Manufacture and characterization of anti-inflammatory liposomes from jumbo flying squid (Dosidicus gigas) skin phospholipid extraction

2018 ◽  
Vol 9 (7) ◽  
pp. 3986-3996 ◽  
Author(s):  
Yi-Feng Kao ◽  
Yi-Hsieng Samuel Wu ◽  
Chung-Hsi Chou ◽  
Shih-Guei Fu ◽  
Cheng-Wei Liu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dosidicus Gigas ◽  
Marine Phospholipids ◽  
Anti Inflammatory ◽  
Anti Inflammation ◽  
Jumbo Flying Squid

The anti-inflammation properties of marine phospholipids enriched with n-3 fatty acids contribute to anti-inflammatory and inflammation-resolving mediators.

scholarly journals Quantitative Profiling of Differentially Produced Oxylipins in Human Adipose Derived Mesenchymal Stem Cells Under Proinflammatory Stimulation

2021 ◽  
Author(s):  
Dan Wang ◽  
Zhengze Shen ◽  
Chaoqun Yu ◽  
Lifang Cheng ◽  
Daorong Chen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Immune Modulation ◽  
Lipid Mediator ◽  
Tnf Α ◽  
Regulation Function ◽  
The Impact ◽  
Anti Inflammation ◽  
Comprehensive Characterization ◽  
Quantitative Profiling

Abstract Background: MSCs have been proven to have immune modulation and anti-inflammation capabilities, but the mechanisms are still under investigation. Recently, oxylipins have been identified to be involved in the immuno-regulation function of MSCs. However, the impact of proinflammatory stimulation on the profiles of MSCs derived oxylipins has never been studied.Methods: In the present research, we employed a newly developed UPLC-MS/MS method to identify and quantify the oxylipin profile of ADSCs under proinflammatory stimulation (TNF-α and IFN-𝛾).Results: As a result, among the analyzed 71 oxylipins, we detected and quantified 49 oxylipins derived from six major n-6 and n-3 polyunsaturated fatty acids (PUFAs) including arachidonic (AA), linoleic (LA), alpha-linolenic (ALA), dihomo-𝛾-linolenic acid (DGLA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids in all samples, while 4 oxylipins were detected only in a part of samples and 18 were not detected in all samples. Nine oxylipins were found to be significantly differentially produced in ADSCs after 24 hours of inflammatory stimulation, among which, several oxylipins were reported to be having anti-inflammation or proresolving functions and involved in lipid mediator switching.Conclusions: The results reported here make a fundamental step towards a comprehensive characterization of MSCs derived oxylipins as potential modulators of inflammation and immunoreaction.

scholarly journals Human adipose derived mesenchymal stem cells differentially produced anti-inflammatory oxylipins under proinflammatory stimulation

2021 ◽  
Author(s):  
Dan Wang ◽  
Zhengze Shen ◽  
Chaoqun Yu ◽  
Lifang Cheng ◽  
Daorong Chen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Immune Modulation ◽  
Lipid Mediator ◽  
Tnf Α ◽  
Regulation Function ◽  
Anti Inflammation ◽  
Comprehensive Characterization

Abstract MSCs have been proven to have immune modulation and anti-inflammation capabilities, but the mechanisms are still under investigation. Recently, oxylipins have been identified to be involved in the immuno-regulation function of MSCs. In the present research, we employed a newly developed UPLC-MS/MS method to identify and quantify the oxylipin profile of ADSCs under proinflammatory stimulation (TNF-α and IFN-𝛾). As a result, among the analyzed 71 oxylipins, we detected and quantified 49 oxylipins derived from six major n-6 and n-3 polyunsaturated fatty acids (PUFAs) including arachidonic (AA), linoleic (LA), alpha-linolenic (ALA), dihomo-𝛾-linolenic acid (DGLA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids in all samples, while 4 oxylipins were detected only in a part of samples and 18 were not detected in all samples. Nine oxylipins were found to be significantly differentially produced in ADSCs after 24 hours of inflammatory stimulation, among which, several oxylipins were reported to be having anti-inflammation or proresolving functions and involved in lipid mediator switching. The results reported here make a fundamental step towards a comprehensive characterization of MSCs derived oxylipins as potential modulators of inflammation and immunoreaction.

Characterization of Haemanthus coccineus extract and its bioactive component: A promising and novel anti-inflammatory approach

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
S Fuchs ◽  
L Tzung-Harn Hsieh ◽  
W Saarberg ◽  
L Schaefer ◽  
CAJ Erdelmeier ◽  
...  
Keyword(s):  
Bioactive Component ◽  
Anti Inflammatory

Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Gamze Göger ◽  
Muhammed Allak ◽  
Ali Şen ◽  
Fatih Göger ◽  
Mehmet Tekin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Antimicrobial Activities ◽  
Antidiabetic Activity ◽  
Gram Negative Bacteria ◽  
Inhibitory Effects ◽  
Dicaffeoylquinic Acid ◽  
Anti Inflammatory ◽  
Phytochemical Profiles

Abstract Phytochemical profiles of essential oil (EO), fatty acids, and n-hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of Cota altissima L. J. Gay (syn. Anthemis altissima L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and β-caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and α-glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. CAM showed the highest antioxidant activity against DPPH and ABTS radicals with IC50 values of 126.60 and 144.40 μg/mL, respectively. In the anti-inflammatory activity, CAE demonstrated the highest antilipoxygenase activity with an IC50 value of 105.40 μg/mL, whereas, CAD showed the best inhibition of α-glucosidase with an IC50 value of 396.40 μg/mL in the antidiabetic activity. CAH was effective against Staphylococcus aureus at MIC = 312.5 µg/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of C. altissima.

