scholarly journals Various antibacterial mechanisms of biosynthesized copper oxide nanoparticles against soilborneRalstonia solanacearum

RSC Advances ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 3788-3799 ◽  
Author(s):  
Juanni Chen ◽  
Shuyu Mao ◽  
Zhifeng Xu ◽  
Wei Ding
Keyword(s):  
Gene Expression ◽  
Copper Oxide ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biofilm Formation ◽  
Cell Metabolism ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles

Green synthesized CuONPs disturb cell metabolism, biofilm formation, physical motility and gene expression inRalstonia solanacearum, thereby effectively controlling bacterial wilt.

Effect of copper oxide nanoparticles on gene expression of NMDA receptor

2021 ◽  
Vol 29 (6) ◽  
pp. 60-66
Author(s):  
Ivan Andreevich Sitnikov ◽  
Daria Ramilevna Shaikhova ◽  
Anna Mikhailovna Amromina ◽  
Marina Petrovna Sutunkova ◽  
Yuliya Vladimirovna Ryabova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nmda Receptor ◽  
Copper Oxide ◽  
Clinical Manifestations ◽  
Copper Oxide Nanoparticles ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Genes Encoding ◽  
Effect Of Copper

Introduction. Copper plays an important role in the metabolism of the brain, but particles of copper, in the nanometer range, exhibit neurotoxic properties and cause malfunctioning of brain cells. Material and methods. For 6 weeks, 3 times a week, the animals were injected with a suspension of NPs of copper oxide. The determination of the expression of the genes GRIN1, GRIN2a, and GRIN2b, encoding the proteins GluN1, GluN2a, and GluN2b, respectively, was carried out by real-time PCR with probes. Results. A statistically significant decrease in the expression level of genes encoding NMDA receptor proteins was determined when exposed to 0.5 mg/ml CuO nanoparticles (ΔCt(GRIN1) = 0.813; ΔCt(GRIN2A) = 3.477; ΔCt(GRIN2B) = 1.37) in comparison with control group (ΔCt(GRIN1) = 6.301; ΔCt(GRIN2A) = 7.823; ΔCt(GRIN2B) = 4.747). Conclusion. Evaluation of gene expression of the NMDA receptor may be present in a genetic marker to determine the toxic effect of copper oxide nanoparticles; however, further studies are needed, including behavioral tests to confirm the clinical manifestations of neurodegenerative disorders.

scholarly journals Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Elham Ansarifard ◽  
Zahra Zareshahrabadi ◽  
Najmeh Sarafraz ◽  
Kamiar Zomorodian
Keyword(s):  
Copper Oxide ◽  
Biofilm Formation ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Significant Activity ◽  
Cuo Nps ◽  
Denture Liners ◽  
Oral Pathogens ◽  
Soft Denture Liner ◽  
Scanning Electron

Objectives. Soft denture liners provide a favorable environment for adhesion and colonization of microorganisms. This in vitro study aimed to examine the efficacy of different concentrations of copper oxide nanoparticles (CuO NPs) incorporation into soft denture liner on the biofilm formation of the microbial species. Methods. Field Emission Scanning Electron Microscopy (FESEM) images from NPs were recorded. Antifungal susceptibility testing of CuO NPs against five standard strains of Candida albicans (CBS 10261, 1905, 1912, 1949, 2730), Streptococcus mutans (ATCC35668), Streptococcus sobrinus (ATCC27607), and Streptococcus salivarius (ATCC9222) was performed by the broth microdilution method with the Clinical and Laboratory Standards Institute reference method. The biofilm inhibition percentages of CuO NPs on the soft denture liners were determined by XTT assay. Results. The characterization of CuO NPs by scanning electron microscope (SEM) analyses confirmed the synthesis of NPs with appropriate structure and size with a mean diameter of 18.3 ± 9.1 nm. The CuO NPs successfully inhibited the growth of the tested standard strains of C. albicans and Streptococcus spp. at concentrations ranging from 64 to 128 µg mL−1. Indeed, incorporation of CuO NPs at a concentration of 500 µg mL−1 into the soft denture liners exhibited a significant activity (75%) in inhibition of C. albicans. biofilm formation in a dose-dependent manner. The biofilm formation of C. albicans in the presence of CuO NPs was lower than Streptococcus spp. in comparison with the control group ( p < 0.05 ). Conclusion. Incorporation of CuO NPs significantly decreased the colonization and plaque formation of the oral pathogens, especially C. albicans accumulation. These NPs may be useful as a promising agent for the antimicrobial management of soft denture liner materials.

scholarly journals Structural Analysis of Chemical and Green Synthesis of CuO Nanoparticles and their Effect on Biofilm Formation

2018 ◽  
Vol 15 (2) ◽  
pp. 211-216 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Copper Oxide ◽  
Biofilm Formation ◽  
Chemical Method ◽  
Copper Oxide Nanoparticles ◽  
Cuo Nanoparticles ◽  
Crystalline Size ◽  
Oxide Nanoparticles ◽  
Average Crystalline Size ◽  
Green Method ◽  
Cuo Nps

Copper oxide nanoparticles (CuO NPs) were synthesized by two methods. The first was chemical method by using copper nitrate Cu (NO3)2 and NaOH, while the second was green method by using Eucalyptus camaldulensis leaves extract and Cu (NO3)2. These methods easily give a large scale production of CuO nanoparticles. X-ray diffraction pattern (XRD) reveals single phase monoclinic structure. The average crystalline size of CuO NPs was measured and used by Scherrer equation which found 44.06nm from chemical method, while the average crystalline size was found from green method was 27.2nm. The morphology analysis using atomic force microscopy showed that the grain size for CuO NPs was synthesized by chemical and green methods were 77.70 and 89.24 nm, respectively. The effectiveness of copper oxide nanoparticles on bacteria was measured for both gram positive, negative and fungi, copper oxide minutes showed excellent efficacy on biofilm formation.

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

Green synthesis of copper oxide nanoparticles using aloe vera extract

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Hemalatha D ◽  
Saraswath S
Keyword(s):  
Copper Oxide ◽  
Biomedical Application ◽  
Aloe Vera ◽  
Copper Oxide Nanoparticles ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Cupric Sulfate ◽  
X Ray Diffraction ◽  
Fouriertransform Infrared Spectroscopy ◽  
Effective Use

In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.

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