scholarly journals A stem cell medium containing neural stimulating factor induces a pancreatic cancer stem-like cell-enriched population

2014 ◽  
Vol 45 (5) ◽  
pp. 1857-1866 ◽  
Author(s):  
YUSAKU WATANABE ◽  
KIYOSHI YOSHIMURA ◽  
KOICHI YOSHIKAWA ◽  
RYOICHI TSUNEDOMI ◽  
YOSHITARO SHINDO ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cell ◽  
Stem Cell Medium ◽  
Cell Medium ◽  
Stimulating Factor

Generation of large pig and bovine blastocysts by culturing in human induced pluripotent stem cell medium

Zygote ◽  
2015 ◽  
Vol 24 (2) ◽  
pp. 236-244 ◽  
Author(s):  
Qing-Shan Gao ◽  
Long Jin ◽  
Suo Li ◽  
Hai-Ying Zhu ◽  
Qing Guo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
State University ◽  
North Carolina State University ◽  
Stem Cell Medium ◽  
Induced Pluripotent ◽  
Cell Medium

SummaryWe investigated the effect of human induced pluripotent stem cell (hiPS) medium on porcine somatic cell nuclear transfer and bovine in vitro fertilized early blastocysts, in comparison with North Carolina State University (NCSU)-37 medium and in vitro culture (IVC)-II medium. After 2 days of culture, the diameter of the portion of the blastocyst that was extruded from the zona pellucid dramatically differed between porcine blastocysts cultured in hiPS medium and those cultured in NCSU-37 medium (221.47 ± 38.94 μm versus 481.87 ± 40.61 μm, P < 0.01). Moreover, the diameter of the portion of the blastocyst significantly differed between bovine blastocysts cultured in hiPS medium and those cultured in IVC-II medium (150.30 ± 29.49 μm versus 195.58 ± 41.59 μm, P < 0.01). Furthermore, the total number of cells per porcine and bovine blastocyst was more than two-fold higher in blastocysts cultured in hiPS medium than in those cultured in NCSU-37 medium (44.33 ± 5.28 and 143.33 ± 16.05, P < 0.01) or IVC-II medium (172.12 ± 45.08 and 604.83 ± 242.64, P < 0.01), respectively. These results indicate that hiPS medium markedly improves the quality of porcine and bovine blastocysts.

scholarly journals Identification of a native novel oncolytic immunoglobulin on exfoliated colon epithelial cells: A bispecific heterodimeric chimera of IgA/IgG

2017 ◽  
Author(s):  
George P. Albaugh ◽  
Sudhir K. Dutta ◽  
Vasantha Iyengar ◽  
Samina Shami ◽  
Althaf Lohani ◽  
...  
Keyword(s):  
Stem Cell ◽  
Direct Method ◽  
Stem Cell Markers ◽  
Distinct Cell ◽  
Stem Cell Medium ◽  
Stool Samples ◽  
Low Levels ◽  
Colonic Cells ◽  
Cell Medium ◽  
A Direct Method

ABSTRACTUnderstanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5μM– 5.0 μM and 5.0μM-8.0μM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathophysiology and a direct method to create a database for applications in regenerative medicine.

scholarly journals Hypoxic-Preconditioned Bone Marrow Stem Cell Medium Significantly Improves Outcome After Retinal Ischemia in Rats

2016 ◽  
Vol 57 (7) ◽  
pp. 3522 ◽  
Author(s):  
Steven Roth ◽  
John C. Dreixler ◽  
Biji Mathew ◽  
Irina Balyasnikova ◽  
Jacob R. Mann ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Retinal Ischemia ◽  
Bone Marrow Stem Cell ◽  
Stem Cell Medium ◽  
Cell Medium ◽  
Marrow Stem Cell

scholarly journals Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF

2013 ◽  
Vol 47 (4) ◽  
pp. 330-337 ◽  
Author(s):  
Neza Podergajs ◽  
Narve Brekka ◽  
Bernhard Radlwimmer ◽  
Christel Herold-Mende ◽  
Krishna M. Talasila ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Basal Medium ◽  
Growth Stimulation ◽  
Growth Conditions ◽  
Stem Cell Markers ◽  
Egfr Amplification ◽  
Egfr Expression ◽  
Stem Cell Medium ◽  
Cell Medium

