scholarly journals Study on the dependence of fluorescence intensity on optical density of solutions: the use of fluorescence observation field for inner filter effect corrections

2019 ◽  
Vol 18 (2) ◽  
pp. 583-591 ◽  
Author(s):  
Suraj Kumar Panigrahi ◽  
Ashok Kumar Mishra
Keyword(s):  
Optical Density ◽  
Fluorescence Intensity ◽  
Inner Filter Effect ◽  
Filter Effect ◽  
Fluorescence Observation ◽  
Density Of Solutions

Absorbance value that corresponds to maximum fluorescence intensity (Aflmax) depends on fluorescence observation field.

Inner Filter Effect on Fluorescence Dyes Spectra in Methanol Solution

2016 ◽  
Vol 675-676 ◽  
pp. 704-707 ◽  
Author(s):  
Saksit Sukprasong ◽  
Yongyut Manjit ◽  
Apichart Limpichaipanit ◽  
Athipong Ngamjarurojana
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Inner Filter Effect ◽  
Fluorescent Dye ◽  
Concentration Effect ◽  
Wavelength Shift ◽  
Filter Effect ◽  
High Concentrations ◽  
Fluorescence Dyes ◽  
Dye Solutions

This research was conducted to study the inner filter effect on the fluorescence spectra of fluorescence dyes. The concentration effect on all of fluorescent dye solutions showed the same trend in terms of changes in fluorescence intensity spectra. At low concentrations, the fluorescence intensity increased when the concentration of fluorescent dye solution increased. However, at high concentrations, the fluorescence intensity decreased when the concentration of fluorescent dye solution increased. Interestingly, the result of fluorescence spectra in dye solutions showed that the fluorescence intensity maxima in all dye solutions were shifted to a higher wavelength (red-shift) when the concentration of fluorescent dye solution increased. The results of concentration effect on fluorescence intensity and wavelength-shift in dyes solution can be explained by inner filter effect on fluorescent dye solutions.

scholarly journals “Turn on” Fluorescence Sensor of Glutathione Based on Inner Filter Effect of Co-Doped Carbon Dot/Gold Nanoparticle Composites

2021 ◽  
Vol 23 (1) ◽  
pp. 190
Author(s):  
Thi-Hoa Le ◽  
Ji-Hyeon Kim ◽  
Sang-Joon Park
Keyword(s):  
Gold Nanoparticle ◽  
Fluorescence Intensity ◽  
Limit Of Detection ◽  
Inner Filter Effect ◽  
Fluorescence Sensor ◽  
Fluorescence Emission Spectrum ◽  
Nitrogen And Phosphorus ◽  
Carbon Dot ◽  
Filter Effect ◽  
Co Doped

Glutathione (GSH) is a thiol that plays a significant role in nutrient metabolism, antioxidant defense and the regulation of cellular events. GSH deficiency is related to variety of diseases, so it is useful to develop novel approaches for GSH evaluation and detection. In this study we used nitrogen and phosphorus co-doped carbon dot-gold nanoparticle (NPCD–AuNP) composites to fabricate a simple and selective fluorescence sensor for GSH detection. We employed the reductant potential of the nitrogen and phosphorus co-doped carbon dots (NPCDs) themselves to form AuNPs, and subsequently NPCD–AuNP composites from Au3+. The composites were characterized by using a range of spectroscopic and electron microscopic techniques, including electrophoretic light scattering and X-ray diffraction. The overlap of the fluorescence emission spectrum of NPCDs and the absorption spectrum of AuNPs resulted in an effective inner filter effect (IFE) in the composite material, leading to a quenching of the fluorescence intensity. In the presence of GSH, the fluorescence intensity of the composite was recovered, which increased proportionally to increasing the GSH concentration. In addition, our GSH sensing method showed good selectivity and sensing potential in human serum with a limit of detection of 0.1 µM and acceptable results.

