A “sandwich” cell culture platform with NIR-responsive dynamic stiffness to modulate macrophage phenotypes

2021 ◽  
Author(s):  
Peiqi Yuan ◽  
Yilun Luo ◽  
Yu Luo ◽  
Lie Ma
Keyword(s):  
Cell Culture ◽  
Near Infrared ◽  
Dynamic Stiffness ◽  
Phenotypic Transformation ◽  
Macrophage Phenotypes

A “sandwich” cell culture platform with the ability to be rapidly transformed from lower stiffness to higher stiffness under near-infrared (NIR) irradiation and to induce the phenotypic transformation from M2 to M1 sequentially.

scholarly journals Application of near-infrared spectroscopy for monitoring and control of cell culture and fermentation

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Albert E. Cervera ◽  
Nanna Petersen ◽  
Anna Eliasson Lantz ◽  
Anders Larsen ◽  
Krist V. Gernaey
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Monitoring And Control ◽  
And Control

Near-Infrared-Triggered Dynamic Surface Topography for Sequential Modulation of Macrophage Phenotypes

2019 ◽  
Vol 11 (46) ◽  
pp. 43689-43697 ◽  
Author(s):  
Xiaowen Zheng ◽  
Liaobing Xin ◽  
Yilun Luo ◽  
Huang Yang ◽  
Xingyao Ye ◽  
...  
Keyword(s):  
Surface Topography ◽  
Near Infrared ◽  
Dynamic Surface ◽  
Sequential Modulation ◽  
Macrophage Phenotypes

scholarly journals Novel approach for the prediction of cell densities and viability in standardized translucent cell culture biochips with near infrared spectroscopy

2017 ◽  
Vol 17 (5) ◽  
pp. 585-593 ◽  
Author(s):  
Marko Gröger ◽  
Matthias Lange ◽  
Knut Rennert ◽  
Tobias Kaschowitz ◽  
Holger Plettenberg ◽  
...  
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Novel Approach ◽  
Cell Densities

Abstract 591: Development of Near-infrared Fluorescent Probe for Targeted Uptake by Atherosclerotic Plaque Macrophages

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Marten Maess ◽  
Kosuke Shimizu ◽  
Yudai Narita ◽  
Naoto Oku ◽  
Yasuhiro Magata ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fluorescent Probe ◽  
Macaca Fascicularis ◽  
Near Infrared ◽  
Imaging Techniques ◽  
Macrophage Polarization ◽  
Marker Genes ◽  
Atherosclerotic Plaques ◽  
Monocytic Cell ◽  
Monocytic Cell Line

Macrophages are a pivotal element in the development of atherosclerotic plaques and their polarization strongly affects plaque stability. Development of molecular imaging techniques to identify vulnerable plaques in atherosclerotic vessel walls will be a valuable diagnostic tool to assess patients` risk of myocardial infarction or stroke and to adjust therapy. Imaging of unstable plaques has so far been performed by using PET imaging agent [ 18 F] FDG. Fluorescence imaging using near-infrared fluorescent agents offers several advantages, as they are simple to use and minimally invasive. We have developed an activable near-infrared fluorescent probe using either peptide-linked ICG dye (indocyanine green) or free ICG as cargo in liposomes. The liposomes were labeled with phosphatidyl serine in order to trigger phagocytic uptake by macrophages. We have investigated the properties of our liposome probes in order to assess their suitability for screening of atherosclerosis risk patients in a wide array of different experimental models including cell culture and various animals (apoE -/- mice, WHHL rabbits and macaca fascicularis monkeys). Cell culture experiments using the human monocytic cell line THP-1 after differentiation into macrophages using the phorbol ester PMA and polarization of the macrophages by either LPS/IFNγ for M1 polarization or IL-4/ IL-13 for M2 polarization have shown considerable differences in uptake efficiency for the liposomes. M2 macrophages were found to show highest phagocytic activity while not polarized M0 were intermediate and M1 macrophages were least active. Polarization was confirmed by measuring known marker genes (TNFA, CCL3, MRC1) of macrophage polarization by RT-qPCR. In the animal studies we observed enrichment of ICG-labeled liposomes in atherosclerotic plaques of apoE -/- mice as well as WHHL rabbits. Macaca fascicularis experiments attempted to simulate final application in human subjects by detecting atherosclerotic plaques within the carotid aorta. Preliminary results indicate promising potential for liposome probes.

scholarly journals Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1765 ◽  
Author(s):  
Dedy Septiadi ◽  
Laura Rodriguez-Lorenzo ◽  
Sandor Balog ◽  
Miguel Spuch-Calvar ◽  
Giovanni Spiaggia ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colloidal Stability ◽  
Near Infrared ◽  
Culture Media ◽  
Nir Spectroscopy ◽  
Lung Epithelial Cells ◽  
Adherent Cell ◽  
Adherent Cell Culture ◽  
Protein Rich

The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose–response relationship is still a challenge due to the analytical difficulty of quantifying the dose delivered to cells. An approach to accurately quantify CNT doses for submerged in vitro adherent cell culture systems using UV-VIS-near-infrared (NIR) spectroscopy is provided here. Two types of multi-walled CNTs (MWCNTs), Mitsui-7 and Nanocyl, which are dispersed in protein rich cell culture media, are studied as tested materials. Post 48 h of CNT incubation, the cellular fractions are subjected to microwave-assisted acid digestion/oxidation treatment, which eliminates biological matrix interference and improves CNT colloidal stability. The retrieved oxidized CNTs are analyzed and quantified using UV-VIS-NIR spectroscopy. In vitro imaging and quantification data in the presence of human lung epithelial cells (A549) confirm that up to 85% of Mitsui-7 and 48% for Nanocyl sediment interact (either through internalization or adherence) with cells during the 48 h of incubation. This finding is further confirmed using a sedimentation approach to estimate the delivered dose by measuring the depletion profile of the CNTs.

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