Bio-friendly Maillard reaction fluorescent products from glutathione and ascorbic acid for the rapid and label-free detection of Fe3+in living cells

2017 ◽  
Vol 5 (4) ◽  
pp. 707-713 ◽  
Author(s):  
Jiang Xue Dong ◽  
Zi Li Wang ◽  
Yue Yang ◽  
Zhong Feng Gao ◽  
Bang Lin Li ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Maillard Reaction ◽  
Living Cells ◽  
Label Free ◽  
Imaging Probe ◽  
Label Free Detection ◽  
Fluorescent Products

Non-cytotoxic Maillard reaction fluorescent products were used as an imaging probe for Fe3+detection in living cells.

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

scholarly journals Label-free detection of anticancer drug paclitaxel in living cells by confocal Raman microscopy

2013 ◽  
Vol 102 (11) ◽  
pp. 113701 ◽  
Author(s):  
H. Salehi ◽  
L. Derely ◽  
A.-G. Vegh ◽  
J.-C. Durand ◽  
C. Gergely ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Living Cells ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Label Free ◽  
Label Free Detection ◽  
Confocal Raman

Selective and high-sensitive label-free detection of ascorbic acid by carbon nitride quantum dots with intense fluorescence from lone pair states

Talanta ◽  
2019 ◽  
Vol 196 ◽  
pp. 530-536 ◽  
Author(s):  
Hangqing Xie ◽  
Yao Fu ◽  
Qizhen Zhang ◽  
Kun Yan ◽  
Run Yang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Ascorbic Acid ◽  
Carbon Nitride ◽  
Lone Pair ◽  
Label Free ◽  
Intense Fluorescence ◽  
Label Free Detection

scholarly journals Biomass-derived Carbon Quantum Dots for Visible-Light-Induced Photocatalysis and Label-Free Detection of Fe(III) and Ascorbic acid

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gouri Sankar Das ◽  
Jong Pil Shim ◽  
Amit Bhatnagar ◽  
Kumud Malika Tripathi ◽  
TaeYoung Kim
Keyword(s):  
Quantum Dots ◽  
Ascorbic Acid ◽  
Visible Light ◽  
Fluorescence Emission ◽  
Carbon Quantum Dots ◽  
Active Species ◽  
Label Free ◽  
One Pot ◽  
Label Free Detection ◽  
Visible Light Driven

Abstract Visible-light-driven photocatalysts prepared using renewable resources are crucial but challenging to develop for the efficient degradation of organic pollutants, which is required to solve ever-increasing water deterioration issues. In this study, we report a visible-light-responsive photocatalyst for the efficient degradation of methylene blue (MB) as a model pollutant dye. Green-emissive carbon quantum dots (CQDs) were synthesized from pear juice via a facile, scalable, one-pot solvothermal process. The as-synthesized CQDs exhibit superior photocatalytic activity under visible-light irradiation owing to their efficient light absorption, electron transfer, and separation of photogenerated charge carriers, facilitating ~99.5% degradation of MB within 130 min. A possible mechanism for the photocatalysis is proposed on the basis of comprehensive active species trapping experiments. Furthermore, the CQDs were used in a specific sensitive assay for Fe(III) and ascorbic acid (AA), even with interference from other metal ions. The fluorescence emission of CQDs was “turned off” specifically upon binding of Fe(III) and “turned on” with AA. The prepared CQDs represent efficient photocatalysts and fluorescent probes that are not restricted by toxicity, cost, or lack of scalability.

A potential fluorescent probe: Maillard reaction product from glutathione and ascorbic acid for rapid and label-free dual detection of Hg2+ and biothiols

2016 ◽  
Vol 81 ◽  
pp. 473-479 ◽  
Author(s):  
Jiang Xue Dong ◽  
Xiao Fang Song ◽  
Yan Shi ◽  
Zhong Feng Gao ◽  
Bang Lin Li ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Fluorescent Probe ◽  
Maillard Reaction ◽  
Label Free ◽  
Maillard Reaction Product ◽  
Dual Detection

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

Label-free proteomic analysis revealed the mechanisms of protein oxidation induced by hydroxyl radicals in whiteleg shrimp (Litopenaeus vannamei) muscle

2021 ◽  
Author(s):  
Hui-min Lin ◽  
Xue-er Qi ◽  
Shan-shan Shui ◽  
Soottawat Benjakul ◽  
Santiago P. Aubourg ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Hydroxyl Radicals ◽  
Litopenaeus Vannamei ◽  
Protein Oxidation ◽  
Proteomic Analysis ◽  
Muscle Proteins ◽  
Label Free ◽  
Whiteleg Shrimp ◽  
The Stability ◽  
Oxidative Effects

The oxidative effects of hydroxyl radicals derived from a FeCl3/ascorbic acid/H2O2 system on the stability of muscle proteins in peeled shrimp (Litopenaeus vannamei) were investigated.

scholarly journals Novel methods for the rapid detection of trace tetracyclines based on the fluorescence behaviours of Maillard reaction fluorescent nanoparticles

RSC Advances ◽  
2020 ◽  
Vol 10 (71) ◽  
pp. 43256-43261
Author(s):  
Xue-jing Si ◽  
Hong-ling Wang ◽  
Tun-hua Wu ◽  
Ping Wang
Keyword(s):  
Food Safety ◽  
Maillard Reaction ◽  
Rapid Detection ◽  
Fluorescent Nanoparticles ◽  
Fluorescent Products ◽  
Novel Methods

The Maillard reaction and its fluorescent products have attracted widespread attention in the field of food safety and biology.

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