One-Pot Synthesized Organosilica Nanospheres for Multiplexed Fluorescent Nanobarcoding and Subcellular Tracking

Nanoscale ◽  
2022 ◽  
Author(s):  
Xinfeng Du ◽  
Yifu Wang ◽  
Jingying Zhai ◽  
Chao Guo ◽  
Yupu Zhang ◽  
...  
Keyword(s):  
One Pot ◽  

Multicolor microbeads are widely used in flow cytometry for various cellular and immunoassays. However, they are limited by the large size around one to tens of micrometer. Nanomaterials for multiplexed...

2013 ◽  
Vol 664 ◽  
pp. 59-65
Author(s):  
Pei Yong Guo ◽  
Jin Ming Wang ◽  
Ding Lu ◽  
Xiu Fu Hou

Flow cytometry was applied in the study of the groups and spatial distribution characteristics of the fine particles in Bantou—Shidou Reservoir, a subtropical reservoir in Xiamen,China. The fine organic autotrophic particles in reservoir could be divided into four groups conspicuously: R2 group in the smallest size with much phycoerythrin but little chlorophyll; R1 and R4 groups in relatively large size: R1 group with certain phycoerythrin and chlorophyll, while R4 group with no phycoerythrin but much chlorophyll; R3 group in the largest size with certain phycoerythrin and a large amount of chlorophyll. All the sampling stations in Shidou ( upper reaches) and Bantou ( lower reaches) reservoirs contain maximum R1 group with quantity between 35.41-46.85×106 ind/L and 23.39-43.02×106 ind/L; and minimum R3 group with quantity between 0.20-0.58×106 ind/L and 0.22-0.78×106 ind/L. The quantity of fine organic autotrophic particles is larger than that of organic azoic particles and inorganic particles. In view of the spatial distribution, R1 and R3 groups distribute unevenly in Shidou reservoir, while R2 and R4 groups change a little in quantity in different sampling stations and distribute much evenly. Moreover, in Bantou reservoir, R1, R2 and R3 groups distribute unevenly in the sampling stations, while R4 group distributes relatively evenly. The fast and multi-parameter detection capacity of Flow cytometry provides a much effective mean for the research on water fine organic autotrophic particles.


NANO ◽  
2014 ◽  
Vol 09 (01) ◽  
pp. 1450005 ◽  
Author(s):  
LUPING TANG ◽  
YONGHONG YE ◽  
HUICHAO ZHANG ◽  
CHEN LIAO ◽  
SHENFEI ZONG ◽  
...  

A series of monodisperse Ag nanoparticles ( Ag NPs), whose size can be tuned between 2 nm and 34 nm, have been successfully synthesized in one pot by controlling the growth time. The standard deviation of the small-size Ag NPs is 12% while that of the large-size ones is 15%. During the synthesis process, sodium borohydride and sodium dodecyl sulfate were used as reducing agent and surfactant, respectively, and the effect of the synthesis parameters on the size-tuning range and the size distribution of Ag NPs was studied to get the optimum growth condition. Moreover, surface enhanced Raman scattering (SERS) spectra were analyzed to explore the synthesis mechanism of Ag NPs.


2018 ◽  
Vol 42 (19) ◽  
pp. 15743-15749 ◽  
Author(s):  
Jing Wang ◽  
Daquan Li ◽  
Xinyue Liu ◽  
Yu Qiu ◽  
Xiao Peng ◽  
...  

N-Acetyl-l-cysteine-capped CdTe QDs with a large size (3.68 nm) are more suitable for detecting glutathione than the small ones (1.99 nm).


Author(s):  
R. A. Ricks ◽  
Angus J. Porter

During a recent investigation concerning the growth of γ' precipitates in nickel-base superalloys it was observed that the sign of the lattice mismatch between the coherent particles and the matrix (γ) was important in determining the ease with which matrix dislocations could be incorporated into the interface to relieve coherency strains. Thus alloys with a negative misfit (ie. the γ' lattice parameter was smaller than the matrix) could lose coherency easily and γ/γ' interfaces would exhibit regularly spaced networks of dislocations, as shown in figure 1 for the case of Nimonic 115 (misfit = -0.15%). In contrast, γ' particles in alloys with a positive misfit could grow to a large size and not show any such dislocation arrangements in the interface, thus indicating that coherency had not been lost. Figure 2 depicts a large γ' precipitate in Nimonic 80A (misfit = +0.32%) showing few interfacial dislocations.


Author(s):  
H. Weiland ◽  
D. P. Field

Recent advances in the automatic indexing of backscatter Kikuchi diffraction patterns on the scanning electron microscope (SEM) has resulted in the development of a new type of microscopy. The ability to obtain statistically relevant information on the spatial distribution of crystallite orientations is giving rise to new insight into polycrystalline microstructures and their relation to materials properties. A limitation of the technique in the SEM is that the spatial resolution of the measurement is restricted by the relatively large size of the electron beam in relation to various microstructural features. Typically the spatial resolution in the SEM is limited to about half a micron or greater. Heavily worked structures exhibit microstructural features much finer than this and require resolution on the order of nanometers for accurate characterization. Transmission electron microscope (TEM) techniques offer sufficient resolution to investigate heavily worked crystalline materials.Crystal lattice orientation determination from Kikuchi diffraction patterns in the TEM (Figure 1) requires knowledge of the relative positions of at least three non-parallel Kikuchi line pairs in relation to the crystallite and the electron beam.


Author(s):  
Patricia G. Calarco ◽  
Margaret C. Siebert

Visualization of preimplantation mammalian embryos by electron microscopy is difficult due to the large size of the ircells, their relative lack of internal structure, and their highly hydrated cytoplasm. For example, the fertilized egg of the mouse is a single cell of approximately 75μ in diameter with little organized cytoskelet on and apaucity ofor ganelles such as endoplasmic reticulum (ER) and Golgi material. Thus, techniques that work well on tissues or cell lines are often not adaptable to embryos at either the LM or EM level.Over several years we have perfected techniques for visualization of mammalian embryos by LM and TEM, SEM and for the pre-embedding localization of antigens. Post-embedding antigenlocalization in thin sections of mouse oocytes and embryos has presented a more difficult challenge and has been explored in LR White, LR Gold, soft EPON (after etching of sections), and Lowicryl K4M. To date, antigen localization has only been achieved in Lowicryl-embedded material, although even with polymerization at-40°C, the small ER vesicles characteristic of embryos are unrecognizable.


Author(s):  
K. Ohi ◽  
M. Mizuno ◽  
T. Kasai ◽  
Y. Ohkura ◽  
K. Mizuno ◽  
...  

In recent years, with electron microscopes coming into wider use, their installation environments do not necessarily give their performance full play. Their environmental conditions include air-conditioners, magnetic fields, and vibrations. We report a jointly developed entirely new vibration isolator which is effective against the vibrations transmitted from the floor.Conventionally, large-sized vibration isolators which need the digging of a pit have been used. These vibration isolators, however, are large present problems of installation and maintenance because of their large-size.Thus, we intended to make a vibration isolator which1) eliminates the need for changing the installation room2) eliminates the need of maintenance and3) are compact in size and easily installable.


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