High-throughput microfluidic compressibility cytometry using multi-tilted-angle surface acoustic wave

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yanqi Wu ◽  
Alastair Stewart ◽  
Peter Vee-Sin Lee
Keyword(s):  
Mechanical Properties ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
High Throughput ◽  
Mechanical Measurement ◽  
Cellular Processes ◽  
Tilted Angle

Cellular mechanical properties (e.g. compressibility) are important biophysical markers in relation to cellular processes and functionality. Among the methods for cell mechanical measurement, acoustofluidic methods appear to be advantageous due...

scholarly journals An acoustic platform for single-cell, high-throughput measurements of the viscoelastic properties of cells

2020 ◽  
Author(s):  
Valentin Romanov ◽  
Giulia Silvani ◽  
Huiyu Zhu ◽  
Charles D Cox ◽  
Boris Martinac
Keyword(s):  
Mechanical Properties ◽  
Single Cell ◽  
High Throughput ◽  
Single Cells ◽  
Adherent Cells ◽  
Dependent Manner ◽  
Cellular Processes ◽  
Membrane Cytoskeleton ◽  
Change Temperature

ABSTRACTCellular processes including adhesion, migration and differentiation are governed by the distinct mechanical properties of each cell. Importantly, the mechanical properties of individual cells can vary depending on local physical and biochemical cues in a time-dependent manner resulting in significant inter-cell heterogeneity. While several different methods have been developed to interrogate the mechanical properties of single cells, throughput to capture this heterogeneity remains an issue. While new high-throughput techniques are slowly emerging, they are primarily aimed at characterizing cells in suspension, whereas high-throughput measurements of adherent cells have proven to be more challenging. Here, we demonstrate single-cell, high-throughput characterization of adherent cells using acoustic force spectroscopy. We demonstrate that cells undergo marked changes in viscoelasticity as a function of temperature, the measurements of which are facilitated by a closed microfluidic culturing environment that can rapidly change temperature between 21 °C and 37 °C. In addition, we show quantitative differences in cells exposed to different pharmacological treatments specifically targeting the membrane-cytoskeleton interface. Further, we utilize the high-throughput format of the AFS to rapidly probe, in excess of 1000 cells, three different cell-lines expressing different levels of a mechanosensitive protein, Piezo1, demonstrating the ability to differentiate between cells based on protein expression levels.

20512 Mechanical Properties of Thin Film Measured by Laser-Induced Surface Acoustic Wave Measurement

2007 ◽  
Vol 2007.13 (0) ◽  
pp. 207-208
Author(s):  
Megumi SASOU ◽  
Koji MIYAKE ◽  
Hideki TAKAGI ◽  
Ryutaro MAEDA ◽  
Shinya SASAKI
Keyword(s):  
Thin Film ◽  
Mechanical Properties ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Wave Measurement

A novel method of studying the micro-contact using surface acoustic wave sensor

Sensor Review ◽  
2016 ◽  
Vol 36 (4) ◽  
pp. 421-428 ◽  
Author(s):  
Jian Yang ◽  
Hejuan Chen
Keyword(s):  
Mechanical Properties ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Coupled System ◽  
Content Type ◽  
Saw Sensor ◽  
Micro Particles ◽  
Acoustic Wave Sensor ◽  
Coupling Stiffness ◽  
Novel Method

Purpose This paper aims to investigate the response behavior of the surface acoustic wave (SAW) sensor under the loading of micro-particles and to evaluate the feasibility of using the SAW sensor to study the micro-contact of the particle–plane interface. Design/methodology/approach An analytical perturbation theory of the coupled system of particle and SAW is presented. It shows that in the weak-coupling regime, the SAW sensor detects the coupling stiffness rather than the additional mass of the particle at the interface. The frequency perturbation formula expressed in parameters of the geometry and mechanical properties of the contact is further derived. The frequency shift of a 262-MHz Rayleigh-type SAW in the oscillation configuration under the loading of multiple starch particles of different sizes has been measured. Findings The experiment results of a linear relationship between the frequency increase and the sum of the radius of particles to the power of 2/3 verified the validity of the theory of linking the SAW response to the geometry and mechanical properties of the contact. Originality/value The SAW sensor could serve as a new candidate for studying the details of mechanical properties of the micro-contact of the interface.

scholarly journals A high-throughput acoustic cell sorter

Lab on a Chip ◽  
2015 ◽  
Vol 15 (19) ◽  
pp. 3870-3879 ◽  
Author(s):  
Liqiang Ren ◽  
Yuchao Chen ◽  
Peng Li ◽  
Zhangming Mao ◽  
Po-Hsun Huang ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
High Throughput ◽  
Cell Sorting ◽  
Cell Sorter

We developed a standing surface acoustic wave (SSAW)-based cell sorting device. The throughput of our device has been significantly improved by using focused interdigital transducers (FIDTs) as SSAW generator.

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