scholarly journals A material-basis study of Aloe vera on the wnt/β-catenin signaling pathway using a knockin/knockout method with high-speed countercurrent chromatography

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 38819-38829 ◽  
Author(s):  
Cong Dai ◽  
Meng-ping Liu ◽  
Wei-jia Zhang ◽  
Christopher Wai Kei Lam ◽  
Jian-ru Guo ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
High Speed ◽  
Target Genes ◽  
Aloe Vera ◽  
Water Extract ◽  
Hek293 Cells ◽  
Countercurrent Chromatography ◽  
Low Concentration ◽  
Material Basis

The water extract ofAloe veraat low concentration could activate the wnt/β-catenin signaling pathway and up-regulated the level of active β-catenin protein in hek293 cells, as well as promoting the expression of wnt target genes.

scholarly journals Pharmacological Evaluation of Artemisia cina Crude CO2 Subcritical Extract after the Removal of Santonin by Means of High Speed Countercurrent Chromatography

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2728 ◽  
Author(s):  
Zuriyadda Sakipova ◽  
Thais Biondino Sardella Giorno ◽  
Tolkyn Bekezhanova ◽  
Nikki Siu Hai Wong ◽  
Alma Shukirbekova ◽  
...  
Keyword(s):  
High Speed ◽  
Folk Medicine ◽  
Water Extract ◽  
Main Idea ◽  
Countercurrent Chromatography ◽  
Endemic Plant ◽  
Alcohol Extract ◽  
Economic Significance ◽  
Artemisia Species ◽  
Isolated Compound

Artemisia species are highly important due to their economic significance as medicines, fodder and food. Artemisia cina is an endemic species to Kazakhstan. In folk medicine, water extract of A. cina was used in the treatment of bronchial asthma while the alcohol extract has larvicidal and antituberculosis activity. The most common and most extensively studied compound from this species is the terpenoid santonin. The toxicity of this compound occurs at the doses of 60 mg for children and 200 mg for adults causing among other issues xanthopsia, leading to blindness. Having this in mind, the main idea of this work was to remove santonin from the crude extract and to check if the santonin-free extract would still be of any pharmacological importance. A CO2 subcritical extract was chromatographed using high-speed countercurrent chromatography (HSCCC) for the removal of santonin. The santonin-free CO2 subcritical extract (SFCO2E) as well as the isolated compound pectolinarigenin, a flavonoid, were assessed for their pharmacological actions. From the results obtained we can safely suggest that HSCCC is an efficient methodology to completely remove santonin from the CO2 subcritical extract. It was also possible to observe promising antinociceptive and anti–inflammatory activities for both SFCO2E and pectolinarigenin at concentrations that can justify the production of a phytomedicine with this endemic plant from Kazakhstan.

Separation of Aloins A and B from Aloe Vera Exudates by High Speed Countercurrent Chromatography

2007 ◽  
Vol 30 (11) ◽  
pp. 1657-1668 ◽  
Author(s):  
Xueli Cao ◽  
Danfeng Huang ◽  
Yinmao Dong ◽  
Hua Zhao ◽  
Yoichiro Ito
Keyword(s):  
High Speed ◽  
Aloe Vera ◽  
Countercurrent Chromatography

scholarly journals Study The Mechanism of Huangdi Anxiao Capsules In The Treatment of T2DM of Using UPLC-Q-TOF-MSE Combined With Network Pharmacological

2022 ◽  
Author(s):  
Wei Zhang ◽  
Li Shan ◽  
Meng Qing Zhu ◽  
Zhao Hui Fang ◽  
Xiao Chuang Liu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Target Genes ◽  
Target Prediction ◽  
Network Pharmacology ◽  
Rat Serum ◽  
Protein Protein Interaction ◽  
Material Basis ◽  
The Common ◽  
Tof Ms

Abstract This study was to explore the main material basis, target and pathway of Huangdi Anxiao capsule (HDAXC) for the treatment of type 2 diabetes mellitus (T2DM) by UPLC-Q-TOF-MSE and network pharmacology. In this study, HDAXC was administrated to T2DM rats, and its serum were detected by UPLC- Q-TOF-MSE, and the prototype components of HDAXC were analyzed. Using Swiss target prediction database to predict the target of serum prototype components, using GeneCards and DrugBack database to predict the target of T2DM. These common targets are the prediction target of HDAXC acting on T2DM. The key components of HDAXC in the treatment of T2DM were determined by using the software of Cytoscape3.7.2 to visualize the results. Using the STRING online platform to construct the protein-protein interaction (PPI), the key target genes were selected. The Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the common targets were carried out by using the OmicShare tools. And quantitative PCR and Western bolt were used to verify the related target genes. A toll of 28 prototype compounds were detected in rat serum, and 495 putative identified target genes were screened from HDAXC, of which 141 overlapped with the targets of T2DM and were considered potential therapeutic targets. The analysis of the network results showed that the key components of HDAXC are Magnoflorine, Galangin, Quercetin, and Epiberberine, etc. VEGFA, AKT1, SRC, EGFR might be the key target genes of HDAXC in the treatment of T2DM. HDAXC may have a therapeutic effect on T2DM by affecting HIF-1 signaling pathway, AGE-RAGE signaling pathway in diabetic complications, VEGFA signaling pathway and PI3K/AKT signaling pathways. In this study, compounds absorbed into the blood of HDAXC and its action target and pathway were preliminarily analyzed, which provided evidences for clarifying the chemical material basis and researching functional mechanism of HDAXC.

