Ultra-high-density 3D DNA arrays within nanoporous biocompatible membranes for single-molecule-level detection and purification of circulating nucleic acids

Nanoscale ◽  
2015 ◽  
Vol 7 (14) ◽  
pp. 5998-6006 ◽  
Author(s):  
M. Aramesh ◽  
O. Shimoni ◽  
K. Fox ◽  
T. J. Karle ◽  
A. Lohrmann ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Single Molecule ◽  
High Density ◽  
Single Molecule Detection ◽  
Diamond Like Carbon ◽  
Dna Arrays ◽  
Single Molecule Level ◽  
Circulating Nucleic Acids ◽  
Carbon Coated ◽  
Oxide Membranes

Single-molecule-detection, selectivity, broad-range detection and biocompatibility are achieved using nanoporous diamond-like carbon coated oxide membranes.

scholarly journals Single-molecule detection of deoxyribonucleoside triphosphates in microdroplets

2019 ◽  
Vol 47 (17) ◽  
pp. e101-e101 ◽  
Author(s):  
Boris Breiner ◽  
Kerr Johnson ◽  
Magdalena Stolarek ◽  
Ana-Luisa Silva ◽  
Aurel Negrea ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Single Molecule ◽  
Dna Polymerases ◽  
Single Molecule Detection ◽  
Synthesis Methods ◽  
Single Nucleotide ◽  
New Approach ◽  
Fluorescent Signal ◽  
Single Molecule Level ◽  
Current Sequence

AbstractA new approach to single-molecule DNA sequencing in which dNTPs, released by pyrophosphorolysis from the strand to be sequenced, are captured in microdroplets and read directly could have substantial advantages over current sequence-by-synthesis methods; however, there is no existing method sensitive enough to detect a single nucleotide in a microdroplet. We have developed a method for dNTP detection based on an enzymatic two-stage reaction which produces a robust fluorescent signal that is easy to detect and process. By taking advantage of the inherent specificity of DNA polymerases and ligases, coupled with volume restriction in microdroplets, this method allows us to simultaneously detect the presence of and distinguish between, the four natural dNTPs at the single-molecule level, with negligible cross-talk.

scholarly journals Nucleic acid-based nanoengineering: novel structures for biomedical applications

2011 ◽  
Vol 1 (5) ◽  
pp. 702-724 ◽  
Author(s):  
Hanying Li ◽  
Thomas H. LaBean ◽  
Kam W. Leong
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Single Molecule ◽  
Biomedical Applications ◽  
Three Dimensional ◽  
Base Pairs ◽  
Single Molecule Level ◽  
Stimulus Responsive ◽  
Bioactive Agents ◽  
Novel Structures

Nanoengineering exploits the interactions of materials at the nanometre scale to create functional nanostructures. It relies on the precise organization of nanomaterials to achieve unique functionality. There are no interactions more elegant than those governing nucleic acids via Watson–Crick base-pairing rules. The infinite combinations of DNA/RNA base pairs and their remarkable molecular recognition capability can give rise to interesting nanostructures that are only limited by our imagination. Over the past years, creative assembly of nucleic acids has fashioned a plethora of two-dimensional and three-dimensional nanostructures with precisely controlled size, shape and spatial functionalization. These nanostructures have been precisely patterned with molecules, proteins and gold nanoparticles for the observation of chemical reactions at the single molecule level, activation of enzymatic cascade and novel modality of photonic detection, respectively. Recently, they have also been engineered to encapsulate and release bioactive agents in a stimulus-responsive manner for therapeutic applications. The future of nucleic acid-based nanoengineering is bright and exciting. In this review, we will discuss the strategies to control the assembly of nucleic acids and highlight the recent efforts to build functional nucleic acid nanodevices for nanomedicine.

scholarly journals Rolling circle amplification-driven encoding of different fluorescent molecules for simultaneous detection of multiple DNA repair enzymes at the single-molecule level

2020 ◽  
Vol 11 (22) ◽  
pp. 5724-5734
Author(s):  
Chen-chen Li ◽  
Hui-yan Chen ◽  
Juan Hu ◽  
Chun-yang Zhang
Keyword(s):  
Dna Repair ◽  
Single Molecule ◽  
Rolling Circle Amplification ◽  
Simultaneous Detection ◽  
Single Molecule Detection ◽  
Dna Repair Enzymes ◽  
Rolling Circle ◽  
Single Molecule Level ◽  
Repair Enzymes ◽  
Fluorescent Molecules

Integration of single-molecule detection with rolling circle amplification-driven encoding of different fluorescent molecules enables simultaneous detection of multiple DNA repair enzymes.

Antagonistic effects of natural osmolyte mixtures and hydrostatic pressure on the conformational dynamics of a DNA hairpin probed at the single-molecule level

2018 ◽  
Vol 20 (19) ◽  
pp. 13159-13170 ◽  
Author(s):  
Satyajit Patra ◽  
Christian Anders ◽  
Paul Hendrik Schummel ◽  
Roland Winter
Keyword(s):  
Hydrostatic Pressure ◽  
Nucleic Acids ◽  
Single Molecule ◽  
Deep Sea ◽  
Conformational Dynamics ◽  
Dna Hairpin ◽  
Antagonistic Effects ◽  
Single Molecule Level

Osmolyte mixtures from deep sea organisms are able to rescue nucleic acids from pressure-induced unfolding.

Comparative quantification of nucleic acids using single-molecule detection and molecular beacons

The Analyst ◽  
2005 ◽  
Vol 130 (4) ◽  
pp. 483 ◽  
Author(s):  
Chun-Yang Zhang ◽  
Shu-Yi Chao ◽  
Tza-Huei Wang
Keyword(s):  
Nucleic Acids ◽  
Single Molecule ◽  
Molecular Beacons ◽  
Single Molecule Detection

scholarly journals Decoding Circulating Nucleic Acids in Human Serum Using Microfluidic Single Molecule Spectroscopy

2010 ◽  
Vol 132 (16) ◽  
pp. 5793-5798 ◽  
Author(s):  
Kelvin J. Liu ◽  
Malcolm V. Brock ◽  
Ie-Ming Shih ◽  
Tza-Huei Wang
Keyword(s):  
Nucleic Acids ◽  
Human Serum ◽  
Single Molecule ◽  
Single Molecule Spectroscopy ◽  
Circulating Nucleic Acids

Allergen diagnosis microarray with high-density immobilization capacity using diamond-like carbon-coated chips for profiling allergen-specific IgE and other immunoglobulins

2011 ◽  
Vol 706 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Koichi Suzuki ◽  
Mineyoshi Hiyoshi ◽  
Hitomi Tada ◽  
Miwa Bando ◽  
Takao Ichioka ◽  
...  
Keyword(s):  
Specific Ige ◽  
High Density ◽  
Diamond Like Carbon ◽  
Carbon Coated

scholarly journals Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at single-molecule level

2021 ◽  
Author(s):  
Chen-Chen Li ◽  
Hui-yan Chen ◽  
Xiliang Luo ◽  
Juan Hu ◽  
Chun-yang Zhang
Keyword(s):  
Lung Cancer ◽  
Clinical Diagnosis ◽  
Single Molecule ◽  
Simultaneous Measurement ◽  
Signal Amplification ◽  
Simultaneous Detection ◽  
Single Molecule Detection ◽  
Single Molecule Level ◽  
Multicolor Fluorescence ◽  
Cancer Tissues

The simultaneous detection of multiple microRNAs (miRNAs) will facilitate the early clinical diagnosis. Herein, we demonstrate the integration of multicolor fluorophores-encoded cascade signal amplification with single-molecule detection for simultaneous measurement...

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