scholarly journals The antifungal activity of extracts of Osmundea pinnatifida, an edible seaweed, indicates its usage as a safe environmental fungicide or as a food additive preventing post-harvest fungal food contamination

2018 ◽  
Vol 9 (12) ◽  
pp. 6187-6195 ◽  
Author(s):  
Paulo Silva ◽  
Chantal Fernandes ◽  
Lillian Barros ◽  
Isabel C. F. R. Ferreira ◽  
Leonel Pereira ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Food Contamination ◽  
Food Additive ◽  
Edible Seaweed ◽  
Post Harvest ◽  
Alternaria Infectoria

Extracts of the edible seaweed Osmundea pinnatifida impair Aspergillus fumigatus and Alternaria infectoria conidiation.

scholarly journals Lecythomycin, a New Macrolactone Glycoside from the Endophytic Fungus Lecythophora sp

2011 ◽  
Vol 6 (5) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Noor Erma Sugijanto ◽  
Arnulf Diesel ◽  
Mostafa Rateb ◽  
Alexander Pretsch ◽  
Selma Gogalic ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Endophytic Fungus ◽  
Mass Spectrometric ◽  
Mass Spectrometric Data ◽  
Minimal Inhibitory Concentrations ◽  
Spectrometric Data ◽  
Isolated Compound

A new macrolactone glycoside, lecythomycin (1), 23-methyl-3-(1- O-mannosyl)-oxacyclotetracosan-1-one, was isolated from the endophytic fungus Lecythophora sp. (code 30.1), an endopyte of the Indonesian plant Alyxia reinwardtii. The structure of 1 was elucidated on the basis of NMR spectroscopic and mass spectrometric data. The isolated compound displayed antifungal activity against strains of Aspergillus fumigatus and Candida kruzei at minimal inhibitory concentrations (MIC) of 62.5 – 125 μg/mL.

scholarly journals In VitroInteractions of Antifungal Agents and Tacrolimus against Aspergillus Biofilms

2015 ◽  
Vol 59 (11) ◽  
pp. 7097-7099 ◽  
Author(s):  
Lujuan Gao ◽  
Yi Sun
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Amphotericin B ◽  
Aspergillus Flavus ◽  
Inhibitory Activity ◽  
Aspergillus Terreus ◽  
Antifungal Agents ◽  
Broth Microdilution ◽  
Content Type ◽  
Antagonistic Effects

ABSTRACTAspergillusbiofilms were prepared fromAspergillus fumigatus,Aspergillus flavus, andAspergillus terreusvia a 96-well plate-based method, and the combined antifungal activity of tacrolimus with azoles or amphotericin B againstAspergillusbiofilms was investigated via a broth microdilution checkerboard technique system. Our results suggest that combinations of tacrolimus with voriconazole or amphotericin B have synergistic inhibitory activity againstAspergillusbiofilms. However, combinations of tacrolimus with itraconazole or posaconazole exhibit no synergistic or antagonistic effects.

Culture condition-dependent metabolite profiling of Aspergillus fumigatus with antifungal activity

2013 ◽  
Vol 117 (3) ◽  
pp. 211-219 ◽  
Author(s):  
Daejung Kang ◽  
Gun Hee Son ◽  
Hye Min Park ◽  
Jiyoung Kim ◽  
Jung Nam Choi ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Culture Condition ◽  
Metabolite Profiling

scholarly journals Chemoenzymatic Synthesis, Nanotization, and Anti-Aspergillus Activity of Optically Enriched Fluconazole Analogues

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Shashwat Malhotra ◽  
Seema Singh ◽  
Neha Rana ◽  
Shilpi Tomar ◽  
Priyanka Bhatnagar ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Chemoenzymatic Synthesis ◽  
The Novel ◽  
Disc Diffusion ◽  
Broth Microdilution ◽  
Content Type ◽  
Enantiomerically Pure ◽  
Activity Spectrum ◽  
Reduced Toxicity

ABSTRACT Despite recent advances in diagnostic and therapeutic methods in antifungal research, aspergillosis still remains a leading cause of morbidity and mortality. One strategy to address this problem is to enhance the activity spectrum of known antifungals, and we now report the first successful application of Candida antarctica lipase (CAL) for the preparation of optically enriched fluconazole analogues. Anti-Aspergillus activity was observed for an optically enriched derivative, (−)-S-2-(2′,4′-difluorophenyl)-1-hexyl-amino-3-(1‴,2‴,4‴)triazol-1‴-yl-propan-2-ol, which exhibits MIC values of 15.6 μg/ml and 7.8 μg/disc in broth microdilution and disc diffusion assays, respectively. This compound is tolerated by mammalian erythrocytes and cell lines (A549 and U87) at concentrations of up to 1,000 μg/ml. When incorporated into dextran nanoparticles, the novel, optically enriched fluconazole analogue exhibited improved antifungal activity against Aspergillus fumigatus (MIC, 1.63 μg/ml). These results not only demonstrate the ability of biocatalytic approaches to yield novel, optically enriched fluconazole derivatives but also suggest that enantiomerically pure fluconazole derivatives, and their nanotized counterparts, exhibiting anti-Aspergillus activity may have reduced toxicity.

