scholarly journals A rapid and ultrasensitive colorimetric biosensor based on aptamer functionalized Au nanoparticles for detection of saxitoxin

RSC Advances ◽  
2020 ◽  
Vol 10 (26) ◽  
pp. 15293-15298
Author(s):  
Le Qiang ◽  
Yu Zhang ◽  
Xin Guo ◽  
Yakun Gao ◽  
Yingkuan Han ◽  
...  

We demonstrate a facile and ultrasensitive colorimetric sensor based on gold nanoparticles (Au NPs) and aptamer (Au NPs-aptamer biosensor) for specific and quantitative detection of STX.

2020 ◽  
Vol 16 (8) ◽  
pp. 1296-1303
Author(s):  
Xiaojuan Xia ◽  
Mei Li ◽  
Min Wang ◽  
Meng-Qiao Gu ◽  
Kuan-Neng Chi ◽  
...  

Ochratoxin is teratogenic, carcinogenic and immunotoxic to humans. It contains ochratoxin in many foods, so the detection of ochratoxin in food is particularly important. In this paper, gold nanoparticles (Au NPs)@g-C3N4 composite was synthesized by loading Au NPs on carbon nitride material, and it was immobilized on the surface of glassy carbon electrode by chitosan (Chit) as the substrate of electrochemical aptasensors. An ochratomycin A electrochemical aptasensor was constructed by hybridizing DNA1 and ochratoxin A (OTA) aptamers. The resulting hybrid strands were immobilized on the substrate of glassy carbon electrode. Electrochemical alternating current impedance (EIS) was used to detect the impedance value of the aptasensor when incubating different concentrations of OTA. The impedance value is inversely ratio to the concentration of OTA, achieving quantitative detection of OTA. The aptasensor can detect OTA with a linear range of 0.5–100 ng/mL, the linear correlation coefficient is 0.9506, and the detection limit is 0.167 ng/mL. This aptasensor provides a novel and efficient method for detecting OTA.


Nanoscale ◽  
2021 ◽  
Author(s):  
Lixiang Xing ◽  
Cui Wang ◽  
Yi Cao ◽  
Jihui Zhang ◽  
Haibing Xia

In this work, macroscopical monolayer films of ordered arrays of gold nanoparticles (MMF-OA-Au NPs) are successfully prepared at the interfaces of toluene-diethylene glycol (DEG) with a water volume fraction of...


2018 ◽  
Vol 72 (11) ◽  
pp. 1645-1652 ◽  
Author(s):  
Kailong Dong ◽  
Jiasheng Zhou ◽  
Taiqun Yang ◽  
Shan Dai ◽  
Hao Tan ◽  
...  

In this study, we report a straightforward strategy for Hg2+ ion detection. Fluorescent Au nanoparticles (NPs) were one-pot synthesized using a polymer (polyvinyl pyrrolidone [PVP]) as both capping and fluorescence agent. The as-synthesized PVP-Au NPs showed a remarkably rapid response selectively for Hg2+ ions compared to 14 other metal ions. The detection limit of Hg2+ was estimated at 100 nM. We discuss the emission and quenching mechanism of the PVP-Au NPs, the former being attributed to metal enhanced fluorescence and the latter being related to static quenching by Hg2+. The fluorescence of PVP-Au NPs offers an efficient and reliable strategy for Hg2+ ions detection. They therefore have a great potential for applications in health and environmental monitoring.


RSC Advances ◽  
2015 ◽  
Vol 5 (57) ◽  
pp. 46240-46246 ◽  
Author(s):  
Mahmoud Nasrollahzadeh ◽  
S. Mohammad Sajadi

This paper reports on the green synthesis of gold nanoparticles (Au NPs) by Anthemis xylopoda flowers aqueous extract without the addition of surfactant agents and their application as stable catalysts for the synthesis of propargylamines.


2017 ◽  
Vol 8 (31) ◽  
pp. 4528-4537 ◽  
Author(s):  
Eunyong Seo ◽  
Sang-Ho Lee ◽  
Sangho Lee ◽  
Soo-Hyung Choi ◽  
Craig J. Hawker ◽  
...  

We herein report a facile synthetic method for preparing gold nanoparticles (Au NPs) with superior colloidal stability using a series of double hydrophilic block copolymers (DHBC), poly(ethylene oxide)-block-poly(acrylic acid) (PEO-b-PAA), as a template (Au@DHBC NPs).


2017 ◽  
Vol 9 (23) ◽  
pp. 3561-3568 ◽  
Author(s):  
Yan Zhang ◽  
Yi Zhang ◽  
Wujuan Chen ◽  
Yating Zhang ◽  
Luqi Zhu ◽  
...  

