Monospecies and multispecies biofilms of Candida albicans , Staphylococcus aureus , Staphylococcus epidermidis and Pseudomonas aeruginosa are affected by antimicrobial photodynamic therapy

2017 ◽  
Vol 17 ◽  
pp. A45
Author(s):  
C.A. Pereira-Correia ◽  
A.O.C. Jorge
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Photodynamic Therapy ◽  
Multispecies Biofilms

scholarly journals The effect of some essential oils on pathogenes that cause eczema

2020 ◽  
Author(s):  
Meryem Karacam ◽  
Durmus Alpaslan Kaya
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Myrtus Communis ◽  
Plant Essential Oils ◽  
Myrtus Communis L ◽  
Thymbra Spicata

In this study, the antimicrobial activity of essential oils obtained from Thymbra spicata L., Lavandula angustifolia Mill. and Myrtus communis L. on the pathogens causing eczema Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 12228), Escheria coli (ATCC 25922), Acinetobacter baumannii (ATCC 43498), Pseudomonas aeruginosa (ATCC 27853) ve Candida albicans (ATCC 90028) were investigated. The MIC and MBC values of the essential oils used in the study against the pathogens causing eczema were determined. As a result of the results obtained, antimicrobial activity of plant essential oils used in the study on test microorganisms was determined. Among the essential oils, it was found that the most effective essential oil was thyme followed by the lavender.

Chalcogenide nanoparticles and organic photosensitizers for synergetic antimicrobial photodynamic therapy

2021 ◽  
Author(s):  
Carlos Garin ◽  
Teresa Alejo ◽  
Vanesa Pérez Laguna ◽  
Martin Prieto ◽  
Gracia Mendoza ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Copper Sulfide ◽  
Pathogenic Bacteria ◽  
Antimicrobial Photodynamic Therapy ◽  
Wild Type ◽  
Antimicrobial Effects

Synergistic antimicrobial effects were observed for copper sulfide (CuS) nanoparticles together with indocyanine green (ICG) in the elimination of wild type pathogenic bacteria (Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa...

scholarly journals SEASONAL FEATURES OF THE MICROBIAL LANDSCAPE OF PALATINE TONSILS IN HEALTHY STUDENTS

2018 ◽  
pp. 98-103
Author(s):  
E. L. Savlevich ◽  
M. A. Ivanova ◽  
M. A. Mokronosova ◽  
S. A. Gorbunov ◽  
A. P. Yakushenkova
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Streptococcus Pyogenes ◽  
Staphylococcus Epidermidis ◽  
Acute Respiratory Infection ◽  
Clinical Symptoms ◽  
Healthy Individuals ◽  
Palatine Tonsils ◽  
Landscape Characteristics

Aim.To find out microbial landscape characteristics of the palatine tonsils in healthy students, depending on the season. Materials and methods. It were examined 40 students without any oropharyngeal pathology aged 17-30 (23 ± 3.5). There were no signs of chronic tonsillitis at pha-ryngoscopy, no signs of acute respiratory infection; they did not take antibiotics for one last month prior to the study and had no tonsilitis for the last 5 years. Examination was made three times, once in a season: autumn from October 16 till November 2; winter from January 27 till March 6 ; spring from April 2 till April 19. Results. The normal microlandscape in healthy individuals aged 17-30 years permanently had Streptococcus viridans, Streptococcus pyogenes, Staphylococcus aureus, Staphylococcus epidermidis, Candida albicans, Neisseria, Micrococcus, Bacillus, and Pseudomonas aeruginosa. Presence of S. pyogenes andP. aeruginosa in the structure of microlandscape of the palatine tonsils did not cause any clinical symptoms in their carriers. We also revealed a seasonal dynamics expressed by the frequency of the increase of conditionally pathogenic flora on palatine tonsils in spring and the decrease in all microorganisms growth in winter.

scholarly journals Actividad antimicrobiana de cuatro plantas del nor-oriente peruano

2009 ◽  
Vol 12 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Julio R. Ruiz Q. ◽  
Mirtha Roque A.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Staphylococcus Epidermidis ◽  
Microsporum Canis ◽  
Terminalia Catappa

