Development of elastic artificial vessels with a digital pulse flow system to investigate the risk of restenosis and vasospasm

Lab on a Chip ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 3051-3059
Author(s):  
Yalei Lv ◽  
Guoqiang Li ◽  
Hongyu Peng ◽  
Yanping Liu ◽  
Jingru Yao ◽  
...  
Keyword(s):  
Quantitative Study ◽  
Flow System ◽  
Pulse Flow ◽  
Digital Pulse ◽  
Research Platform

We constructed elastic artificial vessels with a digital pulse flow system, which provide an in vitro research platform that approaches the in vivo microenvironment and facilitates a quantitative study on investigating the risk of restenosis and vasospasm.

scholarly journals Multiangle light-scattering analysis of murine teratocarcinoma cells.

1979 ◽  
Vol 27 (1) ◽  
pp. 366-370 ◽  
Author(s):  
D E Swartzendruber ◽  
B J Price ◽  
L B Rall
Keyword(s):  
Stem Cells ◽  
Light Scattering ◽  
Flow System ◽  
Yolk Sac ◽  
Squamous Cells ◽  
Teratocarcinoma Cells ◽  
Visceral Yolk Sac ◽  
Yolk Sac Cells

Stem cells of the mouse testicular teratocarcinoma are capable of giving rise in vivo and in vitro to a wide variety of cell and tissue types representative of each embryonic germ layer. Multiangle light-scattering measurements in a flow system have been made on these stem cells and on a variety of their differentiated derivatives. This technique is capable of distinguishing the stem cells from parietal yolk sac cells, visceral yolk sac cells, neuronal cells and squamous cells. However, multipotential stem cells cannot be distinguished from stem cells that are restricted in their development to a single pathway.

Assessment of in vivo MR imaging compared to physical sections in vitro—A quantitative study of brain volumes using stereology

NeuroImage ◽  
2005 ◽  
Vol 26 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jacob Jelsing ◽  
Egill Rostrup ◽  
Karin Markenroth ◽  
Olaf B. Paulson ◽  
Hans Jørgen G. Gundersen ◽  
...  
Keyword(s):  
Mr Imaging ◽  
Quantitative Study ◽  
Brain Volumes

scholarly journals A quantitative study on the in vitro and in vivo acetylation of high mobility group A1 proteins

2007 ◽  
Vol 18 (9) ◽  
pp. 1569-1578 ◽  
Author(s):  
Qingchun Zhang ◽  
Kangling Zhang ◽  
Yan Zou ◽  
Avi Perna ◽  
Yinsheng Wang
Keyword(s):  
Quantitative Study ◽  
High Mobility ◽  
High Mobility Group

scholarly journals A Multipumping Flow System for In Vitro Screening of Peroxynitrite Scavengers

2007 ◽  
Vol 12 (6) ◽  
pp. 875-880 ◽  
Author(s):  
Marta F.T. Ribeiro ◽  
Ana C.B. Dias ◽  
Joao L.M. Santos ◽  
Eduarda Fernandes ◽  
José L.F.C. Lima ◽  
...  
Keyword(s):  
Flow System ◽  
Sodium Hydroxide Solution ◽  
Biological Molecules ◽  
Diffusion Reaction ◽  
Oxidized Glutathione ◽  
Pulsed Flow ◽  
Peroxynitrite Anion ◽  
Emission Detection

Peroxynitrite anion is a reactive nitrogen species formed in vivo by the rapid, controlled diffusion reaction between nitric oxide and superoxide radicals. By reacting with several biological molecules, peroxynitrite may cause important cellular and tissue deleterious effects, which have been associated with many diseases. In this work, an automated flow-based procedure for the in vitro generation of peroxynitrite and subsequent screening of the scavenging activity of selected compounds is developed. This procedure involves a multipumping flow system (MPFS) and exploits the ability of compounds such as lipoic acid, dihydrolipoic acid, cysteine, reduced glutathione, oxidized glutathione, sulindac, and sulindac sulfone to inhibit the chemiluminescent reaction of luminol with peroxynitrite under physiological simulated conditions. Peroxynitrite was generated in the MPFS by the online reaction of acidified hydrogen peroxide with nitrite, followed by a subsequent stabilization by merging with a sodium hydroxide solution to rapidly quench the developing reaction. The pulsed flow and the timed synchronized insertion of sample and reagent solutions provided by the MPFS ensure the establishment of the reaction zone only inside the flow cell, thus allowing maximum chemiluminescence emission detection. The results obtained for the assayed compounds show that, with the exception of oxidized glutathione, all are highly potent scavengers of peroxynitrite at the studied concentrations. ( Journal of Biomolecular Screening 2007:875-880)

Smear removal agents: A quantitative study in vivo and in vitro

1987 ◽  
Vol 57 (2) ◽  
pp. 174-179 ◽  
Author(s):  
S.D. Meryon ◽  
R.S. Tobias ◽  
K.J. Jakeman
Keyword(s):  
Quantitative Study

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Ultrastructure of melanocyte-keratinocyte interactions and pigment transfer in vitro

1978 ◽  
Vol 36 (2) ◽  
pp. 650-651
Author(s):  
Raul I. Garcia ◽  
Evelyn A. Flynn ◽  
George Szabo
Keyword(s):  
Direct Injection ◽  
Skin Pigmentation ◽  
Freeze Fracture ◽  
Pigment Granule ◽  
Time Lapse ◽  
Ultrastructural Level ◽  
Lanthanum Tracer ◽  
A Cell

Skin pigmentation in mammals involves the interaction of epidermal melanocytes and keratinocytes in the structural and functional unit known as the Epidermal Melanin Unit. Melanocytes(M) synthesize melanin within specialized membrane-bound organelles, the melanosome or pigment granule. These are subsequently transferred by way of M dendrites to keratinocytes(K) by a mechanism still to be clearly defined. Three different, though not necessarily mutually exclusive, mechanisms of melanosome transfer have been proposed: cytophagocytosis by K of M dendrite tips containing melanosomes, direct injection of melanosomes into the K cytoplasm through a cell-to-cell pore or communicating channel formed by localized fusion of M and K cell membranes, release of melanosomes into the extracellular space(ECS) by exocytosis followed by K uptake using conventional phagocytosis. Variability in methods of transfer has been noted both in vivo and in vitro and there is evidence in support of each transfer mechanism. We Have previously studied M-K interactions in vitro using time-lapse cinemicrography and in vivo at the ultrastructural level using lanthanum tracer and freeze-fracture.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

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