We reported the use of polydopamine/Au nanoparticles/DNA (PDA/Au NPs/DNA) as a stationary phase for the separation of tryptophan enantiomers by microchip capillary electrophoresis (MCE).


2021 ◽  
Vol 19 (7) ◽  
pp. 25-34
Author(s):  
Alaa A. Shanef ◽  
Waleed Hameed Abed ◽  
Ahmed R. Mathloom

This works strong the embossing as regards riches (Au) nanoparticles (NPs) regarding attenuate TiO2 films. Au (Au-TiO2) patterned flabby film TiO2. The morphology, cleanly structure, issue administration then optical homes concerning the films hold been studied. Gold nanoparticles (Au-NPs) are synthesized along the useful resource on lowering hydrated gold steel ions of touch together along aqueous sow dermis extract. The alternate among the elevation or assignment about Au NPs elevated the reply above the ethanol gas sensors. The organized Au-TiO2 was once tested, of assessment together with allusion TiO2 membranes, such then ethanol sensors at 250-350 ° C at a fuel attention of 50-1000 ppm. In addition, the SERS deep concerning the excised Au/ TiO2 emaciated movie is about iii cases that above the deposited Au/ TiO2 gaunt film. The results proven in imitation of so much aggregation Au-coated TiO2 at 6 s spray epoch gave the beneficial file on 514 at 350 ° C deed useless heat since 1000 ppm fuel concentration.


Author(s):  
Garncarek ◽  
Kowalska-Góralska ◽  
Senze ◽  
Czyż

Applications of nanotechnologies in different sectors and everyday items are very promising and their popularity continues to grow. The number of products containing nanoparticles makes environmental exposure to nanoparticles inevitable. The current understanding of the relationships between nanoparticles and the environment is inadequate despite the fast growth of nanotechnologies. The aim of the study was to investigate the influence of copper and gold nanoparticles on Daphnia pulex survival. Our study included 48-hour acute toxicity tests and determination of median lethal concentration values (LC50s) for Cu-NPs and Au-NPs. For nano-copper, 24 h LC50 was assumed > 1 mg/L, and 48 h LC50 = 0.5117 mg/L. For nano-gold the LC50 value after 24 h was 0.4027 mg/L, and after 48 h 0.1007 mg/L. The toxicity of nano -gold solutions was thus found to be higher than that of nano-copper. The addition of Cu-NPs at 0.0625 mg/L and 0.125 mg/L caused an increased multiplication of daphnia, while Au-NPs at 1 mg/L was an inhibitor of reproduction.


RSC Advances ◽  
2015 ◽  
Vol 5 (52) ◽  
pp. 42081-42087 ◽  
Author(s):  
Ramesh Raliya ◽  
Pratim Biswas

The synthesis and characterization of stable gold nanoparticles (Au NPs) from gold chloride in soluble protein extracts of tomato (Solanum lycopersicum L.) leaves is demonstrated.


2021 ◽  
Vol 22 (22) ◽  
pp. 12155
Author(s):  
Mariapompea Cutroneo ◽  
Vladimir Havranek ◽  
Anna Mackova ◽  
Petr Malinsky ◽  
Letteria Silipigni ◽  
...  

Typically, polymeric composites containing nanoparticles are realized by incorporating pre-made nanoparticles into a polymer matrix by using blending solvent or by the reduction of metal salt dispersed in the polymeric matrix. Generally, the production of pre-made Au NPs occurs in liquids with two-step processes: producing the gold nanoparticles first and then adding them to the liquid polymer. A reproducible method to synthetize Au nanoparticles (NPs) into polydimethylsiloxane (PDMS) without any external reducing or stabilizing agent is a challenge. In this paper, a single-step method is proposed to synthetize nanoparticles (NPs) and at the same time to realize reproducible porous and bulk composites using laser ablation in liquid. With this single-step process, the gold nanoparticles are therefore produced directly in the liquid polymer. The optical properties of the suspensions of AuNPs in distilled water and in the curing agent have been analyzed by the UV-VIS spectroscopy, employed in the transmission mode, and compared with those of the pure curing agent. The electrical dc conductivity of the porous PDMS/Au NPs nanocomposites has been evaluated by the I–V characteristics. Scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis have monitored the composition and morphology of the so-obtained composites and the size of the fabricated Au nanoparticles. Atomic force microscopy (AFM) has been used to determine the roughness of the bulk PDMS and its Au NP composites.


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