Se investigó la actividad antimicrobiana in vitro de los extractos metanólicos, etanólicos e hidroalcohólicos de cuatro plantas del nor-oriente peruano: Cassia reticulata (planta entera), Ilex guayusa Loes (hojas), Piper lineatum (hojas), y Terminalia catappa (hojas). Las especies fueron recolectadas en el departamento de Cajamarca, excepto Terminalia catappa (Amazonas). La actividad antimicrobiana se evaluó mediante el método de difusión en agar. Los microorganismos utilizados fueron las bacterias Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacillus subtilis y Escherichia coli; y los hongos Candida albicans, Aspergillus niger y Microsporum canis. De doce extractos investigados, ocho (67%) presentaron actividad antimicrobiana significativa frente a Staphylococcus aureus y Staphylococcus epidermidis y uno (8%) frente a Escherichia coli. De doce extractos investigados, diez (83%) presentaron actividad significativa frente a Candida albicans, y seis (50%) contra Microsporum canis. Los extractos con la mejor actividad actimicrobiana fueron los tres extractos del Piper lineatum; el extracto hidroalcohólico de Cassia reticulata y el hidroalcohólico de Terminalia catappa.

scholarly journals Antimicrobial activity of propolis agents on some pathogenic microbes

2010 ◽  
Vol 7 (1) ◽  
pp. 269-276
Author(s):  
Baghdad Science Journal
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Synergistic Effect ◽  
Staphylococcus Epidermidis ◽  
Alcohol Concentration ◽  
Pathogenic Microorganisms ◽  
Pathogenic Microbes ◽  
Method Of Extraction

The study aims to investigate the antimicrobial activity of propolis obtained from different regions of Iraq compared with that of propolis obtained from Iran. Samples were investigated for their antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Eschericha coli, Klebsiella pneumoniae, Bacillus cereus , Staphylococcus epidermidis and Candida albicans using standard antimicrobial assays. Marked variations in the antimicrobial activity of the different propolis samples were observed, the method of extraction selected gives the highest antimicrobial activity and the best alcohol concentration using in the extraction of propolis , then the crude extract of propolis showed synergistic effect with some antibiotics in inhibition of pathogenic microorganisms.

Study of common aerobic flora of human cerumen

1998 ◽  
Vol 112 (7) ◽  
pp. 613-616 ◽  
Author(s):  
A. Campos ◽  
A. Arias ◽  
L. Betancor ◽  
C. Rodríguez ◽  
A. M. Hernández ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
External Auditory Canal ◽  
Staphylococcus Epidermidis ◽  
Pseudomonas Stutzeri ◽  
Climatic Conditions ◽  
Apocrine Glands ◽  
The Mean ◽  
Aerobic Flora

AbstractCerumen is the product of the secretion of the sebaceous, ceruminous or apocrine glands together with cells exfoliated from the cornified stratum of the epithelium of the external auditory canal (EAC).In the present study we identified and quantified common flora of human cerumen. The mean count obtained was 106 microorganisms per ml of cerumen suspension.In 24 pools of cerumen (33.3 per cent) the isolates were monomicrobial, Staphylococcus epidermidis (12), Corynebacterium spp (10), Staphylococcus aureus (1) and Streptococcus saprophyticum (1). In 48 pools (66.6 per cent) we found polymicrobial isolates.The most commonly isolated bacteria in these polymicrobial isolates were S. epidermidis (35) and Corynebacterium spp. (43). It is noteworthy that there were isolates of Candida albicans in three cases; in one case of Pseudomonas stutzeri, in one case of Pseudomonas aeruginosa, and, on seven occasions, of S. aureus.The organisms isolated as common bacterial components of human cerumen in our experience were similar to those found by other authors. However, the mean count was much higher. This could be related t o climatic conditions and to the length of time the cerumen had remained in the external auditory canal.

scholarly journals JONIZUOTO RŪGŠTINIO VANDENS ANTIMIKROBINIS AKTYVUMAS

2019 ◽  
Vol 28 (6) ◽  
pp. 64-68
Author(s):  
Albina Vaičiulevičienė ◽  
Goda Jonaitytė ◽  
Vaida Batulevičienė
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis ◽  
Proteus Vulgaris ◽  
Esherichia Coli

