One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties

Crystal structure control is so important to the fluorescence properties that each element should be considered carefully. In conventional synthesis of ZnxCd[Formula: see text]S alloyed nanocrystals (NCs), most of studies focus on ligand–surface interaction on the formation of either zinc blende or wurtzite ZnxCd[Formula: see text]S nanocrystals, instead of the reactant source. In this work, mixed crystal phase was found easily in ZnxCd[Formula: see text]S alloyed NCs when reaction proceeded at high Zn/Cd source ratio condition. Therefore, we regulate the Zn/Cd ratio to obtain relative pure zinc-blende structure, and study the influence of structure change on the fluorescence properties. Further, we have proposed a two-step ZnS coating method to acquire ZnxCd[Formula: see text]S/ZnS NCs with separated crystal-phase between core and shell. Compared with maximum QY of 81% for ZnxCd[Formula: see text]S/ZnS NCs synthesized by conventional one-step coating method, the performance of the optimized NCs has significantly improved with maximum QY of 93%.

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Human mesenchymal stem cells derived from induced pluripotent stem cells down-regulate NK-cell cytolytic machinery

Blood ◽

10.1182/blood-2010-12-325324 ◽

2011 ◽

Vol 118 (12) ◽

pp. 3254-3262 ◽

Cited By ~ 93

Author(s):

Massimo Giuliani ◽

Noufissa Oudrhiri ◽

Zaeem M. Noman ◽

Amelia Vernochet ◽

Salem Chouaib ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Nk Cell ◽

Embryonic Stem ◽

Attractive Alternative ◽

Target Cells ◽

Alternative Source ◽

Activation Markers ◽

Induced Pluripotent

Abstract A major issue in immunosuppressive biotherapy is the use of mesenchymal stem cells (MSCs) that harbor regulatory capacity. However, currently used bone marrow-derived MSCs (BM-MSCs) are short-lived and cannot assure long lasting immunoregulatory function both in vitro and in vivo. Consequently, we have generated MSCs from human induced pluripotent stem (IPS-MSCs) cells that share similar properties with embryonic stem cells (ES-MSCs). Herein, we compared the immunoregulatory properties of ES/IPS-MSCs with those of BM-MSCs and showed, for the first time, that IPS-derived MSCs display remarkable inhibition of NK-cell proliferation and cytolytic function in a similar way to ES-MSCs. Both MSCs disrupt NK-cell cytolytic machinery in the same fashion that BM-MSCs, by down-regulating the expression of different activation markers and ERK1/2 signaling, leading to an impairment to form immunologic synapses with target cells and, therefore, secretion of cytotoxic granules. In addition, they are more resistant than adult BM-MSCs to preactivated NK cells. IPS-MSCs could represent an attractive alternative source of immunoregulatory cells, and their capacity to impair NK-cell cytotoxicity constitutes a complex mechanism to prevent allograft rejection.

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Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1

RSC Advances ◽

10.1039/c9ra07397c ◽

2019 ◽

Vol 9 (67) ◽

pp. 39381-39393 ◽

Cited By ~ 2

Author(s):

Jiacheng Yu ◽

Tongwei Zhang ◽

Huangtao Xu ◽

Xiaoli Dong ◽

Yao Cai ◽

...

Keyword(s):

Iron Oxide ◽

Nanoparticle Synthesis ◽

Iron Oxide Nanoparticle ◽

One Step ◽

Oxide Nanoparticle ◽

Pyrococcus Yayanosii

A novel thermostable ferritin from Pyrococcus yayanosii CH1 directs the synthesis of thermostable magnetoferritins (M-PcFn5000) in one step.

