Metal oxide nanoparticle-functionalized sebacic acid-grafted PHEAM nanocarriers for enriched activity of metronidazole against food borne bacteria: in vitro and in vivo study

2018 ◽  
Vol 42 (22) ◽  
pp. 18437-18447 ◽  
Author(s):  
Murugesan Gowri ◽  
Kannan Suganya ◽  
Nachimuthu Latha ◽  
Marudhamuthu Murugan ◽  
Mukhtar Ahmed ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Metal Oxide ◽  
Sebacic Acid ◽  
In Vivo Study ◽  
Metal Oxide Nanoparticle ◽  
Food Borne ◽  
Oxide Nanoparticle

Food borne infection is a serious complication caused by Listeria monocytogenes (L. monocytogenes), a dangerous bacteria.

scholarly journals The Listeria monocytogenes Virulence Factor InlJ Is Specifically Expressed In Vivo and Behaves as an Adhesin

2008 ◽  
Vol 76 (4) ◽  
pp. 1368-1378 ◽  
Author(s):  
Christophe Sabet ◽  
Alejandro Toledo-Arana ◽  
Nicolas Personnic ◽  
Marc Lecuit ◽  
Sarah Dubrac ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Cultured Cells ◽  
Brain Heart Infusion ◽  
Gene Encoding ◽  
Regulatory Pathways ◽  
Food Borne ◽  
Food Body ◽  
Brain Heart Infusion Medium

ABSTRACT The food-borne pathogen Listeria monocytogenes is adapted to a diversity of environments, such as soil, food, body fluids, and the cytosol of eukaryotic cells. The transition between saprophytic and pathogenic life is mediated through complex regulatory pathways that modulate the expression of virulence factors. Here we examined the expression of inlJ, a recently identified gene encoding a protein of the LPXTG-internalin family and involved in pathogenesis. We show that inlJ expression is controlled neither by the major listerial regulator of virulence genes, PrfA, nor by AxyR, a putative AraC regulator encoded by a gene adjacent to inlJ and divergently transcribed. The InlJ protein is not produced by bacteria grown in vitro in brain heart infusion medium or replicating in the cytosol of tissue-cultured cells. In contrast, it is efficiently produced and localized at the surface of bacteria present in the liver and blood of infected animals. Strikingly, the expression of inlJ by a heterologous promoter in L. monocytogenes or L. innocua promotes bacterial adherence to human cells in vitro. Taken together, these results strongly suggest that InlJ acts as a novel L. monocytogenes sortase-anchored adhesin specifically expressed during infection in vivo.

Experimental Research into Metallic and Metal Oxide Nanoparticle Toxicity In Vivo

2017 ◽  
pp. 259-319 ◽  
Author(s):  
Boris A. Katsnelson ◽  
Larisa I. Privalova ◽  
Marina P. Sutunkova ◽  
Ilzira A. Minigalieva ◽  
Vladimir B. Gurvich ◽  
...  
Keyword(s):  
Metal Oxide ◽  
Experimental Research ◽  
Nanoparticle Toxicity ◽  
Metal Oxide Nanoparticle ◽  
Oxide Nanoparticle

Apoptotic Death of Listeria Monocytogenes-Infected Human Macrophages Induced by Lactoferricin B, A Bovine Lactoferrin-Derived Peptide

2005 ◽  
Vol 18 (2) ◽  
pp. 317-325 ◽  
Author(s):  
C. Longhi ◽  
M. P. Conte ◽  
S. Ranaldi ◽  
M. Penta ◽  
P. Valenti ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Bovine Milk ◽  
Cell Types ◽  
Bovine Lactoferrin ◽  
Human Macrophages ◽  
Apoptotic Death ◽  
Food Borne ◽  
Ifn Γ

Listeria monocytogenes, an intracellular facultative food-borne pathogen, was reported to induce apoptosis in vitro and in vivo in a variety of cell types with the exception of murine macrophages. These cells represent the predominant compartment of bacterial multiplication and die as a result of necrosis. In this study we showed that human non-activated and IFN-γ-activated macrophagic-like (THP-1) cells infected with L. monocytogenes, mainly die by necrosis rather than by an apoptotic process. Two natural products derived from bovine milk, lactoferrin and its derivative peptide lactoferricin B, are capable of regulating the fate of infected human macrophages. Bovine lactoferrin treatment of macrophages protects them from L. monocytogenes-induced death whereas lactoferricin B, its derivative peptide, determines a shifting of the equilibrium from necrosis to apoptosis.

