scholarly journals Nanomaterials and continuous wave laser-based efficient desorption for atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices

RSC Advances ◽  
2018 ◽  
Vol 8 (15) ◽  
pp. 8021-8025 ◽  
Author(s):  
Jae Young Kim ◽  
Eun Seok Seo ◽  
Hee Jin Lim ◽  
Hyunmin Kim ◽  
Ji-Won Park ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Continuous Wave ◽  
Mass Spectrometric ◽  
Continuous Wave Laser ◽  
Mass Spectrometric Imaging ◽  
Tissue Slices ◽  
Hippocampal Tissue ◽  
Wave Laser ◽  
532 Nm ◽  
Resolution Mass

Micrometer-resolution mass spectrometric imaging of live hippocampal tissue is achieved with a highly efficient desorption of biomolecules using a 532 nm continuous wave laser and gold nanoparticles or graphene oxide as an energy transporter.

A high-power and low-noise 532-nm continuous-wave laser for quantum gas microscopy

2021 ◽  
Vol 92 (8) ◽  
pp. 083202
Author(s):  
Meng-Da Li ◽  
Yong-Guang Zheng ◽  
Wei-Yong Zhang ◽  
Xuan-Kai Wang ◽  
Bo Xiao ◽  
...  
Keyword(s):  
High Power ◽  
Continuous Wave ◽  
Low Noise ◽  
Continuous Wave Laser ◽  
Quantum Gas ◽  
Wave Laser ◽  
532 Nm

Gold-Nanoparticle Layer Substrate Assisted Transmission-Mode Laser Desorption for Atmospheric Pressure Mass Spectrometry Imaging

2020 ◽  
Vol 12 (10) ◽  
pp. 1517-1523
Author(s):  
Jae Young Kim ◽  
Heejin Lim ◽  
Sun Young Lee ◽  
Gwanjin Lee ◽  
Dong-Kwon Lim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gold Nanoparticle ◽  
Atmospheric Pressure ◽  
Mass Spectrometry Imaging ◽  
Continuous Wave ◽  
Continuous Wave Laser ◽  
Fresh Tissue ◽  
Mode Laser ◽  
Nanoparticle Layer ◽  
Wave Laser

We demonstrate continuous-wave laser-based ambient mass spectrometry imaging with the use of a gold-nanoparticle layer substrate. When a fresh tissue slice is placed on a gold-nanoparticle layer substrate and irradiated with a 532-nm continuous-wave laser, the transmission-mode laser configuration provides precise desorption performance to facilitate mass spectrometry imaging. The subsequent ionization process with nonthermal atmospheric pressure plasma jets generates sufficient amounts of molecular ions. By using this method, micrometer-spatial-resolution mass spectrometry imaging of humid tissues can be obtained. The gold-nanoparticle layer substrates can be prepared and stored in advance of the experiment, resulting in simplified specimen preparation and an advantage in faster preparing of fresh tissue specimen for analysis.

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