripotent stem (iPS) cells are laboratory-produced cells that combine the biological advantages of somatic adult and stem cells for cell-based therapy. The reprogramming of cells, such as fibroblasts, to an embryonic stem cell-like state is done by the ectopic expression of transcription factors responsible for generating embryonic stem cell properties. These primary factors are octamer-binding transcription factor 4 (Oct3/4), sex-determining region Y-box 2 (Sox2), Krüppel-like factor 4 (Klf4), and the proto-oncogene protein homolog of avian myelocytomatosis (c-Myc). The somatic cells can be easily obtained from the patient who will be subjected to cellular therapy and be reprogrammed to acquire the necessary high plasticity of embryonic stem cells. These cells have no ethical limitations involved, as in the case of embryonic stem cells, and display minimal immunological rejection risks after transplant. Currently, several clinical trials are in progress, most of them in phase I or II. Still, some inherent risks, such as chromosomal instability, insertional tumors, and teratoma formation, must be overcome to reach full clinical translation. However, with the clinical trials and extensive basic research studying the biology of these cells, a promising future for human cell-based therapies using iPS cells seems to be increasingly clear and close.

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Selective enhancement of human stem cell proliferation by mussel inspired surface coating

RSC Advances ◽

10.1039/c6ra11173d ◽

2016 ◽

Vol 6 (65) ◽

pp. 60206-60214 ◽

Cited By ~ 6

Author(s):

Xiumei Jiang ◽

Yanfang Li ◽

Ying Liu ◽

Chunying Chen ◽

Menglin Chen

Keyword(s):

Stem Cell ◽

Surface Coating ◽

Pluripotent Stem Cell ◽

Induced Pluripotent Stem Cell ◽

Surface Coatings ◽

Human Stem Cell ◽

Adhesion Properties ◽

Stem Cell Proliferation ◽

Induced Pluripotent ◽

Selective Enhancement

The biocompatibility and cell adhesion properties of mussel inspired polydopamine and polynorepinephrine surface coatings on PCL fibers for human mesenchymal and human induced pluripotent stem cell derived mesenchymal stem cells were investigated.

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Identification of a native novel oncolytic immunoglobulin on exfoliated colon epithelial cells: A bispecific heterodimeric chimera of IgA/IgG

10.1101/140673 ◽

2017 ◽

Author(s):

George P. Albaugh ◽

Sudhir K. Dutta ◽

Vasantha Iyengar ◽

Samina Shami ◽

Althaf Lohani ◽

...

Keyword(s):

Stem Cell ◽

Direct Method ◽

Stem Cell Markers ◽

Distinct Cell ◽

Stem Cell Medium ◽

Stool Samples ◽

Low Levels ◽

Colonic Cells ◽

Cell Medium ◽

A Direct Method

ABSTRACTUnderstanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5μM– 5.0 μM and 5.0μM-8.0μM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathophysiology and a direct method to create a database for applications in regenerative medicine.

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Induced pluripotent stem cells: the long-expected revolution in medical science and practice?

Journal of Nucleic Acids Investigation ◽

10.4081/jnai.2010.1625 ◽

2010 ◽

Vol 1 (1) ◽

pp. 1

Author(s):

Antonio Sorrentino

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Regenerative Medicine ◽

Stem Cell Research ◽

Pluripotent Stem Cells ◽

Medical Science ◽

Ips Cells ◽

Research Field ◽

Toxicology Screening ◽

Induced Pluripotent

Within the matter of a few years, development of the somatic reprogramming technology to generate induced pluripotent stem (iPS) cells has contributed enormously to the stem cell research field. We learned that differentiated adult cells possess an unrestricted plasticity that allows them to be driven back to their embryonic or pluripotent state, but owing to the juvenile nature of this novel science chapter, there are many unanswered questions and dilemmas. It is indisputable, however, that iPS cells potentially could represent the jack-of-all-trades remedy in areas of medicine ranging from toxicology screening to regenerative medicine. In this review I will summarize the current strategies employed to reprogram somatic cells and the major promises and hurdles for the future of iPS cells.

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EVI1 Is Activated During the Generation of Induced Pluripotent Stem (iPS) Cells.

Blood ◽

10.1182/blood.v114.22.5048.5048 ◽

2009 ◽

Vol 114 (22) ◽

pp. 5048-5048

Author(s):

Leopoldo Laricchia-Robbio ◽

Nuria Montserrat ◽

Alessandra Giorgetti ◽

Juan Carlos Izpisúa Belmonte

Keyword(s):

Stem Cell ◽

Conflicts Of Interest ◽

Integration Site ◽

Ips Cells ◽

The Self ◽

Genomic Locus ◽

Self Renewal ◽

Hematopoietic Stem ◽

Retroviral Integration ◽

Induced Pluripotent

Abstract Abstract 5048 EVI1 gene was first identified as a common site of retroviral integration in murine leukemia models. This gene is part of a complex genomic locus, MDS1-EVI1, that has been described as a target for retroviral integration that may lead to the emergence of a non-malignant dominant hematopoietic stem cell (HSC) clone in mice, in primates, and in humans. These studies suggested that one of the genes encoded by this locus could affect the self-renewal potential of HSC. Recent studies in mice revealed that indeed EVI1 plays an essential role in cell proliferation and it also enhances the self-renewal ability of HSC. The intense attention focused on the MDS1-EVI1 locus as retrovirus integration site prompted us to investigate whether EVI1 might have a role in somatic cell re-programming generated with retroviruses. Recent developments in stem cell research have enabled the re-programming of somatic cells to a pluripotent state using exogenous factors. Induced pluripotent stem (iPS) cells have the potential to differentiate into any cells types and that might be used in the future for clinical therapy. In order to elucidate the molecular events allowing the conversion of adult somatic into pluripotent stem cell, we evaluated EVI1 expression during this process. We found that EVI1 is activated in the early stages of re-programming and then it is silenced once the cells has been fully re-programmed. EVI1 seems to facilitate the initiation of cell re-programming by up-regulating a subset of genes previously described as potent stimulators of stem cells expansion. Disclosures No relevant conflicts of interest to declare.

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