Determination of free amino acids and nucleosides and nucleobases in Annona squamosa L. fruitages from different regions in China by LC-QTRAP-MS/MS

2017 ◽  
Vol 9 (25) ◽  
pp. 3862-3869 ◽  
Author(s):  
Ya-yun Chen ◽  
Peng Hu ◽  
Yuan-jiao Zhang ◽  
Yue Li ◽  
Xiao-li Zhu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Free Amino Acids ◽  
Free Amino ◽  
Quality Evaluation ◽  
Annona Squamosa

The quantitative analysis of amino acids, nucleosides and nucleobases is important for the quality evaluation of the popular fruit Annona squamosa L. obtained from different regions in China.

scholarly journals A METHOD FOR THE DETERMINATION OF FREE AMINO ACIDS IN RAT ORGANS AND TISSUES

1950 ◽  
Vol 182 (1) ◽  
pp. 29-37
Author(s):  
P.E. Schurr ◽  
H.T. Thompson ◽  
L.M. Henderson ◽  
C.A. Elvehjem
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Rat Organs

Chromatographic Separation of Free Amino Acids in Table Sirups

1971 ◽  
Vol 54 (1) ◽  
pp. 61-65
Author(s):  
Arthur Russell Johnson ◽  
Richard L Corliss ◽  
Enrique Fernandez-Flores
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Butyl Esters ◽  
Tlc Analysis

Abstract Qualitative chromatographic methods for the separation of free amino acids in table sirups are presented to aid in the development of chemical indices of composition which may be useful in establishing the identity of sirups and detecting their adulteration. Free amino acids in 2 table sirups were isolated on ion exchange columns and eluted with dilute ammonia. The concentrated amino acid mixture in the eluate was spotted directly on silica gel G plates for TLC analysis, or the amino acids were converted to their N-trifluoroacetyl n-butyl esters for GLC analysis. As many as 16 amino acids were qualitatively separated and identified and a potential for quantitative analysis was demonstrated.

Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Clinical Applications ◽  
Amino Groups ◽  
Automated Method ◽  
Total Free Amino Acids ◽  
Colored Complexes ◽  
Automated Determination

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

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