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One Step Core-Shell Silicon/Carbon Nanoparticle Synthesis By Laser Pyrolysis: Application to Anode Material in Lithium-Ion Batteries

ECS Meeting Abstracts ◽

10.1149/ma2014-02/5/331 ◽

2014 ◽

Keyword(s):

Lithium Ion Batteries ◽

Anode Material ◽

Nanoparticle Synthesis ◽

Lithium Ion ◽

Core Shell ◽

Carbon Nanoparticle ◽

Laser Pyrolysis ◽

Silicon Carbon ◽

One Step

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Fabrication and in vitro biocompatibilities of fibrous biocomposites consisting of PCL and M13 bacteriophage-conjugated alginate for bone tissue engineering

Journal of Materials Chemistry B ◽

10.1039/c5tb01748c ◽

2016 ◽

Vol 4 (4) ◽

pp. 656-665 ◽

Cited By ~ 6

Author(s):

Jae Yoon Lee ◽

Jinhyo Chung ◽

Woo-Jae Chung ◽

GeunHyung Kim

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tissue Regeneration ◽

Hard Tissue ◽

M13 Bacteriophage ◽

Bioactive Component ◽

M13 Phage

We propose a new biocomposite consisting of M13-phage-conjugated alginate and PCL micro/nanofibers, and it has potential to be used as an excellent bioactive component for hard tissue regeneration.

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Efficient Synthesis of Simvastatin by Use of Whole-Cell Biocatalysis

Applied and Environmental Microbiology ◽

10.1128/aem.02820-06 ◽

2007 ◽

Vol 73 (7) ◽

pp. 2054-2060 ◽

Cited By ~ 63

Author(s):

Xinkai Xie ◽

Yi Tang

Keyword(s):

High Performance ◽

Fermentation Broth ◽

Attractive Alternative ◽

Acyl Donor ◽

Whole Cell ◽

Whole Cell Biocatalysis ◽

Cholesterol Levels ◽

Fed Batch Fermentation ◽

One Step ◽

Semisynthetic Derivative

ABSTRACT Simvastatin is a semisynthetic derivative of the fungal polyketide lovastatin and is an important drug for lowering cholesterol levels in adults. We have developed a one-step, whole-cell biocatalytic process for the synthesis of simvastatin from monacolin J. By using an Escherichia coli strain overexpressing the previously discovered acyltransferase LovD (X. Xie, K. Watanabe, W. A. Wojcicki, C. C. Wang, and Y. Tang, Chem. Biol. 13:1161-1169, 2006), we were able to achieve >99% conversion of monacolin J to simvastatin without the use of any chemical protection steps. The key finding was a membrane-permeable substrate, α-dimethylbutyryl-S-methyl-mercaptopropionate, that was efficiently utilized by LovD as the acyl donor. The process was scaled up for gram-scale synthesis of simvastatin. We also demonstrated that simvastatin synthesized via this method can be readily purified from the fermentation broth with >90% recovery and >98% purity as determined by high-performance liquid chromatography. Bioconversion using high-cell-density, fed-batch fermentation was also examined. The whole-cell biocatalysis can therefore be an attractive alternative to currently used multistep semisynthetic transformations.

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Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Irradiated Bone Marrow-Derived Mesenchymal Stem Cells

Journal of Bone Biology and Osteoporosis ◽

10.18314/jbo.v4i1.1230 ◽

2018 ◽

Vol 4 (1) ◽

pp. 94-98

Author(s):

Yumiko Kawata ◽

Eiji Ikami ◽

Junya Nojima ◽

Shoichiro Kokabu ◽

Tetsuya Yoda ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Radiation Dose ◽

Mrna Expression ◽

Attractive Alternative ◽

Total Radiation ◽

Alternative Source ◽

Murine Bone Marrow ◽

Total Radiation Dose

Adipose-derived Mesenchymal stem cells have emerged as an attractive alternative source of cell therapy. While radiation therapy is an important application for head and neck cancer, the effect of adipose-derived mesenchymal stem cells on irradiated bone marrow-derived Mesenchymal stem cells is still unclear. Herein, we explored how clinical total radiation dose affect gene expression related with differentiation on murine bone marrow-derived mesenchymal stem cells and how murine adipose-derived mesenchymal stem cells affect irradiated murine bone marrow-derived mesenchymal stem cells. The clinical total radiation dose upregulates osterix mRNA expression. Moreover, adiposederived mesenchymal stem cells dramatically promoted the upregulation of osterix mRNA expression whereas inhibited NFATc1 mRNA expression. Taken as a whole, irradiated bone marrow-derived mesenchymal stem cells co-cultured with adipose-derived mesenchymal stem cells may exhibit osteogenic property.

