scholarly journals Separation of collagen cyanogen bromide-derived peptides by reversed-phase high-performance liquid chromatography

1980 ◽  
Vol 191 (1) ◽  
pp. 253-256 ◽  
Author(s):  
M van der Rest ◽  
H P J Bennett ◽  
S Solomon ◽  
F H Glorieux
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Low Molecular Weight ◽  
Type I ◽  
Type Ii ◽  
Linear Gradient ◽  
Cnbr Cleavage ◽  
A New Technique

A new technique for separation of the CNBr cleavage products of collagen is described. It involves the use of a 30 nm pore reversed-phase high-performance liquid chromatography column eluted with a linear gradient of acetonitrile/water containing 0.01 M-heptafluorobutyric acid. The separation of type I, type II and type III CNBr peptides is described. Resolution is particularly good for the low-molecular-weight peptides. The method is fast, quantitative and sensitive, and the complete volatility of the eluent facilitates the recovery of the separated peptides.

scholarly journals Separation of porphyrin isomers by high-performance liquid chromatography

1983 ◽  
Vol 211 (2) ◽  
pp. 435-438 ◽  
Author(s):  
C K Lim ◽  
J M Rideout ◽  
D J Wright
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Type I ◽  
Phase Gradient ◽  
Preparative Isolation ◽  
Type Iii ◽  
Simultaneous Separation

A reversed-phase gradient elution system is described for the simultaneous separation of the type I and type III isomers of 8-, 7-, 6-, 5- and 4-carboxylated porphyrins and isocoproporphyrins. The method, adaptable for isocratic and stepwise separation of individual groups of isomers, is also suitable for preparative isolation of pure porphyrins. The analyses of porphyrin isomers in the urine and faeces of porphyric patients are examples of applications.

scholarly journals Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Kateřina Mikulíková ◽  
Oleg Romanov ◽  
Ivan Miksik ◽  
Adam Eckhardt ◽  
Statis Pataridis ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Free Amino ◽  
High Performance ◽  
Collagen Type ◽  
Polyacrylamide Gel Electrophoresis ◽  
Collagen Type I ◽  
Type I ◽  
Type Ii ◽  
Basic Representation

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α1) (from bovine) and collagen type I (α2) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.

scholarly journals ANALYSIS OF MIXED COMBINATIONS OF MEDICINAL PRODUCTS BASED ON ABAKAVIR, LAMIVUDIN, ZIDOVUDIN USING THE METHOD OF MICROCOLONIES LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 25 (3) ◽  
pp. 46-50
Author(s):  
YU. A. GONCHIKOVA ◽  
N. V. CHMELEVSKAYA ◽  
E. A. ILLARIONOVA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
High Performance ◽  
Lithium Perchlorate ◽  
Reversed Phase ◽  
Hplc Method ◽  
Medicinal Products ◽  
Linear Gradient ◽  
Detection And Separation

Aim.To develop a technique for the detection and separation of abacavir, lamivudine, zidovudine, both individually and combined with adaptol, azaleptin, analgin, amitriptyline, haloperidol, melipramine, neuleptil, phenobarbital, fluoxetine, and chlorprotixen using high performance liquid chromatography (HPLC).Materials and methods. A column filled with ProntoSIL-120-5-C18 AQ was used, eluting with A [0.2 M lithium perchlorate − 0.005 M perchloric acid] − water (5: 95); eluent B is acetonitrile. Chromatography conditions: a linear gradient of the solvent is 3700 μl from 5 to 70% at a flow rate of 100 μl / min. The temperature of the column was 40°C. The results were processed with the help of the computer program MultiChrome (ZAO Ampersend, Moscow).Results. The method of separation and detection of combinations of abacavir, lamivudine, zidovudine with adaptol, azaleptin, analgin, amitriptyline, haloperidol, melipramine, neuleptil, phenobarbital, fluoxetine and chlorprotixene by reversed-phase high-performance liquid chromatography was developed.Conclusion. The developed technique allows detecting abacavir, zidovudine, lamivudine with the help of the HPLC method after isolation from urine separately or at combined poisonings with adaptolom, azaleptinom, dipyrone, amitriptilinom, galoperidolom, melipraminom, neuleptilom, phenobarbital, fluoxetine and hlorprotiksenom

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