The effects of aloe emodin-mediated antimicrobial photodynamic therapy on drug-sensitive and resistant Candida albicans

Aloe emodin-mediated antimicrobial photodynamic therapy effectively inactivated drug-sensitive and resistant C. albicans and caused damage to the cell wall, cytoplasm, and nucleus.

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Aloe-emodin-mediated antimicrobial photodynamic therapy against multidrug-resistant Acinetobacter baumannii: an in vivo study

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2021.102311 ◽

2021 ◽

pp. 102311

Author(s):

Yang Wang ◽

Jiao Li ◽

Songmei Geng ◽

Xiaopeng Wang ◽

Zixin Cui ◽

...

Keyword(s):

Photodynamic Therapy ◽

Acinetobacter Baumannii ◽

Multidrug Resistant ◽

In Vivo Study ◽

Antimicrobial Photodynamic Therapy ◽

Aloe Emodin

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Copolymer-nanocapsules of zinc phenyl-thio-phthalocyanine and amphotericin-B in association with antimicrobial photodynamic therapy (A-PDT) applications against Candida albicans yeasts

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2021.102273 ◽

2021 ◽

Vol 34 ◽

pp. 102273

Author(s):

Rodrigo P. Evangelista ◽

Camila F. Amantino ◽

Rosemeire C.L.R. Pietro ◽

Rodrigo Sorrechia ◽

Rodolfo D. Piazza ◽

...

Keyword(s):

Candida Albicans ◽

Photodynamic Therapy ◽

Amphotericin B ◽

Antimicrobial Photodynamic Therapy

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DNase increases the efficacy of antimicrobial photodynamic therapy on Candida albicans biofilms

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2019.05.038 ◽

2019 ◽

Vol 27 ◽

pp. 124-131 ◽

Cited By ~ 4

Author(s):

Beatriz H.D. Panariello ◽

Marlise I. Klein ◽

Fernanda Alves ◽

Ana Cláudia Pavarina

Keyword(s):

Candida Albicans ◽

Photodynamic Therapy ◽

Antimicrobial Photodynamic Therapy ◽

Candida Albicans Biofilms

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Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans

Frontiers in Medicine ◽

10.3389/fmed.2021.641244 ◽

2021 ◽

Vol 8 ◽

Author(s):

Thomas Voit ◽

Fabian Cieplik ◽

Johannes Regensburger ◽

Karl-Anton Hiller ◽

Anita Gollmer ◽

...

Keyword(s):

Cell Wall ◽

Candida Albicans ◽

Fungal Infections ◽

Cell Envelope ◽

Antimicrobial Photodynamic Therapy ◽

Fungal Cell ◽

Before And After ◽

Antifungal Action ◽

Complete Cell ◽

Further Development

The antimicrobial photodynamic therapy (aPDT) is a promising approach for the control of microbial and especially fungal infections such as mucosal mycosis. TMPyP [5,10,15, 20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate] is an effective photosensitizer (PS) that is commonly used in aPDT. The aim of this study was to examine the localization of TMPyP in Candida albicans before and after irradiation with visible light to get information about the cellular mechanism of antifungal action of the photodynamic process using this PS. Immediately after incubation of C. albicans with TMPyP, fluorescence microscopy revealed an accumulation of the PS in the cell envelope. After irradiation with blue light the complete cell showed red fluorescence, which indicates, that aPDT is leading to a damage in the cell wall with following influx of PS into the cytosol. Incubation of C. albicans with Wheat Germ Agglutinin (WGA) could confirm the cell wall as primary binding site of TMPyP. The finding that the porphyrin accumulates in the fungal cell wall and does not enter the interior of the cell before irradiation makes it unlikely that resistances can emerge upon aPDT. The results of this study may help in further development and modification of PS in order to increase efficacy against fungal infections such as those caused by C. albicans.

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Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by Candida albicans Resistant to Fluconazole

Pharmaceuticals ◽

10.3390/ph12030140 ◽

2019 ◽

Vol 12 (3) ◽

pp. 140 ◽

Cited By ~ 1

Author(s):

Karem Janeth Rimachi Hidalgo ◽

Juliana Cabrini Carmello ◽

Cláudia Carolina Jordão ◽

Paula Aboud Barbugli ◽

Carlos Alberto de Sousa Costa ◽

...

Keyword(s):

Candida Albicans ◽

Photodynamic Therapy ◽

Combined Therapy ◽

Oral Candidiasis ◽

Confocal Scanning Laser Microscopy ◽

Antimicrobial Photodynamic Therapy ◽

Promising Alternative ◽

Confocal Scanning ◽

Confocal Scanning Laser

Background: It has been demonstrated that azole-resistant strains of Candida albicans have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Methods: Mice were infected with fluconazole-resistant C. albicans (ATCC 96901). To perform the combined therapy, aPDT, mediated by Photodithazine (PDZ) and LED light, was used together with NYS. The efficacy of the treatments was evaluated by microbiological, macroscopic, histopathological and Confocal Scanning Laser Microscopy analyses of the lesions. The expression of p21 and p53, proteins associated with cell death, from the tongues of mice, was also performed. Results: The combined therapy reduced the fungal viability by around 2.6 log10 and decreased the oral lesions and the inflammatory reaction. Additionally, it stimulated the production of p53 and p21. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by C. albicans resistant to fluconazole.

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