Silk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineering

2015 ◽  
Vol 7 (1) ◽  
pp. 53-63 ◽  
Author(s):  
Nandana Bhardwaj ◽  
Wan Ting Sow ◽  
Dipali Devi ◽  
Kee Woei Ng ◽  
Biman B. Mandal ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Silk Fibroin ◽  
Skin Tissue ◽  
Dermal Substitute ◽  
Skin Substitutes ◽  
3D Scaffolds ◽  
Skin Tissue Engineering ◽  
Dermal Tissue ◽  
Engineered Skin Substitutes

Development of highly vascular dermal tissue-engineered skin substitutes with appropriate mechanical properties and cellular cues is in need for significant advancement in the field of dermal reconstruction.

scholarly journals Correction: Silk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineering

2015 ◽  
Vol 7 (1) ◽  
pp. 142-142 ◽  
Author(s):  
Nandana Bhardwaj ◽  
Wan Ting Sow ◽  
Dipali Devi ◽  
Kee Woei Ng ◽  
Biman B. Mandal ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Skin Tissue ◽  
Dermal Substitute ◽  
3D Scaffolds ◽  
Skin Tissue Engineering

Correction for ‘Silk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineering’ by Nandana Bhardwaj et al., Integr. Biol., 2015, DOI: 10.1039/c4ib00208c.

Preparation and characterization of polyhydroxybutyrate-co -hydroxyvalerate/silk fibroin nanofibrous scaffolds for skin tissue engineering

2014 ◽  
Vol 55 (4) ◽  
pp. 907-916 ◽  
Author(s):  
Caihong Lei ◽  
Hailin Zhu ◽  
Jingjing Li ◽  
Jiuming Li ◽  
Xinxing Feng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds

scholarly journals Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 118
Author(s):  
Shima Tavakoli ◽  
Marta A. Kisiel ◽  
Thomas Biedermann ◽  
Agnes S. Klar
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Skin Wound ◽  
Skin Tissue ◽  
Diabetic Patients ◽  
Specific Cell ◽  
Skin Substitutes ◽  
Skin Wound Healing ◽  
Skin Tissue Engineering

The immune system has a crucial role in skin wound healing and the application of specific cell-laden immunomodulating biomaterials emerged as a possible treatment option to drive skin tissue regeneration. Cell-laden tissue-engineered skin substitutes have the ability to activate immune pathways, even in the absence of other immune-stimulating signals. In particular, mesenchymal stem cells with their immunomodulatory properties can create a specific immune microenvironment to reduce inflammation, scarring, and support skin regeneration. This review presents an overview of current wound care techniques including skin tissue engineering and biomaterials as a novel and promising approach. We highlight the plasticity and different roles of immune cells, in particular macrophages during various stages of skin wound healing. These aspects are pivotal to promote the regeneration of nonhealing wounds such as ulcers in diabetic patients. We believe that a better understanding of the intrinsic immunomodulatory features of stem cells in implantable skin substitutes will lead to new translational opportunities. This, in turn, will improve skin tissue engineering and regenerative medicine applications.

Preparation and properties of poly(ε-caprolactone)/bioactive glass nanofibre membranes for skin tissue engineering

2017 ◽  
Vol 33 (2) ◽  
pp. 195-209 ◽  
Author(s):  
Zefeng Lin ◽  
Wendong Gao ◽  
Limin Ma ◽  
Hong Xia ◽  
Weihan Xie ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bioactive Glass ◽  
Fibre Diameter ◽  
Skin Tissue ◽  
Glass Mass ◽  
Glass Composite ◽  
Elongation At Break ◽  
Skin Tissue Engineering ◽  
Electrospun Nanofibres

Poly(ε-caprolactone) composite nanofibres for skin tissue engineering and regeneration applications were prepared via electrospinning of poly(ε-caprolactone) nanofibres with bioactive glass nanoparticles at bioactive glass contents of 0, 2, 4, 6 and 8 wt%. The surface properties, water absorptivities, porosities, mechanical properties and biocompatibilities of the composite electrospun nanofibres were characterised in detail. Addition of bioactive glass improved the hydrophilicity and elastic modulus of membranes. The fibre diameter of the neat poly(ε-caprolactone) nanofibres was only 700 nm, but reinforcement with 2, 4, 6 and 8 wt% bioactive glass nanofibres increased the diameter to 1000, 1100, 900 and 800 nm, respectively. The minimum elongation at break of the bioactive glass–reinforced poly(ε-caprolactone) exceeded 100%, which indicated that the composite nanofibres had good mechanical properties. The porosities of the various nanofibres containing different mass loadings of bioactive glass all exceeded 90%. The best performance in terms of cell proliferation and adhesion was found when the bioactive glass mass percent reached 6 wt%. However, higher loadings were unfavourable for cell growth. These preliminary results indicate that poly(ε-caprolactone)/bioactive glass composite nanofibres have promise for skin tissue engineering applications.

