Abstract
Osteoarthritis (OA) is the most common form of degenerative joint disease, affecting more than 25% of the adult though prevalent in the elderly population. Most of the current therapeutic modalities aim at symptomatic treatment and lingering the disease progression. In recent years, regenerative medicine such as stem cell transplantation and tissue engineering has been suggested as a potential curative intervention for OA. The objective of current study was to assess the safety and efficacy of an injectable tissue-engineered construct composed of BMMSCs, PRP, and Collagen type I in rat model of OA.To produce collagen type I, PRP and BMMSCs, male Wistar rats were ethically euthanized. After expansion and characterization of rat BMMSCs (rBMMSCs), tissue-engineered construct was formed by combination of appropriate amount of collagen type I, PRP and rBMMSCs. In vitro studies were conducted to evaluate the effect of PRP on chondrogenic differentiation capacity of encapsulated cells. Then tissue-engineered construct was injected in knee joint of rat model of OA (24 rat in 4 groups:OA, OA+MSC, OA+Collagen+MSC+PRP, OA+MSC+Collagen).After 6 weeks, the animals were euthanized and knee joint histopathology examinations were performed to evaluate the effect of each treatment on OA.Tissue-engineered construct was successfully manufactured and in vitro assays demonstrated that relevant chondrogenic genes and proteins expression were higher in PRP group than the others. Histopathological findings of the knee joint samples showed favorable regenerative effect of rBMMSCs+PRP+Collagengroup comparing to others.In this study, we introduced an injectable tissue-engineered product composed of rBMMSCs+PRP+Collagenwith potential regenerative effect on cartilage damage caused by OA.