Užkrečiamųjų ligų ir AIDS centro (ULAC) teigimu, „Dezinfekcija yra viena iš užkrečiamųjų ligų perda­vimo rizikos mažinimo priemonių“ (2014). Jonizuotas rūgštinis vanduo pasirenkamas kaip mažiau toksiška žmgaus sveikatai ir aplinkai antimikrobinį poveikį tu­rinti medžiaga. Lietuvos UAB „Burbuliukas“ gamin­tojai siūlo alternatyvą cheminiams dezinfektantams - jonizuotą rūgštinį vandenį (anolitą), kuris saugus žmogui ir aplinkai bei pigesnis už cheminius dezin­fektantus ir naikina patogeninius mikroorganizmus. Tad šio darbo tikslas buvo įvertinti jonizuoto rūgšti­nio vandens antimikrobinį poveikį. Kauno kolegijos Medicinos fakulteto mikrobiologijos laboratorijoje UAB „Burbuliukas“ užsakymu buvo atliktas mikro­biologinis jonizuoto rūgštinio vandens (anolito) an­timikrobinis tyrimas plovinių metodu. Tirtas anolito poveikis užkrėstų paviršių (stalviršių, keramikinių plytelių, grindų linoleumo dangos) ir medicininių įrankių (stiklinių, keramikinių, metalinių) mikroor­ganizmais, turinčiais skirtingas biologines savybes. Tyrimui naudotos etaloninės mikroorganizmų kul­tūros, gautos OXOID Nr. 605068: Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Enterococcus faecalis ATCC 29212, Esherichia coli ATCC 25922, Klebsiella pneumo­niae ATCC 31488, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus subtilis ATCC6633, Candida albicans ATCC10231. Anolito ekspozicijos poveikis truko 15-30 min. Medicininiai instrumentai buvo pamerkti į anolitą, o paviršiai plau­nami anolite įmirkyta šluoste. Jonizuotas rūgštinis vanduo biocidiškai veikė skirtingas biologines savy­bes turinčius mikroorganizmus, kuriais buvo užkrėsti medicininiai instrumentai. Užkrėtus etaloninėmis mi­kroorganizmų kultūromis paviršius ir nuvalius anolitu du kartus su 15 minučių intervalu, po pirmo valymo aptiktos pavienės mikroorganizmų kolonijos, nuvalius antrą kartą - jonizuotas rūgštinis vanduo sunaikino sporines, nesporines bakterijas bei Candida grybus.

scholarly journals The ineffectiveness of antimicrobial photodynamic therapy in the absence of preincubation of the microorganisms in the photosensitizer

2020 ◽  
Vol 33 ◽  
Author(s):  
Ítalo Dany Cavalcante Galo ◽  
Jéssica Assis Carvalho ◽  
Jessyca Luana Melo Costa Santos ◽  
Alexandre Braoios ◽  
Rodrigo Paschoal Prado
Keyword(s):  
Staphylococcus Aureus ◽  
Methylene Blue ◽  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
Laser Emission ◽  
Alternative Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Essential Factor ◽  
Multiresistant Bacteria ◽  
Important Objective

Abstract Introduction: Considering its potential as an alternative therapy to combat multiresistant bacteria, photodynamic therapy has been improved and better studied in recent years, and determining its optimized application patterns is important. Objective: This study aimed to evaluate the action of antimicrobial photodynamic therapy mediated by methylene blue in the absence of preincubation of infectious agents in the photosensitizer. Method: Standard strains of Staphylococcus aureus and Pseudomonas aeruginosa were used, which was or was not submitted to two methylene blue concentrations (0.1 μg/mL and 500 mg/mL) applied alone or in combination with a variety of red laser emission parameters (660 nm); in both cases, the streak was performed immediately after mixing between the photosensitizer and the solution containing the bacteria. Results: In the dishes with only methylene blue application neither antibacterial was produced, nor inhibition at the application points of the photodynamic therapy in the case of the bacterium Pseudomonas aeruginosa. However, in the cultures of Staphylococcus aureus in which laser emission was associated with the concentration of 500 mg/mL of the photosensitizer, inhibition was present at the laser application points. Conclusion: The time of exposure to the photosensitizer prior to the application of phototherapy seems to be an essential factor for the optimized action of photodynamic therapy, especially in the case of Gram-negative bacteria.

scholarly journals SKIRTINGOSE LIETUVOS VIETOVĖSE SURINKTŲ ŽIEDADULKIŲ EKSTRAKTO ANTIMIKROBINIS VEIKIMAS

2021 ◽  
Vol 31 (7) ◽  
pp. 86-90
Author(s):  
Žaneta Maželienė ◽  
Gintarė Lingytė ◽  
Evelina Dailidaitė ◽  
Ingrida Viliušienė
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Listeria Monocytogenes ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis ◽  
Proteus Vulgaris ◽  
Microsoft Excel

