scholarly journals Reactive oxygen species (ROS) production in HepG2 cancer cell line through the application of localized alternating magnetic field

2020 ◽  
Vol 8 (34) ◽  
pp. 7667-7676 ◽  
Author(s):  
Alberto Sola-Leyva ◽  
Ylenia Jabalera ◽  
María A. Chico-Lozano ◽  
María P. Carrasco-Jiménez ◽  
Guillermo R. Iglesias ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Reactive Oxygen Species ◽  
Magnetic Fields ◽  
Cell Viability ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Cancer Cell Line ◽  
Ros Production ◽  
Oxygen Species ◽  
Alternating Magnetic Fields

The production of Reactive Oxygen Species after exposure of HepG2 cells to alternating magnetic fields can explain the loss of cell viability in spite of a negligible increase in temperature.

scholarly journals The Role of Water in the Effect of Weak Combined Magnetic Fields on Production of Reactive Oxygen Species (ROS) by Neutrophils

2020 ◽  
Vol 10 (9) ◽  
pp. 3326
Author(s):  
Vadim V. Novikov ◽  
Elena V. Yablokova ◽  
Evgeny E. Fesenko
Keyword(s):  
Magnetic Field ◽  
Reactive Oxygen Species ◽  
Magnetic Fields ◽  
Reactive Oxygen ◽  
Microwave Frequency ◽  
Ion Cyclotron Resonance ◽  
Ros Production ◽  
Oxygen Species ◽  
Hydronium Ion ◽  
Pre Treatment

Various models have been used to demonstrate the pronounced effects of the microwave frequency range electromagnetic fields, as well as weak and very weak combined collinear magnetic fields (CMF) with static and variable components in the order of micro- and nano-tesla. One of such models, previously shown to be sensitive to variations in the parameters of applied magnetic fields, is the neutrophil respiratory burst. Using luminol-enhanced cell chemiluminescence assay, we studied the effects of the CMF exerted directly on neutrophil suspensions and, indirectly, through aqueous solutions. To experimentally create a uniform standard weak CMF with 60 µT static and 100 nT alternating magnetic field components, we engineered a shielded magnetic field induction device. CMF applied directly to neutrophils enhanced reactive oxygen species (ROS) production by more than 36%. The pronounced stimulating effect was observed only when using the signals that included the frequency of 12.6 Hz that corresponds to the ion cyclotron resonance (ICR) frequency of the hydrated hydronium ion. Similarly, to direct exposure, CMF pre-treatment of a water sample subsequently added to the neutrophil suspension increased ROS production by 66%. The effect of CMF pre-treatment was retained after a series of dilutions and mechanical treatment but disappeared in “magnetic vacuum” or without mechanical influence. Therefore, weak and super weak magnetic fields may indirectly, via water, activate ROS production by neutrophils, provided that modulation of super weak component of collinear field corresponds to the ICR frequency of the hydrated hydronium ion.

scholarly journals Spin Biochemistry Modulates Reactive Oxygen Species (ROS) Production by Radio Frequency Magnetic Fields

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e93065 ◽  
Author(s):  
Robert J. Usselman ◽  
Iain Hill ◽  
David J. Singel ◽  
Carlos F. Martino
Keyword(s):  
Reactive Oxygen Species ◽  
Magnetic Fields ◽  
Radio Frequency ◽  
Reactive Oxygen ◽  
Ros Production ◽  
Oxygen Species

scholarly journals Reactive Oxygen Species in Host Plant Are Required for an Early Defense Response against Attack of Stagonospora nodorum Berk. Necrotrophic Effectors SnTox

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1586
Author(s):  
Svetlana Veselova ◽  
Tatyana Nuzhnaya ◽  
Guzel Burkhanova ◽  
Sergey Rumyantsev ◽  
Igor Maksimov
Keyword(s):  
Reactive Oxygen Species ◽  
Early Stage ◽  
Reactive Oxygen ◽  
Triticum Aestivum L ◽  
Redox Status ◽  
Stagonospora Nodorum ◽  
Ros Generation ◽  
Ros Production ◽  
Oxygen Species ◽  
Necrotrophic Effectors

