Determination of malachite green, crystal violet, brilliant green and methylene blue by micro-cloud-point extraction and nonlinear laser wave-mixing detection interfaced to micellar capillary electrophoresis

2017 ◽  
Vol 9 (45) ◽  
pp. 6411-6419 ◽  
Author(s):  
Jean Sebastien Pradel ◽  
William G. Tong
Keyword(s):  
Methylene Blue ◽  
Capillary Electrophoresis ◽  
Malachite Green ◽  
Crystal Violet ◽  
Brilliant Green ◽  
Cloud Point Extraction ◽  
Detection Method ◽  
Wave Mixing ◽  
Laser Wave

A laser wave-mixing CE detection method is reported for separation and detection of malachite green (MG), crystal violet (CV), brilliant green (BG), methylene blue (MB), and the leuco-metabolites of MG and CV residues in aquacultures.

Determination of Residues of Three Triphenylmethane Dyes and Their Metabolites (Malachite Green, Leuco Malachite Green, Crystal Violet, Leuco Crystal Violet, and Brilliant Green) in Aquaculture Products by LC/MS/MS: First Action 2012.25

2013 ◽  
Vol 96 (5) ◽  
pp. 1152-1157 ◽  
Author(s):  
Dominique Hurtaud-Pessel ◽  
Pierrick Couëdor ◽  
Eric Verdon ◽  
Dawn Dowell
Keyword(s):  
Malachite Green ◽  
Crystal Violet ◽  
Brilliant Green ◽  
Triphenylmethane Dyes ◽  
Method Performance ◽  
Green Crystal ◽  
Leuco Crystal Violet ◽  
Aquaculture Products ◽  
Leuco Malachite Green

Abstract During the AOAC Annual Meeting held from September 30 to October 3, 2012 in Las Vegas, NV, the Expert Review Panel (ERP) on Veterinary Drug Residues reviewed data for the method for determination of residues of three triphenylmethane dyes and their metabolites (malachite green, leuco malachite green, crystal violet, leuco crystal violet, and brilliant green) in aquaculture products by LC/MS/MS, previously published in the Journal of Chromatography A 1218, 1632–1645 (2006). The method data were reviewed and compared to the standard method performance requirements (SMPRs) found in SMPR 2009.001, published in AOAC's Official Methods of Analysis, 19th Ed. (2012). The ERP determined that the data were acceptable, and the method was approved AOAC Official First Action. The method uses acetonitrile to isolate the analyte from the matrix. Then determination is conducted by LC/MS/MS with positive electrospray ionization. Accuracy ranged from 100.1 to 109.8% for samples fortified at levels of 0.5, 0.75, 1.0, and 2.0 μg/kg. Precision ranged from 2.0 to 10.3% RSD for the intraday samples and 1.9 to 10.6% for the interday samples analyzed over 3 days. The described method is designed to accurately operate in the analytical range from 0.5 to 2 μg/kg, where the minimum required performance limit for laboratories has been fixed in the European Union at 2.0 μg/kg for these banned substances and their metabolites. Upper levels of concentrations (1–100 μg/kg) can be analyzed depending on the different optional calibrations used.

scholarly journals Determination of lethal concentration and antibacterial activity of commonly used disinfectants

1970 ◽  
Vol 1 (4) ◽  
pp. 102-105 ◽  
Author(s):  
M Alam ◽  
MM Rahman ◽  
MJ Foysal ◽  
MN Hossain
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Fluorescens ◽  
Potassium Permanganate ◽  
Malachite Green ◽  
Copper Sulfate ◽  
Pathogenic Bacteria ◽  
Labeo Rohita ◽  
Inhibitory Effect ◽  
Lethal Concentration

The toxic effects of four disinfectants viz., copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green on fish and fish pathogenic bacteria Aeromonas sp., Pseudomonas fluorescens, Edwardsiella sp. and Flavobacterium sp. were investigated. Lethal concentration of the disinfectants to fingerlings of Labeo rohita was determined in aquarium by standard method. Lethal concentration of copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green against fish were found in 0.75ppm, 7ppm, 6ppm and 0.5ppm at 21.4hrs, 18hrs, 9.5hrs and 1.40hrs, respectively. Methylene blue at 4ppm and 5ppm concentration inhibited the growth of Pseudomonas fluorescens and 6ppm concentration suppressed the growth of Aeromonas sp. Copper sulfate (CuSO4) was effective only against Edwardsiella sp at concentration of 10ppm and 8ppm. Malachite green repressed the growth of all four tasted bacteria at a concentration of 1ppm. Potassium permanganate (KMnO4) was failed to exhibit any inhibitory effect on the bacteria even at 30ppm concentration. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9738 IJNS 2011 1(4): 102-105

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