Determination of minimal sequence for binding of an aptamer. A comparison of truncation and hybridization inhibition methods

RSC Advances ◽  
2014 ◽  
Vol 4 (88) ◽  
pp. 47227-47233 ◽  
Author(s):  
Thao T. Le ◽  
Orada Chumphukam ◽  
Anthony E. G. Cass
Keyword(s):  
Thermodynamic Analysis ◽  
Minimal Sequence ◽  
Target Binding

A thermodynamic analysis of the effects of truncation or competitive hybridisation of an aptamer on target binding is presented.

Thermodynamic Analysis of Thermoelastic Martensitic Transformations

2004 ◽  
Vol 449-452 ◽  
pp. 1325-0 ◽  
Author(s):  
Yinong Liu
Keyword(s):  
Shape Memory Alloys ◽  
Shape Memory ◽  
Experimental Determination ◽  
Thermodynamic Parameters ◽  
Thermodynamic Analysis ◽  
Martensitic Transformations ◽  
Thermoelastic Martensitic Transformations ◽  
The Past ◽  
Concise Overview

This paper is concerned with the application of fundamental thermodynamic theories in the analysis of thermoelastic martensitic transformations in shape memory alloys, with a particular reference to polycrystalline NiTi. The discussion is delivered in two parts. The first part presents a concise overview of the fundamental theories of thermodynamics of thermoelastic martensitic transformations established in the past 30 years. The second part focuses on the principles governing the application of the theories, interpretation of the thermodynamic parameters defined in the theories, experimental determination of the parameters, and some common misperceptions and unjustified assumptions in practice concerning these parameters.

In Situ Mg2Si Reinforced Mg Alloy Synthesized in Mg-SiO2 System

2010 ◽  
Vol 146-147 ◽  
pp. 1775-1779
Author(s):  
Dan Huang ◽  
Yan Li Wang ◽  
Ying Wang ◽  
Hong Bao Cui ◽  
Xue Feng Guo
Keyword(s):  
Thermodynamic Analysis ◽  
Equilibrium Diagram ◽  
Mg Alloy ◽  
Experimental Temperature ◽  
Chinese Script ◽  
Solidification Condition ◽  
Sio2 System ◽  
Primary Mg2si

The thermodynamic analysis in Mg-SiO2 system was conducted for the determination of the priority reaction at experimental temperature. Then, Mg2Si reinforced Mg alloy was synthesized through reaction in Mg-SiO2 system at 730°C, whose Si mass fraction is up to approximately 10%. The thermodynamic analysis and microstructure investigation results show, that generation of reinforcing phase of Mg2Si in Mg alloy from the reaction in Mg-SiO2 system is feasible with lower free energy than that in Mg-Si at the same temperature; and under the experimental temperature and solidification condition, the microstructure of Mg-10Si alloy is composed of coarse primary Mg2Si dendrite crystal, Chinese script type eutectic Mg2Si, and α–Mg phase, the formation of α–Mg holes is resulted from the relatively high cooling rate leading to a deviation from the equilibrium diagram during solidification.

scholarly journals Thermodynamic analysis of combined cycle plant operation as part of an energy-saving system based on an absorption bromide-lithium refrigerating machine

2020 ◽  
Vol 4 (4) ◽  
pp. 57-65
Author(s):  
L. V. Galimova ◽  
◽  
D. Z. Bairamov ◽  
Keyword(s):  
Thermodynamic Analysis ◽  
Exergy Analysis ◽  
Exergy Efficiency ◽  
Combined Cycle ◽  
Plant Operation ◽  
Operating Modes ◽  
System A ◽  
Combined Cycle Plant ◽  
Current Energy

The main directions of research of the current energy-generating system, taking into account its technical limitations, are optimization and forecasting based on the analysis of its operating modes. Thermodynamic analysis involves determining the efficiency of the system based on the research of exergy efficiency and exergy losses. In this project, we propose methodic and results of exergy analysis of combined cycle gas plant operation as an object of energy production, the efficiency which is provided by cooling the outdoor air using an absorption bromide-lithium refrigerating machine. Conducting exergy analysis for determination of exergy destruction allow to determine the potential for increasing the efficiency of the system. A flow graph and an incident matrix are presented. The exergy efficiency of the combined cycle gas plant under the specified conditions is 46,5%. Based on the exergy analysis, the final diagram of the distribution of fluxes and losses of exergy of the combined cycle gas plant is presented

scholarly journals DETERMINATION OF REACTIVITY AND THERMODYNAMIC ANALYSIS OF NICKEL-BASED OXYGEN CARRIERS FOR CHEMICAL-LOOPING COMBUSTION