scholarly journals Preliminary Characterization of a Polycaprolactone-SurgihoneyRO Electrospun Mesh for Skin Tissue Engineering

Materials ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 89
Author(s):  
Enes Aslan ◽  
Cian Vyas ◽  
Joel Yupanqui Mieles ◽  
Gavin Humphreys ◽  
Carl Diver ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Dermal Fibroblasts ◽  
Wound Dressings ◽  
Skin Tissue ◽  
Loss Of Function ◽  
Skin Substitutes ◽  
Disk Diffusion Test ◽  
Skin Tissue Engineering

Skin is a hierarchical and multi-cellular organ exposed to the external environment with a key protective and regulatory role. Wounds caused by disease and trauma can lead to a loss of function, which can be debilitating and even cause death. Accelerating the natural skin healing process and minimizing the risk of infection is a clinical challenge. Electrospinning is a key technology in the development of wound dressings and skin substitutes as it enables extracellular matrix-mimicking fibrous structures and delivery of bioactive materials. Honey is a promising biomaterial for use in skin tissue engineering applications and has antimicrobial properties and potential tissue regenerative properties. This preliminary study investigates a solution electrospun composite nanofibrous mesh based on polycaprolactone and a medical grade honey, SurgihoneyRO. The processing conditions were optimized and assessed by scanning electron microscopy to fabricate meshes with uniform fiber diameters and minimal presence of beads. The chemistry of the composite meshes was examined using Fourier transform infrared spectroscopy and X-ray photon spectroscopy showing incorporation of honey into the polymer matrix. Meshes incorporating honey had lower mechanical properties due to lower polymer content but were more hydrophilic, resulting in an increase in swelling and an accelerated degradation profile. The biocompatibility of the meshes was assessed using human dermal fibroblasts and adipose-derived stem cells, which showed comparable or higher cell metabolic activity and viability for SurgihoneyRO-containing meshes compared to polycaprolactone only meshes. The meshes showed no antibacterial properties in a disk diffusion test due to a lack of hydrogen peroxide production and release. The developed polycaprolactone-honey nanofibrous meshes have potential for use in skin applications.

Material properties in unconfined compression of gelatin hydrogel for skin tissue engineering applications

2014 ◽  
Vol 59 (6) ◽  
Author(s):  
Alireza Karimi ◽  
Mahdi Navidbakhsh
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Young’S Modulus ◽  
Maximum Stress ◽  
Young's Modulus ◽  
Material Model ◽  
Skin Tissue ◽  
Unconfined Compression ◽  
Engineering Applications ◽  
Skin Tissue Engineering

AbstractGelatin (Gel) has been reported as a promising candidate in tissue engineering owing to its easy availability, biocompatibility, and biodegradability. Gel hydrogel is of potential to be cross-linked with different materials to enhance their biocompatibility for cell culture for tissue engineering applications. The mechanical properties of this versatile material, however, have not been thoroughly determined. In this study, the linear elastic (Young’s modulus and maximum stress) and non-linear hyperelastic (hyperelastic coefficients) mechanical properties of prepared hydrogels at different contents of Gel (wt%) were measured, and its Young’s modulus was compared with that of skin tissue. The prepared cylindrical Gel hydrogels were subjected to a series of unconfined compression tests. The hyperelastic strain energy density function was calibrated using the compressive experimental data. The potential ability of the Yeoh hyperelastic constitutive equation, which has been proposed as the best material model to represent the non-linear behavior of hydrogels, was verified using finite element (FE) simulations. The results revealed that the Young’s modulus and maximum stress of hydrogels are increased by the addition of Gel. The highest Young’s modulus (81 kPa) and maximum stress (24 kPa) were observed for hydrogels with 15 wt% Gel. Results also showed that the hydrogels with a relatively lower content (<7.5 wt%) of Gel have suitable Young’s modulus compared with those with a higher content (>7.5 wt%) for skin tissue engineering. The Yeoh material model was closely fitted with the experimental data and could be used in further biomechanical simulations of the hydrogels. The experimental results were also compared well with those predicted by the FE models. The results of this study might have implications not only for the understanding of the mechanical properties of Gel hydrogel but also for the fabrication of polymeric substrate materials suitable for skin tissue engineering applications.

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