Interleukin-10 gene promoter polymorphism in English and Polish healthy controls. Polymerase chain reaction haplotyping using 3′ mismatches in forward and reverse primers

The promoter region of human Interleukin −10 gene is highly polymorphic and has been associated with numerous autoimmune diseases. Recent studies have linked vitiligo with defective autoimmune system. This study is aimed to explore a possible association between IL-10 gene polymorphism and vitiligo in Saudi population. This case control study consisted of 184 Saudi subjects including 83 vitiligo patients (40 males, 43 females mean age 27.85 ± 12.43 years) and 101 matched controls. Genomic DNA was extracted from the blood samples of healthy controls and Vitiligo patients visiting out patient clinic of Department of Dermatology, Riyadh Armed Forces Hospital, using QIA ampRDNA mini kit (Qiagen CA, USA). Interleukin-10 gene was amplified by polymerase chain reaction (PCR) using Arms primers to detect any polymorphism involved at positions −592, −819 and −1082.The frequencies of GG genotype at −1082, and CC genotype at positions −592 and 819 were significantly higher in vitiligo patients compared to healthy subjects suggesting that GG and CC genotypes might be susceptible to vitiligo in Saudis. On the other hand genotypes −1082 GA, −819 CT, and −592 CA of IL-10 were more prevalent in healthy controls suggesting protective effects of GA, CT and CA genotypes against vitiligo. This study indicates that the IL-10 gene may play a significant role in the etiology of vitiligo among Saudis.

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AB0691 ANALYSIS OF SARS-COV-2 ANTIBODIES IN NON-COVID-19 PATIENTS: COMPARISON BETWEEN SYSTEMIC SCLEROSIS PATIENTS AND HEALTHY CONTROLS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.3143 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 1379.1-1379

Author(s):

L. Giardullo ◽

C. Rotondo ◽

A. Corrado ◽

N. Maruotti ◽

R. Colia ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Systemic Sclerosis ◽

Autoimmune Disease ◽

Reverse Transcriptase ◽

Real Time ◽

Polymerase Chain Reaction Assay ◽

Nuclear Antigen ◽

Healthy Controls ◽

Chain Reaction ◽

Polymerase Chain

Background:Previous study evidenced a cross-reactivity between Sars-Cov-2 antibodies and autoimmune tissue antigen involved in connective tissue diseases, as nuclear antigen (NA), extractable nuclear antigen (ENA), histone and collagen (1). No study has been published about the titer of Sars-Cov-2 antibodies in non-infected patients with autoimmune disease.Objectives:To evaluate the titer of SARS-CoV-2 antibodies in non-COVID-19 patients and compare it between systemic sclerosis (SSc) patients and healthy controls (HC).Methods:A total of 58 patients with SSc (who fulfilled ACR/EULAR 2013 SSc classification criteria) and 18 HC were enrolled. Sera of all participants were collected, and SARS-CoV-2 antibodies (IgG and IgM) were evaluated by means ELISA. In all participants swabs for SARS-CoV-2 by real-time reverse-transcriptase-polymerase-chain-reaction assay were reported negative. Demographic, clinical, and autoimmune serological characteristics of SSc patients were recorded. The normal distribution was assessed using the Shapiro–Wilk’s test. Exclusion criteria was previous or actual Sars-Cov-2 infection. Comparisons between study groups of patients were evaluated by the Student’s t-test or Mann – Whitney U-test as appropriate. The differences between categorial variables were assessed by Pearson chi-square or Fisher’s exact test, as opportune. Statistical significance was set at p ≤ 0.05.Results:We observed significant differences between SSc patients and HC in serum levels of Sars-Cov-2 antibodies (IgG: 1,4±2,1 AU/ml vs 0,36±0,19 AU/ml respectively (p=0,001); and IgM: 2,5±3,1 AU/ml vs 0,8±0,7 AU/ml (p=0,022)). In 5 SSc patients was found titer of Sars-Cov-2 antibodies (IgG) exceeding the cut-off, but the control of swabs for SARS-CoV-2 by real-time reverse-transcriptase-polymerase-chain-reaction assay were negative. No significative differences in Sars-Cov-2 autoantibodies titer were found in subgroup of SSc patients with or without ILD or PAH, limited or diffuse skin subset, and different autoantibodies profile. Furthermore, antibodies titer was not associated with different drugs (steroid, methotrexate, mofetil-mycophenolate and bosentan) in use.Conclusion:A cross mimicking between Sars-Cov-2 antibodies and antinuclear antibodies or anti ENA could be hypothesized. Further studies are necessary to unravel the reliability of Sars-Cov-2 antibodies detection in autoimmune disease.References:[1]Vojdani, A., Vojdani, E., & Kharrazian, D. (2021). Reaction of human monoclonal antibodies to SARS-CoV-2 proteins with tissue antigens: Implications for autoimmune diseases. Frontiers in Immunology, 11, 3679Disclosure of Interests:None declared