Įvadas. Pasaulio sveikatos organizacijos (PSO) duomenimis, bakterijų atsparumas antibiotikams kelia didelę grėsmę visame pasaulyje ir sunkina infekcinių ligų gydymą [1]. Manoma, kad apie 70 proc. bakterijų, kurios sukelia infekcinius susirgimus ligoninėse, yra atsparios bent vienam iš gydymui naudojamų antibiotikų. Ši situacija skatina ieškoti kitų sprendimų, vienas iš jų – bičių produktus naudoti kaip natūralius antibiotikus dėl jų antimikrobinio veikimo. Tyrimo tikslas – nustatyti skirtingose Lietuvos vietovėse surinktų žiedadulkių ekstraktų antimikrobinį veikimą. Tyrimo metodika. Atliktas įvairiuose Lietuvos ūkiuose surinktų žiedadulkių skirtingos koncentracijos ekstraktų mikrobiologinis tyrimas. Nustatytas 50 žiedadulkių ekstraktų mėginių antimikrobinis poveikis dešimties etaloninių bakterijų kultūrų ir grybelio Candida albicans atžvilgiu. Tyrimo duomenų analizė atlikta naudojant aprašomąją statistiką, skaičiuojant dažnius bei grupių palyginimą pagal Kruskal Wallis, tyrimo duomenys apdoroti Microsoft Excel 2019 programa. Tyrimo rezultatai. Nustatyta, kad stipriausią antimikrobinį poveikį turėjo D žiedadulkių, surinktų Kaišiadorių rajone, ekstraktas. D ekstrakto antimikrobinis poveikis Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacillus cereus, Listeria monocytogenes atžvilgiu nustatytas esant 0,025 ml ekstrakto 1 agaro ml. Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Proteus vulgaris ir grybelį Candida albicans, išskyrus Escherichia coli, ekstraktas veikė esant jo 0,0375 – 0,05 ml agaro 1 ml. Žiedadulkių ekstraktas gramteigiamąsias bakterijas veikia stipriau, lyginant su gramneigiamosiomis. Šis skirtumas siejamas su gramteigiamų ir gramneigiamų bakterijų ląstelės sienelės struktūros skirtumais. Išvados. Stipriausią antimikrobinį veikimą turi žiedadulkės, surinktos Kaišiadorių rajone. Nustatyta, kad žiedadulkių ekstraktai stipriau veikia gramteigiamąsias bakterijas, lyginant su gramneigiamosiomis.

Use of internally validated in vitro biofilm models to assess antibiofilm performance of silver-containing gelling fibre dressings

2020 ◽  
Vol 29 (3) ◽  
pp. 154-161 ◽  
Author(s):  
Louise Suleman ◽  
Liam Purcell ◽  
Hannah Thomas ◽  
Samantha Westgate
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Antimicrobial Properties ◽  
Single Species ◽  
In Vitro Models ◽  
Wound Dressings ◽  
In Vitro Test ◽  
Multispecies Biofilms

Objective: To assess the efficacy of five silver-containing gelling fibre wound dressings against single-species and multispecies biofilms using internally validated, UKAS-accredited in vitro test models. Method: Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans single- and multispecies biofilms were cultured using Centres for Disease Control (CDC) biofilm reactors and colony drip flow reactors (CDFR). Following a 72 hour incubation period, the substrates on which biofilms were grown were rinsed to remove planktonic microorganisms and then challenged with fully hydrated silver-containing gelling fibre wound dressings. Following dressing application for 24 or 72 hours, remaining viable organisms from the treated biofilms were quantified. Results: In single-species in vitro models, all five antimicrobial dressings were effective in eradicating Staphylococcus aureus and Pseudomonas aeruginosa biofilm bacteria. However, only one of the five dressings (Hydrofiber technology with combination antibiofilm/antimicrobial technology) was able to eradicate the more tolerant single-species Candida albicans biofilm. In a more complex and stringent CDFR biofilm model, the hydrofiber dressing with combined antibiofilm/antimicrobial technology was the only dressing that was able to eradicate multispecies biofilms such that no viable organisms were recovered. Conclusion: Given the detrimental effects of biofilm on wound healing, stringent in vitro biofilm models are increasingly required to investigate the efficacy of antimicrobial dressings. Using accredited in vitro biofilm models of increasing complexity, differentiation in the performance of dressings with combined antibiofilm/antimicrobial technology against those with antimicrobial properties alone, was demonstrated.

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