scholarly journals Effects of ketogenic diet containing medium-chain fatty acids on serum inflammatory factor and mTOR signaling pathway in rats

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Huan Liu ◽  
Jingwei Huang ◽  
Hui Liu ◽  
Feng Li ◽  
Quansheng Peng ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Caloric Restriction ◽  
Ketogenic Diet ◽  
Mtor Pathway ◽  
Inflammatory Factor ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Sd Rats ◽  
Anti Inflammatory ◽  
Chain Fatty Acids

Abstract Background The ketogenic diet (KD) can promote the anti-inflammatory metabolic state and increase ketone body level in rats. This study was to explore the effects and differences of KD with or without medium-chain fatty acids (MCFAs) on serum inflammatory factors and mTOR pathway in Sprague–Dawley (SD) rats. Results Male SD rats were assigned to five groups: control diet (C), 20% caloric restriction diet (LC), 20% caloric restriction ketogenic diet (containing MCFAs) (LCKD1), 20% caloric restriction ketogenic diet (LCKD2) and 20% caloric restriction foreign ketogenic diet (LCKD3), and fed for 30 d. LC and KD could significantly reduce the body weight of rats; LC and KD containing MCFAs showed anti-inflammatory effects; KD without MCFAs decreased the concentration of mTOR1, while KD containing MCFAs decreased the expression of AMPK, mtor1 and P70sk. Conclusions KD containing MCFAs showed better effects on the mTOR pathway and anti-inflammation than that without MCFAs.

scholarly journals Polyphenolic Characterization of Merlot, Tannat and Syrah Skin Extracts at Different Degrees of Maturity and Anti-Inflammatory Potential in RAW 264.7 Cells

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 541
Author(s):  
Nawel Benbouguerra ◽  
Josep Valls-Fonayet ◽  
Stephanie Krisa ◽  
François Garcia ◽  
Cédric Saucier ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Ultra Performance Liquid Chromatography ◽  
Raw 264.7 Cells ◽  
Triple Quadrupole Mass Spectrometer ◽  
Polyphenolic Composition ◽  
Anti Inflammatory ◽  
Red Grape ◽  
Species Production ◽  
Grape Varieties

(1) Background: Both sensory quality and healthy attributes of Vitis vinifera grapes used for winemaking are closely related with the polyphenolic composition of their skins. (2) Methods: In this study, the polyphenolic characterization (flavan-3-ols, procyanidins, flavonols, stilbenes, anthocyanins) was investigated by ultra performance liquid chromatography coupled to a triple quadrupole mass spectrometer (UPLC-QqQ-MS). Skins from Vitis vinifera Merlot, Tannat, and Syrah red grape varieties cultivated in the south of France at different stages of ripening in 2018 were used. The anti-inflammatory and the antioxidant potential of the extracts were evaluated by the measure of nitric oxide (NO) and the intracellular reactive oxygen species production (ROS) in lipopolysaccharide (LPS)-stimulated macrophages. (3) Results: 41 polyphenols were quantified in all samples. Generally, the flavan-3-ol and procyanidin content decreased during ripening whereas the anthocyanins and stilbenes increased. In addition, as a novelty of this work, a wide identification and characterization of monomeric and oligomeric stilbenes was assessed by using authentic standards isolated in our laboratory, some of them (parthenocissin A and miyabenol C) reported for the first time in Merlot, Tannat and Syrah cultivars. The before-veraison skin extracts of all studied varieties, exhibited higher NO and ROS productions inhibition (>50%) proving both antioxidant and anti-inflammatory properties.

scholarly journals The Antimicrobial and Anti-Inflammatory Effects of Silver Nanoparticles Synthesised from Cotyledon orbiculata Aqueous Extract

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1343
Author(s):  
Caroline Tyavambiza ◽  
Abdulrahman Mohammed Elbagory ◽  
Abram Madimabe Madiehe ◽  
Mervin Meyer ◽  
Samantha Meyer
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Methicillin Resistance ◽  
Antimicrobial Activities ◽  
Transmission Electron ◽  
Anti Inflammatory ◽  
Anti Inflammation ◽  
Important Medicinal Plant ◽  
Pro Inflammatory Cytokines

Cotyledon orbiculata, commonly known as pig’s ear, is an important medicinal plant of South Africa. It is used in traditional medicine to treat many ailments, including skin eruptions, abscesses, inflammation, boils and acne. Many plants have been used to synthesize metallic nanoparticles, particularly silver nanoparticles (AgNPs). However, the synthesis of AgNPs from C. orbiculata has never been reported before. The aim of this study was to synthesize AgNPs using C. orbiculata and evaluate their antimicrobial and immunomodulatory properties. AgNPs were synthesized and characterized using Ultraviolet-Visible Spectroscopy (UV-Vis), Dynamic Light Scattering (DLS) and High-Resolution Transmission Electron Microscopy (HR-TEM). The antimicrobial activities of the nanoparticles against skin pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Methicillin Resistance Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans) as well as their effects on cytokine production in macrophages (differentiated from THP-1 cells) were evaluated. The AgNPs from C. orbiculata exhibited antimicrobial activity, with the highest activity observed against P. aeruginosa (5 µg/mL). The AgNPs also showed anti-inflammatory activity by inhibiting the secretion of pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-1 beta) in lipopolysaccharide-treated macrophages. This concludes that the AgNPs produced from C. orbiculata possess antimicrobial and anti-inflammation properties.

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