scholarly journals The role of p38 MAPK in neutrophil functions: single cell chemotaxis and surface marker expression

The Analyst ◽  
2013 ◽  
Vol 138 (22) ◽  
pp. 6826 ◽  
Author(s):  
Donghyuk Kim ◽  
Christy L. Haynes
Keyword(s):  
Single Cell ◽  
P38 Mapk ◽  
Surface Marker ◽  
Surface Marker Expression ◽  
Marker Expression ◽  
Cell Chemotaxis

Engraftability of Murine Bone Marrow-Derived Multipotent Mesenchymal Stem Cell Subpopulations in the Tissues of Developing Mice following Systemic Transplantation

2015 ◽  
Vol 201 (1) ◽  
pp. 14-25 ◽  
Author(s):  
Feng Li ◽  
Christopher Niyibizi
Keyword(s):  
Bone Marrow ◽  
Single Cell ◽  
Circulatory System ◽  
Surface Marker ◽  
Murine Bone Marrow ◽  
Surface Marker Expression ◽  
Donor Cells ◽  
Marker Expression ◽  
Cell Subpopulations ◽  
Different Tissues

Introduction: Cell therapies for generalized musculoskeletal diseases would require distribution of cells to all the skeletal tissues; however, there are controversies regarding the transplantability of multipotent mesenchymal stems cells (MSCs). We generated single-cell subpopulations of MSCs from murine bone marrow and assessed them for differences in trafficking through the circulatory system and engraftment in bone and other tissues. Materials and Methods: Seven single-cell clonal subpopulations were generated by serial dilution of GFP-marked MSCs isolated from bone marrow. The subpopulations were examined for putative MSC surface marker expression, in vitro differentiation toward osteogenic and adipogenic lineages, migration and engraftment in different tissues following intravenous delivery in normal, sublethally irradiated neonatal mice. Results: The surface marker expression profile revealed notable differences among clonal cells, specifically CD44 and CD105. All the cell subpopulations differentiated toward osteogenic and adipogenic lineages, with some committed to only one or the other. Two clones enriched in CXCR4 expression were highly efficient in migrating and engrafting in skeletal tissue including bone; this confirmed the role of this chemokine in cell migration. Donor cells retrieved from various tissues displayed different morphologies and potential differentiation into tissue cell type of engraftment, suggesting modification by the tissues in which the donor cells engrafted. Conclusion: We have reported that, within bone marrow, there are heterogeneous subpopulations of MSCs that may differ in their ability to migrate in the circulatory system and engraft in different tissues.

Interleukin-1β and surface marker expression changes induced by tetrachloroplatinate in human monocyte-derived dendritic cells

2009 ◽  
Vol 00 (00) ◽  
pp. 090824065221002-10
Author(s):  
Joanna Arkusz ◽  
Dobrosława Gradecka-Meesters ◽  
Maciej Stępnik
Keyword(s):  
Dendritic Cells ◽  
Human Monocyte ◽  
Surface Marker ◽  
Interleukin 1Β ◽  
Surface Marker Expression ◽  
Marker Expression

scholarly journals POS0333 ALTERED MACROPHAGE POLARIZATION PHENOTYPES IN SYSTEMIC SCLEROSIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 394.1-394
Author(s):  
A. Hukara ◽  
M. Rudnik ◽  
C. B. Rufer ◽  
O. Distler ◽  
P. Blyszczuk ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Macrophage Polarization ◽  
Peritoneal Macrophages ◽  
Surface Marker ◽  
Surface Marker Expression ◽  
Tnf Α ◽  
Ifn Γ ◽  
Alternatively Activated ◽  
Classically Activated

