Spatiotemporal activation of molecules within cells using silica nanoparticles responsive to blue-green light

2015 ◽  
Vol 3 (37) ◽  
pp. 7427-7433 ◽  
Author(s):  
Takaki Amamoto ◽  
Tomoya Hirata ◽  
Hironori Takahashi ◽  
Mako Kamiya ◽  
Yasuteru Urano ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Green Light ◽  
Live Cells ◽  
Cargo Molecule

Vehicle-nanoparticles that release cargo molecule at the cytoplasm of live cells by blue-green light has been developed.

Green light emitting BSA conjugated dye supported silica nanoparticles for bio-imaging application.

2021 ◽  
Author(s):  
Sathya Ramalingam ◽  
Kalarical Janardhanan Sreeram ◽  
Raghava Rao Jonnalagadda
Keyword(s):  
Silica Nanoparticles ◽  
Targeted Delivery ◽  
Green Light ◽  
Poor Knowledge ◽  
Light Emitting ◽  
Imaging Application ◽  
Bio Imaging ◽  
Bio Compatibility

Silica nanoparticles has attracted a huge bio audience as a biosafe material for targeted delivery applications due to its ease of functionalisation, less toxicity, and bio-compatibility. However, poor knowledge of...

scholarly journals “Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 823 ◽  
Author(s):  
André Luiz Tessaro ◽  
Aurore Fraix ◽  
Ana Claudia Pedrozo da Silva ◽  
Elena Gazzano ◽  
Chiara Riganti ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Outer Part ◽  
Green Light ◽  
Biological Evaluation ◽  
Light Sources ◽  
Good Tolerability ◽  
Physiological Conditions ◽  
The Individual

This contribution reports the design, preparation, photophysical and photochemical characterization, as well as a preliminary biological evaluation of mesoporous silica nanoparticles (MSNs) covalently integrating a nitric oxide (NO) photodonor (NOPD) and a singlet oxygen (1O2) photosensitizer (PS) and encapsulating the anticancer doxorubicin (DOX) in a noncovalent fashion. These MSNs bind the NOPD mainly in their inner part and the PS in their outer part in order to judiciously exploit the different diffusion radius of the cytotoxic NO and 1O2. Furthermore this silica nanoconstruct has been devised in such a way to permit the selective excitation of the NOPD and the PS with light sources of different energy in the visible window. We demonstrate that the individual photochemical performances of the photoactive components of the MSNs are not mutually affected, and remain unaltered even in the presence of DOX. As a result, the complete nanoconstruct is able to deliver NO and 1O2 under blue and green light, respectively, and to release DOX under physiological conditions. Preliminary biological results performed using A375 cancer cells show a good tolerability of the functionalized MSNs in the dark and a potentiated activity of DOX upon irradiation, due to the effect of the NO photoreleased.

One-Pot Synthesis of Cy5-Encapsulated Photostable Fluorescent Silica Nanoparticles for Bioimaging

Nano LIFE ◽  
2015 ◽  
Vol 05 (03) ◽  
pp. 1540007 ◽  
Author(s):  
Yanjiao Lu ◽  
Bicheng He ◽  
Zhuo Gao ◽  
Jie Li ◽  
Jie Shen ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Water Soluble ◽  
Live Cells ◽  
Primary Amines ◽  
One Pot ◽  
Covalent Bonds ◽  
Fluorescent Silica Nanoparticles ◽  
Low Cytotoxicity ◽  
High Fluorescence ◽  
Microemulsion Synthesis

A new type of Cy5-encapsulated photostable fluorescent silica nanoparticles (FSNPs) bearing positive charges have been successfully fabricated by a reverse microemulsion synthesis in one-pot. The Cy5 dye containing four primary amines are embedded into silica via covalent bonds through a silane coupling agent (GPTMS), followed by co-condensation with tetraethylorthosilicate. The uniform-sized, spherical and monodispersed FSNPs have high fluorescence intensity and photostability. The FSNPs exhibit high stability, good biocompatibility as well as low cytotoxicity. These FSNPs can be internalized into live cells and thus fluorescently label the cells. This study provides a simple synthesis approach that can be applied to other water-soluble and amino-modified organic dye molecules for biological targeting and fluorescent cell imaging.

Acid and base dual-controlled cargo molecule release from polyaniline gated-hollow mesoporous silica nanoparticles

2016 ◽  
Vol 7 (42) ◽  
pp. 6467-6474 ◽  
Author(s):  
Xinyun Zhu ◽  
Jianliang Zhao ◽  
Caiqi Wang
Keyword(s):  
Controlled Release ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Acid And Base ◽  
Hollow Mesoporous Silica ◽  
Cargo Molecule

Polyaniline chains grafted onto the surface of hollow mesoporous silica served as the gate to realize drug dual-controlled release.

scholarly journals Silver Doped Mesoporous Silica Nanoparticles Based Electrochemical Enzyme-Less Sensor for Determination of H2O2 Released from Live Cells

Micromachines ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 268 ◽  
Author(s):  
Yang ◽  
Ni ◽  
Cao ◽  
Song ◽  
Alhamoud ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Electrochemical Sensor ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Fast Response ◽  
Live Cells ◽  
Glass Carbon Electrode ◽  
Long Term Stability ◽  
Glass Carbon

In this study, a silver doped mesoporous silica nanoparticles-based enzyme-less electrochemical sensor for the determination of hydrogen peroxide (H2O2) released from live cells was constructed for the first time. The presented electrochemical sensor exhibited fast response (2 s) towards the reduction of H2O2 concentration variation at an optimized potential of −0.5 V with high selectivity over biological interferents such as uric acid, ascorbic acid, and glucose. In addition, a wide linear range (4 μM to 10 mM) with a low detection limit (LOD) of 3 μM was obtained. Furthermore, the Ag-mSiO2 nanoparticles/glass carbon electrode (Ag-mSiO2 NPs/GCE) based enzyme-less sensor showed good electrocatalytic performance, as well as good reproducibility, and long-term stability, which provided a successful way to in situ determine H2O2 released from live cells. It may also be promising to monitor the effect of reactive oxygen species (ROS) production in bacteria against oxidants and antibiotics.

scholarly journals Correction: Acid and base dual-controlled cargo molecule release from polyaniline gated-hollow mesoporous silica nanoparticles

2016 ◽  
Vol 7 (48) ◽  
pp. 7530-7530
Author(s):  
Xinyun Zhu ◽  
Jianliang Zhao ◽  
Caiqi Wang
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Acid And Base ◽  
Hollow Mesoporous Silica ◽  
Cargo Molecule

Correction for ‘Acid and base dual-controlled cargo molecule release from polyaniline gated-hollow mesoporous silica nanoparticles’ by Xinyun Zhu, et al., Polym. Chem., 2016, 7, 6467–6474.

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

Green Light for ESRF Red Book

1989 ◽  
Vol 20 (2) ◽  
pp. 28-29
Keyword(s):  
Green Light
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