A cell-penetrating protein designed for bimodal fluorescence and magnetic resonance imaging

A multifunctional targeted nanoplatform combining photothermal therapy and chemotherapy has emerged as a promising strategy for comprehensive therapies of breast cancer. In this study, we constructed human epidermal growth factor receptor 2 (Her2)-targeted gold nanoshelled poly(lactic- co-glycolic acid) hybrid nanocapsules encapsulating perfluorooctyl bromide, superparamagnetic iron oxide nanoparticles, and doxorubicin (Her2-GPDH nanocapsules) as theranostic agent for bimodal ultrasound/magnetic resonance imaging and synergistic photothermal-chemotherapy of Her2-postive breast cancer cells. Her2–GPDH nanocomposites possessed well-defined spherical morphology, and the average diameter was about 296 nm with good dispersion. Targeting assays demonstrated that Her2–GPDH nanocapsules exhibited higher targeting binding to Her2-positive SKBR3 cells than Her2-negative MDA-MB-231cells. The encapsulation efficiency and the loading content of doxorubicin in Her2–GPDH nanocapsules were 39 ± 1.45% and 3.8 ± 0.52%, respectively, and the agent exhibited pH-responsive and near-infrared light-triggered stepwise release behavior of doxorubicin. In vitro, the agent had potential to serve as feasible candidate for ultrasound imaging and T2-weighted magnetic resonance imaging with a relatively high relaxivity. Cell experiments confirmed that the agent had significant photothermal cytotoxicity on SKBR3 cells, and the combined photothermal–chemotherapy could significantly enhance the anti-tumor effect. In summary, the present Her2–GPDH nanocapsules, a novel multifunctional nanoplatform, will offer a new way for early bimodal molecular-level diagnosis and synergistic treatment of Her2-positve breast cancer.

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Red Fluorescent Emissive Gd-Phenolic Nanoparticles for In Vivo Fluorescence and Magnetic Resonance Bimodal Imaging

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3136 ◽

2021 ◽

Vol 17 (8) ◽

pp. 1635-1646

Author(s):

Weibing Xu ◽

Jia Zhang ◽

Minzhi Zhao ◽

Zhijie Yang ◽

Qingfeng Wu ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Metabolic Pathways ◽

Polymer Nanoparticles ◽

Resonance Imaging ◽

Dual Mode ◽

Bimodal Imaging ◽

Imaging Performance

Due to the combination of the high resolution of fluorescence imaging and the no limitation in penetration depth of magnetic resonance imaging, dual-mode imaging of magnetic resonance and fluorescence (MR/FI) have attracted extensive research in recent years. Herein, a novel MR/FI bimodal imaging probe is facile fabricated by attaching the rhodamine fluorophore covalently to the surface of the Gd-phenolic coordination polymer nanoparticles. The contents of Gd3+ and RB of the as prepared probe are calculated to be 8.2% and 12.5%. The quantum yield of the probe is about 8.84% as well as red fluorescent emissive. The longitudinal r1 value is 6.94 mM−1 s−1 and the ratio r2/r1 is very low and about 1.22. Subsequently, the and MR imaging and fluorescence both in vitro and In Vivo are performed. The metabolic pathways In Vivo are inferred by studying the bio-distribution of the probe in major organs. The as-prepared probe exhibits excellent imaging performance and biocompatibility, which is conducive to its further application.

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Multifunctional silica nanoparticles for optical and magnetic resonance imaging

Biological Chemistry ◽

10.1515/hsz-2012-0251 ◽

2013 ◽

Vol 394 (1) ◽

pp. 125-135 ◽

Cited By ~ 12

Author(s):

Rajendra Joshi ◽

Verena Feldmann ◽

Wolfgang Koestner ◽

Claudia Detje ◽

Sven Gottschalk ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Silica Nanoparticles ◽

Cell Penetrating Peptides ◽

The Body ◽

Complete Disappearance ◽

Resonance Imaging ◽

Cell Penetrating

Abstract The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9–16 μmol/g resulting in nanomaterials with high local longitudinal and transversal relaxivities (~1×105 and ~5×105 /mm/s/NP, respectively). Rapid cellular uptake was observed in vitro; however, larger extracellular agglomerates were also formed. In vivo administration revealed a fast distribution throughout the body followed by a nearly complete disappearance of fluorescence in all organs except the lungs, liver, and spleen after 24 h. Such NPs have the potential to serve as efficient multimodal probes in molecular imaging.

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Dependence of Blood Clot Lysis on the Mode of Transport of Urokinase into the Clot - A Magnetic Resonance Imaging Study in Vitro

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648188 ◽

1991 ◽

Vol 65 (05) ◽

pp. 549-552 ◽

Cited By ~ 55

Author(s):

A Blinc ◽

G Planinšič ◽

D Keber ◽

O Jarh ◽

G Lahajnar ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Pressure Difference ◽

Volume Flow Rate ◽

Blood Clot ◽

Linear Regression Analysis ◽

Imaging Study ◽

Clot Lysis ◽

Resonance Imaging

SummaryMagnetic resonance imaging was employed to study the dependence of clot lysing patterns on two different modes of transport of urokinase into whole blood clots. In one group of clots (nonperfused clots, n1 = 10), access of urokinase to the fibrin network was possible by diffusion only, whereas in the other group (perfused clots, n2 = 10) bulk flow of plasma containing urokinase was instituted through occlusive clots by a pressure difference of 3 .7 kPa (37 cm H2O) across 3 cm long clots with a diameter of 4 mm. It was determined separately that this pressure difference resulted in a volume flow rate of 5.05 ± 2.4 × 10−2 ml/min through occlusive clots. Perfused clots diminished in size significantly in comparison to nonperfused ones already after 20 min (p <0.005). Linear regression analysis of two-dimensional clot sizes measured by MRI showed that the rate of lysis was more than 50-times faster in the perfused group in comparison to the nonperfused group. It was concluded that penetration of the thrombolytic agent into clots by perfusion is much more effective than by diffusion. Our results might have some implications for understanding the differences in lysis of arterial and venous thrombi.

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