Purification, characterization, synthesis, in vivo and in vitro antihypertensive activity of bioactive peptides derived from coconut (Cocos nucifera L.) cake globulin hydrolysates

RSC Advances ◽  
2016 ◽  
Vol 6 (95) ◽  
pp. 92688-92698 ◽  
Author(s):  
Yan Li ◽  
Yajun Zheng ◽  
Yufeng Zhang ◽  
Liyun Liu ◽  
Songlin Zhao
Keyword(s):  
Bioactive Peptides ◽  
Cocos Nucifera ◽  
Antihypertensive Activity ◽  
Cocos Nucifera L

This paper reports the purification, characterization,in vivoandin vitroantihypertensive activity of two novel peptides derived from coconut cake globulin hydrolysates.

scholarly journals Comparative DNA Analysis of Coconut (Cocos nucifera L.) palms with polyembryonic and monoembryonic origins

2020 ◽  
pp. 21-29
Author(s):  
Daisy Jane Toting ◽  
Tessie Nuñez ◽  
Dilberto Ferraren
Keyword(s):  
Dna Analysis ◽  
Cocos Nucifera ◽  
Polyacrylamide Gel Electrophoresis ◽  
The Philippines ◽  
Pcr Products ◽  
Planting Materials ◽  
Cocos Nucifera L ◽  
Dna Primers

Makapuno is a rare, high-value coconut in the Philippines known for its extraordinary thick gelatinous meat with various uses in the food industry. Homozygous makapuno embryos do not germinate in vivo so plantlets are produced in vitro. where one plantlet grows from an embryo. Rare cases of polyembryony were observed in makapuno hybrids developed bythe Visayas State University, Knowledge of the genetic control of polyembryony may be used to increase the production of planting materials of these rare coconut types. DNA analysis of two sets of twins (polyembryonic), three monoembryonic hybrid palms, and their monoembryonic parental cultivars Coconiño and tall makapuno was done using seven DNA primers to determine differences which may be associated with polyembryony in the hybrids. Polyacrylamide Gel Electrophoresis of PCR products showed DNA fragments amplified by primers CAC2 and CAC56 which are unique to the twins suggesting that polyembryony might have a genetic origin.

In vitro and in vivo antihypertensive activity of palm kernel cake protein hydrolysates: Sequencing and characterization of potent bioactive peptides

2015 ◽  
Vol 76 ◽  
pp. 112-120 ◽  
Author(s):  
Mohammad Zarei ◽  
Bita Forghani ◽  
Afshin Ebrahimpour ◽  
Azizah Abdul-Hamid ◽  
Farooq Anwar ◽  
...  
Keyword(s):  
Bioactive Peptides ◽  
Palm Kernel ◽  
Protein Hydrolysates ◽  
Antihypertensive Activity ◽  
Palm Kernel Cake

The necessity of enzymatically hydrolyzed walnut protein to exert antihypertensive activity based on in vitro simulated digestion and in vivo verification

2021 ◽  
Author(s):  
Dandan Liu ◽  
Yiting Guo ◽  
Junsong Zhu ◽  
Weijie Tian ◽  
Min Chen ◽  
...  
Keyword(s):  
Enzymatic Degradation ◽  
Bioactive Peptides ◽  
Antihypertensive Activity ◽  
Simulated Digestion

Since not all proteins are suitable for preparing bioactive peptides by enzymatic degradation. The purpose of this study is to evaluate the necessity of walnut protein (WP) enzymolysis to exert...

Purification, characterization, synthesis, in vitro ACE inhibition and in vivo antihypertensive activity of bioactive peptides derived from oil palm kernel glutelin-2 hydrolysates

2017 ◽  
Vol 28 ◽  
pp. 48-58 ◽  
Author(s):  
Yajun Zheng ◽  
Yan Li ◽  
Youlin Zhang ◽  
Xiaohui Ruan ◽  
Runguang Zhang
Keyword(s):  
Oil Palm ◽  
Bioactive Peptides ◽  
Palm Kernel ◽  
Ace Inhibition ◽  
Antihypertensive Activity

Cardiovascular Effects of Micromeria graeca (L.) Benth. ex Rchb in Normotensive and Hypertensive Rats

2020 ◽  
Vol 20 (8) ◽  
pp. 1253-1261
Author(s):  
Mourad Akdad ◽  
Mohamed Eddouks
Keyword(s):  
Blood Pressure ◽  
Cardiovascular System ◽  
Arterial Blood Pressure ◽  
Antihypertensive Activity ◽  
Computer Assisted ◽  
Hypertensive Rats ◽  
Vasorelaxant Effect ◽  
Arterial Blood