scholarly journals Qingyihuaji Formula Inhibits Pancreatic Cancer and Prolongs Survival by Downregulating Hes-1 and Hey-1

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Yanli Xu ◽  
Shan Xu ◽  
Yueqin Cai ◽  
Luming Liu
Keyword(s):  
Pancreatic Cancer ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Nude Mice ◽  
Target Genes ◽  
Advanced Pancreatic Cancer ◽  
Water Extract ◽  
Notch Signaling Pathway ◽  
Dependent Manner ◽  
Pancreatic Cancer Cells

The dire prognosis of pancreatic cancer has not markedly improved during past decades. The present study was carried out to explore the effect of Qingyihuaji formula (QYHJ) on inhibiting pancreatic cancer and prolonging survival in related Notch signaling pathway. Proliferation of pancreatic cancer cells (SW1990 and PANC-1) was detected by MTT assay at 24, 48, and 72 h with exposure to various concentrations (0.08–50 mg/mL) of QYHJ water extract. Pancreatic tumor models of nude mice were divided into three groups randomly (control, QYHJ, and gemcitabine). mRNA and protein expression of Notch target genes (Hes-1, Hey-1, Hey-2, and Hey-L) in dissected tumor tissue were detected. Results showed that proliferation of SW1990 cells and PANC-1 cells was inhibited by QYHJ water extract in a dose-dependent and time-dependent manner. QYHJ effectively inhibited tumor growth and prolonged survival time in nude mice. Expression of both Hes-1 and Hey-1 was decreased significantly in QYHJ group, suggesting that Hes-1 and Hey-1 in Notch signaling pathway might be potential targets for QYHJ treatment. This research could help explain the clinical effectiveness of QYHJ and may provide advanced pancreatic cancer patients with a new therapeutic option.

Enhancement of synergistic gene silencing by RNA interference using branched “3-in-1” trimer siRNA

2017 ◽  
Vol 5 (22) ◽  
pp. 4044-4051 ◽  
Author(s):  
Baiju G. Nair ◽  
Yue Zhou ◽  
Kyoji Hagiwara ◽  
Masashi Ueki ◽  
Takashi Isoshima ◽  
...  
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
High Activity ◽  
Signaling Pathway ◽  
Target Genes ◽  
Low Concentration ◽  
Gsk 3Β

Nanostructured RNA carrying three different siRNAs was assembled to silence three target genes (Axin, APC, and GSK-3β) in the Wnt/β-catenin signaling pathway. This nanostructured ‘3-in-1’ siRNA showed high activity at a low concentration due to the long-term resistance, and enhancing the effect of RNA interference.

Isolation of glucosinolates from broccoli seeds by use of high-speed countercurrent chromatography

2008 ◽  
Vol 20 (3) ◽  
pp. 489-496 ◽  
Author(s):  
Q. Du ◽  
L. Zhang ◽  
H. Cui ◽  
K. Wang ◽  
L. Xiu ◽  
...  
Keyword(s):  
High Speed ◽  
Countercurrent Chromatography

scholarly journals Mathematical analysis of the effect of portal vein cells on biliary epithelial cell differentiation through the Delta-Notch signaling pathway

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Masaharu Yoshihara ◽  
Teppei Nishino ◽  
Manoj Kumar Yadav ◽  
Akihiro Kuno ◽  
Takeshi Nagata ◽  
...  
Keyword(s):  
Portal Vein ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Target Genes ◽  
Notch Signaling Pathway ◽  
Epithelial Differentiation ◽  
Production Rates ◽  
Fine Grained ◽  
Downstream Target ◽  
Notch Receptors

Abstract Objective The Delta-Notch signaling pathway induces fine-grained patterns of differentiation from initially homogeneous progenitor cells in many biological contexts, including Drosophila bristle formation, where mathematical modeling reportedly suggests the importance of production rate of the components of this signaling pathway. In contrast, the epithelial differentiation of bile ducts in the developing liver is unique in that it occurs around the portal vein cells, which express extremely high amounts of Delta ligands and act as a disturbance for the amount of Delta ligands in the field by affecting the expression levels of downstream target genes in the cells nearby. In the present study, we mathematically examined the dynamics of the Delta-Notch signaling pathway components in disturbance-driven biliary differentiation, using the model for fine-grained patterns of differentiation. Results A portal vein cell induced a high Notch signal in its neighboring cells, which corresponded to epithelial differentiation, depending on the production rates of Delta ligands and Notch receptors. In addition, this epithelial differentiation tended to occur in conditions where fine-grained patterning was reported to be lacking. These results highlighted the potential importance of the stability towards homogeneity determined by the production rates in Delta ligands and Notch receptors, in a disturbance-dependent epithelial differentiation.

Separation of anti-ulcer flavonoids from Artemisia extracts by high-speed countercurrent chromatography

2011 ◽  
Vol 129 (2) ◽  
pp. 679-683 ◽  
Author(s):  
Kee Dong Yoon ◽  
Young-Won Chin ◽  
Min Hye Yang ◽  
Jinwoong Kim
Keyword(s):  
High Speed ◽  
Countercurrent Chromatography
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