Background:Fos-like 2 (Fosl-2) is a transcription factor of the AP-1 family and has a broad range in inducing cellular changes affecting fibrosis and inflammatory responses. Pathological effects of Fosl-2 have been associated with systemic sclerosis (SSc). In addition, increased expression of Fosl-2 has been detected in human SSc monocyte-derived macrophages [1]. Monocytes and macrophages play a central role in activating and propagating acute inflammation followed by pathological fibrosis and organ dysfunction. The classification of the macrophage polarization phenotype can be assigned based on the stimulus, for example into classically-activated M(LPS), and alternatively-activated M(IL-4) macrophages [2]. However, the role of the Fosl-2 transcription factor in macrophage polarization remains elusive.Objectives:To investigate the role of Fosl-2 in macrophage polarization in SSc using Fosl-2 overexpressing transgenic (Fosl-2 tg) mice and human blood-derived macrophages from SSc patients.Methods:Thiogylcolate-elicited peritoneal macrophages were isolated from wild-type (wt) and Fosl-2 tg mice. Human peripheral CD14+ blood-derived monocytes were isolated and differentiated to macrophages (hMDM) from healthy controls and SSc patients. Murine and human macrophages were polarized with LPS (10 ng/ml), LPS + recombinant mouse IFN-γ (10 ng/ml), recombinant mouse, resp. human IL-4 (10 ng/ml) or remained untreated. Macrophage surface marker expression was assessed by flow cytometry using a mouse (F4/80, CD11b, CD86, CD80, CD38, MHCII, CD206, PD-L1, PD-L2, CD36) or human (CD38, CD40, CD86, PD-L2, PD-L1, CD163, CD206) designed polarization panel. Phagocytic activity was detected with pHrodo Red E.coli particles by flow cytometry. Gene expression and secretion of pro- and anti-inflammatory markers were measured by RT-qPCR, standard ELISAs and Griess Assay for nitric oxide production.Results:After LPS stimulation, mRNA levels of IL-1β (p<0.01, n=11-12), TNF-α (p=0.05, n=11-12) and IFN-γ (p<0.05, n=7) were reduced, whereas expression of IL-10 (p<0.05, n=11-12) was enhanced in Fosl-2 tg peritoneal macrophages in comparison to wt cells. Secretion of TNF-α (p<0.01, n=9-11) and nitric oxide (p<0.01, n=9) was impaired in Fosl-2 tg peritoneal macrophages compared to wt cells after LPS stimulation. Peritoneal macrophages were analyzed directly after isolation for macrophage polarization cell surface marker expression. Fosl-2 tg peritoneal macrophages showed an increase in the F4/80+CD11b+PD-L2+CD36+ cell population (p<0.01, n=3-6) compared to peritoneal macrophages from wt mice.The expression of cell surface markers of non-polarized and IL-4 stimulated SSc hMDM (n=17) showed an increased percentage of CD40+CD86+CD206+PD-L2+CD163+ cells (p<0.05) compared to healthy control hMDM (n=7). Phagocytic activity was enhanced in SSc hMDM (n=7) compared to healthy untreated (p<0.05), LPS (p=0.05) and IL-4 (p<0.05) hMDM (n=5).Conclusion:Our animal data indicates a role of Fosl-2 in regulating macrophage polarization with a shift from a classically-activated to an alternatively-activated phenotype. Similarly, SSc hMDM resemble a functional M(IL-4) alternative macrophage phenotype.Thus, maintaining a balanced proportion of classically- and alternatively-activated macrophage phenotypes may be an effective tool to control macrophage function in SSc.References:[1]Moreno-Moral, A., et al., Changes in macrophage transcriptome associate with systemic sclerosis and mediate GSDMA contribution to disease risk. Ann Rheum Dis, 2018. 77(4): p. 596-601.[2]Kania, G., M. Rudnik, and O. Distler, Involvement of the myeloid cell compartment in fibrogenesis and systemic sclerosis. Nat Rev Rheumatol, 2019. 15(5): p. 288-302.Disclosure of Interests:Amela Hukara: None declared, Michal Rudnik: None declared, Chantal Brigitta Rufer: None declared, Oliver Distler Speakers bureau: Actelion, Bayer, Boehringer Ingelheim, Medscape, Novartis, Roche, Menarini, Mepha, MSD, iQone, Pfizer, Consultant of: Abbvie, Actelion, Acceleron Pharma, Amgen, AnaMar, Arxx Therapeutics, Bayer, Baecon Discovery, Blade Therapeutics, Boehringer, CSL Behring, ChemomAb, Corpuspharma, Curzion Pharmaceuticals, Ergonex, Galapagos NV, GSK, Glenmark Pharmaceuticals, Inventiva, Italfarmaco, iQvia, Kymera, Medac, Medscape, Mitsubishi Tanabe Pharma, MSD, Roche, Sanofi, UCB, Lilly, Target BioScience, Pfizer, Grant/research support from: Actelion, Bayer, Boehringer Ingelheim, Kymera Therapeutics, Mitsubishi Tanabe, Przemyslaw Blyszczuk: None declared, Gabriela Kania: None declared

scholarly journals FACS-Based Isolation, Propagation and Characterization of Mouse Embryonic Cardiomyocytes Based on VCAM-1 Surface Marker Expression