Aims: The present study was performed in order to analyze the antihypertensive activity of Micromeria graeca (L.) Benth. ex Rchb. Background: Micromeria graeca (L.) Benth. ex Rchb is an aromatic and medicinal plant belonging to the Lamiaceae family. This herb is used to treat various pathologies such as cardiovascular disorders. Meanwhile, its pharmacological effects on the cardiovascular system have not been studied. Objective: The present study aimed to evaluate the effect of aqueous extract of aerial parts of Micromeria graeca (AEMG) on the cardiovascular system in normotensive and hypertensive rats. Methods: In this study, the cardiovascular effect of AEMG was evaluated using in vivo and in vitro investigations. In order to assess the acute effect of AEMG on the cardiovascular system, anesthetized L-NAME-hypertensive and normotensive rats received AEMG (100 mg/kg) orally and arterial blood pressure parameters were monitored during six hours. In the sub-chronic study, rats were orally treated for one week, followed by blood pressure assessment during one week of treatment. Blood pressure was measured using a tail-cuff and a computer-assisted monitoring device. In the second experiment, isolated rat aortic ring pre-contracted with Epinephrine (EP) or KCl was used to assess the vasorelaxant effect of AEMG. Results: Oral administration of AEMG (100 mg/kg) provoked a decrease of arterial blood pressure parameters in hypertensive rats. In addition, AEMG induced a vasorelaxant effect in thoracic aortic rings pre-contracted with EP (10 μM) or KCl (80 mM). This effect was attenuated in the presence of propranolol and methylene blue. While in the presence of glibenclamide, L-NAME, nifedipine or Indomethacin, the vasorelaxant effect was not affected. Conclusion: This study showed that Micromeria graeca possesses a potent antihypertensive effect and relaxes the vascular smooth muscle through β-adrenergic and cGMP pathways.

Vasorelaxant and Antihypertensive Effects of Mentha pulegium L. in Rats: An in vitro and in vivo Approach

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohammed Ajebli ◽  
Mohamed Eddouks
Keyword(s):  
Blood Pressure ◽  
Acute Experiment ◽  
Antihypertensive Activity ◽  
Mentha Pulegium ◽  
In Vitro Experiment ◽  
Arterial Blood ◽  
Dose Dependent ◽  
Ca2 Channels

Aims and objective: The aim of the study was to investigate the effect of aqueous aerial part extract of Mentha pulegium L. (Pennyrile) (MPAE) on arterial pressure parameters in rats. Background: Mentha pulegium is a medicinal plant used to treat hypertension in Morocco. Material and methods: In the current study, MPAE was prepared and its antihypertensive activity was pharmacologically investigated. L-NAME-hypertensive and normotensive rats have received orally MPAE (180 and 300 mg/kg) during six hours for the acute experiment and during seven days for the sub-chronic treatment. Thereafter, systolic, diastolic, mean arterial blood pressure and heart rate were evaluated. While, in the in vitro experiment, isolated denuded and intact thoracic aortic rings were suspended in a tissue bath system and the tension changes were recorded. Results: A fall in blood pressure was observed in L-NAME-induced hypertensive treated with MPAE. The extract also produced a dose-dependent relaxation of aorta pre-contracted with NE and KCl. The study showed that the vasorelaxant ability of MPAE seems to be exerted through the blockage of extracellular Ca2+ entry. Conclusion: The results demonstrate that the extract of pennyrile exhibits antihypertensive activity. In addition, the effect may be, at least in part, due to dilation of blood vessels via blockage of Ca2+ channels.

Flow cytometric analysis of the cell cycle in different coconut palm ( Cocos nucifera L.) tissues cultured in vitro

2003 ◽  
Vol 22 (1) ◽  
pp. 25-31 ◽  
Author(s):  
A. Sandoval ◽  
V. Hocher ◽  
J-L. Verdeil
Keyword(s):  
Cell Cycle ◽  
Cocos Nucifera ◽  
Flow Cytometric Analysis ◽  
Coconut Palm ◽  
Flow Cytometric ◽  
Cocos Nucifera L

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets

2005 ◽  
Vol 41 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Gabriela Fuentes ◽  
Carlos Talavera ◽  
Carlos Oropeza ◽  
Yves Desjardins ◽  
Jorge M. Santamaria
Keyword(s):  
Cocos Nucifera ◽  
In Vitro Plantlets ◽  
Survival And Growth ◽  
Cocos Nucifera L

Amaranth seed protein hydrolysates have in vivo and in vitro antihypertensive activity

2011 ◽  
Vol 126 (3) ◽  
pp. 878-884 ◽  
Author(s):  
Mariana Fritz ◽  
Bruno Vecchi ◽  
Gustavo Rinaldi ◽  
María Cristina Añón
Keyword(s):  
Seed Protein ◽  
Protein Hydrolysates ◽  
Antihypertensive Activity ◽  
Amaranth Seed
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