Reactive oxygen species (ROS) play a central role in plant immune responses. The most important virulence factors of the Stagonospora nodorum Berk. are multiple fungal necrotrophic effectors (NEs) (SnTox) that affect the redox-status and cause necrosis and/or chlorosis in wheat lines possessing dominant susceptibility genes (Snn). However, the effect of NEs on ROS generation at the early stages of infection has not been studied. We studied the early stage of infection of various wheat genotypes with S nodorum isolates -Sn4VD, SnB, and Sn9MN, carrying a different set of NE genes. Our results indicate that all three NEs of SnToxA, SnTox1, SnTox3 significantly contributed to cause disease, and the virulence of the isolates depended on their differential expression in plants (Triticum aestivum L.). The Tsn1–SnToxA, Snn1–SnTox1and Snn3–SnTox3 interactions played an important role in inhibition ROS production at the initial stage of infection. The Snn3–SnTox3 inhibited ROS production in wheat by affecting NADPH-oxidases, peroxidases, superoxide dismutase and catalase. The Tsn1–SnToxA inhibited ROS production in wheat by affecting peroxidases and catalase. The Snn1–SnTox1 inhibited the production of ROS in wheat by mainly affecting a peroxidase. Collectively, these results show that the inverse gene-for gene interactions between effector of pathogen and product of host sensitivity gene suppress the host’s own PAMP-triggered immunity pathway, resulting in NE-triggered susceptibility (NETS). These results are fundamentally changing our understanding of the development of this economical important wheat disease.

scholarly journals CBIO-07. CELL DEATH INDUCED BY AN OSCILLATING MAGNETIC FIELD IN PATIENT DERIVED GLIOBLASTOMA CELLS IS MEDIATED BY REACTIVE OXYGEN SPECIES

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii17-ii17
Author(s):  
Shashank Hambarde ◽  
Martyn Sharpe ◽  
David Baskin ◽  
Santosh Helekar
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Cell Viability ◽  
Cortical Neurons ◽  
Reactive Oxygen ◽  
Great Promise ◽  
Antioxidant Vitamin ◽  
Ros Generation ◽  
Oxygen Species ◽  
Novel Therapy

Abstract Noninvasive cancer therapy with minimal side effects would be ideal for improving patient outcome in the clinic. We have developed a novel therapy using strong rotating magnets mounted on a helmet. They generate oscillating magnetic fields (OMF) that penetrate through the skull and cover the entire brain. We have demonstrated that OMF can effectively kill patient derived glioblastoma (GBM) cells in cell culture without having cytotoxic effects on cortical neurons and normal human astrocytes (NHA). Exposure of GBM cells to OMF reduced the cell viability by 33% in comparison to sham-treated cells (p< 0.001), while not affecting NHA cell viability. Time lapse video-microscopy for 16 h after OMF exposure showed a marked elevation of mitochondrial reactive oxygen species (ROS), and rapid apoptosis of GBM cells due to activation of caspase 3. Addition of a potent antioxidant vitamin E analog Trolox effectively blocked OMF-induced GBM cell death. Furthermore, OMF significantly potentiated the cytotoxic effect of the pro-oxidant Benzylamine. The results of our studies demonstrate that OMF-induced cell death is mediated by ROS generation. These results demonstrate a potent oncolytic effect on GBM cells that is novel and unrelated to any previously described therapy, including a very different mechanism of action and different technology compared to Optune therapy. The effect is very powerful, and unlike Optune, can be seen within hours after initiation of treatment. We believe that this technology holds great promise for new, effective and nontoxic treatment of glioblastoma.

scholarly journals Production and scavenging of reactive oxygen species both affect reproductive success in male and female Drosophila melanogaster

2021 ◽  
Author(s):  
Biz R. Turnell ◽  
Luisa Kumpitsch ◽  
Klaus Reinhardt
Keyword(s):  
Reactive Oxygen Species ◽  
Drosophila Melanogaster ◽  
Reactive Oxygen ◽  
Cellular Aging ◽  
Ros Production ◽  
Oxygen Species ◽  
Wild Type ◽  
Ros Scavenging ◽  
Respiratory Pathway ◽  
Male And Female

AbstractSperm aging is accelerated by the buildup of reactive oxygen species (ROS), which cause oxidative damage to various cellular components. Aging can be slowed by limiting the production of mitochondrial ROS and by increasing the production of antioxidants, both of which can be generated in the sperm cell itself or in the surrounding somatic tissues of the male and female reproductive tracts. However, few studies have compared the separate contributions of ROS production and ROS scavenging to sperm aging, or to cellular aging in general. We measured reproductive fitness in two lines of Drosophila melanogaster genetically engineered to (1) produce fewer ROS via expression of alternative oxidase (AOX), an alternative respiratory pathway; or (2) scavenge fewer ROS due to a loss-of-function mutation in the antioxidant gene dj-1β. Wild-type females mated to AOX males had increased fecundity and longer fertility durations, consistent with slower aging in AOX sperm. Contrary to expectations, fitness was not reduced in wild-type females mated to dj-1β males. Fecundity and fertility duration were increased in AOX and decreased in dj-1β females, indicating that female ROS levels may affect aging rates in stored sperm and/or eggs. Finally, we found evidence that accelerated aging in dj-1β sperm may have selected for more frequent mating. Our results help to clarify the relative roles of ROS production and ROS scavenging in the male and female reproductive systems.

Sign in / Sign up

Export Citation Format

Share Document