Rapid hemostasis by nanofibers of polyhydroxyethyl methacrylate/polyglycerol sebacic acid: An in vitro / in vivo study

2020 ◽  
Vol 138 (5) ◽  
pp. 49785
Author(s):  
Jaleh Varshosaz ◽  
Zahra Choopannejad ◽  
Mohsen Minaiyan ◽  
Anousheh Zargar Kharazi
Keyword(s):  
Sebacic Acid ◽  
In Vivo Study ◽  
Polyhydroxyethyl Methacrylate

scholarly journals Advanced human in vitro models to assess metal oxide nanoparticle-cell interactions

MRS Bulletin ◽  
2014 ◽  
Vol 39 (11) ◽  
pp. 984-989 ◽  
Author(s):  
Peter Wick ◽  
Stefanie Grafmueller ◽  
Alke Petri-Fink ◽  
Barbara Rothen-Rutishauser
Keyword(s):  
Metal Oxide ◽  
In Vitro Models ◽  
Cell Interactions ◽  
Metal Oxide Nanoparticle ◽  
Oxide Nanoparticle ◽  
Image Position

Abstract

Formulation and Evaluation of Atorvastatin Calcium-Poly-ε-Caprolactone Nanoparticles Loaded Ocular Inserts for Sustained Release and Antiinflammatory Efficacy

2020 ◽  
Vol 21 (15) ◽  
pp. 1688-1698
Author(s):  
Germeen N.S. Girgis
Keyword(s):  
Sustained Release ◽  
In Vitro Release ◽  
Pluronic F127 ◽  
In Vivo Study ◽  
Average Weight ◽  
Drug Release Profile ◽  
Atorvastatin Calcium ◽  
Anti Inflammatory

Purpose: The work was performed to investigate the feasibility of preparing ocular inserts loaded with Poly-ε-Caprolactone (PCL) nanoparticles as a sustained ocular delivery system. Methods: First, Atorvastatin Calcium-Poly-ε-Caprolactone (ATC-PCL) nanoparticles were prepared and characterized. Then, the optimized nanoparticles were loaded within inserts formulated with Methylcellulose (MC) and Polyvinyl Alcohol (PVA) by a solvent casting technique and evaluated physically, for in-vitro drug release profile. Finally, an in-vivo study was performed on the selected formulation to prove non-irritability and sustained ocular anti-inflammatory efficacy compared with free drug-loaded ocuserts. Results: The results revealed (ATC-PCL) nanoparticles prepared with 0.5% pluronic F127 were optimized with 181.72±3.6 nm particle size, 0.12±0.02 (PDI) analysis, -27.4± 0.69 mV zeta potential and 62.41%±4.7% entrapment efficiency. Nanoparticles loaded ocuserts manifested compatibility between drug and formulation polymers. Moreover, formulations complied with average weight 0.055±0.002 to 0.143±0.023 mg, and accepted pH. ATC-PCL nanoparticles loaded inserts prepared by 5% MC showed more sustained, prolonged in-vitro release over 24h. In-vivo study emphasized non-irritability, ocular anti-inflammatory effectiveness represented by smaller lid closure scores, and statistically significant lowering in PMN count after 3h. Conclusion: These findings proposed a possibly simple, new and affordable price technique to prepare promising (ATC-PCL) nanoparticles loaded inserts to achieve sustained release with prolonged antiinflammatory efficacy.

Quercetin improve ischemia/reperfusion‐induced cardiomyocyte apoptosis in vitro and in vivo study via SIRT1/PGC‐1α signaling

2019 ◽  
Vol 120 (6) ◽  
pp. 9747-9757 ◽  
Author(s):  
Jiayou Tang ◽  
Linhe Lu ◽  
Yang Liu ◽  
Jipeng Ma ◽  
Lifang Yang ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Cardiomyocyte Apoptosis ◽  
In Vivo Study
Sign in / Sign up

Export Citation Format

Share Document