Heat-Treated Fungizone Retains Amphotericin B Antifungal Activity Without Renal Toxicity in Rats Infected with Aspergillus Fumigatus

2004 ◽  
Vol 21 (9) ◽  
pp. 1564-1566 ◽  
Author(s):  
Olena Sivak ◽  
Karen Bartlett ◽  
Kishor M. Wasan
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Amphotericin B ◽  
Renal Toxicity ◽  
Heat Treated

scholarly journals Phytochemical screening and antifungal potency of Vernonia amygdalina (bitter leaf) extract against post harvest mycodeterioration of tomato (Lycopersicum esculentum)

2020 ◽  
Vol 7 (17) ◽  
pp. 327-337
Author(s):  
U. N. Emiri ◽  
E. B. Enaregha
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Diffusion Method ◽  
Lycopersicum Esculentum ◽  
Phytochemical Screening ◽  
Rhizopus Stolonifer ◽  
Vernonia Amygdalina ◽  
Tomato Fruits ◽  
Post Harvest ◽  
Ethanolic Extracts

The study investigated fungi associated with post harvest tomato fruits sold in the open market in Port Harcourt Metropolis. The antifungal activity (bitter leaf) extracts against spoilage was studied using well-in-agar diffusion method. Results showed that mean percentage incidence of fungi isolated from tomato fruits were Rhizopus stolonifer (56%), Aspergillus niger (62%) and Altermaria altermata (35%). Aqueous and ethanolic extracts of Vernonia amygdalina at different concentrations (20%, 40%, and 60%) (w/v) were used against fungi isolates, while water and ketoconzole (0.5 mg/mL) served as negative and positive control, respectively. Aqueous and ethanolic extracts of V. amygdalina inhibited the growth of all three test fungi. There were significant differences (p > 0.05) in the mean inhibitory effects of plant extracts and control. Antifungal activity measured as diameter of zone of inhibition revealed that V. amygdalina acqueous extract at 60% was very active against Aspergillus niger (16.50 mm) and Alternaria altermata (16.00 mm), while being moderately active against Rhizopus stolonifer (13.00 mm). However, 60% ethanolic extract of V. amygdalina was very active against A. niger (19.00 mm), A. altermata (17.00 mm) and Rhizopus stolonifer (15.80 mm). Phytochemical screening of V. amygdalina revealed the presence of tannins, oxalate, saponnins, flavanoid, cynogenic glucoside, phytate and Alkaloids V. amygdalina could serve as a potentially viable alternative to chemical fungicides in the preservation of post harvest tomato fruits (Lycopersicum esculentum).

scholarly journals Antifungal properties of volatile organic compounds produced by Daldinia eschscholtzii MFLUCC 19-0493 isolated from Barleria prionitis leaves against Colletotrichum acutatum and its post-harvest infections on strawberry fruits

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11242
Author(s):  
Sarunpron Khruengsai ◽  
Patcharee Pripdeevech ◽  
Chutima Tanapichatsakul ◽  
Chanin Srisuwannapa ◽  
Priya Esilda D’Souza ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Endophytic Fungi ◽  
Colletotrichum Acutatum ◽  
Anthracnose Disease ◽  
Post Harvest ◽  
Volatile Organic

Fungal endophytes are microorganisms living symbiotically with a host plant. They can produce volatile organic compounds (VOCs) that have antimicrobial activity. This study aimed to isolate endophytic fungi from Barleria prionitis plants grown in Thailand and to investigate the antifungal properties of their VOCs against Colletotrichum acutatum, a causal agent of anthracnose disease on post-harvest strawberry fruits. A total of 34 endophytic fungi were isolated from leaves of B. prionitis. The VOCs produced from each individual isolate were screened for their antifungal activity against C. acutatum using a dual-culture plate method. From this in vitro screening experiment, the VOCs produced by the endophytic isolate BP11 were found to have the highest inhibition percentage (80.3%) against the mycelial growth of C. acutatum. The endophytic isolate BP11 was molecularly identified as Daldinia eschscholtzii MFLUCC 19-0493. This strain was then selected for an in vivo experiment. Results from the in vivo experiment indicated that the VOCs produced by D. eschscholtzii MFLUCC 19-0493 were able to inhibit infections by C. acutatum on organic fresh strawberry fruits with an average inhibition percentage of 72.4%. The quality of the pathogen-inoculated strawberry fruits treated with VOCs produced by D. eschscholtzii MFLUCC 19-0493 was evaluated. Their fruit firmness, total soluble solids, and pH were found to be similar to the untreated strawberry fruits. Solid phase microextraction-gas chromatographic-mass spectrometric analysis of the VOCs produced by D. eschscholtzii MFLUCC 19-0493 led to the detection and identification of 60 compounds. The major compounds were elemicin (23.8%), benzaldehyde dimethyl acetal (8.5%), ethyl sorbate (6.8%), methyl geranate (6.5%), trans-sabinene hydrate (5.4%), and 3,5-dimethyl-4-heptanone (5.1%). Each major compound was tested for its antifungal activity against C. acutatum using the in vitro assay. While all these selected VOCs showed varying degrees of antifungal activity, elemicin was found to possess the strongest antifungal activity. This work suggests that D. eschscholtzii MFLUCC 19-0493 could be a promising natural preservative for controlling C. acutatum associated anthracnose disease in strawberry fruits during the post-harvest period.

