Transcriptional profiling reveals gene expression changes associated with inflammation and cell proliferation following short-term inhalation exposure to copper oxide nanoparticles

2017 ◽  
Vol 38 (3) ◽  
pp. 385-397 ◽  
Author(s):  
Pedro M. Costa ◽  
Ilse Gosens ◽  
Andrew Williams ◽  
Lucian Farcal ◽  
Daniele Pantano ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Copper Oxide ◽  
Transcriptional Profiling ◽  
Inhalation Exposure ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Short Term

Effect of copper oxide nanoparticles on gene expression of NMDA receptor

2021 ◽  
Vol 29 (6) ◽  
pp. 60-66
Author(s):  
Ivan Andreevich Sitnikov ◽  
Daria Ramilevna Shaikhova ◽  
Anna Mikhailovna Amromina ◽  
Marina Petrovna Sutunkova ◽  
Yuliya Vladimirovna Ryabova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nmda Receptor ◽  
Copper Oxide ◽  
Clinical Manifestations ◽  
Copper Oxide Nanoparticles ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Genes Encoding ◽  
Effect Of Copper

Introduction. Copper plays an important role in the metabolism of the brain, but particles of copper, in the nanometer range, exhibit neurotoxic properties and cause malfunctioning of brain cells. Material and methods. For 6 weeks, 3 times a week, the animals were injected with a suspension of NPs of copper oxide. The determination of the expression of the genes GRIN1, GRIN2a, and GRIN2b, encoding the proteins GluN1, GluN2a, and GluN2b, respectively, was carried out by real-time PCR with probes. Results. A statistically significant decrease in the expression level of genes encoding NMDA receptor proteins was determined when exposed to 0.5 mg/ml CuO nanoparticles (ΔCt(GRIN1) = 0.813; ΔCt(GRIN2A) = 3.477; ΔCt(GRIN2B) = 1.37) in comparison with control group (ΔCt(GRIN1) = 6.301; ΔCt(GRIN2A) = 7.823; ΔCt(GRIN2B) = 4.747). Conclusion. Evaluation of gene expression of the NMDA receptor may be present in a genetic marker to determine the toxic effect of copper oxide nanoparticles; however, further studies are needed, including behavioral tests to confirm the clinical manifestations of neurodegenerative disorders.

Short-term exposure to low concentrations of copper oxide nanoparticles can negatively impact the ecological performance of a cosmopolitan freshwater fungus

2019 ◽  
Vol 21 (12) ◽  
pp. 2001-2007 ◽  
Author(s):  
Sahadevan Seena ◽  
Santosh Kumar
Keyword(s):  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Short Term ◽  
Ecological Processes ◽  
Cuo Nps ◽  
Ecological Performance ◽  
Low Concentrations ◽  
Short Term Exposure ◽  
Freshwater Fungus

Short term exposure to very low concentrations of CuO NPs can have an impact on freshwater ecological processes.

scholarly journals Various antibacterial mechanisms of biosynthesized copper oxide nanoparticles against soilborneRalstonia solanacearum

RSC Advances ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 3788-3799 ◽  
Author(s):  
Juanni Chen ◽  
Shuyu Mao ◽  
Zhifeng Xu ◽  
Wei Ding
Keyword(s):  
Gene Expression ◽  
Copper Oxide ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biofilm Formation ◽  
Cell Metabolism ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles

Green synthesized CuONPs disturb cell metabolism, biofilm formation, physical motility and gene expression inRalstonia solanacearum, thereby effectively controlling bacterial wilt.

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

Green synthesis of copper oxide nanoparticles using aloe vera extract

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Hemalatha D ◽  
Saraswath S
Keyword(s):  
Copper Oxide ◽  
Biomedical Application ◽  
Aloe Vera ◽  
Copper Oxide Nanoparticles ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Cupric Sulfate ◽  
X Ray Diffraction ◽  
Fouriertransform Infrared Spectroscopy ◽  
Effective Use

In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.

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