scholarly journals Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1765 ◽  
Author(s):  
Dedy Septiadi ◽  
Laura Rodriguez-Lorenzo ◽  
Sandor Balog ◽  
Miguel Spuch-Calvar ◽  
Giovanni Spiaggia ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colloidal Stability ◽  
Near Infrared ◽  
Culture Media ◽  
Nir Spectroscopy ◽  
Lung Epithelial Cells ◽  
Adherent Cell ◽  
Adherent Cell Culture ◽  
Protein Rich

The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose–response relationship is still a challenge due to the analytical difficulty of quantifying the dose delivered to cells. An approach to accurately quantify CNT doses for submerged in vitro adherent cell culture systems using UV-VIS-near-infrared (NIR) spectroscopy is provided here. Two types of multi-walled CNTs (MWCNTs), Mitsui-7 and Nanocyl, which are dispersed in protein rich cell culture media, are studied as tested materials. Post 48 h of CNT incubation, the cellular fractions are subjected to microwave-assisted acid digestion/oxidation treatment, which eliminates biological matrix interference and improves CNT colloidal stability. The retrieved oxidized CNTs are analyzed and quantified using UV-VIS-NIR spectroscopy. In vitro imaging and quantification data in the presence of human lung epithelial cells (A549) confirm that up to 85% of Mitsui-7 and 48% for Nanocyl sediment interact (either through internalization or adherence) with cells during the 48 h of incubation. This finding is further confirmed using a sedimentation approach to estimate the delivered dose by measuring the depletion profile of the CNTs.

scholarly journals Smart Shockwave Responsive Titania-Based Nanoparticles for Cancer Treatment

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1423
Author(s):  
Veronica Vighetto ◽  
Luisa Racca ◽  
Marta Canta ◽  
Joana C. Matos ◽  
Bianca Dumontel ◽  
...  
Keyword(s):  
Cell Culture ◽  
Shock Waves ◽  
Cancer Cell ◽  
Colloidal Stability ◽  
Culture Media ◽  
High Sensitivity ◽  
High Energy ◽  
High Dispersion ◽  
Specific Chemical

Nanomedicine is an emerging treatment approach for many cancers, characterized by having high sensitivity and selectivity for tumor cells and minimal toxic effects induced by the conventional chemotherapeutics. In these context, smart nanoparticles (NPs) are getting increasingly relevant in the development of new therapies. NPs with specific chemical composition and/or structure and being stimuli-responsive to magnetic, light or ultrasound waves are new promising tools. In the present work, amorphous-titania propyl-amine functionalized (a-TiO2-NH2) NPs, coated with bovine serum albumin (BSA), are stimulated with high energy shock waves to induce cytotoxic effects in cancer cells. First, a new method to coat a-TiO2-NH2 NPs with BSA (a-TiO2-NH2/BSA) was proposed, allowing for a high dispersion and colloidal stability in a cell culture media. The a-TiO2-NH2/BSA NPs showed no cancer cell cytotoxicity. In a second step, the use of shock waves to stimulate a-TiO2-NH2/BSA NPs, was evaluated and optimized. A systematic study was performed in in vitro cell culture aiming to impair the cancer cell viability: NP concentrations, time steps and single versus multiple shock waves treatments were studied. The obtained results highlighted the relevance of NPs design and administration time point with respect to the shock wave treatment and allow to hypothesize mechanical damages to cells.

scholarly journals Induction of murine malignant lymphoma by 1-(2-chloro-ethyl)-3-cyclo-hexyl-1-nitrosourea, In vitro partial characterization

2013 ◽  
Vol 7 (1) ◽  
pp. 61-66
Author(s):  
Ahmed M. Al-Shammari
Keyword(s):  
Cell Culture ◽  
Malignant Lymphoma ◽  
Cell Line ◽  
Cultured Cells ◽  
Partial Characterization ◽  
Adherent Cell ◽  
Short Term ◽  
Adherent Cell Culture ◽  
Short Term Culture

From 20 mice administered ethyl nitrosourea for over 5 months, 2 mice showed lung lymphoma, one of them is a murine malignant lymphoma which cultured and the cell line was newly established as short term culture. The cells were round in shape and had a tendency to make groups of floating clusters. Later these cells developed to proliferate as adherent cell culture. Our study showed that ethyl nitrosourea is selective carcinogen to induce lymphoma and lymphoma cultured cells is very useful for lymphoma studies.

scholarly journals Use of a non-adherent cell culture system for testing the effect of 2′,3′-dideoxyinosine againstCryptosporidium parvum