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Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications

Cellular & Molecular Biology Letters ◽

10.2478/s11658-013-0101-4 ◽

2013 ◽

Vol 18 (4) ◽

Cited By ~ 34

Author(s):

Izabela Harasymiak-Krzyżanowska ◽

Alicja Niedojadło ◽

Jolanta Karwat ◽

Lidia Kotuła ◽

Paulina Gil-Kulik ◽

...

Keyword(s):

Stem Cells ◽

Adipose Tissue ◽

Regenerative Medicine ◽

Stromal Cells ◽

Pluripotent Stem Cells ◽

Sampling Procedure ◽

Patient Comfort ◽

Attractive Alternative ◽

Isolated Cells ◽

Alternative Source

AbstractThe stromal-vascular cell fraction (SVF) of adipose tissue can be an abundant source of both multipotent and pluripotent stem cells, known as adipose-derived stem cells or adipose tissue-derived stromal cells (ADSCs). The SVF also contains vascular cells, targeted progenitor cells, and preadipocytes. Stromal cells isolated from adipose tissue express common surface antigens, show the ability to adhere to plastic, and produce forms that resemble fibroblasts. They are characterized by a high proliferation potential and the ability to differentiate into cells of meso-, ecto- and endodermal origin. Although stem cells obtained from an adult organism have smaller capabilities for differentiation in comparison to embryonic and induced pluripotent stem cells (iPSs), the cost of obtaining them is significantly lower. The 40 years of research that mainly focused on the potential of bone marrow stem cells (BMSCs) revealed a number of negative factors: the painful sampling procedure, frequent complications, and small cell yield. The number of stem cells in adipose tissue is relatively large, and obtaining them is less invasive. Sampling through simple procedures such as liposuction performed under local anesthesia is less painful, ensuring patient comfort. The isolated cells are easily grown in culture, and they retain their properties over many passages. That is why adipose tissue has recently been treated as an attractive alternative source of stem cells. Essential aspects of ADSC biology and their use in regenerative medicine will be analyzed in this article.

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Improvement of High Affinity and Selectivity on Biosensors Using Genetically Engineered Phage by Binding Isotherm Screening

Viruses ◽

10.3390/v11030248 ◽

2019 ◽

Vol 11 (3) ◽

pp. 248 ◽

Cited By ~ 2

Author(s):

Jong-Min Lee ◽

Eun Choi ◽

Juyun Park ◽

Vasanthan Devaraj ◽

ChunTae Kim ◽

...

Keyword(s):

Screening Method ◽

Target Material ◽

Genetically Engineered ◽

Titration Calorimetry ◽

Binding Affinities ◽

Binding Model ◽

Screening Process ◽

M13 Bacteriophage ◽

Bacteriophage M13 ◽

M13 Phage

The genetically engineered M13 bacteriophage (M13 phage), developed via directed evolutionary screening process, can improve the sensitivity of sensors because of its selective binding to a target material. Herein, we propose a screening method to develop a selective and sensitive bioreporter for toxic material based on genetically engineered M13 phage. The paraquat (PQ)-binding M13 phage, developed by directed evolution, was used. The binding affinities of the PQ-binding M13 phage to PQ and similar molecules were analyzed using isothermal titration calorimetry (ITC). Based on the isotherms measured by ITC, binding affinities were calculated using the one-site binding model. The binding affinity was 5.161 × 10−7 for PQ, and 3.043 × 10−7 for diquat (DQ). The isotherm and raw ITC data show that the PQ-binding M13 phage does not selectively bind to difenzoquat (DIF). The phage biofilter experiment confirmed the ability of PQ-binding M13 bacteriophage to bind PQ. The surface-enhanced Raman scattering (SERS) platform based on the bioreporter, PQ-binding M13 phage, exhibited 3.7 times the signal intensity as compared with the wild-type-M13-phage-coated platform.

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