Abstract Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without EGF, as the presence of EGF induced rapid loss of EGFR amplification. However, patient biopsies are usually taken into culture before their genomic profiles are defined. Thus, an important question remains whether GBM cells without EGFR amplification also can be cultured in stem cell medium without EGF. Meterials and methods. To address this question, we used two heterogeneous glioblastoma GSC lines (NCH421k and NCH644) that lack EGFR amplification. Results. Although both cell lines showed very low EGFR expression under standard growth conditions, bFGF stimulation induced higher expression of EGFR in NCH644. In both cell lines, expression of the stem cell markers nestin and CD133 was higher upon stimulation with bFGF compared to EGF. Importantly, bFGF stimulated the growth of both cell lines, whereas EGF had no effect. We verified that the growth stimulation by bFGF was either mediated by proliferation (NCH421k) or resistance to apoptosis (NCH644). Conclusions. We demonstrate that GSC cultures without EGFR amplification can be maintained and expanded with bFGF, while the addition of EGF has no significant effect and therefore can be omitted.

scholarly journals Osteoclastogenic Potential of Tissue-Engineered Periosteal Sheet: Effects of Culture Media on the Ability to Recruit Osteoclast Precursors

2021 ◽  
Vol 22 (4) ◽  
pp. 2169
Author(s):  
Kohya Uematsu ◽  
Takashi Ushiki ◽  
Hajime Ishiguro ◽  
Riuko Ohashi ◽  
Suguru Tamura ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fluorescent Protein ◽  
Bone Marrow Cells ◽  
Culture Media ◽  
Γ Irradiation ◽  
Stem Cell Medium ◽  
Cell Medium ◽  
Conventional Medium

Cell culture media influence the characteristics of human osteogenic periosteal sheets. We have previously found that a stem cell medium facilitates growth and collagen matrix formation in vitro and osteogenesis in vivo. However, it has not yet been demonstrated which culture medium is superior for osteoclastogenesis, a prerequisite for reconstruction of normal bone metabolic basis. To address this question, we compared chemotaxis and osteoclastogenesis in tissue-engineered periosteal sheets (TPSs) prepared with two types of culture media. Periosteal tissues obtained from adult volunteers were expanded with the conventional Medium 199 or with the stem cell medium, MesenPRO. Hematopoietic enhanced-green-fluorescent-protein (EGFP)-nude mice were prepared by γ-irradiation of Balb/c nu/nu mice and subsequent transplantation of bone marrow cells from CAG-EGFP C57BL/6 mice. TPSs were implanted subcutaneously into the chimeric mice and retrieved after intervals for immunohistopathological examination. EGFP+ cells were similarly recruited to the implantation site in both the TPSs prepared, whereas the distribution of CD11b+ cells was significantly lower in the TPS prepared with the stem cell medium. Instead, osteoclastogenesis was higher in the TPS prepared with the stem cell medium than in the one prepared with the conventional medium. These findings suggest that the stem cell medium is preferable for the preparation of more functional TPSs.

scholarly journals Overexpression of Endogenous Retroviruses and Malignancy Markers in Neuroblastoma Cell Lines by Medium-Induced Microenvironmental Changes

2021 ◽  
Vol 11 ◽  
Author(s):  
Lisa Wieland ◽  
Kristina Engel ◽  
Ines Volkmer ◽  
Anna Krüger ◽  
Guido Posern ◽  
...  
Keyword(s):  
Stem Cell ◽  
Quantitative Pcr ◽  
Cell Lines ◽  
Transcriptional Activation ◽  
Neuroblastoma Cell ◽  
Endogenous Retrovirus ◽  
Endogenous Retroviruses ◽  
Tumor Escape ◽  
Stem Cell Medium ◽  
Cell Medium

Neuroblastoma (NB) is the commonest solid tumor outside the central nervous system in infancy and childhood with a unique biological heterogeneity. In patients with advanced, metastasizing neuroblastoma, treatment failure and poor prognosis is often marked by resistance to chemo- or immunotherapy. Thus, identification of robust biomarkers seems essential for understanding tumor progression and developing effective therapy. Here, we have studied the expression of human endogenous retroviruses (HERV) as potential targets in NB cell lines during stem-cell medium-induced microenvironmental change. Quantitative PCR revealed that relative expression of the HERV-K family and HERV-W1 ENV were increased in all three NB cell lines after incubation in stem-cell medium. Virus transcriptome analyses revealed the transcriptional activation of three endogenous retrovirus elements: HERV-R ENV (ERV3-1), HERV-E1 and HERV-Fc2 ENV (ERVFC1-1). Known malignancy markers in NB, e.g. proto-oncogenic MYC or MYCN were expressed highly heterogeneously in the three investigated NB cell lines with up-regulation of MYC and MYCN upon medium-induced microenvironmental change. In addition, SiMa cells exclusively showed a phenotype switching from loosely-adherent monolayers to low proliferating grape-like cellular aggregates, which was accompanied by an enhanced CD133 expression. Interestingly, the overexpression of HERV was associated with a significant elevation of immune checkpoint molecule CD200 in both quantitative PCR and RNA-seq analysis suggesting tumor escape mechanism in NB cell lines after incubation in serum-free stem cell medium.

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