scholarly journals Biocontrol Ability and Mechanism of a Broad-Spectrum Antifungal Strain Bacillus safensis sp. QN1NO-4 Against Strawberry Anthracnose Caused by Colletotrichum fragariae

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaojuan Li ◽  
Miaoyi Zhang ◽  
Dengfeng Qi ◽  
Dengbo Zhou ◽  
Chunlin Qi ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Broad Spectrum ◽  
Gene Clusters ◽  
Morinda Citrifolia ◽  
Gas Chromatography Mass Spectrometry ◽  
Post Harvest ◽  
Bacillus Safensis ◽  
Strawberry Anthracnose ◽  
Colletotrichum Fragariae

Strawberry is a very popular fruit with a special taste, color, and nutritional value. Anthracnose caused by Colletotrichum fragariae severely limits fruit shelf life during post-harvest storage. Use of traditional chemical fungicides leads to serious environment pollution and threatens food safety. Biocontrol is considered as a promising strategy to manage the post-harvest fruit diseases. Here, strain QN1NO-4 isolated from noni (Morinda citrifolia L.) fruit exhibited a high antifungal activity against C. fragariae. Based on its physicochemical profiles and phylogenetic tree of the 16S rRNA sequence, strain QN1NO-4 belonged to the genus Bacillus. The average nucleotide identity (ANI) calculated by comparing two standard strain genomes was below 95–96%, suggesting that the strain might be a novel species of the genus Bacillus and named as Bacillus safensis sp. QN1NO-4. Its extract effectively reduced the incidence of strawberry anthracnose of harvested fruit. Fruit weight and TSS contents were also maintained significantly. The antifungal mechanism assays indicated that the extract of the test antagonist inhibited mycelial growth and spore germination of C. fragariae in vitro. Cells of strain QN1NO-4 demonstrated the cytoplasmic heterogeneity, disappeared organelles, and ruptured ultrastructure. Notably, the strain extract also had a broad-spectrum antifungal activity. Compared with the whole genome of strain QN1NO-4, several functional gene clusters involved in the biosynthesis of active secondary metabolites were observed. Fifteen compounds were identified by gas chromatography–mass spectrometry (GC-MS). Hence, the fruit endophyte B. safensis sp. QN1NO-4 is a potential bio-agent identified for the management of post-harvest disease of strawberry fruit.

scholarly journals TINOSPORA CORDIFOLIA AQUEOUS EXTRACT AMELIORATES THE SYSTEMIC INFECTION OF ASPERGILLUS FUMIGATUS IN BALB/C MICE

2019 ◽  
pp. 525-528 ◽  
Author(s):  
MASOOD A KHAN
Keyword(s):  
Survival Rate ◽  
Antifungal Activity ◽  
Aspergillus Fumigatus ◽  
Aqueous Extract ◽  
Survival Data ◽  
Systemic Infection ◽  
Tinospora Cordifolia ◽  
Fungal Burden

Objective: The present study was aimed to assess the antifungal activity of Tinospora cordifolia aqueous extract (TCAE) against Aspergillus fumigatus infection. Methods: TCAE was tested for in vitro antifungal activity against the isolates of A. fumigatus, Aspergillus flavus, and Aspergillus niger. To evaluate in vivo activity, various doses (10, 25, and 50 mg/kg) of TCAE were orally administered in A. fumigatus-infected mice for 7 days. The combination of prophylactic and therapeutic effect of TCAE was assessed by pre-treating the mice with 10 mg/kg of TCAE for 3 consecutive days before exposing them to A. fumigatus. Mice were treated with 10, 25, and 50 mg/kg doses of TCAE for 7 consecutive days’ post-A. fumigatus infection. The effectiveness of TCAE was evaluated by monitoring the survival rate and assessing the fungal burden in the kidney of the treated mice. Results: A. fumigatus-infected mice treated with TCAE at the doses of 25 and 50 mg/kg exhibited 50% and 20% survival rate, respectively, observed on day 40 post-treatment. Like to the survival data, the fungal burden was also found to be the lowest in the kidney of mice treated with TCAE at a dose of 50 mg/kg. The results showed that pre-treatment with TCAE (10 mg/kg) followed by post-infection treatment with 10, 25, and 50 mg/kg of TCAE for 7 days resulted in 40%, 50%, and 70% survival rate, respectively. Conclusions: These results suggest that TCAE may potentially be considered for its possible use in the treatment of the systemic infection of A. fumigatus.

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