scholarly journals Target-activated transcription for the amplified sensing of protease biomarkers

2020 ◽  
Vol 11 (11) ◽  
pp. 2993-2998 ◽  
Author(s):  
Fang Liu ◽  
Min Yang ◽  
Wenlu Song ◽  
Xingyu Luo ◽  
Rui Tang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Amplification Strategy

A transcription-inspired signal amplification strategy that amplifies proteolysis into nucleic acid outputs is presented for the ultrasensitive sensing of protease biomarkers.

A novel electrochemical biosensor for ultrasensitive Hg2+ detection via a triple signal amplification strategy

2021 ◽  
Author(s):  
Wang He ◽  
Bin Qiao ◽  
Fengzhen Li ◽  
Lisha Pan ◽  
Delun Chen ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Amplification Strategy

We report a novel electrochemical biosensor for the ultrasensitive Hg2+ detection via a signal amplification strategy. This strategy could be easily modified and extended to detect other hazardous heavy metals and nucleic acid.

Recent advance in exonuclease Ⅲ-assisted target signal amplification strategy for nucleic acid detection

2021 ◽  
Author(s):  
hongyu liu ◽  
Yuhao You ◽  
Youzhuo Zhu ◽  
Heng Zheng
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Molecular Diagnostics ◽  
Mutation Analysis ◽  
Signal Amplification ◽  
Nucleic Acid Detection ◽  
Target Signal ◽  
Forensic Investigations ◽  
Exo Iii ◽  
Amplification Strategy

Detection of nucleic acids have become significantly important in molecular diagnostics, genetics therapy, mutation analysis, forensic investigations and biomedical development, and so on. In recent years, exonuclease Ⅲ (Exo III)...

An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection

2018 ◽  
Vol 54 (77) ◽  
pp. 10897-10900 ◽  
Author(s):  
Zhi-Bin Wen ◽  
Wen-Bin Liang ◽  
Ying Zhuo ◽  
Cheng-Yi Xiong ◽  
Ying-Ning Zheng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21.

Nucleic acid tool enzymes-aided signal amplification strategy for biochemical analysis: status and challenges

2015 ◽  
Vol 408 (11) ◽  
pp. 2793-2811 ◽  
Author(s):  
Taiping Qing ◽  
Dinggeng He ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Fengzhou Xu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Biochemical Analysis ◽  
Amplification Strategy

Isothermal cross-boosting extension–nicking reaction mediated exponential signal amplification for ultrasensitive detection of polynucleotide kinase

The Analyst ◽  
2020 ◽  
Vol 145 (10) ◽  
pp. 3742-3748 ◽  
Author(s):  
Xiao-Yu Li ◽  
Yun-Xi Cui ◽  
Yi-Chen Du ◽  
An-Na Tang ◽  
De-Ming Kong
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A novel nucleic acid-based isothermal signal amplification strategy, named cross-boosting extension–nicking reaction (CBENR) is developed and successfully used for rapid and ultrasensitive detection of polynucleotide kinase (PNK) activity.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Naked-Eye Detection of Hg(II) Ions by Visible Light-Induced Polymerization Initiated by a Hg(II)-Selective Photoredox Catalyst

2021 ◽  
Author(s):  
Hyungwook Kim ◽  
Young Jae Jung ◽  
Jungkyu K. Lee
Keyword(s):  
Visible Light ◽  
Signal Amplification ◽  
Eye Detection ◽  
Naked Eye ◽  
Turn On ◽  
Novel Strategy ◽  
Amplification Strategy ◽  
Naked Eye Detection

We developed a novel strategy for signal amplification strategy using a visible light-induced photopolymerization, initiated by a selective turn-on photoredox catalyst. As photoredox catalysts, fluorescein derivatives are able to initiate...

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