Sonophotocatalysis-mediated morphological transition modulates virulence and antibiotic resistance in Salmonella Typhimurium

2020 ◽  
Vol 6 (7) ◽  
pp. 1917-1930
Author(s):  
A. P. Habeeb Rahman ◽  
Ananyo Jyoti Misra ◽  
Swagatika Panda ◽  
Bhaskar Das ◽  
Prachi Bhol ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antibiotic Resistance ◽  
Salmonella Typhimurium ◽  
Cell Formation ◽  
Morphological Transition

Oxidative stress induced by sublethal sonophotocatalysis causes spheroplast like cell formation and reduction in antibiotic resistance in Salmonella Typhimurium.

scholarly journals Decreased Antibiotic Susceptibility in Pseudomonas aeruginosa Surviving UV Irradition

2021 ◽  
Vol 12 ◽  
Author(s):  
Hai-bei Li ◽  
Ai-ming Hou ◽  
Tian-jiao Chen ◽  
Dong Yang ◽  
Zheng-shan Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Uv Irradiation ◽  
Antibiotic Susceptibility ◽  
Antibiotic Resistance Genes ◽  
Polymyxin B ◽  
Uv Exposure ◽  
Uv Disinfection ◽  
Excellent Performance

Given its excellent performance against the pathogens, UV disinfection has been applied broadly in different fields. However, only limited studies have comprehensively investigated the response of bacteria surviving UV irradiation to the environmental antibiotic stress. Here, we investigated the antibiotic susceptibility of Pseudomonas aeruginosa suffering from the UV irradiation. Our results revealed that UV exposure may decrease the susceptibility to tetracycline, ciprofloxacin, and polymyxin B in the survival P. aeruginosa. Mechanistically, UV exposure causes oxidative stress in P. aeruginosa and consequently induces dysregulation of genes contributed to the related antibiotic resistance genes. These results revealed that the insufficient ultraviolet radiation dose may result in the decreased antibiotic susceptibility in the pathogens, thus posing potential threats to the environment and human health.

​Optimization of Cultural Conditions for Maximum Production of Outer Membrane Vesicles from Salmonella Typhimurium

2021 ◽  
Author(s):  
Menguzotunuo Solo ◽  
Shantanu Tamuly ◽  
Luit Moni Barkalita ◽  
Girin Kumar Saikia ◽  
Dhruba Jyoti Kalita
Keyword(s):  
Oxidative Stress ◽  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Large Scale ◽  
Multiple Drug Resistance ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multiple Drug ◽  
Scale Production ◽  
Maximum Production

Background: The non-typhoidal Salmonella causes gastroenteritis in humans that makes its way to the food chain mainly through the animal products. The multiple drug resistance imposes one of the major hurdle in the treatment of the disease. The vaccination appears to be the most important method for prevention of the disease. Unfortunately, there is no liscenced vaccine available against non-typhoidal Salmonellae. The use of outer membrane vesicles (OMVs) of Salmonella as a vaccine candidate has attained significant centre-stage in the recent years given to its protective immunogenicity. However, the large scale production of OMVs is difficult owing to low yield per liter of culture. Methods: In the present study, we have optimized the culture conditions viz. pH, phase of growth and presence of oxidative stress for maximum production of OMVs from Salmonella Typhimurium. The OMVs were characterized based on yield based on protein concentration, lipopolysaccharide concentration and zeta size. Result: In the present study, it was found that incubation of Salmonella Typhimurium up to peak of the growth phase at pH 7 in presence of oxidative stress was found to be the most suitable condition for maximum production of OMVs.

scholarly journals Insights into the Periplasmic Proteins of Acinetobacter baumannii AB5075 and the Impact of Imipenem Exposure: A Proteomic Approach

