scholarly journals Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix

RSC Advances ◽  
2019 ◽  
Vol 9 (61) ◽  
pp. 35695-35705 ◽  
Author(s):  
Anne Clasen ◽  
Sarah Wenderoth ◽  
Isabella Tavernaro ◽  
Jana Fleddermann ◽  
Annette Kraegeloh ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Ph Sensitive ◽  
Silica Matrix ◽  
Sensitive Indicator ◽  
Fluorescent Nanoparticles ◽  
Indicator Dye ◽  
Prompt Response

Self-calibrating, fluorescent nanoparticles with diameter far below 50 nm are synthesized with embedding a new ratiometric and pH sensitive indicator dye. The prompt response of the fluorophore allows for determining the intracellular pH.

Correction: Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple

BIO-PROTOCOL ◽  
2018 ◽  
Vol 8 (8) ◽  
Author(s):  
Aparecida Silva ◽  
Keini Dressano ◽  
Paulo Ceciliato ◽  
Juan Carlos Guerrero-Abad ◽  
Daniel Moura
Keyword(s):  
Ph Sensitive ◽  
Sensitive Indicator ◽  
Bromocresol Purple ◽  
Ph Change

LONG TERM EFFECTS OF THYROID STIMULATING HORMONE AND INSULIN ON INTRACELLULAR pH IN FRTL-5 CELLS

1992 ◽  
Vol 133 (2) ◽  
pp. R9-R11
Author(s):  
A.M. Wood ◽  
S.P. Bidey ◽  
J. Soden ◽  
W.R. Robertson
Keyword(s):  
Intracellular Ph ◽  
Small Groups ◽  
Thyroid Stimulating Hormone ◽  
Ph Sensitive ◽  
Long Term Effects ◽  
Bicarbonate Ions ◽  
Chronic Effects ◽  
Petri Dishes ◽  
Presence And Absence

ABSTRACT We have studied the chronic effects of TSH (100μU/ml) and insulin (10μg/ml) on intracellular pH (pHi) in FRTL-5 cells using the pH sensitive probe 2′7-bis (2-carboxyethyl-5′-6′) carboxyfluorescein. FRTL-5 cells were cultured on Petri dishes either in the presence of 4H, ie. Coons F-12 containing cortisol (10nM), transferrin (0.5μg/ml), glycyl-histidyl lysine acetate (10ng/ml) and somatostatin (10μg/ml), or with 4H+insulin (5H), 4H+TSH, or 4H+TSH+insulin (6H). pHi was measured in small groups of cells by microspectrofluorimetry both in the presence and absence of bicarbonate ions after cells had been deprived of serum for at least a day. In

Intracellular pH-Sensitive PEG-block-Acetalated-Dextrans as Efficient Drug Delivery Platforms

2013 ◽  
Vol 5 (21) ◽  
pp. 10760-10766 ◽  
Author(s):  
Zhe Zhang ◽  
Xiaofei Chen ◽  
Li Chen ◽  
Shuangjiang Yu ◽  
Yue Cao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Intracellular Ph ◽  
Ph Sensitive

Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles

2017 ◽  
Vol 46 (40) ◽  
pp. 13957-13965 ◽  
Author(s):  
Shuoren Du ◽  
Javier Hernández-Gil ◽  
Hao Dong ◽  
Xiaoyu Zheng ◽  
Guangming Lyu ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Quantitative Imaging ◽  
Living Cells ◽  
Ph Sensitive ◽  
Upconversion Nanoparticles ◽  
Imaging Probe ◽  
Ph Imaging ◽  
Ratiometric Probe ◽  
Subcellular Level

A ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells.

Intracellular pH measurements in gastric mucosa.

1979 ◽  
Vol 237 (1) ◽  
pp. E82
Author(s):  
S J Hersey
Keyword(s):  
Gastric Mucosa ◽  
Acid Secretion ◽  
Intracellular Ph ◽  
Ph Indicator ◽  
Active Secretion ◽  
Ph Measurements ◽  
Tubular Cells ◽  
A Value ◽  
Indicator Dye ◽  
Secretion Rates

Intracellular pH was measured in bullfrog gastric mucosa using a pH-indicator dye, bromthymol blue (BTB), with a spectrophotometric technique. Studies showed that BTB is taken up by the gastric mucosa and bound to intracellular components. The binding of BTB was shown to cause a shift in the pKa of the dye from the solution value of 6.95 to a value of 8.0. During the nonsecreting state, intracellular pH was estimated to be 7.4 (metiamide inhibition) or 7.1 (SCN inhibition). During active secretion of acid, intracellular pH increased with increasing secretion rates, reaching values in excess of pH 8. Using preparations from which the surface epithelial cells had been removed, it was shown that at least a portion of the alkaline response to stimulation occurs in the oxyntic or tubular cells. The results are interpreted in view of existing models for the chemical reaction involved in gastric acid secretion.

Regulation of intracellular pH in proximal tubules of avian long-looped mammalian-type nephrons

1998 ◽  
Vol 274 (6) ◽  
pp. R1526-R1535 ◽  
Author(s):  
Olga H. Brokl ◽  
Christina L. Martinez ◽  
Apichai Shuprisha ◽  
Diane E. Abbott ◽  
William H. Dantzler
Keyword(s):  
Intracellular Ph ◽  
Ph Sensitive ◽  
Fluorescent Dye ◽  
Proximal Tubules ◽  
Acid Base ◽  
Rate Of Recovery

In nonperfused proximal tubules isolated from chicken long-looped mammalian-type nephrons, intracellular pH (pHi), measured with the pH-sensitive fluorescent dye 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, was ∼7.3 under control conditions (HEPES-buffered medium with pH 7.4 at 37°C) and was reduced to ∼7.0 in response to NH4Cl pulse. The rate of recovery of pHi from this level to the resting level was 1) significantly reduced by the removal of Na+ from the bath, 2) significantly increased by the removal of Cl− from the bath, 3) unchanged by the removal of both Na+ and Cl− from the bath, 4) significantly reduced by the addition of either ethylisopropylamiloride or DIDS to the bath, 5) significantly increased by a high bath K+ concentration, and 6) unchanged by the addition of Ba2+ to the bath. These data suggest that both Na+-coupled and Cl−-coupled basolateral acid-base fluxes are involved in determining the rate of recovery of pHi after acidification. The most likely ones to be important in regulating pHi are a Na+/H+exchanger and a Na+-coupled Cl−/[Formula: see text]exchanger. In birds, long-looped mammalian-type nephrons resemble short-looped transitional nephrons but differ markedly from superficial loopless reptilian-type nephrons.

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