Polyethyleneimine-functionalized carbon dots as a fluorescent probe for doxorubicin hydrochloride by an inner filter effect

2021 ◽  
Vol 112 ◽  
pp. 110743
Author(s):  
Min Yang ◽  
Yujia Yan ◽  
Enzhou Liu ◽  
Xiaoyun Hu ◽  
Hong Hao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Inner Filter Effect ◽  
Doxorubicin Hydrochloride ◽  
Filter Effect

Fluorometric discrimination of tyrosine isomers based on the inner filter effect of chiral Au nanoparticles on MoS2 quantum dots

2021 ◽  
Author(s):  
Qianqian Zhao ◽  
Datong Wu ◽  
Zhengzhi Yin ◽  
Wenrong Cai ◽  
Haifeng Zhou ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Au Nanoparticles ◽  
Inner Filter Effect ◽  
Filter Effect

A fluorescent chiral sensor is proposed based on the inner filter effect (IFE) of chiral Au nanoparticles (AuNPs) on MoS2 quantum dots (MoS2 QDs), which can be used for the...

scholarly journals An ultrasensitive colorimetric and fluorescence dual-readout assay for glutathione with a carbon dot–MnO2 nanosheet platform based on the inner filter effect

RSC Advances ◽  
2021 ◽  
Vol 11 (34) ◽  
pp. 21137-21144
Author(s):  
Mengyuan He ◽  
Ning Shang ◽  
Bo Zheng ◽  
Gege Yue
Keyword(s):  
Human Body ◽  
Body Fluid ◽  
Inner Filter Effect ◽  
Carbon Dot ◽  
Filter Effect ◽  
Human Body Fluid ◽  
Mno2 Nanosheet

An ultrasensitive colorimetric and fluorescence dual-readout assay based on carbon dot–MnO2 nanosheets platform was developed for GSH detection in human body fluid samples.

A silicon nanoparticle based turn off fluorescent sensor for sudan I

2016 ◽  
Vol 8 (28) ◽  
pp. 5701-5706 ◽  
Author(s):  
Ammu Rosin Jose ◽  
Unni Sivasankaran ◽  
Shalini Menon ◽  
Krishnapillai Girish Kumar
Keyword(s):  
Fluorescent Sensor ◽  
Inner Filter Effect ◽  
Silicon Nanoparticle ◽  
Filter Effect ◽  
Sudan I

A turn off sensor for sudan I based on fluorescent Si NPs which works via an inner filter effect (IFE).

scholarly journals Investigating the Size-Dependent Binding of Pristine nC60 to Bovine Serum Albumin by Multi-Spectroscopic Techniques

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 298
Author(s):  
Shufang Liu ◽  
Shu’e Wang ◽  
Zhanzuo Liu
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Binding Constants ◽  
Inner Filter Effect ◽  
Spectroscopic Techniques ◽  
Size Distributions ◽  
Size Dependent ◽  
Filter Effect ◽  
Vis Spectroscopy

The morphology of nanomaterials may affect their interaction with biomacromolecules such as proteins. Previous work has studied the size-dependent binding of pristine nC60 to bovine/human serum albumin using the fluorometric method and found that the fluorescence inner filter effect might affect this interaction. However, if it is necessary to accurately calculate and obtain binding information, the fluorescence inner filter effect should not be ignored. This work aimed to further investigate the effect of the fluorescence inner filter on the interaction between pristine nC60 with different particle sizes (140–160, 120–140, 90–110, 50–70, and 30–50 nm) and bovine serum albumin for a more accurate comprehension of the binding of pristine nC60 to bovine serum albumin. The nC60 nanoparticles with different size distributions used in the experiments were obtained by the solvent displacement and centrifugation method. UV-Vis spectroscopy and fluorescence spectroscopy were used to study the binding of nC60 with different size distributions to bovine serum albumin (BSA) before and after eliminating the fluorescence inner filter effect. The results showed that the fluorescence inner filter effect had an influence on the interaction between nC60 and proteins to some extent, and still did not change the rule of the size-dependent binding of nC60 nanoparticles to BSA. Further studies on the binding parameters (binding constants and the number of binding sites) between them were performed, and the effect of the binding on BSA structures and conformation were also speculated.

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