2019 ◽  
Vol 20 (14) ◽  
pp. 3451 ◽  
Author(s):  
Scribano ◽  
Marzano ◽  
Mortera ◽  
Sarshar ◽  
Vernocchi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Protein Interactions ◽  
Biological Response ◽  
Bacterial Viability ◽  
Proteomic Approach ◽  
Periplasmic Proteins ◽  
General Response ◽  
The Impact

Carbapenem-resistant Acinetobacter baumannii strains cause life-threatening infections due to the lack of therapeutic options. Although the main mechanisms underlying antibiotic-resistance have been extensively studied, the general response to maintain bacterial viability under antibiotic exposure deserves to be fully investigated. Since the periplasmic space contains several proteins with crucial cellular functions, besides carbapenemases, we decided to study the periplasmic proteome of the multidrug-resistant (MDR) A. baumannii AB5075 strain, grown in the absence and presence of imipenem (IMP). Through the proteomic approach, 65 unique periplasmic proteins common in both growth conditions were identified: eight proteins involved in protein fate, response to oxidative stress, energy metabolism, antibiotic-resistance, were differentially expressed. Among them, ABUW_1746 and ABUW_2363 gene products presented the tetratricopeptide repeat motif, mediating protein-protein interactions. The expression switch of these proteins might determine specific protein interactions to better adapt to changing environmental conditions. ABUW_2868, encoding a heat shock protein likely involved in protection against oxidative stress, was upregulated in IMP-exposed bacteria. Accordingly, the addition of periplasmic proteins from A. baumannii cultured with IMP increased bacterial viability in an antioxidant activity assay. Overall, this study provides the first insights about the composition of the periplasmic proteins of a MDR A. baumannii strain, its biological response to IMP and suggests possible new targets to develop alternative antibiotic drugs.

Effects of In-Feed Egg Yolk Antibodies on Salmonella Shedding, Bacterial Antibiotic Resistance, and Health of Pigs

2009 ◽  
Vol 72 (2) ◽  
pp. 267-273 ◽  
Author(s):  
ALAN G. MATHEW ◽  
SUKANYA RATTANATABTIMTONG ◽  
CHARLES M. NYACHOTI ◽  
LIN FANG
Keyword(s):  
Antibiotic Resistance ◽  
Blood Cell ◽  
Salmonella Typhimurium ◽  
Plasma Concentrations ◽  
Egg Yolk ◽  
Treatment Groups ◽  
Cell Counts ◽  
Egg Yolk Antibodies ◽  
Blood Cell Counts ◽  
White Blood Cell Counts

To determine effects of anti-Salmonella egg yolk antibodies on shedding and antibiotic resistance of Salmonella enterica Typhimurium, newly weaned pigs were randomly assigned to six dietary treatments. These treatment groups were (i) a control (basal) diet without additives and similar diets with (ii) egg yolk powder derived from chickens challenged with Salmonella Typhimurium antigens, (iii) a commercial egg yolk powder control, (iv) apramycin for 14 days followed by carbadox, (v) oxytetracycline, or (vi) spray-dried plasma protein. Treatments were provided beginning on day 3 of the trial, and all pigs were challenged with 5 ml of 108 CFU/ml Salmonella Typhimurium on day 7. Fecal samples were collected on days 0, 7, 8, 12, 14, 21, 58, 88, and 118 for isolation of Salmonella and Escherichia coli to determine shedding and antibiotic resistance patterns. Body weights, white blood cell counts, and plasma concentrations of anti-Salmonella immunoglobulin G and porcine interleukin 1β were analyzed as indicators of animal health. The percentage of pigs shedding Salmonella was lower for antibiotic treatment groups compared with other groups; however, resistance was greater in E. coli from pigs fed antibiotics than in pigs in other treatment groups. Health and performance indicators (pig weight gains, white blood cell counts, and plasma concentrations of anti-Salmonella antibodies) did not differ between treatment groups. These studies indicate that feeding egg yolk containing anti-Salmonella immunoglobulin Y may not be effective in controlling shedding of Salmonella in pigs.

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