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e82403 ◽  
Author(s):  
Annica Pontén ◽  
Stuart Walsh ◽  
Daniela Malan ◽  
Xiaojie Xian ◽  
Susanne Schéele ◽  
...  
Keyword(s):  
Surface Marker ◽  
Surface Marker Expression ◽  
Embryonic Cardiomyocytes ◽  
Marker Expression

scholarly journals Effect of pregnancy hormone mixtures on cytokine production and surface marker expression in naïve and LPS-activated THP-1 differentiated monocytes/macrophages

2019 ◽  
Vol 26 (2) ◽  
pp. 84-96
Author(s):  
María Isabel Mendoza-Cabrera ◽  
Rosa-Elena Navarro-Hernández ◽  
Anne Santerre ◽  
Pablo Cesar Ortiz-Lazareno ◽  
Ana Laura Pereira-Suárez ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Cytokine Production ◽  
Immune Cell ◽  
Surface Marker ◽  
Independent Manner ◽  
Surface Marker Expression ◽  
Monocytes And Macrophages ◽  
Marker Expression ◽  
Activated Monocytes ◽  
In Pregnancy

In pregnancy, maternal monocytes and macrophages acquire a specific phenotype that enables them to maintain immune tolerance and facilitate hormone–immune cell interactions, which are necessary for gestational progression. The aim of this study was to determine the effect of pregnancy hormone mixtures of the first and third trimesters on both resting and activated monocytes and macrophages. Pregnancy hormone levels (cortisol, estradiol, progesterone, and prolactin) were quantified at the first and third trimesters. The average of the levels obtained was used to prepare two mixtures of synthetic hormones: low and high. These mixtures were then used to stimulate THP-1 monocytes and macrophages, resting or activated with LPS. Cytokine production in the culture supernatants and surface marker expression (CD14, CD86, and CD163) were evaluated by ELISA and flow cytometry, respectively. We found that the hormones modulated the pro-inflammatory response of THP-1 cells, LPS-activated monocytes, and macrophages, inducing high levels of IL-10 and low levels of IL-8, IL-1-β, and IL-6. All hormone stimulation increased the CD163 receptor in both resting and LPS-activated monocytes and macrophages in a dose-independent manner, unlike CD14 and CD86. Pregnancy hormones promote the expression of the markers associated with the M2-like phenotype, modulating their pro-inflammatory response. This phenotype regulation by hormones could be a determinant in pregnancy.

Tu1234 Granulomas of Intestinal Tuberculosis and Crohns Disease Can Be Differentiated by CD 73 Mesenchymal Cell Surface Marker Expression

2012 ◽  
Vol 142 (5) ◽  
pp. S-780-S-781
Author(s):  
Rupa Banerjee ◽  
M. Balaji ◽  
Sasikala Mitnala ◽  
Sekaran Anuradha ◽  
D. Nageshwar Reddy
Keyword(s):  
Cell Surface ◽  
Intestinal Tuberculosis ◽  
Mesenchymal Cell ◽  
Surface Marker ◽  
Cell Surface Marker ◽  
Crohns Disease ◽  
Surface Marker Expression ◽  
Marker Expression ◽  
Cell Surface Marker Expression

scholarly journals Quantifying Cell-Surface Marker Expression Through Imaging of Transient Interactions

2014 ◽  
Vol 106 (2) ◽  
pp. 245a
Author(s):  
Mustafa A.H. Mir ◽  
Olivia Scheideler ◽  
Jeremy Whang ◽  
Lydia L. Sohn
Keyword(s):  
Cell Surface ◽  
Surface Marker ◽  
Cell Surface Marker ◽  
Surface Marker Expression ◽  
Transient Interactions ◽  
Marker Expression ◽  
Cell Surface Marker Expression

Differences in Surface Marker Expression and Chondrogenic Potential among Various Tissue-Derived Mesenchymal Cells from Elderly Patients with Osteoarthritis

2012 ◽  
Author(s):  
Elena Alegre-Aguarón ◽  
Paula Desportes ◽  
Felícito García-Álvarez ◽  
Tomás Castiella ◽  
Luis Larrad ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Mesenchymal Cells ◽  
Surface Marker ◽  
Surface Marker Expression ◽  
Chondrogenic Potential ◽  
Marker Expression
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