scholarly journals Aloe‐emodin‐mediated antimicrobial photodynamic therapy against dermatophytosis caused by Trichophyton rubrum

2021 ◽  
Author(s):  
Wenpeng Ma ◽  
Miaomiao Zhang ◽  
Zixin Cui ◽  
Xiaopeng Wang ◽  
Xinwu Niu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Trichophyton Rubrum ◽  
Antimicrobial Photodynamic Therapy ◽  
Aloe Emodin

scholarly journals Antifungal Effect of Antimicrobial Photodynamic Therapy Mediated by Haematoporphyrin Monomethyl Ether and Aloe Emodin on Malassezia furfur

2021 ◽  
Vol 12 ◽  
Author(s):  
Zixin Cui ◽  
Miaomiao Zhang ◽  
Songmei Geng ◽  
Xinwu Niu ◽  
Xiaopeng Wang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Monomethyl Ether ◽  
Antimicrobial Efficacy ◽  
Irradiation Condition ◽  
Dependent Manner ◽  
Antimicrobial Photodynamic Therapy ◽  
Malassezia Furfur ◽  
Dermatological Diseases ◽  
Intracellular Ros ◽  
Aloe Emodin

Infectious dermatological diseases caused by Malassezia furfur are often chronic, recurrent, and recalcitrant. Current therapeutic options are usually tedious, repetitive, and associated with adverse effects. Alternatives that broaden the treatment options and reduce side effects for patients are needed. Antimicrobial photodynamic therapy (aPDT) is an emerging approach that is quite suitable for superficial infections. The aim of this study is to investigate the antimicrobial efficacy and effect of aPDT mediated by haematoporphyrin monomethyl ether (HMME) and aloe emodin (AE) on clinical isolates of M. furfur in vitro. The photodynamic antimicrobial efficacy of HMME and AE against M. furfur was assessed by colony forming unit (CFU) assay. The uptake of HMME and AE by M. furfur cells was investigated by fluorescence microscopy. Reactive oxygen species (ROS) probe and flow cytometry were employed to evaluate the intracellular ROS level. The effect of HMME and AE-mediated aPDT on secreted protease and lipase activity of M. furfur was also investigated. The results showed that HMME and AE in the presence of light effectively inactivated M. furfur cells in a photosensitizer (PS) concentration and light energy dose-dependent manner. AE exhibited higher antimicrobial efficacy against M. furfur than HMME under the same irradiation condition. HMME and AE-mediated aPDT disturbed the fungal cell envelop, significantly increased the intracellular ROS level, and effectively inhibited the activity of secreted protease and lipase of M. furfur cells. The results suggest that HMME and AE have potential to serve as PSs in the photodynamic treatment of dermatological diseases caused by M. furfur, but further ex vivo or in vivo experiments are needed to verify that they can meet the requirements for clinical practice.

scholarly journals The effects of aloe emodin-mediated antimicrobial photodynamic therapy on drug-sensitive and resistant Candida albicans

2020 ◽  
Vol 19 (4) ◽  
pp. 485-494 ◽  
Author(s):  
Wenpeng Ma ◽  
Chengcheng Liu ◽  
Jiao Li ◽  
Ming Hao ◽  
Yanhong Ji ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Aloe Emodin

Aloe emodin-mediated antimicrobial photodynamic therapy effectively inactivated drug-sensitive and resistant C. albicans and caused damage to the cell wall, cytoplasm, and nucleus.

Regenerative treatment of peri-implantitis following implant surface decontamination with titanium brush and antimicrobial photodynamic therapy: a case series with reentry

2020 ◽  
Author(s):  
Pier Poli ◽  
Francisley Avila Souza ◽  
Mattia Manfredini ◽  
Carlo Maiorana ◽  
Mario Beretta
Keyword(s):  
Photodynamic Therapy ◽  
Clinical Case ◽  
Case Series ◽  
Antimicrobial Photodynamic Therapy ◽  
Implant Surface ◽  
Surface Decontamination

Not required for Clinical case letters according to the authors' guidelines.

scholarly journals Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 869
Author(s):  
Beatriz Müller Nunes Souza ◽  
Juliana Guerra Pinto ◽  
André Henrique Correia Pereira ◽  
Alejandro Guillermo Miñán ◽  
Juliana Ferreira-Strixino
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Bacterial Inactivation ◽  
Bacterial Reduction ◽  
Complete Inactivation ◽  
Opportunistic Bacteria ◽  
Significant Difference ◽  
Mrsa Strains

Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.

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