1999 ◽  
Vol 176 (1) ◽  
pp. 235-240
Author(s):  
Philippe Lawton ◽  
Carine Hejl ◽  
Marie-Elisabeth Sarciron ◽  
Roselyne Mancassola ◽  
Muriel Naciri ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Adherent Cell ◽  
Cell Culture System ◽  
Adherent Cell Culture

scholarly journals Impact of serum in cell culture media on in vitro lactate dehydrogenase (LDH) release determination

2017 ◽  
Vol 3 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Bernhard Hiebl ◽  
Sinem Peters ◽  
Ole Gemeinhardt ◽  
Stefan M. Niehues ◽  
Friedrich Jung
Keyword(s):  
Cell Culture ◽  
Lactate Dehydrogenase ◽  
Culture Media ◽  
Cell Culture Media ◽  
Ldh Release

scholarly journals A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine

2021 ◽  
Vol 22 (18) ◽  
pp. 9896
Author(s):  
Eugenia Romano ◽  
Paolo Antonio Netti ◽  
Enza Torino
Keyword(s):  
Cell Culture ◽  
High Pressure ◽  
Culture Media ◽  
Preliminary Test ◽  
Bilayer Membrane ◽  
Microfluidic System ◽  
Culture Model ◽  
Before And After ◽  
Dynamic High Pressure

In recent decades, endogenous nanocarrier-exosomes have received considerable scientific interest as drug delivery systems. The unique proteo-lipid architecture allows the crossing of various natural barriers and protects exosomes cargo from degradation in the bloodstream. However, the presence of this bilayer membrane as well as their endogenous content make loading of exogenous molecules challenging. In the present work, we will investigate how to promote the manipulation of vesicles curvature by a high-pressure microfluidic system as a ground-breaking method for exosomes encapsulation. Exosomes isolated from Uppsala 87 Malignant Glioma (U87-MG) cell culture media were characterized before and after the treatment with high-pressure homogenization. Once their structural and biological stability were validated, we applied this novel method for the encapsulation in the lipidic exosomal bilayer of the chemotherapeutic Irinotecan HCl Trihydrate-CPT 11. Finally, we performed in vitro preliminary test to validate the nanobiointeraction of exosomes, uptake mechanisms, and cytotoxic effect in cell culture model.

NanoPADs and nanoFACEs: an optically transparent nanopaper-based device for biomedical applications

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3322-3333
Author(s):  
Binbin Ying ◽  
Siwan Park ◽  
Longyan Chen ◽  
Xianke Dong ◽  
Edmond W. K. Young ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microfluidic Device ◽  
Biomedical Applications ◽  
Adherent Cell ◽  
Optically Transparent ◽  
Adherent Cell Culture

A highly transparent nanopaper-based microfluidic device for chemical/biosensing and cell culture, which is branded as nanopaper-based analytical devices (nanoPADs) and nanofibrillated adherent cell-culture platforms (nanoFACEs).

Near infrared (NIR)-spectroscopy and in-vitro dissolution absorption system 2 (IDAS2) can help detect changes in the quality of generic drugs

2020 ◽  
Vol 46 (1) ◽  
pp. 80-90
Author(s):  
Carlos Jiménez-Romero ◽  
Johayra Simithy ◽  
Anthony Severdia ◽  
Daniel Álvarez ◽  
Manuel Grosso ◽  
...  
Keyword(s):  
Near Infrared ◽  
Generic Drugs ◽  
Nir Spectroscopy ◽  
In Vitro Dissolution ◽  
Absorption System ◽  
System 2

Triggered drug release from hybrid thermoresponsive nanoparticles using near infrared light

Nanomedicine ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 219-234 ◽  
Author(s):  
Isabel Ortiz de Solorzano ◽  
Martin Prieto ◽  
Gracia Mendoza ◽  
Victor Sebastian ◽  
Manuel Arruebo
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Colloidal Stability ◽  
Near Infrared ◽  
Chemical Characterization ◽  
Infrared Light ◽  
Galvanic Replacement ◽  
Heating Efficiency ◽  
Hollow Gold Nanoparticles

Aim: Developing hybrid poly(N-isopropylacrylamide)-based nanogels decorated with plasmonic hollow gold nanoparticles for on-demand drug delivery and their physico-chemical characterization, bupivacaine loading and release ability upon light irradiation, and in vitro cell viability. Materials & methods: Hollow gold nanoparticles were prepared by galvanic replacement reaction; poly(N-isopropylacrylamide)-based nanogels were synthesized via precipitation polymerization and their electrostatic coupling was accomplished using poly(allylamine hydrochloride) as cationic polyelectrolyte linker. Results & conclusion: Colloidal stability of the resulted hybrid nanovectors was demonstrated under physiological conditions together with their fast response and excellent heating efficiency after light stimulation, indicating their potential use as triggered drug-delivery vectors. Moreover, their influence on cell metabolism and cell cycle under subcytotoxic doses were studied showing excellent cytocompatibility.

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