Pilot-scale production of a highly thermostable α-amylase enzyme from Thermotoga petrophila cloned into E. coli and its application as a desizer in textile industry

In studies of the late medieval economy, the dominant position of the textile industry has long been recognized. Since the opening decades of this century however, scholarly attention has been directed almost exclusively toward the luxury industries of silk and fine woolens, which involved a complex financial and commercial structure geared to the satisfaction of the needs and desires of a wealthy and select clientele. Relatively neglected is that branch of the textile industry devoted to the production of low-priced cotton cloth for popular consumption. This neglect is all the more surprising in view of a rich if somewhat dispersed documentation attesting to the importance of this industry in numerous towns of Northern Italy. The large-scale production of cotton cloth posed problems of financing and organization not unlike those of silk and wool and gave rise to similar entrepreneurial forms. At the same time a study of the organization of cotton manufacture provides a number of unique insights into aspects of economic organization in Northern Italy. In the period covered by this paper, the cotton manufacturing centers of this area formed a single production zone characterized by a high degree of economic interdependence and a marked tendency toward the standardization of products.

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MICROCLEAR: GREEN TECHNOLOGY FOR TREATING AND RECYCLING OF COLOURED WASTEWATER

Jurnal Teknologi ◽

10.11113/jt.v77.6918 ◽

2015 ◽

Vol 77 (31) ◽

Author(s):

Zaharah Ibrahim ◽

Adibah Yahya ◽

Azmi Aris ◽

Ifnu Hakim ◽

Mohd Ariff Taib ◽

...

Keyword(s):

Textile Industry ◽

Large Scale ◽

Treatment Plant ◽

Textile Wastewater ◽

Good Alternative ◽

Pilot Scale ◽

Green Technology ◽

Scale Production ◽

Continuous Growth ◽

Set Up

The continuous growth and demand for our textiles and textile products have resulted in the generation of highly polluted and coloured wastewater emanating from the textile industries. These are detrimental to the environment and pose health threats to the human population if not properly treated. The treatment of colour is a great challenge over the last decades and until now, there is no single and economical treatment process. As effective treatment plant is generally expensive and unaffordable; a good alternative and timely solution is the utilisation of specialised group of microbes called Microclear. These microorganisms have the abilities to decolourise and transform coloured compounds into simpler and non-hazardous compounds without the use of chemicals. Intensive fundamental studies and also the application of the Microclear at the bench and pilot scale (sequential 1000 L and 2000 L) reactors to treat real wastewater were carried out. The microbes can also be applied directly into the existing treatment plant or ponding systems without the use of a commercial reactor. . Under the UTM-MTDC symbiosis program, Microclear Sdn. Bhd. was set up and work is in progress for large scale production of microbes to treat real textile wastewater in a demo plant of 150,000 L capacity located at the textile industry.

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Large-scale Production of Novel Porcine Circovirus Type 2d (PCV2d) Subunit Vaccine Using E. coli Platform

10.22541/au.163255824.44486305/v1 ◽

2021 ◽

Author(s):

Sarawuth Noppiboon ◽

Thunyaluk Kerdboon ◽

Neeracha Lapanusorn ◽

Pisit Ekkpongpaisit ◽

Sarah Slack ◽

...

Keyword(s):

Large Scale ◽

Pilot Scale ◽

Porcine Circovirus Type ◽

Exchange Chromatography ◽

Porcine Circovirus ◽

Scale Production ◽

E Coli ◽

Large Scale Production ◽

Production Platform ◽

Cost Efficient

Porcine Circovirus type 2d (PCV2d) is becoming the predominant PCV genotype and considerably affects the global pig industry. Nevertheless, currently no commercial PCV2d vaccine is available. Preventing and controlling the disease caused by PCV2d is therefore based on other genotype-based vaccines. However, their production platforms are laborious, limited in expression level and relatively expensive for veterinary applications. To address these challenges, we have developed a simple and cost-efficient platform for a novel PCV2d vaccine production platform, using fed-batch E. coli fermentation followed by cell disruption and filtration, and a single purification step via cation exchange chromatography. The process was developed at bench scale and then pilot scale where the PCV2d subunit protein yield was approximately 0.8 g/L fermentation volume in a short production time. Moreover, we have successfully implemented this production process at two different sites, in Southeast Asia and Europe. This demonstrates transferability and the high potential for successful industrial production.

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Pilot-Scale Production of Chito-Oligosaccharides Using an Innovative Recombinant Chitosanase Preparation Approach

Polymers ◽

10.3390/polym13020290 ◽

2021 ◽

Vol 13 (2) ◽

pp. 290

Author(s):

Chih-Yu Cheng ◽

Chia-Huang Tsai ◽

Pei-Jyun Liou ◽

Chi-Hang Wang

Keyword(s):

Cell Density ◽

Scale Up ◽

Cost Effective ◽

Pilot Scale ◽

Ionic Concentration ◽

Dihydrogen Phosphate ◽

Scale Production ◽

Sodium Dihydrogen Phosphate ◽

Selective Precipitation ◽

Pilot Scale Production

For pilot-scale production of chito-oligosaccharides, it must be cost-effective to prepare designable recombinant chitosanase. Herein, an efficient method for preparing recombinant Bacillus chitosanase from Escherichia coli by elimination of undesirable substances as a precipitate is proposed. After an optimized culture with IPTG (Isopropyl β-d-1-thiogalactopyranoside) induction, the harvested cells were resuspended, disrupted by sonication, divided by selective precipitation, and stored using the same solution conditions. Several factors involved in these procedures, including ion types, ionic concentration, pH, and bacterial cell density, were examined. The optimal conditions were inferred to be pH = 4.5, 300 mM sodium dihydrogen phosphate, and cell density below 1011 cells/mL. Finally, recombinant chitosanase was purified to >70% homogeneity with an activity recovery and enzyme yield of 90% and 106 mg/L, respectively. When 10 L of 5% chitosan was hydrolyzed with 2500 units of chitosanase at ambient temperature for 72 h, hydrolyzed products having molar masses of 833 ± 222 g/mol with multiple degrees of polymerization (chito-dimer to tetramer) were obtained. This work provided an economical and eco-friendly preparation of recombinant chitosanase to scale up the hydrolysis of chitosan towards tailored oligosaccharides in the near future.

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Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652010000400017 ◽

2010 ◽

Vol 82 (4) ◽

pp. 941-951 ◽

Cited By ~ 2

Author(s):

Cui Yu-bao ◽

Ying Zhou ◽

Shi Weihong ◽

Ma Guifang ◽

Li Yang ◽

...

Keyword(s):

Large Scale ◽

Alpha Helix ◽

Random Coil ◽

Reference Sequence ◽

Scale Production ◽

Dermatophagoides Farinae ◽

E Coli ◽

Large Scale Production ◽

Solid Foundation ◽

Group 2

To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2), the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580). The cDNA was bound to vector pET28a to construct plasmid pET28a(+)-Der f 2, which was transformed into E. coli BL21 and induced by IPTG. SDS-PAGE showed a specific band of about 14kDa in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum IgE from asthmatic children. The protein had a signal peptide of 17 amino acids. Its secondary structure comprised an alpha helix (19.86%), an extended strand (30.82%), and a random coil (49.32%). The subcellular localization of this allergen was predicted to be at mitochondria. Furthermore, its function was shown to be associated with an MD-2-related lipid-recognition (ML) domain. The results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.

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