Precision improvement in dark-field microscopy imaging by using gold nanoparticles as an internal reference: a combined theoretical and experimental study

Nanoscale ◽  
2016 ◽  
Vol 8 (16) ◽  
pp. 8729-8736 ◽  
Author(s):  
Jun Ma ◽  
Yue Liu ◽  
Peng Fei Gao ◽  
Hong Yan Zou ◽  
Cheng Zhi Huang
Keyword(s):  
Experimental Study ◽  
Gold Nanoparticles ◽  
Measurement Errors ◽  
Dark Field ◽  
Internal Reference ◽  
Microscopy Imaging ◽  
Microscopic Imaging ◽  
Dark Field Microscopy

The internal reference (IR) was introduced to dark-field microscopic imaging (iDFM) technique to reduce the large unavoidable measurement errors, and improved precision of the acquired signals has been obtained.

A single gold nanoprobe for colorimetric detection of silver(i) ions with dark-field microscopy

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 2011-2016 ◽  
Author(s):  
Yi Fen Xie ◽  
Yun Ying Cheng ◽  
Meng Li Liu ◽  
Hong Yan Zou ◽  
Cheng Zhi Huang
Keyword(s):  
Signal Transduction ◽  
Gold Nanoparticles ◽  
Lake Water ◽  
Water Samples ◽  
Color Change ◽  
Colorimetric Detection ◽  
Dark Field ◽  
Microscopy Imaging ◽  
Dark Field Microscopy ◽  
Gold Nanoprobe

In this work, the formation of C–Ag+–C bonding between cytosines was utilized to induce interparticle coupling of gold nanoparticles modified with single-strand DNA, resulting in a color change as the signal transduction to quantify Ag+ sensitively under dark-field microscopy imaging, while we achieved the quantification of Ag+ could be directly realized in lake water samples and drug samples.

A galvanic exchange process visualized on single silver nanoparticles via dark-field microscopy imaging

Nanoscale ◽  
2018 ◽  
Vol 10 (26) ◽  
pp. 12805-12812 ◽  
Author(s):  
Jun Zhou ◽  
Tong Yang ◽  
Wei He ◽  
Zi Yu Pan ◽  
Cheng Zhi Huang
Keyword(s):  
Silver Nanoparticles ◽  
Light Scattering ◽  
Reaction Kinetics ◽  
Removal Rate ◽  
Exchange Process ◽  
Dark Field ◽  
Gold Deposition ◽  
Microscopy Imaging ◽  
Dark Field Microscopy ◽  
Kinetics Of

A galvanic exchange (GE) process was visually monitored in real-time using light scattering dark-field microscopy imaging, through which the reaction kinetics of the GE process was visualized and the silver removal rate and gold deposition rate on single nanoparticles were revealed.

scholarly journals Developing Hollow-Channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes

2018 ◽  
Vol 19 (8) ◽  
pp. 2327 ◽  
Author(s):  
Sunjie Ye ◽  
May Wheeler ◽  
James McLaughlan ◽  
Abiral Tamang ◽  
Christine Diggle ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Single Cell Analysis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Dark Field ◽  
Microscopy Imaging ◽  
Gold Nanoflowers ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Spherical Gold Nanoparticles ◽  
Low Cytotoxicity

Gold nanoparticles-enabled intracellular surface-enhanced Raman spectroscopy (SERS) provides a sensitive and promising technique for single cell analysis. Compared with spherical gold nanoparticles, gold nanoflowers, i.e., flower-shaped gold nanostructures, can produce a stronger SERS signal. Current exploration of gold nanoflowers for intracellular SERS has been considerably limited by the difficulties in preparation, as well as background signal and cytotoxicity arising from the surfactant capping layer. Recently, we have developed a facile and surfactant-free method for fabricating hollow-channel gold nanoflowers (HAuNFs) with great single-particle SERS activity. In this paper, we investigate the cellular uptake and cytotoxicity of our HAuNFs using a RAW 264.7 macrophage cell line, and have observed effective cellular internalization and low cytotoxicity. We have further engineered our HAuNFs into SERS-active tags, and demonstrated the functionality of the obtained tags as trimodal nanoprobes for dark-field and fluorescence microscopy imaging, together with intracellular SERS.

Real-time monitoring of oxidative etching on single Ag nanocubes via light-scattering dark-field microscopy imaging

Nanoscale ◽  
2015 ◽  
Vol 7 (37) ◽  
pp. 15209-15213 ◽  
Author(s):  
Yi Wang ◽  
Hong Yan Zou ◽  
Cheng Zhi Huang
Keyword(s):  
Light Scattering ◽  
Real Time ◽  
Dark Field ◽  
Real Time Monitoring ◽  
Microscopy Imaging ◽  
Real Time Mode ◽  
Oxidative Etching ◽  
Dark Field Microscopy

Oxidative etching on single Ag nanocubes was monitored in real-time mode through light-scattering dark-field microscopy imaging.

Analytical methods based on the light-scattering of plasmonic nanoparticles at the single particle level with dark-field microscopy imaging

The Analyst ◽  
2017 ◽  
Vol 142 (2) ◽  
pp. 248-256 ◽  
Author(s):  
Tian Li ◽  
Xi Wu ◽  
Feng Liu ◽  
Na Li
Keyword(s):  
Light Scattering ◽  
Single Particle ◽  
Analytical Methods ◽  
Imaging Technique ◽  
Dark Field ◽  
Plasmonic Nanoparticles ◽  
Microscopy Imaging ◽  
Single Particle Level ◽  
Dark Field Microscopy ◽  
Particle Level

This minireview summarizes analytical methods based on the light-scattering of gold nanoparticles with the dark-field microscopy imaging technique at the single particle level.

Colorimetric ultrasensitive detection of DNA based on the intensity of gold nanoparticles with dark-field microscopy

The Analyst ◽  
2018 ◽  
Vol 143 (17) ◽  
pp. 4051-4056 ◽  
Author(s):  
Jingjing Li ◽  
Caiyun Kong ◽  
Qingyun Liu ◽  
Zhengbo Chen
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Dark Field ◽  
Ultrasensitive Detection ◽  
Au Nps ◽  
Dark Field Microscopy ◽  
Nucleic Acid Assay

We present an ultrasensitive colorimetric nucleic acid assay based on the intensity of gold nanoparticles (Au NPs) using dark field microscopy.

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