scholarly journals Rolling circle amplification-driven encoding of different fluorescent molecules for simultaneous detection of multiple DNA repair enzymes at the single-molecule level

2020 ◽  
Vol 11 (22) ◽  
pp. 5724-5734
Author(s):  
Chen-chen Li ◽  
Hui-yan Chen ◽  
Juan Hu ◽  
Chun-yang Zhang
Keyword(s):  
Dna Repair ◽  
Single Molecule ◽  
Rolling Circle Amplification ◽  
Simultaneous Detection ◽  
Single Molecule Detection ◽  
Dna Repair Enzymes ◽  
Rolling Circle ◽  
Single Molecule Level ◽  
Repair Enzymes ◽  
Fluorescent Molecules

Integration of single-molecule detection with rolling circle amplification-driven encoding of different fluorescent molecules enables simultaneous detection of multiple DNA repair enzymes.

scholarly journals Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization

2021 ◽  
Author(s):  
Dongjian Cao ◽  
Sa Wu ◽  
Caili Xi ◽  
Dong Li ◽  
Kaiheng Zhu ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescent Proteins ◽  
Signal To Noise Ratio ◽  
Rolling Circle Amplification ◽  
Simultaneous Detection ◽  
Single Strand ◽  
Detection Methods ◽  
Rolling Circle ◽  
High Level

Abstract Fluorescence in situ hybridization (FISH) is a powerful tool to visualize transcripts in fixed cells and tissues. Despite the recent advances in FISH detection methods, it remains challenging to achieve high-level FISH imaging with a simple workflow. Here, we introduce a method to prepare long single-strand DNA concatemers (lssDNAc) through a controllable rolling-circle amplification (CRCA). Prepared lssDNAcs were used to develop novel AmpFISH workflows. In addition, we present its applications in different scenarios. AmpFISH shows the following advantages: 1) enhanced FISH signal-to-noise ratio (SNR) up to 160-fold compared with single-molecule FISH; 2) simultaneous detection of FISH signals and fluorescent proteins or immunofluorescence (IF) in tissues; 3) simple workflows; and 4) cost-efficiency. In brief, AmpFISH provides convenient and versatile tools for sensitive RNA/DNA detection and to gain useful information on cellular molecules using simple workflows.

A plasmonic aptasensor for ultrasensitive detection of thrombin via arrested rolling circle amplification

2015 ◽  
Vol 51 (37) ◽  
pp. 7927-7930 ◽  
Author(s):  
Sai Wang ◽  
Sai Bi ◽  
Zonghua Wang ◽  
Jianfei Xia ◽  
Feifei Zhang ◽  
...  
Keyword(s):  
Single Molecule ◽  
Rolling Circle Amplification ◽  
Signal Generation ◽  
Ultrasensitive Detection ◽  
Eye Detection ◽  
Rolling Circle ◽  
Single Molecule Level ◽  
Nanoparticle Growth ◽  
Naked Eye ◽  
Naked Eye Detection

A sensitive signal generation mechanism for gold nanoparticle growth is applied in a plasmonic aptasensor, achieving naked-eye detection of thrombin at the single-molecule level.

scholarly journals Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at single-molecule level

2021 ◽  
Author(s):  
Chen-Chen Li ◽  
Hui-yan Chen ◽  
Xiliang Luo ◽  
Juan Hu ◽  
Chun-yang Zhang
Keyword(s):  
Lung Cancer ◽  
Clinical Diagnosis ◽  
Single Molecule ◽  
Simultaneous Measurement ◽  
Signal Amplification ◽  
Simultaneous Detection ◽  
Single Molecule Detection ◽  
Single Molecule Level ◽  
Multicolor Fluorescence ◽  
Cancer Tissues

The simultaneous detection of multiple microRNAs (miRNAs) will facilitate the early clinical diagnosis. Herein, we demonstrate the integration of multicolor fluorophores-encoded cascade signal amplification with single-molecule detection for simultaneous measurement...

scholarly journals DASH-type cryptochromes – solved and open questions

2020 ◽  
Vol 401 (12) ◽  
pp. 1487-1493
Author(s):  
Stephan Kiontke ◽  
Tanja Göbel ◽  
Annika Brych ◽  
Alfred Batschauer
Keyword(s):  
Dna Repair ◽  
Circadian Clock ◽  
Blue Light ◽  
Dna Repair Enzymes ◽  
Repair Enzymes ◽  
Open Questions ◽  
Essential Components ◽  
Repair Activity ◽  
Almost All ◽  
Uv Lesions

AbstractDrosophila, Arabidopsis, Synechocystis, human (DASH)-type cryptochromes (cry-DASHs) form one subclade of the cryptochrome/photolyase family (CPF). CPF members are flavoproteins that act as DNA-repair enzymes (DNA-photolyases), or as ultraviolet(UV)-A/blue light photoreceptors (cryptochromes). In mammals, cryptochromes are essential components of the circadian clock feed-back loop. Cry-DASHs are present in almost all major taxa and were initially considered as photoreceptors. Later studies demonstrated DNA-repair activity that was, however, restricted to UV-lesions in single-stranded DNA. Very recent studies, particularly on microbial organisms, substantiated photoreceptor functions of cry-DASHs suggesting that they could be transitions between photolyases and cryptochromes.

DNA Origami as a DNA Repair Nanosensor at the Single-Molecule Level

2013 ◽  
Vol 52 (30) ◽  
pp. 7747-7750 ◽  
Author(s):  
Maria Tintoré ◽  
Isaac Gállego ◽  
Brendan Manning ◽  
Ramon Eritja ◽  
Carme Fàbrega
Keyword(s):  
Dna Repair ◽  
Single Molecule ◽  
Dna Origami ◽  
Single Molecule Level

scholarly journals Highly specific imaging of mRNA in single cells by target RNA-initiated rolling circle amplification

2017 ◽  
Vol 8 (5) ◽  
pp. 3668-3675 ◽  
Author(s):  
Ruijie Deng ◽  
Kaixiang Zhang ◽  
Yupeng Sun ◽  
Xiaojun Ren ◽  
Jinghong Li
Keyword(s):  
Single Molecule ◽  
Single Cells ◽  
Rolling Circle Amplification ◽  
Robust Method ◽  
Single Nucleotide ◽  
Rolling Circle ◽  
Target Rna

We report a robust method for the efficient imaging of mRNA with single-nucleotide and near-single-molecule resolution in single cells.

Sign in / Sign up

Export Citation Format

Share Document