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MEASUREMENT OF IL-12 (P40, P35), IL-23P19 AND IFN-MRNA IN DUODENAL BIOPSIES OF CATS WITH IBD AND HEALTHY CONTROLS USING QUANTITATIVE REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (QRT-PCR)

Mansoura Veterinary Medical Journal ◽

10.21608/mvmj.2016.129719 ◽

2016 ◽

Vol 17 (2) ◽

pp. 25-33

Author(s):

WALY E ◽

BIOURGE V ◽

Iain Peters ◽

DAY J ◽

STOKES R ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Reverse Transcriptase ◽

Healthy Controls ◽

Chain Reaction ◽

Qrt Pcr ◽

Polymerase Chain ◽

Duodenal Biopsies

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Study of the HLA-DPβ1 Locus by the Polymerase Chain Reaction Technique in Patients with Hodgkin's Disease

Tumori Journal ◽

10.1177/030089169307900211 ◽

1993 ◽

Vol 79 (2) ◽

pp. 133-136 ◽

Cited By ~ 2

Author(s):

Guseppe Pellegris ◽

Claudia Lombardo ◽

Annelisa Cantoni ◽

Liliana Devizzi ◽

Monica Balzarotti

Keyword(s):

Polymerase Chain Reaction ◽

Hodgkin's Disease ◽

Hodgkin’S Disease ◽

Polymerase Chain Reaction Method ◽

Healthy Controls ◽

Chain Reaction ◽

Reaction Method ◽

Significant Difference ◽

Polymerase Chain

Background A number of reports have studied associations between Hodgkin's disease and HLA. Some of them established correlation between several antigens and Hodgkin's disease, and others found no correlations. Methods The HLA DP locus was determined by the polymerase chain reaction method in 31 Hodgkin's disease patients and 58 healthy controls. Results No significant difference between patients and controls was noted. Conclusions Further investigations are needed to confirm the hypothesis of a possible role of the HLA complex as one of the factors involved in Hodgkin's disease.

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Heparanase Overexpresses in Keratoconic Cornea and Tears Depending on the Pathologic Grade

Disease Markers ◽

10.1155/2017/3502386 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Beatriz García ◽

Olivia García-Suárez ◽

Jesús Merayo-Lloves ◽

Guilherme Ferrara ◽

Ignacio Alcalde ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Catalytic Activity ◽

Enzymatic Activity ◽

Inflammatory Markers ◽

Quantitative Polymerase Chain Reaction ◽

Healthy Controls ◽

Inflammatory Condition ◽

Chain Reaction ◽

Polymerase Chain ◽

Corneal Cells

Background. Keratoconus has classically been defined as a noninflammatory disorder, although recent studies show elevated levels of inflammatory markers suggesting that keratoconus could be, at least in part, an inflammatory condition. Heparanase upregulation has been described in multiple inflammatory disorders. In this article, we study the differential expression of heparanase in cornea and tears from keratoconus patients and healthy controls. Methods. A transcriptomic approach was used employing quantitative polymerase chain reaction to analyze the expression of heparanase and heparanase 2 in stromal and epithelial corneal cells. The protein expression was analyzed by immunohistochemistry in corneal sections. Enzymatic activity in tears was measured using [3H]-labeled heparan sulfate as substrate. Results. Heparanase transcription was detected in stromal and epithelial cells and appeared upregulated in keratoconus. Overexpression of the enzyme was also detected by immunohistochemistry. Corneal expression of heparanase 2 was detected in some cases. Heparanase catalytic activity was found in tears and displayed a positive correlation with the degree of keratoconus. Conclusions. Heparanase overexpresses in keratoconic corneas, possibly reinforcing the inflammatory condition of the pathology. The presence of heparanase activity in tears allows us to propose its use as a biomarker for the diagnosis of the disorder.

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