2019 ◽  
Vol 36 (3) ◽  
pp. 1155-1164
Author(s):  
Adolfo L. Figueredo ◽  
Fabíola C. Carvalho ◽  
José C. Nascimento ◽  
Juan A. C. Ruiz ◽  
Osvaldo Chiavone-Filho ◽  
...  
Keyword(s):  
Thermodynamic Analysis ◽  
Chemical Looping Combustion ◽  
Chemical Looping ◽  
Oxygen Carriers

scholarly journals A rapid bead-based radioligand binding assay for the determination of target-binding fraction and quality control of radiopharmaceuticals

2019 ◽  
Vol 71 ◽  
pp. 32-38 ◽  
Author(s):  
Sai Kiran Sharma ◽  
Serge K. Lyashchenko ◽  
Hijin A. Park ◽  
Nagavarakishore Pillarsetty ◽  
Yorann Roux ◽  
...  
Keyword(s):  
Quality Control ◽  
Binding Assay ◽  
Radioligand Binding ◽  
Radioligand Binding Assay ◽  
Target Binding

Identification and analysis of RNA editing events in ovarian serous cystadenoma using RNA-seq Data

2021 ◽  
Vol 21 ◽  
Author(s):  
Yulan Wang ◽  
Xiaofeng Song ◽  
Tianyi Xu
Keyword(s):  
Ovarian Cancer ◽  
Rna Editing ◽  
Molecular Mechanisms ◽  
Enrichment Analysis ◽  
Serous Cystadenoma ◽  
Pathway Enrichment Analysis ◽  
Normal Tissues ◽  
Coding Regions ◽  
Target Binding

Background: Recent studies have revealed thousands of A-to-I RNA editing events in primates. These events are closely related to the occurrence and development of multiple cancers, but the origination and general functions of these events in ovarian cancer remain incompletely understood. Objective: To further the determination of molecular mechanisms of ovarian cancer from the perspective of RNA editing. Methods : Here, we used the SNP-free RNA editing Identification Toolkit (SPRINT) to detect RNA editing sites. These editing sites were then annotated and related functional analysis was performed. Results: In this study, about 1.7 million RES were detected in each sample, and 98% of these sites were due to A-to-G editing and were mainly distributed in non-coding regions. More than 1,000 A-to-G RES were detected in CDS regions, and nearly 700 could lead to amino acid changes. Our results also showed that editing in the 3′UTR regions can influence miRNA-target binding. We predicted the network of changed miRNA-mRNA interaction caused by the A-to-I RNA editing sites. We also screened the differential RNA editing sites between ovarian cancer and adjacent normal tissues, and then performed GO and KEGG pathway enrichment analysis on the genes that contain these differential RNA editing sites. Finally, we identified the potential dysregulated RNA editing events in ovarian cancer samples. Conclusion: This study systematically identified and analyzed RNA editing events in ovarian cancer and laid a foundation to explore the regulatory mechanism of RNA editing and its function in ovarian cancer.

scholarly journals Determination of Characteristic Frequency for Identification of Hot Spots in Proteins

2012 ◽  
pp. 26-29
Author(s):  
Yashpal Yadav ◽  
Sulochana Wadhwani
Keyword(s):  
Hot Spots ◽  
Characteristic Frequency ◽  
Target Interaction ◽  
Protein Target ◽  
Resonant Recognition Model ◽  
Power Spectral ◽  
Alternative Approach ◽  
Target Binding ◽  
Consensus Spectrum

Identification of hot spots or protein-target binding sites in proteins using resonant recognition model requires the knowledge of characteristic frequency. For a successful protein target interaction, both the protein and the target signals must share the same characteristic frequency. The common characteristic frequency of a functional group of proteins is determined from the consensus spectrum obtained using DFT. In this work an alternative approach for identification of characteristic frequency using power spectral density is described. The performance of the proposed method is observed to be better than the DFT-based approach and is illustrated using simulation examples.

scholarly journals How Reliable Is the Electrochemical Readout of MIP Sensors?

Sensors ◽  
2020 ◽  
Vol 20 (9) ◽  
pp. 2677 ◽  
Author(s):  
Aysu Yarman ◽  
Frieder W. Scheller
Keyword(s):  
Specific Binding ◽  
In Situ Characterization ◽  
Faradaic Current ◽  
Diffusional Permeability ◽  
Redox Active ◽  
Nonspecific Interactions ◽  
Target Binding

Electrochemical methods offer the simple characterization of the synthesis of molecularly imprinted polymers (MIPs) and the readouts of target binding. The binding of electroinactive analytes can be detected indirectly by their modulating effect on the diffusional permeability of a redox marker through thin MIP films. However, this process generates an overall signal, which may include nonspecific interactions with the nonimprinted surface and adsorption at the electrode surface in addition to (specific) binding to the cavities. Redox-active low-molecular-weight targets and metalloproteins enable a more specific direct quantification of their binding to MIPs by measuring the faradaic current. The in situ characterization of enzymes, MIP-based mimics of redox enzymes or enzyme-labeled targets, is based on the indication of an electroactive product. This approach allows the determination of both the activity of the bio(mimetic) catalyst and of the substrate concentration.

Sign in / Sign up

Export Citation Format

Share Document